Zunyi Medical College Zhuhai Campus

Zhuhai, China

Zunyi Medical College Zhuhai Campus

Zhuhai, China

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Feng L.-J.,Guangdong University of Technology | Feng L.-J.,Zunyi Medical College Zhuhai Campus | Guo J.-W.,Guangdong University of Technology | Zhong X.,Guangdong University of Technology | Sun Z.-Y.,Zunyi Medical College Zhuhai Campus
Journal of Macromolecular Science, Part A: Pure and Applied Chemistry | Year: 2014

Considering intrinsic properties of conjugated polyfluorenes and special functions of porous polymers, synthesis of fluorinated porous poly(spirobifluorene) via direct C-H arylation polycondensation is explored. Owing to the contorted structure and cross-linking nature, the obtained polymer FPSBF shows permanent porosities with Brunauer-Emmett-Teller specific surface area up to 700 m2 g-1 and exhibits a narrow pore size distribution with the dominant pore size at about 0.63 nm, which is more suitable for adsorption of small gas molecules. Based on the measured gas physisorption isotherms with pressure up to 1.13 bar, the obtained polymer shows good uptaking capacities for hydrogen (1.30 wt% at 1.0 bar and 77 K) and methane (4.80 wt% 1.0 bar and 273 K). Moreover, FPSBF has significant adsorption selectivity for CH4 against N2 and the estimated ideal adsorption selectivity ratio is up to 30/1 at 1.0 bar and 273 K, which makes the material possess potential application in gas separation. © 2014 Copyright Taylor & Francis Group, LLC.


Feng L.-J.,Guangdong University of Technology | Feng L.-J.,Zunyi Medical College Zhuhai Campus | Guo J.-W.,Guangdong University of Technology | Sun Z.-Y.,Zunyi Medical College Zhuhai Campus
Materials Letters | Year: 2014

Spirobixanthene-based polymeric monodisperse spheres with particle sizes about 600 nm in diameter have been prepared through surfactant-free Suzuki coupling polymerization in a single-phase. The polymer (PSBX) exhibits permanent microporous nature with high Brunauer-Emmett-Teller specific surface area up to 1020 m2 g-1. Its hydrogen storage can reach upto 1.67 wt% (1.0 bar and 77 K) and the uptake capacity for carbon dioxide is 12.0 wt% (1.0 bar and 273 K). Interestingly, PSBX is a highly hydrophobic material but with a high methanol uptake (574 mg g-1 at saturated vapor pressure and room temperature). The good sorption capacity of PSBX for toluene (887 mg g -1 at saturated vapor pressure and room temperature) makes it potent for use in the elimination of harmful small aromatic molecules in the environment. © 2013 Elsevier B.V.


Chen H.-X.,Zunyi Medical College Zhuhai Campus | Tang S.-P.,Shenzhen Childrens Hospital | Jiang X.-P.,Shenzhen Childrens Hospital | Cao J.,Shenzhen Childrens Hospital
Medicine (United States) | Year: 2014

In the traditional view, muscle atrophy and interstitial fibrosis were regarded as the basic pathological features of congenital muscular torticollis (CMT). But in the ultrastructure study, the mesenchyme- like cells, myoblasts, myofibroblasts, and fibroblasts were found in the proliferation of interstitium of CMT. To investigate the characteristics of pathological features and the mechanisms of muscle atrophy in CMT, we retrospectively reviewed the medical records of 185 CMT patients from July 2009 to July 2011 in Shenzhen Children's Hospital in China and performed pathological studies. According to age, the 185 CMT patients were divided into 4 groups. All resected surgical specimens were processed for hematoxylin and eosin staining and Masson trichromic staining. Sudan III staining was used for frozen sections, whereas immunohistochemical staining for S-100, calpain-1, ubiquitin, and 20S proteasome was carried out on 40 CMT specimens. Eight adductor muscle specimens from 8 patients with development dysplasia of the hip were taken as control group in the immunohistochemical staining. By Masson trichromic staining, the differences in the percent area of fibrous tissue in each CMT groups were significant. In Sudan III staining and immunostaining for S-100, adipocyte hyperplasia was the pathological feature of CMT. Moreover, compared with controls, most atrophic muscle fibers in CMT specimens were found to show strong immunoreactivity for calpain-1, ubiquitin, and 20S proteasome. With increasing age, fibrosis peaked at both sides and it was low in middle age group. Adipocytes increased with age. The characteristics of pathological features in CMT are changeable with age. The calpain and the ubiquitin-proteasome system may play a role in muscle atrophy of CMT. In the CMT, adipogenesis, fibrogenesis, and myogenesis may be the results of mesenchyme-like cells in SCM (sternocleidomastoid muscle). In conclusion, the present study furthermore supports maldevelopment of the fetal SCM theory for etiology of CMT. © 2014 Wolters Kluwer Health.


Liu K.S.,Border Armed Police Central Hospital of Guangdong Province | Fan X.Q.,Jinan University | Zhang L.,Zunyi Medical College | Wen Q.N.,Central Peoples Hospital of Zhanjiang | And 4 more authors.
Molecular Medicine Reports | Year: 2014

The current study aimed to investigate the rejection and survival time of grafted skin, and the changes of Treg cells, interleukin 10 (IL-10) and transforming growth factor-β (TGF-β) in peripheral blood following skin transplantation with recombinant human interleukin-10 (rhIL-10) or cyclosporin A (CsA), as well as the role of IL-10 in immunological rejection mechanisms. A total of 36 rabbits were divided into two groups. The skin of a donor rabbit was transplanted onto the back of one receptor rabbit. Receptors were randomly divided into six groups, including rhIL-10 low-dose (5 μg/kg/d), rhIL-10 high-dose (10 μg/kg/d), CsA low-dose (5 mg/kg/d), CsA high-dose (10 mg/kg/d), rhIL-10 (5 μg/kg/d) and CsA (5 mg/kg/d) and negative control normal saline (NS; 1 ml/d). All groups received intramuscular drug injection for ten days, beginning one day prior to skin transplantation surgery. Following transplantation, each rabbit's peripheral blood was collected at different times. The changes of CD4+CD25+ regulatory T cells, IL-10 and TGF-β were determined by flow cytometry and enzyme-linked immunosorbent assay. When compared with the control group, the rejection and survival times of the experimental groups were longer following skin graft. Compared with the two CsA groups and the control group, the proportion of CD4+CD25+ regulatory T cells of rhIL-10 groups was significantly upregulated on the 4th and 7th days following surgery. However, TGF-β levels were not significantly different. Data suggested that the concentration of IL-10 was positively correlated with the proportion of CD4+CD25+ regulatory T cells. In addition, IL-10 may delay the rejection time of rabbit skin transplantation and prolong the survival time. Thus, the role of IL-10 in inhibited allograft rejection may be associated with CD4+CD25+ regulatory T cells and IL-10, and may be independent of TGF-β.


Li M.,Shenyang Medical College | Li X.,The Peoples Hospital of Liaoning Province | Zhuang Y.,Tulane University | Wang Y.,Zunyi Medical College Zhuhai Campus | And 5 more authors.
Oncotarget | Year: 2016

The gene expression signatures of the molecular intrinsic subtypes of breast cancer are regulated by epigenetic mechanisms such as methylation of CpG islands in gene promoters. Epigenetic codes can be regulated by the tumor microenvironment. The Claudin-low subtype is associated with triple-negative invasive ductal carcinomas in patients. Herein we explored epigenetic regulation of gene expression in the Claudin-low breast cancer cells by extracellular matrix (ECM), a key component of the tumor microenvironment. We modeled attachment to ECM using laminin rich ECM three-dimensional organotypic culture (lrECM 3D). In 2D and lrECM 3D cultures we examined expression of the homeobox (HOX) genes that epigenetically regulated in development and cancer. We demonstrated induction of the selected HOX genes in lrECM 3D culture of the Claudin-low breast cancer cells MDA-MB-231 and Hs578T. In particular activation of HOXA9 expression in lrECM 3D culture required binding of bromodomain containing 4 to the HOXA9 promoter and involved CpG hypomethylation. Our findings warrant further investigation of the ECM-regulated epigenetic coding of gene expression in the Claudin-low breast cancer.


PubMed | Zunyi Medical College Zhuhai Campus, Xuzhou Medical College, Washington State University, Shenyang Medical College and 2 more.
Type: | Journal: Oncotarget | Year: 2016

The gene expression signatures of the molecular intrinsic subtypes of breast cancer are regulated by epigenetic mechanisms such as methylation of CpG islands in gene promoters. Epigenetic codes can be regulated by the tumor microenvironment. The Claudin-low subtype is associated with triple-negative invasive ductal carcinomas in patients. Herein we explored epigenetic regulation of gene expression in the Claudin-low breast cancer cells by extracellular matrix (ECM), a key component of the tumor microenvironment. We modeled attachment to ECM using laminin rich ECM three-dimensional organotypic culture (lrECM 3D). In 2D and lrECM 3D cultures we examined expression of the homeobox (HOX) genes that epigenetically regulated in development and cancer. We demonstrated induction of the selected HOX genes in lrECM 3D culture of the Claudin-low breast cancer cells MDA-MB-231 and Hs578T. In particular activation of HOXA9 expression in lrECM 3D culture required binding of bromodomain containing 4 to the HOXA9 promoter and involved CpG hypomethylation. Our findings warrant further investigation of the ECM-regulated epigenetic coding of gene expression in the Claudin-low breast cancer.


Lei S.-J.,Zunyi Medical College Zhuhai Campus | Shen Y.-X.,Interventional Imaging | Lou M.-W.,Interventional Imaging
Chinese Journal of Medical Imaging Technology | Year: 2011

MRI is noninvasive imaging technique that can display cerebral information. In recent years, there were many experimental reports about pathophysiological process of Wallerian degeneration after cerebral infarction, but these studies lacked visibility. The combination of various MR techniques can make the course of Wallerian degeneration visualization. The application of various MRI techniques on Wallerian degeneration after cerebral infarction, from imaging principle and pathogenesis, were reviewed in this article.


Wang W.,Zunyi Medical College Zhuhai Campus | Li W.,Yantai University | Li Q.,Yantai University
International Journal of Quantum Chemistry | Year: 2011

The lithium bond between HMgH and LiNH2 has been predicted and characterized with quantum chemical calculations at the MP2/6-311++G(d,p) level. Upon formation of the lithium bond, both the Mg-H and Li-N bonds are stretched. The Li-N bond undergoes a red shift, whereas the Mg-H bond exhibits a blue shift. The lithium-bonded complex is controlled mainly by electrostatic and polarization interactions. The binding energy of HMgH with LiNH2 is computed to be 12.47 kcal/mol. The binding of the two molecules is enhanced by the substitution with the methyl group in the Li acceptor, whereas it is weakened by the replacement with whether the electron-withdrawing group such as F, Cl, CN, NC, or the electron-donating group (OH and HN2). A negative cooperativity is present in the ternary system of 2LiNH2 and HMgH. The polarization interaction plays an important role in the negative cooperativity. © 2009 Wiley Periodicals, Inc.


Yu Y.-L.,Zunyi Medical College Zhuhai Campus | Tang Y.-C.,Zunyi Medical College Zhuhai Campus | Feng G.-W.,Zunyi Medical College Zhuhai Campus | Lan M.-B.,Zunyi Medical College Zhuhai Campus | And 3 more authors.
Acta Anatomica Sinica | Year: 2010

Objective: To study the effects of eucalyptus on embryonic development of rats. Methods: Totally 25 pregnant SD rats were randomly divided into 5 groups, each had 5 rats: three experiment groups (high, medium, low doses of eucalyptus), negative control group (peanut oil), positive control group (cyclophosphamide). Three experiment groups and negative control group were lavaged from gestation 10 to 14 days with eucalyptus in 100mg/(kg·d) , 200mg/(kg·d), 300mg/(kg·d) and peanut oil 2ml per animal per day respectively, while positive control group was ip with 12. 5mg/(kg·d) cyclophosphamide at gestation 13 days. All pregnant rats were sacrificed at the 19th day. The body, uterus, ovary and placenta weights of the pregnant rats were measured respectively. The numbers of absorbed, live and dead fetus were counted, respectively. The length for fetus body and tail as well as body weights were measured. Results: After administration of eucalyptus (100mg/kg,200mg/kg,300mg/kg) for 5 days, the body, uterus, ovary and placenta weights of pregnant rats showed no significant difference compared with negative control group (P > 0.05). The numbers for absorbed, live and dead fetus also showed no significant difference compared with negative control group (P > 0.05). All of the fetus body weights, body length and tail length were larger in the treated groups than those in the negative control group. But only in high doses of eucalyptus groups, the fetus weight of body showed difference compared with negative control group (P < 0.05) , others had no significant difference (P > 0.05). The fetus body weight, tail length in the positive control group were lower than those in the negative control group (P <0.05), but the fetus body length showed no significant difference compared with negative control group (P > 0.05). Conclusion: Under the experimental condition, eucalyptus showed no embryonic developmental toxicity, where cyclophosphamide showed embryonic developmental toxicity.


Yang Y.,South China University of Technology | Yang Y.,Zhejiang University | Yang Y.,Zunyi Medical College Zhuhai Campus | Huang L.,Zhejiang University | And 2 more authors.
Enzyme and Microbial Technology | Year: 2015

An FAD-dependent glucose dehydrogenase (FAD-GDH) from Aspergillus terreus NIH2624 was expressed in Escherichia coli with a yield of 228±16U/L of culture. Co-expression with chaperones DnaK/DnaJ/GrpE and osmotic stress induced by simple carbon sources enhanced productivity significantly, improving the yield to 23883±563U/L after optimization. FAD-GDH was purified in two steps with the specific activity of 604U/mg. Using d-glucose as substrate, the optimal pH and temperature for FAD-GDH were determined to be 7.5 and 50°C, respectively. Activity was stable across the pH range 3.5-9.0, and the half-life was 52min at 42°C. Km and Vmax were calculated as 86.7±5.3mM and 928±35U/mg, and the molecular weight was approximately 65.6kDa based on size exclusion chromatography, indicating a monomeric structure. The 3D structure of FAD-GDH was simulated by homology modelling using the structure of A. niger glucose oxidase (GOD) as template. From the model, His551, His508, Asn506 and Arg504 were identified as key residues, and their importance was verified by site-directed mutagenesis. Furthermore, three additional mutants (Arg84Ala, Tyr340Phe and Tyr406Phe) were generated and all exhibited a higher degree of substrate specificity than the native enzyme. These results extend our understanding of the structure and function of FAD-GDH, and could assist potential commercial applications. © 2014 Elsevier Inc.

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