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de Oliveira M.A.,Federal University of Amazonas | de Oliveira R.P.M.,Federal University of Amazonas | de Lima A.R.,University of Amazon | de Andrade E.V.,Federal University of Amazonas | And 2 more authors.
Revista Brasileira de Saude e Producao Animal | Year: 2017

This study aimed to identify proteins in the seminal plasma associated with fertility in sheep of Santa Inês in Manaus, AM, using twodimensional electrophoresis techniques associated with mass spectrometry. Semen samples from eight adult sheep were collected by removing an aliquot for the physical and morphological assessments of semen and seminal plasma was subjected to SDS-PAGE profile and two-dimensional electrophoresis. Gels were stained with colloidal Coomassie, scanned and analyzed using ImageMaster 2D Platinum software, version 6.0. The selected individual spots were cut from the master gel, digested with trypsin and subjected to identification by mass spectrometry (MALDI- Tof / Tof). Of the 108 spots detected in the gel, it selected 10 differential spots (based on the distribution thereof in the bidimensional gel and pre-analysis of the 2D ImageMaster Platinum Software) identifying 03 proteins: Clusterin, a protein 14-3-3 zeta chain and Ram Seminal versicles 22kDa Protein. The identity of these proteins implies that the components of seminal plasma participate in physiological processes involved in sperm protection, motility and sperm capacitation, all associated with fertility. These proteins need to be better studied to see whether the same could be used as molecular markers of fertility as they were also found in other studies conducted with sheep Santa Ines.

Malafaia P.,Federal Rural University of Rio de Janeiro | Cruz P.B.,Zootecnista | Silva V.P.,Federal Rural University of Rio de Janeiro
Pesquisa Veterinaria Brasileira | Year: 2014

Comparison of milk production and composition, body condition score and the cost of commercial or selective mineral supplementation was made using two groups of 16 lactating goats during 164 days. No significant difference in milk production between treatments was found. There was no effect of the type of mineral supplement on the composition of milk fat, protein and lactose as well as on the somatic cell count (SCC). The average of daily intake of the commercial supplement was 1.76 times greater than the supplement containing only sodium chloride associated with copper sulfate. The use of the selective mineral mixture based on clinical-nutritional examination of the animals led to a reduction of about 7 times the amount spent by mineral supplementation of this goat herd.

Costa P.M.,Federal University of Minas Gerais | Barbosa F.A.,Federal University of Minas Gerais | Alvarenga R.C.,Embrapa Milho e Sorgo | Guimaraes S.T.,Zootecnista | And 3 more authors.
Pesquisa Agropecuaria Brasileira | Year: 2017

The objective of this work was to evaluate the performance of crossbred steers post-weaned in an integrated crop-livestock system (ICL) and finished in a feedlot, fed either a high-level concentrate diet or whole corn with no roughage. Weaned calves of two breed groups - 1/2 Angus × 1/2 Nellore, and 1/2 Charolais × 1/4 Angus × 1/4 Nellore - were allotted in a completely randomized experimental design (CRD). During the rainy season, the 1/2 Angus × 1/2 Nellore animals showed a higher performance than the 1/2 Charolais × 1/4 Angus × 1/4 Nellore ones, with 0.748 and 0.490 kg average daily gain, respectively. The productivity in the post-weaning period was 926 kg ha-1 body weight in a 11-month period, in the ICL system. Subsequently, the animals were confined and fed high-concentrate diet or whole corn with no roughage, in a CRD with a 2×2 factorial arrangement. In the feedlot, the 1/2 Charolais × 1/4 Angus × 1/4 Nellore animals showed lower values for carcass average daily gain, carcass yield, and slaughter weight than the 1/2 Angus × 1/2 Nellore animals. The high productivity in the ICL shows that this strategy, associated with the use of crossbreeding during the post-weaning and finishing stages, can be indicated for the new demand for a sustainable livestock activity.

Backes A.A.,Federal University of Sergipe | Paulino M.F.,Federal University of Viçosa | Alves D.D.,Zootecnista | Valadares Filho S.C.,Federal University of Viçosa
Ciencia Rural | Year: 2010

Twelve steers have been used, when they had average live weight of 276kg, being four indubrasil and eight dairy crossbreeds (four crossbreeds HolsteinxGir and four HolsteinxGuzera). They have been weighted and distributed into two treatments (indubrasil and dairy crossbreeds), in phase fattening. The animals were studied up to 480kg of live weight. Grass hay Tifton 85 (Cynodon dactylon) have been used as feedstuff. After slaughtering samples of rumen, reticulum, omasum, abomasum and intestines have been collected after emptying and cleaning. Weight and samples values of blood, leather, feet, head, liver, kidneys, lung, tongue, spleen and internal fat (mesenterium plus perirenal and pericardia fats) have been collected. Empty body weight (EBW) have been obtained by summing weights of carcass, blood, leather, feet, tail, intestines and organs. The group head-foot-leather of indubrasil steers have been heaver than in dairy crossbreeds. The animals indubrasil and dairy crossbreeds, in fattening phase, have not presented significant differences for total weight of intestines and organs. The animals dairy crossbreeds present, in fattening phase, higher fat proportions intern than indubrasil animals.

Ricacheski S.T.,Federal Technological University of Paraná | Henrique D.S.,Federal Technological University of Paraná | Mayer L.R.R.,Federal Technological University of Paraná | de Oliveira J.G.,State University of Norte Fluminense | And 2 more authors.
Revista Brasileira de Saude e Producao Animal | Year: 2017

The present study aiming to determine the nutritional quality of oat (Avena sativa L.) IPR 126 in order to produce forage for ruminants. Four periods between harvests were used: 14, 21, 28 and 35 days, distributed in randomized blocks with four replicates for each treatment. The variables evaluated were: The concentration of dry matter (DM), neutral detergent insoluble fiber (NDF), crude potein (CP), lignin, dry matter (DM), ash, ether extract (EE) and in vitro digestibility of dry matter obtained by measurement of gas production. The profile of gas production was adjusted to the logistic bicompartimental mathematical model. The variables and the parameters of the adjusted gas production curves were analyzed as repeated measurements through the PROC MIXED of SAS (version 9.0) and the restricted maximum likelihood (REML) as the method of estimation of parameters. Regression analysis was performed for the variables: DM, CP, EE, NDF, ash, and for the parameter k2 of the bicompartimental model. DM and NDF concentrations increased linearly, CP, ash, and the estimations of the parameter k2 reduced linearly and the EE concentration showed a cubic behavior in function of the age of harvest. Lignin and other parameters of the Schofield model were not influenced by the age of harvest. The harvest interval influenced some chemical components and degradation rate of fiber carbohydrates, but do not interfere in lignin concentration. The forage with 21 days of cutting interval has the high nutritional value.

Da Cruz R.S.,Federal University of Tocantins | Alexandrino E.,Zootecnista | Missio R.L.,Zootecnista | Restle J.,Engenheiro Agronomo | And 4 more authors.
Bioscience Journal | Year: 2015

The objective was to evaluate the carcass characteristics of feedlot bulls fed levels of concentrate (65 and 71%) with or without the inclusion of 35% of babassu mesocarp bran (BMB) in concentrate fraction of the diet. Twenty eight young bulls were used, with initial age of 21 month and 356.7±19.2 kg of initial body weight. The experimental design was completely randomized design with treatments in a factorial arrangement 2x2. Hot and cold carcass weights were not affected by dietary concentrate level and the inclusion of BMB in the concentrate, with average values of 256.39 and 245.87 kg, respectively. Hot and cold carcass yield were lower for diets that associated the lower level of concentrate and 35% of BMB in the concentrate fraction of the diet. Subcutaneous fat thickness, conformation and carcass physiological maturity were not affected by concentrate level and the inclusion of the BMB, averaging 3.6 mm, 10.89 and 12.93 points respectively. The inclusion of 35% of BMB in the concentrate associated with different levels of concentrates did not alter commercial cuts weight. The inclusion of 35% of the BMB in the concentrate fraction of diets with high concentrate for feedlot bulls does not change the carcass characteristics of economic interest, providing production of carcasses with adequate quality. © 2015, Biosci. J. All rights reserved.

de Oliveira L.B.T.,Federal University of Tocantins | dos Santos A.C.,Federal University of Tocantins | Lima J.S.,Zootecnista | Neves Neto D.N.,Federal University of Tocantins
Revista Brasileira de Saude e Producao Animal | Year: 2015

This work aimed to evaluate the spatial variability of structural and productive responses of Marandu grass depending on the chemical characteristics of the soil and topography. The experiment was conducted in a topossequence, school of Veterinary Medicine and Animal Science, The V variables were evaluated CTC, SB, m, pH at a depth of 0-20 cm, effective depth, number of tillers (m2), production dry matter (kg ha-1), when sward (cm). 72 samples were collected in georeferenced points on sloping ground. The soil was classified as Entisol in the upper portion (top), Udorthent Eutrophic the upper third of the slope (shoulder), Udorthent eutrophic in the middle third of the slope (halfslope) and Quartzips amment Hydromorphic in the lower third (pedimento). All variables presented spatial dependence with variograms of well-defined structure. The pasture presented heterogeneity in the morphology of the canopy due to the spatial distribution of soil fertility, resulting in canopy and producing higher dry matter at the top and reaching the lower production on the shoulder of topossequence. The sward has heterogeneity in morphology depending on the spatial distribution of soil fertility and the effective depth, resulting in the creation of two management areas: 1 → topo and pediment, and 2 → shoulder and half hillside.

Fiorelli J.,University of Sao Paulo | Da Fonseca R.,São Paulo State University | Morceli J.A.B.,Zootecnista | Dias A.A.,University of Sao Paulo
Engenharia Agricola | Year: 2010

This paper presents a different roof tiles influence study on the thermal comfort for broiler housings. The research was conducted at UNESP's Experimental Campus at Dracena, state of São Paulo, Brazil. Four prototypes in real scale were built, each with an area of 28 m 2. The prototype was covered with roof tiles made of recycled long-life packing material, ceramic tiles, ceramic tiles painted with white coating, and fiber/cement tiles. Temperatures inside the structures were recorded in the winter 2007 over a 90-day period. The results obtained indicated that recycled tile thermal behavior was similar to ceramic tiles. However for the winter period all the prototypes had presented comfort thermal index not in the broilers thermo neutral zone.

Furtado D.A.,Federal University of Campina Grande | Rocha H.P.,Zootecnista | Nascimento J.W.B.,Federal University of Campina Grande | Silva J.H.V.,Federal University of Paraiba
Engenharia Agricola | Year: 2010

The aim of this research is to determine the thermal comfort index, the concentration of gases inside the poultry houses and their influence on the performance of commercial broiler chickens, located in the Paraibás semiarid region, in summer conditions. It was used two poultry houses, one of them covered with a ceramic roof and the other covered with fibrous cement roof. There was no significant difference (p> 0.05) between ambient temperature (AT), relative humidity (RH) and temperature of the drinking water (Twater) and the chicken litter between the warehouses, but the values average of AT (from 08:00 a.m. to 5:00 p.m.) and RH (from10:00 a.m. to 4:00 p.m.) can be considered outside the boundary of the comfort zone recommended for chicken broilers, but also the Twater was above the recommended limits, during the day, which is 24° C. None of the chicken broilers showed concentrations of gases that offer an unhealthy ambient to the birds and to the workers. Although the Twater and the index of thermal comfort are elevate during the hottest days, the production indices were within the established for the Brazilian poultry industry.

Leao A.G.,São Paulo State University | Silva Sobrinho A.G.,São Paulo State University | Moreno G.M.B.,São Paulo State University | de Souza H.B.A.,São Paulo State University | And 3 more authors.
Revista Brasileira de Zootecnia | Year: 2012

With the objective of evaluating physic-chemical and sensorial characteristics of meat from lambs finished in feedlot with diets containing sugar cane or corn silage at two roughage:concentrate ratios, 60:40 or 40:60, 32 non-castrated Ile de France lambs, with 15 kg body weight were used. Animals were confined in individual stalls and were slaughtered at 32 kg body weight. Studied diets and muscles did not affect pH at 45 minutes (6.56) and 24 hours (5.62) after slaughter, water holding capacity (58.38%) or cooking losses (34.04%). Meat color and subcutaneous fat color did not differ between diets, but meat color varied for the muscles longissimus lumborum and triceps brachii. Shear force (1.85 kgf/cm2) was not affected by diets, but it was different between muscles, with values 1.41 and 2.28 kgf/cm2 for the longissimus lumborum and triceps brachii, respectively. At sensorial analysis of ovine loin and shoulder, the lambs fed sugar cane and higher quantity of concentrate had higher scores for flavor (8.07 and 8.26), texture (8.53 and 8.53), preference (8.20 and 8.46) and acceptance (8.33 and 8.26), respectively. In the diet for feedlot lambs, sugar cane maintained the physic-chemical quality of the meat, so it can be used in this production phase. When associated to higher quantity of concentrate in the diet, sugar cane improves the sensorial quality of lamb meat. © 2012 Sociedade Brasileira de Zootecnia.

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