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Zhuzhou, China

Xu M.,Central South University | Zhang L.,Central South University | Zhao L.,Suzhou University | Gao S.,Central South University | And 3 more authors.
Rheumatology International

The purpose of this study is to observe the differences of osteopontin (OPN) phosphorylation in osteoarthritis (OA) cartilage and normal cartilage, and evaluate the possible correlations between the OPN phosphorylation and MMP-13 expression. Degenerative cartilage (n = 29) and normal cartilage (n = 10) were identified by hematoxylin-eosin, safranin-O staining and modified Mankin score. The phosphorylation level of OPN in OA cartilage and normal cartilage was detected by immunoprecipitation. Chondrocytes were treated with phospho-OPN, OPN or buffer. Quantitative reverse transcription polymerase chain reaction (qPCR) and ELISA were used to assess the expression of MMP-13 in different treatments. The OD values of phosphorylation of OPN in normal cartilage and OA cartilage were 137.89 ± 10.59 and 153.52 ± 8.80, respectively, (P = 0.000). Chondrocytes treated with OPN showed a higher MMP-13 expression at gene and protein level compared with control group. Chondrocytes treated with phospho-OPN showed the highest MMP-13 expression in gene and protein. In conclusion, our results revealed a higher phosphorylation level of OPN in OA cartilage than in normal cartilage. We found OPN leads to elevated expression of MMP-13 (both at gene level and protein level), and phospho-OPN had a more obvious upregulation effect on MMP-13 expression than nonphospho-OPN. Further studies are needed to reveal the mechanism of OPN phosphorylation on cartilage degeneration. © 2012 Springer-Verlag Berlin Heidelberg. Source

Xiao W.-X.,Zhuzhou No.1 Hospital | Zhang Y.-L.,Zhuzhou No.1 Hospital
International Eye Science

AIM: To discuss the effect and necessity of pars plana vitrectomy(PPV) for the treatment of vitreous hemorrhage complicated retinal hole or detachment. METHODS: Totally 28 cases(28 eyes) with vitreous hemorrhage complicated retinal hole or detachment were treated by PPV. The change of visual acuity was observed pre-and postoperatively and the relationship between vitreous hemorrhage and detachment was analyzed. RESULTS: In 28 cases with vitreous hemorrhage caused by various reasons, 7 cases occurred no retinal detachment from preoperative B ultrasound, 3 cases found retinal hole in intraoperation, 4 cases accompanied peri-hole shallow detachment. Postoperative visual acuity of 28 cases (including postoperative follow-up best-corrected visual acuity) improved in various degree, better than counting finger or before eye in 27 cases(96%), ≥0.05 in 20 cases(71%), ≥0.3 in 5 cases(18%).There was statistical significant difference between pre-and postoperation(P<0.05). CONCLUSION: It is safe and effective for vitreous hemorrhage complicated retinal hole or detachment treated by PPV, and can find retinal hole and shallow detachment as soon as possible so as to avoid further enlargement of retinal detachment. Source

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