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Li X.,CAS Dalian Institute of Chemical Physics | Qu Y.,CAS Dalian Institute of Chemical Physics | Zhang P.,Zhuanghe Entry Exit Inspection and Quarantine Bureau | Zhang J.,Agilent Technologies | And 3 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2011

Nine representative fish species from Bohai Bay were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and lab-on-a-chip technology. The nine fish species were Pseudosciaena polyactis, Lateolabrax japonicus, Scomberomorus niphonius, Pneumatophorus japonicus, Pseudopleuronectes yokohamae, Collichthys lucidus, Sebastes schlegeli, Cleisthenes herzensteini and Saurida elongata. The 464 bp fragment of mitochondrial cytochrome b gene was amplified and further digested by Dde I, Hae III and Nla III. The digested DNA fragments were analyzed by microfluidic capillary electrophoresis. The nine fish species were fully discriminated. The results demonstrated that the proposed method is efficient; precise and fast in fish species identification. Source


Xiaolin Z.,Zhuanghe Entry Exit Inspection and Quarantine Bureau | Xi C.,Zhuanghe Entry Exit Inspection and Quarantine Bureau | Ying L.,Zhuanghe Entry Exit Inspection and Quarantine Bureau | Daliang H.,Zhuanghe Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Chemistry Bulletin / Huaxue Tongbao | Year: 2012

A gas chromatography method was developed for simultaneous determination of 36 kinds organic phosphorus residues in vegetable. The target compounds were extracted using accelerated solvent extraction (ASE) and purified by gel permeation chromatography (GPC). According to the requirements of NO SANCO-10684 2009 [1] ,The recoveries of organic phosphorus residues in vegetable were in the range from 73.2% to 111.3% with the relative standard deviations less than 15% at the three spiked levers, i.e. 10,15, and 20 μg/kg. The method possesses the advantages of low background and high sensitivities. It can be applied to the food safety analysis of the organic phosphorus in vegetable. Source

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