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Santa Bárbara d'Oeste, Brazil

Dos Santos Almeida R.,Brazilian Office of Pharmacovigilance of Radiopharmaceuticals | Mamede M.,Federal University of Minas Gerais | Santos-Oliveira R.,Brazilian Office of Pharmacovigilance of Radiopharmaceuticals | Santos-Oliveira R.,Zona Oeste Estadual University
Adverse Drug Reaction Bulletin | Year: 2013

Radiopharmaceuticals are used commonly and their pharmacokinetics can be altered by drug interactions with other prescribed medicines and interactions with the equipment used to administer them. No pharmacovigilance studies have been performed on their use in Brazil. In order to evaluate the quality, efficacy and security of the radiopharmaceuticals in use in Brazil, a study was conducted involving the main users of radiopharmaceuticals, that is hospitals. A total of 12 hospitals agreed to participate in this study. During the study period (2 years), a questionnaire was administered. Over 400 patients were interviewed and their comments were recorded. Only one of a subset of 55 patients with cancer who received Tc-MDP suffered an adverse reaction. The number of patients in this study was small; however, these results are quite similar to those found in European studies. © 2013 Lippincott Williams & Wilkins.

Cerqueira-Coutinho C.,Federal University of Rio de Janeiro | Santos-Oliveira R.,Zona Oeste Estadual University | dos Santos E.,Federal University of Rio de Janeiro | Mansur C.R.,Federal University of Rio de Janeiro
Engineering in Life Sciences | Year: 2015

Sunscreens are destined to topical application and should protect skin against ultraviolet radiation; furthermore, they are toxic substances and should not reach the bloodstream, so they must be retained in the skin. In the present work, an oil-in-water photoprotective and antioxidant nanoemulsion (NE) containing chitosan was developed. Preliminary studies were performed aiming to choose the surfactant to be used in this NE; stability of the formulas was determined by dynamic light scattering after their preparation and after 7 days. A blend of surfactants, polyoxyethylene sorbitan monooleate, and sorbitan monooleate was selected for the preparation of the NE, as well as the following organic sunscreens: benzophenone-3, diethylamino hydroxybenzoyl hexylbenzoate, octocrylene and octylmethoxycinnamate, and also pomegranate antioxidant extract and chitosan. The antioxidant extract with the highest antioxidant activity was chosen based on a screening of plant extracts by DPPH• (2,2-diphenyl-1-picrylhydrazyl) assay. Photostability of the sunscreens and in vitro efficacy and safety of the formulations were also evaluated. Results showed that the developed photoprotective and antioxidant NE containing chitosan was stable for at least 6 months, photostable when irradiated in a solar simulator, and effective. Additionally, chitosan acted by promoting retention of the formulation in epidermis, thus increasing formulation safety. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Szwed M.,University of Lodz | Santos-Oliveira R.,Zona Oeste Estadual University
Journal of Nanoscience and Nanotechnology | Year: 2016

In the present study we report the interactions of four types of different nanoparticles with normal peripheral blood mononuclear cells. To our research we chose four types of nanoparticles which possess therapeutic properties (Trastuzumab, ethylene-diamine-tetra-methylene-phosphonic for breast and bone cancers treatment, respectively) or can be used as the ingredients of sun-protected films (nanoemulsions with or without chitosan). By carrying out XTT survival assay we observed that both types of tested nanoemulsions suppressed the proliferation of normal lymphocytes. However, the survival of peripheral blood mononuclear cells after incubation neither with Trastuzumab nor with ethylene-diamine-tetra-methylene-phosphonic nanoparticles decreased below 80%. If the investigated nanoparticles were analyzed for their effectiveness to the induction of programmed cell death, we proved that only nanoemulsions with or without chitosan provoked an increase of the fraction of apoptotic cells. Moreover we noticed the characteristic, typical for apoptosis changes of cells morphology, which appeared in lymphocytes after all tested nanoparticles treatment. Interestingly, representative for necrosis swollen, enlarged cells were observed after nanoemulsions treatment. Copyright © 2016 American Scientific Publishers. All rights reserved.

Pinto S.R.,Zona Oeste Estadual University | Sarcinelle M.A.,Laboratory of Nanoradiopharmaceuticals | de Souza Albernaz M.,Laboratory of Nanoradiopharmaceuticals | da Silva F.M.R.,Laboratory of Nanoradiopharmaceuticals | And 5 more authors.
Nuclear Medicine and Biology | Year: 2014

The use of in vivo assay to determine the biodistribution and subsequent inter-comparison with human parameters has been used since the dawn of science. The use of this type of test admits the metabolic equity among animals for inter-comparison. Thus, the use of Wistar rats in particular is quite frequent. Regarding routes of administration, there are three ways to test priority: jugular vein, intraocular (eye plexus) and caudal; there is a consensus that these three pathways behave in the same way, or at least very similar. Biodistribution studies of drugs, especially radiopharmaceuticals, have been using randomly any of these pathways believed to be effective in their likeness without worrying about your real analytic equity. In this study, we performed in vivo assay in 8 Wistar rats using 99mTc -labeled Herceptin to review the route of administration on the biodistribution result. Thus, four mice were injected via the intraocular (eye plexus), and four were injected via tail (caudal plexus). The results were quite disparate and call the attention of the scientific community to reassess the protocols for animal experiments, in order to have uniformity and fairness between the data and may represent a test for human inter-comparison of more reliable and trustworthy way. © 2014 Elsevier Inc.

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