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Lee C.-T.,Zuoying Branch of Kaohsiung Armed Forces General Hospital | Chang L.-C.,I - Shou University | Wu P.-F.,National University of Kaohsiung
Inflammation | Year: 2016

This study explored that lipoic acid treatment for 24 h significantly upregulated and promoted heat shock-induced catalase expression and downregulated GPx1 messenger RNA (mRNA) expression, indicating that lipoic acid exhibits antioxidant activity in the decomposition of hydrogen peroxide by upregulating catalase expression. Moreover, lipoic acid treatment for 3 h increased and promoted heat shock-induced interleukin (IL)-6 mRNA and protein levels and that for 24 h downregulated IL-6 mRNA expression, suggesting a dual effect of lipoic acid on IL-6 regulation. Lipoic acid alone failed to increase or reduce tumor necrosis factor (TNF)-α mRNA and protein levels, whereas heat shock alone downregulated TNF-α mRNA and protein expression. These data suggest that lipoic acid does not have a proinflammatory role and that heat shock acts as an anti-inflammatory agent by downregulating TNF-α expression in C2C12 myotubes. Moreover, lipoic acid or heat shock alone upregulated the IL-6 receptor (IL-6R-α) and glycoprotein 130 (gp130) mRNA expression followed by IL-6 expression; these data indicate that the regulation of lipoic acid or heat shock is mediated by IL-6R signaling, thus suggesting that C2C12 myotubes possesses a mechanism for regulating IL-6R and gp130 expression following lipoic acid treatment or heat shock. © 2016, Springer Science+Business Media New York. Source


Liu W.-H.,National Sun Yat - sen University | Chen Y.-J.,National Sun Yat - sen University | Chien J.-H.,Zuoying Branch of Kaohsiung Armed Forces General Hospital | Chang L.-S.,National Sun Yat - sen University | Chang L.-S.,Kaohsiung Medical University
Journal of Cellular Physiology | Year: 2014

This study explores the suppression mechanism of amsacrine (4-(9-Acridinylamino)-N-(methanesulfonyl)-m-anisidine hydrochloride) on matrix metalloproteinase-2 (MMP-2) and MMP-9 expression in human leukemia cells. Amsacrine attenuated cell invasion with decreased MMP-2/MMP-9 protein expression and mRNA levels in U937, Jurkat, HL-60, K562, KU812, and MEG-01 cells. Moreover, amsacrine reduced both MMP-2/MMP-9 promoter luciferase activity and MMP-2/MMP-9 mRNA stability in leukemia cells. Studies on amsacrine-treated U937 cells revealed that amsacrine-elicited ROS generation induced JNK and p38 MAPK activation but reduced the phospho-ERK level. Amsacrine-induced ERK inactivation and p38 MAPK/JNK activation were demonstrated to suppress MMP-2/MMP-9 promoter luciferase activity and promote MMP-2/MMP-9 mRNA decay, respectively. p38 MAPK/JNK activation led to up-regulation of protein phosphatase 2A catalytic subunit α (PP2Acα) in amsacrine-treated U937 cells. Okadaic acid (PP2A inhibitor) treatment increased MMP-2/MMP-9 mRNA stability in amsacrine-treated cells, whereas PP2Acα over-expression increased MMP-2/MMP-9 mRNA decay. Amsacrine-induced MMP-2/MMP-9 down-regulation was also related to PP2Acα up-regulation on Jurkat, HL-60, K562, KU812, and MEG-01 cells. Collectively, our data indicate that amsacrine induces MMP-2/MMP-9 down-regulation via simultaneous suppression of genetic transcription and mRNA stability in human leukemia cells. © 2013 Wiley Periodicals, Inc. Source


Tsai W.-C.,Zuoying Branch of Kaohsiung Armed Forces General Hospital | Chen J.-C.,Taoyuan General Hospital | Tsao Y.-T.,Taoyuan General Hospital
American Journal of Emergency Medicine | Year: 2015

Pseudosubarachnoid hemorrhage is a unique appearance of hyperdensity on the computed tomographic scan without blood component in the subarachnoid spaces. It is usually seen in patients with severe brain edema and could be easily mistaken as a hemorrhagic emergency, leading to inappropriate invasive investigation.We describe a case with lithium intoxication in which fatal dialysis disequilibrium syndrome supervened, reflecting the ominous nature of pseudosubarachnoid hemorrhage. This report profiles imaging features, therapeutic strategies, and pathogenetic hypothesis and reinforces the need for physicians to maintain heightened vigilance of this manifestation. Source


Sun L.-M.,Zuoying Branch of Kaohsiung Armed Forces General Hospital | Lin C.-L.,Data Management | Lin C.-L.,China Medical University at Taichung | Chung C.-J.,China Medical University at Taichung | And 3 more authors.
European Journal of Neurology | Year: 2014

Background and purpose: Studies have suggested that multiple sclerosis (MS) might be linked to an overall reduced cancer rate, but a positive relationship is also found for several types of cancer. This study determines whether MS is associated with cancer risk in Taiwan. Methods: Data from the National Health Insurance System of Taiwan were used to assess this issue. The MS cohort included 1292 patients, and each patient was randomly frequency-matched with four participants without MS, based on their age, sex and index year (control cohort). Cox proportional hazards regression analysis was conducted to estimate the influence of MS on cancer risk. Results: A significantly higher risk of developing overall cancer was found amongst the MS cohort compared with the control cohort [adjusted hazard ratio (HR) 1.85, 95% confidence interval (CI) 1.26-2.74], as well as breast cancer (adjusted HR 2.23, 95% CI 1.11-4.46). Conclusions: The nationwide population-based cohort study revealed that Taiwanese patients with MS have a higher risk of developing overall cancer types and breast cancer in particular. © 2013 The Author(s) European Journal of Neurology © 2013 EFNS. Source


Tseng W.-S.,Zuoying Branch of Kaohsiung Armed Forces General Hospital | Tseng W.-S.,National Defense Medical Center
Medical Hypotheses | Year: 2014

Stroke is a medical emergency and can cause permanent neurological disability and death. Halitosis (bad breath) has been reported in many stroke patients. We speculated that this phenomenon may be present before as well as after a stroke. Moreover, many studies have shown that most of the causes of halitosis are also associated with an increased risk of stroke. Therefore, we hypothesized that the severity of halitosis could be a predictor of stroke. We hope that this hypothesis can be confirmed by further investigations, and that if confirmed it can be used as a tool to monitor disease control and/or early diagnosis and intervention of newly developed diseases related to both halitosis and stroke. © 2014 Elsevier Ltd. Source

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