Zhuhai Campus Zunyi Medical College

Zhuhai, China

Zhuhai Campus Zunyi Medical College

Zhuhai, China
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Lan M.-B.,Zhuhai Campus Zunyi Medical College | Li X.-H.,Zhuhai Campus Zunyi Medical College | Jiang H.-C.,Zhuhai Campus Zunyi Medical College | Zhang H.,Zhuhai Campus Zunyi Medical College | Yu Y.-L.,Zhuhai Campus Zunyi Medical College
Chinese Journal of Pharmacology and Toxicology | Year: 2010

OBJECTIVE: To explore whether volatile oil from Artemisia argyi has toxicity on skeleton of embryo mice. METHODS: Twenty-five Kunming pregnant mice were randomly divided into 5 groups : normal control group, ig given with peanut oil in equal volume; volatile oil 2, 1 and 0.5 ml·kg-1 groups, ig given volatile oil; and cyclophosphamide (positive control) group, ip given cyclophosphamide 12.5 mg·kg-1. The volatile group and normal control group were ig administrated once a day from the 12th day of pregnancy to the 16th day, for 5 d, while positive control group was ip given cyclophosphomide on the 13th day of pregnancy for 1 d. On the 16th day, the pregnant mice in each group were sacrificed to take out the embryo mice, and their body length and cauda length were measured, and half of the fetuses were observed for main skeleton development by double staining with alcian blue and alizari red S, scoring the forelimb buds with Neubert method. RESULTS: The body length was 18.9±0.4, 19.4±1.4 and (19.9±1.0) mm, respectively, and th cauda length was 7.8±0.2, 8.5±0.7 and (8.5±0.6) mm, respectively in volatile oil groups, compared with was body length (19.0±1.0) mm and caude length (7.9±1.1) mm in normal control group, there was no significant difference. There was also no difference in the Neubert score of the forelimb buds between in volatile oil groups(285.0±3.5, 278.0±4.1 and 289.4±2.6, respectively) and in normal control group(294.0±0.8). Also, compared with normal control group, main skeleton ossification of fetuses had no significant difference in volatile oil groups. Compared with normal control group and volatile oil groups, the body length and the cauda length were shortened dramatically by 21% and 23% in cyclophosphamide group. Compared with normal control group and volatile oil groups, the Neubert score of the forelimb buds and skeleton ossification of fetuses in cyclophosphamide group also showed significant difference (P < 0.05). CONCLUSION: Volatile oil from A. argyi 2, 1 and 0.5 ml·kg-1 have no toxicity on forelimb buds and skeleton of embryo mice.

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