Gu W.-G.,Zunyi Medical University |
Zhang X.,Kunming Medical University |
Yuan J.-F.,Huazhong Agricultural University
AAPS Journal | Year: 2014
Although highly active antiretroviral therapy (HAART) is effective in controlling the progression of AIDS, the emergence of drug-resistant strains increases the difficulty of successful treatment of patients with HIV infection. Increasing numbers of patients are facing the dilemma that comes with the running out of drug combinations for HAART. Computational methods play a key role in anti-HIV drug development. A substantial number of studies have been performed in anti-HIV drug development using various computational methods, such as virtual screening, QSAR, molecular docking, and homology modeling, etc. In this review, we summarize recent advances in the application of computational methods to anti-HIV drug development for five key targets as follows: reverse transcriptase, protease, integrase, CCR5, and CXCR4. We hope that this review will stimulate researchers from multiple disciplines to consider computational methods in the anti-HIV drug development process. © 2014 American Association of Pharmaceutical Scientists.
Liu W.,Peking University |
Zhou J.-G.,Zunyi Medical University |
Sun Y.,Peking University |
Zhang L.,Peking University |
Xing B.-C.,Peking University
Journal of Gastrointestinal Surgery | Year: 2015
Objective: Hepatic resection has been increasingly performed in patients with Barcelona Clinic Liver Cancer (BCLC) stage B hepatocellular carcinoma (HCC), but the current evidence supporting its efficacy remains controversial. The aim of this systematic review was to investigate the long-term survival and safety of hepatic resection compared to transarterial chemoembolization (TACE) alone for BCLC stage B HCC. Methods: Eligible trials that compared hepatic resection with TACE alone for intermediate HCC were identified from the Embase, PubMed, Web of Science, and Cochrane databases. The study outcomes included overall survival (OS) rate and treatment-related complication rate. Hazard ratios (HRs) with a 95 % confidence interval were used to measure the pooled effect according to a random-effects or fixed-effects model, depending on the heterogeneity among the included studies. The heterogeneity among these trials statistically was evaluated using the χ2 and I2 tests. Sensitivity analyses were also performed. Results: A total of 9 studies containing 4958 patients were included. The comparison between hepatic resection and TACE revealed a pooled HR for 3-year OS of 0.403 (95 % CI 0.364–0.446, p = 0.000; I2 = 0 %, p = 0.643) and a pooled HR for 5-year OS of 0.433 (95 % CI 0.394–0.475, p = 0.000; I2 = 0 %, p = 0.468). An AFP level >400 ng/ml and being HBV-positive were factors significantly correlated with overall survival. For treatment-related complications, the overall odds ratio (OR) for hepatic resection versus TACE was 0.990 (95 % CI 0.934–1.049; p = 0.728; I2 = 64.5 %, p = 0.060). Conclusion: Hepatic resection likely improved overall survival compared with TACE alone in BCLC stage B HCC patients, but did not increase the incidence of treatment-related complications. An AFP level >400 ng/ml and HBV positivity were significantly correlated with poor OS. © 2015, The Society for Surgery of the Alimentary Tract.
Zhou Y.,Zunyi Medical University |
Xie Q.,Hunan Normal University
Sensors and Actuators, B: Chemical | Year: 2016
We have synthesized hyaluronic acid-coated magnetic nanoparticles (HA-MNPs) to selectively collect and detect of leukemia cells (CCRF-CEM) in combination with quartz crystal microbalance (QCM) measurement. Based on the specific binding of HA with CD44 receptors overexpressed on cell surface, HA-MNPs were successfully employed for selectively extracting CCRF-CEM cells from the complex matrices including human plasma samples. The collection and detection conditions for CCRF-CEM cells were optimized. Under optimized conditions, a detection limit of 8 × 103 cells mL-1 was obtained. The HA-MNPs-based magnetic separation combined with sensitive QCM measurement provides a simple, rapid and economical method for collecting and detecting leukemia cells, and this method may have great potential for wider applications in biomedical research and clinical diagnostics. © 2015 Elsevier B.V. All rights reserved.
Liu Q.,Zunyi Medical University
Engineering Technology, Engineering Education and Engineering Management - International Conference on Engineering Technology, Engineering Education and Engineering Management, ETEEEM 2014 | Year: 2015
Objective: The STI characteristics and blood lactate concentration change law as well as the correlation in the discontinuous incremental load were researched in this paper, aiming at simplifying test procedures and changing the traumatic test to non-traumatic test in the future sports practice, and also providing a theoretical reference for sports trainings. Methods: In this experiment, 25 male students from the Department of Physical Education were chosen as tested objects. After these tested objects were confirmed to be without cardiovascular disease, STI during the quiet period was tested with MCA-4B cardiac function test system, and also blood lactate was tested through the fingertip blood taken by Young’s Improvement Method. Then, discontinuous incremental load exercise was finished on power bicycle: exercise load started from 60 W and increased 30 W at each level, and the rotate speed was 60 rotates per minute; the load at each level continued for 3 minutes, and the data were collected at an interval of sixty seconds after the load of each level was finished, but the data would be automatically cancelled if the interval was more than 60 seconds; the rotate speed kept unchanged until strength was exhausted. Results: First, in the discontinuous incremental load process, the STI change trend of the tested objects was as follows: the heart rate increased with the increase of the load, and the increase amplitude was different at different load stage but reached the largest in 150 W-180 W; the value of Q-U/LVET trended to decrease with the increase of the load first but increase after180 W; ICT, ICT/LVET and PEP/LVET trended to decrease first and then increase; other indexes trended to decrease with the increase of the load, and the decrease amplitude was different in different load but reached the largest in 150 W-180 W. Second, in the discontinuous incremental load process, the blood lactate concentrations of the tested objects in two groups trended to increase, and the increase amplitude of group II reached the largest in 150 W-180 W. Third, in the discontinuous incremental load process, the STI and blood lactate concentration of the tested objects in two groups not only changed regularly and also were correlated with each other; in this paper, the regression equation was established by choosing the heart rate (X1, times/min), left ventricular ejection time (X2, ms), and the mechanical systolic time (X3, ms) as independent variables and the blood lactate concentration change (Y, mol/L) as dependent variable: Y = 10.693 + 4.08 × X1 × 10-2+7.127 × X2 × 10-2-9.99X3 × 10-2. This equation reached the significant level (P < 0.05) after test, so it was of reliability. Conclusion: First, according to the research results of this experiment, the changes of STI in the discontinuous incremental load process fundamentally lied in the a series of cardiac function regular changes with group characteristics caused by the regular changes of heart rate and stroke volume. Second, in the discontinuous incremental load process, the STI and blood lactate concentration of the tested objects in two groups not only changed regularly and also were highly correlated with each other. © 2015 Taylor & Francis Group, London.
Dong H.,Zhengzhou University |
Li Q.,Zhengzhou University |
Zhang Y.,Zunyi Medical University |
Tan W.,Zunyi Medical University |
Jiang Z.,Anhui Medical University
PLoS ONE | Year: 2013
Purpose:The interleukin-23 receptor (IL-23R) has been shown to be associated with ankylosing spondylitis (AS) in many different populations. This study examined whether IL-23R polymorphisms were associated with susceptibility to this disease in a Chinese Han population.Methods:Three single-nucleotide polymorphisms (SNP), rs7517847, rs11209032, and rs17375018, were genotyped in 291 AS patients and 312 age-, sex-, and ethnically matched healthy controls using a polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) assay.Results:The genotype and allele frequencies of rs17375018, rs7517847, and rs11209032 were not different between the patients with AS and the healthy controls. On the one hand, stratification analysis indicated that the rs17375018 GG genotype and the G allele were increased in AS patients who were HLA-B27 positive (corrected p = 0.024, odds ratio [OR] 2.35, 95% CI 1.30-4.24; pc = 0.006, OR 1.98, 95% CI 1.28-3.07, respectively). On the other hand, the analysis according to clinical characteristics showed a significantly increased prevalence of the homozygous rs17375018 GG genotype and the G allele in patients with AS and uveitis compared with the controls (pc = 0.024 and pc = 0.024, respectively). In addition, haplotype analysis performed with the SHEsis platform revealed no significant difference concerning the haplotypes between AS patients and healthy controls.Conclusions:In this study, the results suggested that the rs17375018 of IL23R was positively associated with HLA-B27-positive AS and that the rs17375018 GG of IL-23R was associated with AS concomitant with uveitis. We found no evidence for an association between the other two SNPs of IL-23R and AS. © 2013 Dong et al.