Dalian, China

Zunyi Medical University

Dalian, China
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A method of ultra high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry was developed for the simultaneous quantification of 11 sesquiterpene lactones in 11 Jerusalem artichoke leaf samples harvested in a number of areas at different periods. The optimal chromatographic conditions were achieved on a ZORBAX Eclipse Plus C18 column (3.0 × 150 mm, 1.8 μm) with linear gradient elution of methanol and water in 8 min. Quantitative analysis was carried out under selective ion monitoring mode. All of the sesquiterpene lactones showed good linearity (R2 ≥ 0.9949), repeatability (relative standard deviations < 4.66%), and intra- and interday precisions (relative standard deviations < 4.52%) with an accuracy of 95.24–104.84%. The recoveries measured at three concentration levels varied from 95.07 to 104.87% with relative standard deviations less than 4.9%. The limit of detection and limit of quantitation for this method were 0.89–5.05 and 1.12–44.33 ng/mL, respectively. The results showed that the contents of sesquiterpene lactones varied significantly in the Jerusalem artichoke leaf samples from different areas. Among them, the content of sesquiterpene lactones in the sample collected from Dalian, Liaoning province was the highest and the early flowering period was considered to be the optimal harvest time. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Luo X.,Zunyi Medical University | Yang J.,Wuhan University of Science and Technology
Journal of Sensors | Year: 2017

Water pollution detection is of great importance in water conservation. In this paper, the water pollution detection problems of the network and of the node in sensor networks are discussed. The detection problems in both cases of the distribution of the monitoring noise being normal and nonnormal are considered. The pollution detection problems are analyzed based on hypothesis testing theory firstly; then, the specific detection algorithms are given. Finally, two implementation examples are given to illustrate how the proposed detection methods are used in the water pollution detection in sensor networks and prove the effectiveness of the proposed detection methods. © 2017 Xu Luo and Jun Yang.

Gu W.-G.,Zunyi Medical University | Liu B.-N.,Zunyi Medical University | Yuan J.-F.,Huazhong Agricultural University
Journal of Drug Targeting | Year: 2015

Three integrase (IN) inhibitors have been approved by FDA for clinical treatment of Human Immunodeficiency Virus (HIV) infection. This stimulates more researchers to focus their studies on this target for anti-HIV drug development. Three steps regarding of IN activity have been validated for inhibitor discovery: strand transfer, 3′-terminal processing, and IN-lens epithelium-derived growth factor (LEDGF)/p75 interaction. Among them, IN-LEDGF/p75 interaction is a new target validated in recent years. Emergence of drug-resistant virus strains makes this target appealing to pharmacologists. Compared with the traditional screening methods such as AlphaScreen and cell-based screening developed for IN inhibitor discovery, virtual screening is a powerful technique in modern drug discovery. Here we summarized the recent advances of virtual-screening targeting IN-LEDFG/p75 interaction. The combined application of virtual screening and experiments in drug discovery against IN-LEDFG/p75 interaction sheds light on anti-HIV research and drug discovery. © 2014 Informa UK Ltd.

Gu W.-G.,Zunyi Medical University
Biomedicine and Pharmacotherapy | Year: 2014

The current therapy for the human immunodeficiency virus (HIV) infection is a combination of anti-HIV drugs targeting multiple steps of virus replication. The drugs for the acquired immunodeficiency syndrome (AIDS) treatment include reverse transcriptase inhibitors, protease inhibitors, fusion inhibitors, co-receptor inhibitor and the newly added integrase inhibitors. Raltegravir, elvitegravir and dolutegravir are the three Food and Drug Administration (FDA) approved integrase strand transfer inhibitors for clinical treatment of HIV infection. The addition of these integrase inhibitors benefits a lot to HIV infected patients. Although it is only seven years from the first integrase inhibitor, which was approved by FDA to now, multiple drug resistant HIV strains have emerged in clinical treatment. Most of the drug resistant virus strains are against raltegravir. Some are cross-resistant to elvitegravir. Dolutegravir is effective for suppression of the current drug resistant viruses. A number of clinical trials have been performed on the three integrase inhibitors. In this study, the application of the three integrase inhibitors in clinical treatment and the findings of drug resistance to integrase inhibitors are summarized. © 2014 Elsevier Masson SAS.

Wang Z.,National University of Defense Technology | Liao K.,Zunyi Medical University | Xiong J.,National University of Defense Technology | Zhang Q.,National University of Defense Technology
IEEE Signal Processing Letters | Year: 2014

This letter presents an automatic moving object detection method in image sequences captured from videos. While we focus on extracting moving objects throughout a frame sequence, our approach does not require any prior knowledge such as the background modeling nor the interaction by users such as empirical thresholds tuning. Based on the continuous symmetric difference of the adjacent frames, we get the full resolution saliency map of the current frame, which highlights the moving objects with higher saliency values and meanwhile inhibits the saliency of the background. Then, the maximum entropy sum method is utilized to adaptively calculate the threshold to determine the candidate areas and get the reasonable attention seeds. After that, the ground truth is obtained based on the modified fuzzy growing of the attention seeds. The proposed algorithm is effective, robust and the experimental results demonstrate that it is promising for moving object detection. © 2013 IEEE.

Gu W.-G.,Zunyi Medical University
Trends in Biotechnology | Year: 2015

Genome editing (GE)-based HIV therapy is achieved by modification of infection-related genes to produce HIV-resistant cells followed by reinfusion of the modified cells into patients. The ultimate goal is to achieve a functional or actual cure for HIV infection. Despite multiple potential targets for GE-based HIV therapies, CCR5 is the most feasible owing to the naturally existing CCR5 δ32 genotype which confers resistance to HIV. A recent clinical trial of infusion of modified autologous CD4+ T cells proved safety and efficacy within the limits of the studies. However, long-term evaluation of the safety and efficacy is required before GE-based HIV therapy is ready for clinical implementation. © 2014 Elsevier Ltd.

Gu W.-G.,Zunyi Medical University | Chen X.-Q.,Zunyi Medical University
European Journal of Clinical Microbiology and Infectious Diseases | Year: 2014

Highly active antiretroviral therapy (HAART) is the only approach for human immunodeficiency virus (HIV) infection treatment at present. Although HAART is effective in controlling the progression of infection, it is impossible to eradicate the virus from patients. The patients have to live with the virus. Alternative ways for the cure of HIV infection have been investigated. As the major co-receptor for HIV-1 infection, C-C motif chemokine receptor 5 (CCR5) is naturally an ideal target for anti-HIV research. The first CCR5 antagonist, maraviroc, has been approved for the treatment of HIV infection. Several other CCR5 antagonists are in clinical trials. CCR5 delta32 is a natural genotype, conferring resistance to CCR5 using HIV-1 strains. Gene therapy research targeting this mutant has been conducted for HIV infection treatment. A Berlin patient has been cured of HIV infection by the transplantation of stem cells from a CCR5 delta32 genotype donor. The infusion of an engineered zinc finger nuclease (ZFN)-modified autologous cluster of differentiation 4 (CD4) T cells has been proved to be a promising direction recently. In this study, the anti-HIV research targeting CCR5 is summarized, including CCR5 antagonist development, stem cell transplantation, and gene therapy. © 2014, Springer-Verlag Berlin Heidelberg.

Gu W.-G.,Zunyi Medical University | Zhang X.,Kunming Medical University | Yuan J.-F.,Huazhong Agricultural University
AAPS Journal | Year: 2014

Although highly active antiretroviral therapy (HAART) is effective in controlling the progression of AIDS, the emergence of drug-resistant strains increases the difficulty of successful treatment of patients with HIV infection. Increasing numbers of patients are facing the dilemma that comes with the running out of drug combinations for HAART. Computational methods play a key role in anti-HIV drug development. A substantial number of studies have been performed in anti-HIV drug development using various computational methods, such as virtual screening, QSAR, molecular docking, and homology modeling, etc. In this review, we summarize recent advances in the application of computational methods to anti-HIV drug development for five key targets as follows: reverse transcriptase, protease, integrase, CCR5, and CXCR4. We hope that this review will stimulate researchers from multiple disciplines to consider computational methods in the anti-HIV drug development process. © 2014 American Association of Pharmaceutical Scientists.

Liu Q.,Zunyi Medical University
Engineering Technology, Engineering Education and Engineering Management - International Conference on Engineering Technology, Engineering Education and Engineering Management, ETEEEM 2014 | Year: 2015

Objective: The STI characteristics and blood lactate concentration change law as well as the correlation in the discontinuous incremental load were researched in this paper, aiming at simplifying test procedures and changing the traumatic test to non-traumatic test in the future sports practice, and also providing a theoretical reference for sports trainings. Methods: In this experiment, 25 male students from the Department of Physical Education were chosen as tested objects. After these tested objects were confirmed to be without cardiovascular disease, STI during the quiet period was tested with MCA-4B cardiac function test system, and also blood lactate was tested through the fingertip blood taken by Young’s Improvement Method. Then, discontinuous incremental load exercise was finished on power bicycle: exercise load started from 60 W and increased 30 W at each level, and the rotate speed was 60 rotates per minute; the load at each level continued for 3 minutes, and the data were collected at an interval of sixty seconds after the load of each level was finished, but the data would be automatically cancelled if the interval was more than 60 seconds; the rotate speed kept unchanged until strength was exhausted. Results: First, in the discontinuous incremental load process, the STI change trend of the tested objects was as follows: the heart rate increased with the increase of the load, and the increase amplitude was different at different load stage but reached the largest in 150 W-180 W; the value of Q-U/LVET trended to decrease with the increase of the load first but increase after180 W; ICT, ICT/LVET and PEP/LVET trended to decrease first and then increase; other indexes trended to decrease with the increase of the load, and the decrease amplitude was different in different load but reached the largest in 150 W-180 W. Second, in the discontinuous incremental load process, the blood lactate concentrations of the tested objects in two groups trended to increase, and the increase amplitude of group II reached the largest in 150 W-180 W. Third, in the discontinuous incremental load process, the STI and blood lactate concentration of the tested objects in two groups not only changed regularly and also were correlated with each other; in this paper, the regression equation was established by choosing the heart rate (X1, times/min), left ventricular ejection time (X2, ms), and the mechanical systolic time (X3, ms) as independent variables and the blood lactate concentration change (Y, mol/L) as dependent variable: Y = 10.693 + 4.08 × X1 × 10-2+7.127 × X2 × 10-2-9.99X3 × 10-2. This equation reached the significant level (P < 0.05) after test, so it was of reliability. Conclusion: First, according to the research results of this experiment, the changes of STI in the discontinuous incremental load process fundamentally lied in the a series of cardiac function regular changes with group characteristics caused by the regular changes of heart rate and stroke volume. Second, in the discontinuous incremental load process, the STI and blood lactate concentration of the tested objects in two groups not only changed regularly and also were highly correlated with each other. © 2015 Taylor & Francis Group, London.

Zhou Y.,Zunyi Medical University | Xie Q.,Hunan Normal University
Sensors and Actuators, B: Chemical | Year: 2016

We have synthesized hyaluronic acid-coated magnetic nanoparticles (HA-MNPs) to selectively collect and detect of leukemia cells (CCRF-CEM) in combination with quartz crystal microbalance (QCM) measurement. Based on the specific binding of HA with CD44 receptors overexpressed on cell surface, HA-MNPs were successfully employed for selectively extracting CCRF-CEM cells from the complex matrices including human plasma samples. The collection and detection conditions for CCRF-CEM cells were optimized. Under optimized conditions, a detection limit of 8 × 103 cells mL-1 was obtained. The HA-MNPs-based magnetic separation combined with sensitive QCM measurement provides a simple, rapid and economical method for collecting and detecting leukemia cells, and this method may have great potential for wider applications in biomedical research and clinical diagnostics. © 2015 Elsevier B.V. All rights reserved.

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