Zhoushan Hospital of Zhejiang Province

Zhoushan, China

Zhoushan Hospital of Zhejiang Province

Zhoushan, China
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PubMed | Zhoushan Hospital of Zhejiang Province, Zhejiang Ocean University and Yanbian University
Type: Journal Article | Journal: Chemical biology & drug design | Year: 2016

Herein, a series of 3-phenyliminoindolin-2-one derivatives were designed, synthesized, and screened for their antidepressant and anticonvulsant activities. The IR spectra of the compounds afforded NH stretching (3340-3346 cm(-1)) bands and C=O stretching (1731-1746 cm(-1)). In the (1)H-NMR spectra of the compounds, N-H protons of indoline ring were observed at 10.65-10.89 ppm generally as broad bands, and (13)C-NMR spectra of the compounds C=O were seen at 161.72-169.27 ppm. Interestingly, compounds 3o, 3p and 3r significantly shortened immobility time in the The forced swimming test (FST) and The tail suspension test (TST) at 50 mg/kg dose levels. In addition, compound 3r exhibited higher levels of efficacy than the reference standard fluoxetine but had no effect on locomotor activity in the open-field test. Compound 3r significantly increased serotonin and norepinephrine and the metabolite 5-hydroxyindoleacetic acid in mouse brain, suggesting that the effects of compound 3r may be mediated through these neurotransmitters. In the seizure screen, 15 compounds showed some degree against PTZ-induced seizure at a dose of 100 mg/kg, and the tested compounds did not show any neurotoxicity at a dose of 300 mg/kg in the rotarod test.


Zhu W.,Chinese Academy of Sciences | Liu X.,Chinese Academy of Sciences | He J.,Chinese Academy of Sciences | Chen D.,Chinese Academy of Sciences | And 2 more authors.
BMC Cancer | Year: 2011

Background: Lung cancer is the leading cause of cancer-related deaths worldwide. Early detection is considered critical for lung cancer treatment. MicroRNAs (miRNAs) have shown promise as diagnostic and prognostic indicators. This study was to identify specific miRNAs with diagnostic and prognostic value for patients with lung cancer, and to explore the correlation between expression profiles of miRNAs and patient survival.Methods: Gene expression of members of the miR-183 family (miR-96, miR-182, and miR-183) were examined in 70 paired samples from lung cancer patients (primary cancer and non-cancerous tissues and sera), as well as 44 serum samples from normal volunteers and lung cancer cell lines by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR). The correlation between the expression of miRNAs in tissues, sera, and patient overall survival were also examined by log-rank and Cox regression analysis.Results: Expression levels of members of the miR-183 family in lung cancer tumor and sera were higher than that of their normal counterparts. The miR-96 expression in tumors was positively associated with its expression in sera. Log-rank and Cox regression analyses demonstrated that high expression of tumor and serum miRNAs of the miR-183 family were associated with overall poor survival in patients with lung cancer.Conclusions: Our results suggest that the expressions of miR-96, miR-182, and miR-183 in tumor and sera may be considered potential novel biomarkers for the diagnosis and prognosis of lung cancer. © 2011 Zhu et al; licensee BioMed Central Ltd.


Wang Z.,Zhoushan Hospital of Zhejiang Province | Sun H.,Tongji University | Yakisich J.S.,Karolinska University Hospital
Anti-Cancer Agents in Medicinal Chemistry | Year: 2014

Treatment of brain tumors with chemotherapy is limited mostly because of delivery impediments related to the blood-brain barrier (BBB). For gliomas, the most common and aggressive primary brain tumor, treatment includes surgery, radiotherapy, and chemotherapy usually administered orally or intravenously. These routes do not deliver effective concentrations. To complicate matters, chemotherapy is usually a long treatment. Therefore, transient disruption of the BBB is likely insufficient to deliver effective intratumoral concentrations of anticancer drugs. This review briefly updates current strategies for overcoming the BBB with emphasis on their limitations and challenges intrinsic to the biology of cancer cells. © 2014 Bentham Science Publishers.


Sun H.,Tongji University | Wang Z.,Zhoushan Hospital of Zhejiang Province | Yakisich J.S.,Karolinska University Hospital
Anti-Cancer Agents in Medicinal Chemistry | Year: 2013

The phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway is a key regulator of authophagy. Natural products show anticancer activity and often induce apoptosis or autophagy. The crosstalk between these two types of cell death makes autophagy an interesting target since drugs targeting this process not only can induce cell death by inducing autophagy but can also sensitize cells to apoptosis. Autophagy is also a protective mechanism associated with increased resistance to chemotherapy. In this review, we discuss natural products known to induce autophagy cell death in cancer cells via the PI3K/Akt/mTOR pathway. © 2013 Bentham Science Publishers.


Le H.,Zhoushan Hospital of Zhejiang Province | Zeng F.,Chinese Academy of Sciences | Xu L.,Chinese Academy of Sciences | Liu X.,Chinese Academy of Sciences | Huang Y.,Chinese Academy of Sciences
Molecular Medicine Reports | Year: 2013

Cancer stem cells (CSCs) are a small population of undifferentiated cancer cells within tumors, which contribute to tumorigenicity and relapse. In the current study, CD133 (also termed prominin-1), a CSC marker, was investigated to determine its involvement in predicting carcinogenesis and prognosis in patients with non-small cell lung carcinoma (NSCLC). CD133-positive lung cancer cells were isolated to analyze self-renewal, differentiation and tumorigenic abilities in vitro and in vivo. Quantitative polymerase chain reaction was used to detect the expression of CD133 and three other CSC-associated markers, octamer-binding transcription factor 4 (OCT4A), Nanog homeobox (NANOG) and multidrug resistance protein 1 (MDR1), in primary NSCLC and adjacent non-cancer tissues. A series of statistical methods were used to analyze the correlation between mRNA expression levels, clinicopathological features and patient survival. The results showed that CD133-positive NSCLC cells demonstrated clonogenic, tumorigenic and drug-resistance properties compared with their CD133-negative counterparts or parental cells. In addition, compared with the adjacent normal lung tissue, the levels of CSC-associated biomarkers CD133, OCT4A, NANOG and MDR1 were significantly increased in NSCLC tissue. Elevated expression of CD133 was associated with stage, tumor size and differentiation of NSCLC; however, the cox hazard regression analysis showed no significant association between CD133 expression and overall patient survival. The present study supports the hypothesis that the stem cell population can be enriched in cells expressing the CD133 cell surface marker and that highly expressed CD133 is involved in the occurrence of NSCLC. However, CD133 may not be considered as an independent factor in predicting the prognosis of patients with NSCLC. Further studies are required to investigate the association between CD133 expression and overall patient survival.


Yi J.-H.,Zhejiang University | Liu H.-B.,Zhoushan Hospital of Zhejiang Province | Zhang M.,Zhejiang University | Wu J.-S.,Zhejiang University | And 4 more authors.
Journal of Zhejiang University: Science B | Year: 2012

Objective: To evaluate the efficacy and safety of the treatment of traumatic hemothorax by closed pleural drainage using a central venous catheter (CVC), compared with using a conventional chest tube. Methods: A prospective controlled study with the Ethics Committee approval was undertaken. A total of 407 patients with traumatic hemothorax were involved and they were randomly assigned to undergo closed pleural drainage with CVCs (n=214) or conventional chest tubes (n=193). The Seldinger technique was used for drainage by CVC, and the conventional technique for drainage by chest tube. If the residual volume of the hemothorax was less than 200 ml after the daily volume of drainage decreased to below 100 ml for two consecutive days, the treatment was considered successful. The correlative data of efficacy and safety between the two groups were analyzed using t or chi-squared tests with SPSS 13.0.A P value of less than 0.05 was taken as indicating statistical significance. Results: Compared with the chest tube group, the operation time, fraction of analgesic treatment, time of surgical wound healing, and infection rate of surgical wounds were significantly decreased (P<0.05) in the CVC group. There were no significant differences between the two groups in the success rate of treatment and the incidence of serious complications (P>0.05), or in the mean catheter/tube indwelling time and mean medical costs of patients treated successfully (P>0.05). Conclusions: Management of medium or large traumatic hemothoraxes by closed thoracic drainage using CVC is minimally invasive and as effective as using a conventional large-bore chest tube. Its complications can be prevented and it has the potential to replace the large-bore chest tube. © Zhejiang University and Springer-Verlag Berlin Heidelberg 2012.


Zhang Y.-K.,Zhoushan Hospital of Zhejiang Province | Zhu W.-Y.,Zhoushan Hospital of Zhejiang Province | He J.-Y.,Zhoushan Hospital of Zhejiang Province | Chen D.-D.,Zhoushan Hospital of Zhejiang Province | And 3 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2012

Purpose: We investigated whether miRNA expression profiles can distinguish and predict outcome of non-small-cell lung carcinoma (NSCLC) patients with different his-tological subtypes. Methods: High-throughput microarray was used to measure miRNA expression levels in six NSCLC samples. Subsequently, quantitative reverse transcription polymer-ase chain reaction (qRT-PCR) was used to verify findings in an independent set of 54 squamous-cell lung carcinomas (SCC), 51 lung adenocarcinomas (AD), and paired adjacent non-neoplastic lung tissue. Results: We showed that, compared to adjacent non-neo-plastic lung tissues, the expressions of miR-125a-5p and let-7e were decreased in AD and SCC samples, while increased expressions of miR-93, miR-205, and miR-221 were observed in SCC samples. In addition, miR-205 expression was significantly higher in SCC patients with lymph node metastasis. Lower let-7e expression was associated with lymph node metastasis, >3 cm tumor size, and differentiation of the NSCLC AD subtype. High levels of miR-100 expression also correlated with the AD subtype in current smokers. Moreover, induction of miR-93 and miR-205 expressions and reduction of let-7e were strongly associated with shorter overall survival in SCC patients, whereas AD patient survival was only associated with reduced let-7e. Conclusions: We identified differential expression profiles of miRNAs in AD and SCC. More importantly, in addition to morphology and immunocytochemistry approaches, we report that miR-93, miR-205, miR-221, and let-7e may represent novel biomarkers for differential diagnosis and prognosis of certain NSCLC subtypes or be new targets of histology-specific treatments. Furthermore, our results suggest a strong correlation between high expression of miR-100 and AD patients with history of heavy smoking. © Springer-Verlag 2012.


Zhu W.,Zhoushan Hospital of Zhejiang Province
Wei sheng yan jiu = Journal of hygiene research | Year: 2012

To investigate the nutritional status of iodine and the prevalence of thyroid carcinoma of residents in Zhoushan Archipelago, and to explore the related factors influencing the prevalence of thyroid carcinoma. The residents in Zhoushan Archipelago were selected by cluster random sampling. The subjects were surveyed by questionnaire, their thyroids were examined by B-ultrasonography, the thyroid function and the levels of urine iodine were analyzed. Pathological diagnosis was conducted to the patients suspected of thyroid carcinoma. The median levels of urine iodine in urban residents, famers, salt-makers, fishermen from Zhoushan and buddhists from Putuoshan were 320.7, 188.9, 122.2, 193.6 and 271.7 microg/L respectively, while the prevalence rates of thyroid carcinoma were 215/100 000, 398/100 000 0, 407/100 000, 829/100 000,340/100 000, respectively; The age or a higher level of hTSH of a person were risk factors for suffering from thyroid carcinoma. The iodine intake of residents in ZhouShan Archipelago is adequate and the prevalence rate of thyroid carcinoma is high. Serum hTSH could be used as a predictor for the risk of thyroid carcinoma.


Liu X.-G.,Chinese Academy of Sciences | Zhu W.-Y.,Chinese Academy of Sciences | Huang Y.-Y.,Chinese Academy of Sciences | Ma L.-N.,Chinese Academy of Sciences | And 6 more authors.
Medical Oncology | Year: 2012

Serum microRNAs have been identified as potential cancer biomarkers. However, the detailed mechanism by which expression of microRNAs contributes to the development and diagnosis of NSCLC remains unknown. This study was to identify specific miRNAs for diagnosing or predicting the prognosis of NSCLC patients and their correlation between miRNA expression in tissues and serums. Six matched cancer and noncancerous tissues from NSCLC patients were analyzed by miRNA microarray. Among these, three miRNAs (miR-21, miR-141, and miR-200c) were examined in 70 NSCLC paired samples (cancer, normal tissue, and serum) and 44 serum samples of normal volunteers by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Consisting with the microarray results, the expression levels of miR-21, miR-141, and miR-200c in NSCLC were higher than those in normal tissues. While the level of serum miR-21 was increased in cancer patients as compared with that in normal counterpart, expression of miR-141 and miR-200c showed lower levels in serums from cancer patients. Overexpression of serum miR-21 was strongly associated with lymph node metastasis and advanced clinical stage of NSCLC. Finally, log-rank and Cox regression tests demonstrated that high expressions of tumor miR 21 and miR-200c or serum miR-21 were associated with a poor survival in NSCLC patients. Our results suggest that tumor miR-21, miR-141, miR-200c, and serum miR-21 may be potential novel biomarkers for the diagnosis of NSCLC. In addition, this study, for the first time, identifies a significant role of the tumor miR-200c played in predicting prognosis in patients with NSCLC. © Springer Science+Business Media, LLC 2011.


Huang Y.,Chinese Academy of Sciences | Zeng F.,Chinese Academy of Sciences | Xu L.,Chinese Academy of Sciences | Zhou J.,Chinese Academy of Sciences | And 2 more authors.
Oncology Research | Year: 2013

Lung cancer is a lethal solid tumor with poor prognosis because of its high metastasis and resistance to current therapies. Recently, cancer stem cells (CSCs) were suggested to be major contributors to tumorigenicity and cancer relapse. However, therapeutic targets for lung cancer-related CSCs remain undetermined. The objective of the current study was to investigate whether cinnamic acid (CINN) exerts an antitumor activity against sphere-derived lung CSCs. In this study, CSCs were isolated from the non-small cell lung cancer cell line H1299 as tumor spheres under CSC-selective conditions, and found to have increased tumorigenicity, chemoresistance, and higher expression of both embryonic stem cell-related and drug resistance-related genes compared with parental cells. These observations are consistent with the notion that CSCs are tumorigenic, display the ability to self-renew, and generate differentiated progeny that constitute the majority of cells in tumors. Treatment of sphere-derived stem cells with CINN could diminish their CSC-like abilities by decreasing their proliferation and invasive abilities and facilitating their differentiation into CD133-negative cells. Furthermore, CINN treatment increased the sensitivity of CSCs to chemotherapeutic drugs through apoptosis. Of note, xenotransplantation experiments revealed that CINN combined with cisplatin had a synergistic effect in inhibiting the tumorigenicity of CSCs. In summary, our study clearly revealed the presence of a population of sphere-forming cells with stem-like properties among H1299 cells and CINN can attenuate CSC properties of this stem-like cell population. The potential of CINN should be verified further in future studies of anti-CSC therapy. Copyright © 2013 Cognizant Comm. Corp.

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