Zhongshan Hospital of Hubei Province

Wuhan, China

Zhongshan Hospital of Hubei Province

Wuhan, China

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Tong Z.,Wuhan University | Liu W.,Wuhan University | Yan H.,Zhongshan Hospital of Hubei Province | Dong C.,Tsinghua University
Immunology | Year: 2015

Interleukin-17 (IL-17) is a cytokine with critical functions in multiple autoimmune diseases. However, its roles in type I diabetes and the underlying mechanisms remain to be fully elucidated. In the current study, we investigated the impact of IL-17 deficiency on streptozotocin (STZ) -induced diabetes. Il-17-/- mice exhibited attenuated hyperglycaemia and insulitis after STZ treatment compared with control mice. The Il-17-/- mice had fewer CD8+ cells infiltrating the pancreas than wild-type controls after STZ injection. Wild-type mice showed increased percentage and number of splenic CD8+ cells and decreased Gr1+ CD11b+ myeloid-derived suppressor cells (MDSC) after STZ treatment, but Il-17-/- mice maintained the percentages and numbers of splenic CD8+ cells and MDSC, suggesting that IL-17 is implicated in STZ-induced cellular immune responses in the spleen. We further purified the MDSC from spleens of STZ-treated mice. Il-17-/- MDSC showed increased ability to suppress CD8+ cell proliferation in vitro compared with wild-type MDSC. Transfer of MDSC to diabetic mice showed that MDSC from Il-17-/- mice could ameliorate hyperglycaemia. Moreover, recipients with MDSC from Il-17-/- mice had a decreased percentage of CD8+ cell in the spleen compared with recipients with MDSC from wild-type mice. These data suggest that IL-17 is required in splenic MDSC function after STZ delivery. In summary, our study has revealed a pathogenic role of IL-17 in an STZ-induced diabetes model with important implications for our understanding of IL-17 function in autoimmune diseases. © 2015 John Wiley & Sons Ltd.


PubMed | The First Hospital of Wuhan and Zhongshan Hospital of Hubei Province
Type: Journal Article | Journal: Molecular medicine reports | Year: 2015

The aim of the present study was to investigate the chemical composition of the essential oil of Artemisia vestita and to determine the antibacterial activity of the essential oil and its two major components, grandisol and 1,8cineole, against certain respiratory infectioncausing bacterial strains, in vitro and in vivo. The chemical composition of the essential oil was analyzed using gas chromatographymass spectrometry. A microwell dilution method was used to determine the minimum inhibition concentration (MIC) values of the essential oil and its major constituents. A model of Streptococcus pyogenes infection in mice was used to determine its in vivo activities. Lung and blood samples were obtained to assess bacterial cell counts. Toxicity evaluation of the essential oil and its components was completed by performing biochemical analysis of the serum, particularly monitoring aspartate transaminase, alanine transaminase, urea and creatinine. The essential oil exhibited potent antibacterial activity, whereas the two major constituents were less potent. The essential oil exhibited MIC values between 20 and 80 g/ml, while the values of the two constituents were between 130 and 200 g/ml. Scanning electron microscopy results demonstrated that the essential oil inhibited biofilm formation and altered its architecture. Survival curves indicated that the essential oil led to a reduction in the viability of different bacteria. The essential oil also induced significant leakage of potassium ions from S. pyogenes. The essential oil (100 g/mouse) and grandisol (135 g/mouse) significantly reduced the number of viable bacterial cells in the lungs (P<0.01). However, intake of 100 g/mouse of essential oil or grandisol 135 g/mouse once or twice each day for 9 days did not produce any toxic effects in the mice. In conclusion, the in vitro and in vivo results suggested that the essential oil of A. vestita and one of its major constituents, grandisol, can significantly inhibit the growth of different bacterial strains.


Zhao Z.,Tianjin Medical University | Li Y.,The Peoples Hospital of Liao Cheng | Liu S.,Zhongshan Hospital of Hubei Province | Fu W.,Tianjin Medical University
Tumor Biology | Year: 2014

Gastric cancer (GC) is a highly malignant cancer with increasing incidence and mortality worldwide. Serum Helicobacter pylori CagA antibody has been widely reported to play an important role in diagnosing GC. However, published data on this subject are inconclusive. Therefore, we performed a meta-analysis to evaluate the sensitivity and specificity of serum H. pylori CagA antibody in the diagnosis of GC. We conducted a comprehensive search to identify eligible related studies, in which the pooled sensitivity, specificity, diagnostic odds ratio (DOR), and summary receiver operating characteristic (SROC) curves could be determined. A total of 12 studies including 1,524 cases and 3,324 controls who fulfilled all of the inclusion criteria were included for analysis. The summary estimates for serum H. pylori CagA antibody in the diagnosis of GC in these studies were pooled sensitivity 0.71 (95 % CI 0.69-0.73), specificity 0.40 (95 % CI 0.39-0.42), DOR 2.11 (95 % CI 1.55-2.8), and the area under the curve was 0.636. Our meta-analysis showed that serum H. pylori CagA antibody should not be used for detecting GC. © 2014 International Society of Oncology and BioMarkers (ISOBM).


Zhu P.,Huazhong University of Science and Technology | Yan F.,Zhongshan Hospital of Hubei Province | Yang Z.,Huazhong University of Science and Technology | Meng L.,Huazhong University of Science and Technology | Ao Q.,Huazhong University of Science and Technology
Oncology Letters | Year: 2013

Metanephric adenoma (MA) and Wilms' tumor (WT) are two distinct types of renal tumors. Composite MA and WT of the kidney are extremely rare. Here, a rare case of composite MA and WT of the kidney in a 36-year-old male is described. MA and WT each have their own histopathological features, respectively, and they focally share morphological similarities, which can be a diagnostic challenge. Immunohistochemistry is useful in the differential diagnosis of MA and WT. The histopathological features and differential diagnosis of the composite tumor are emphasized here to promote a better and broader understanding of this less understood subject.


Yang C.,Zhongshan Hospital of Hubei Province | Hu D.-H.,Zhongshan Hospital of Hubei Province | Feng Y.,First Hospital of Wuhan
Molecular Medicine Reports | Year: 2015

The aim of the present study was to investigate the chemical composition of the essential oil of Artemisia vestita and to determine the antibacterial activity of the essential oil and its two major components, grandisol and 1,8-cineole, against certain respiratory infection-causing bacterial strains, in vitro and in vivo. The chemical composition of the essential oil was analyzed using gas chromatography-mass spectrometry. A micro-well dilution method was used to determine the minimum inhibition concentration (MIC) values of the essential oil and its major constituents. A model of Streptococcus pyogenes infection in mice was used to determine its in vivo activities. Lung and blood samples were obtained to assess bacterial cell counts. Toxicity evaluation of the essential oil and its components was completed by performing biochemical analysis of the serum, particularly monitoring aspartate transaminase, alanine transaminase, urea and creatinine. The essential oil exhibited potent antibacterial activity, whereas the two major constituents were less potent. The essential oil exhibited MIC values between 20 and 80 μg/ml, while the values of the two constituents were between 130 and 200 μg/ml. Scanning electron microscopy results demonstrated that the essential oil inhibited biofilm formation and altered its architecture. Survival curves indicated that the essential oil led to a reduction in the viability of different bacteria. The essential oil also induced significant leakage of potassium ions from S. pyogenes. The essential oil (100 μg/mouse) and grandisol (135 μg/mouse) significantly reduced the number of viable bacterial cells in the lungs (P<0.01). However, intake of 100 μg/mouse of essential oil or grandisol 135 μg/mouse once or twice each day for 9 days did not produce any toxic effects in the mice. In conclusion, the in vitro and in vivo results suggested that the essential oil of A. vestita and one of its major constituents, grandisol, can significantly inhibit the growth of different bacterial strains.


Su T.,Huazhong University of Science and Technology | Yan F.,Zhongshan Hospital of Hubei Province | Zhu P.,Huazhong University of Science and Technology
Diagnostic Pathology | Year: 2014

Background: Metanephric neoplasms comprised a spectrum of kidney tumors containing renal epithelial or stromal cells or both, including metanephric adenoma, metanephric stromal tumor, and metanephric adenofibroma. The majority of cases were benign; only one case of " metanephric adenosarcoma" had been reported in the English literature.History: We present the case of a 69-year-old man who developed a neoplasm composed of renal epithelial component identical to metanephric adenoma combined with malignant spindle-cell stroma. The epithelial component was positive for CD57, AE1/AE3, but negative for WT-1, CD56, SYN, and CgA; whereas the sarcomatous component was negative for epithelial markers, SMA, Caldesmon, MyoD1, Myogenin, and S-100; and positive for vimentin, CD10, and WT1 focally. No specific sarcoma differentiation was apparent in the stroma by immunohistochemistry, and no SYT-SS18 rearrangement or BRAF mutation was detected by molecular analysis.A diagnosis of metanephric adenosarcoma was made because of the morphological features and immunohitochemistry and molecular pathology analysis.Clinical significance: We believe that metanephric adenosarcoma should be in the expanded spectrum of metanephric neoplasia as a malignant stromal variant.Conclusions: We report a rare case of metanephric adenosarcoma with immunohistochemistry and molecular analysis and emphasize the histopathologic features and differential diagnosis of the rare lesion to promote a better and broader understanding of this less understood subject.Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_179. © 2014 Su et al.; licensee BioMed Central Ltd.


Zhu P.,Huazhong University of Science and Technology | Yan F.,Zhongshan Hospital of Hubei Province | Ao Q.,Huazhong University of Science and Technology
International Journal of Clinical and Experimental Pathology | Year: 2014

Ectopic micronodular thymoma (MNT) is a rare tumor. We described a 76-year-old woman, who was referred to our institutional for a mass in the left cervical region. The Magnetic Resonance Imaging (MRI) scan showed a 3.7 cm × 1.7 cm × 2.0 cm mass. The neoplasm was composed of epithelial tumor cells arranged in a micronodular growth pattern set in a stroma showing lymphoid hyperplasia with germinal centers. Immunohistochemical studies showed that the neoplastic epithelial cells were reactive for AE1/AE3, CK5/6, P63, and the lymphoid component to be of mixed B- and immature T-cell lineage. Langerhans cells were confirmed within epithelial nodules for the first time with langerin, S-100, CD1a expression. We report a case of cervical ectopic MNT to emphasize the langerhans cells proliferation and the histopathologic features and differential diagnosis of the rare lesion to promote a better and broader understanding of this less understood subject.


Guo J.,Zhongshan Hospital of Hubei Province
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2012

To compare and analyse the efficiency of the low-temperature plasma radiofrequency and the traditional method in tonsillectomy. Sixty-four patients with chronic tonsillitis were randomly divided into group A (39 cases) and group B (25 cases). Patients in the group A were treated with traditional method tonsillectomy while patients in the group B were treated with low-temperature plasma radio frequency tonsillectomy. Analysed differences in operation time, blood loss, and postoperative pain as well as the incidence of postoperative between two groups. There were significant differences in operative time, blood loss, and postoperative pain (P < 0.05) between two groups, but there was no obvious difference in the incidence of postoperative bleeding (P > 0.05). Low-temperature plasma radiofrequency tonsillectomy is a safer method and has many superiorities including shorter operative time, less blood loss, quicker recovery of postoperative pain compared with traditional tonsillectomy.


Huang D.-H.,Huazhong University of Science and Technology | Zhang S.-W.,Zhongshan Hospital of Hubei Province | Zhao H.,Huazhong University of Science and Technology | Zhang L.,Huazhong University of Science and Technology
Asian Journal of Andrology | Year: 2011

C-type natriuretic peptide (CNP) is a 22-amino acid peptide and act as a local paracrine or autocrine regulator. There is growing evidence that CNP is involved in male reproductive processes. To investigate the role of CNP during spermatogenesis, we measured the mRNA expression of CNP and its specific membrane-bound natriuretic peptide receptor-B (NPR-B) using real-time RT-PCR in the testes of normal rats on different postnatal days. After that spermatogenesis dysfunction model induced by ornidazole was established with the aim to study the correlation of CNP with spermatogenic dysfunction. Then, Sertoli cells from 18-to 22-day-old healthy male rats were cultured in the presence of different CNP concentrations (1×10-6, 1×10-7 and 1×10-8 moll-1), and the mRNA expression levels of androgen-binding protein, inhibin B and transferrin were examined at 0min, 30min, 1h, 2h, 4h, 8h, 12h, 24h and 48h. During the postnatal development of rat testes, the highest mRNA expression levels of CNP and NPR-B were found at postnatal D 0, and the levels then declined gradually, with a second CNP peak at postnatal D 35. In the ornidazole-induced infertile rat testes, CNP gene expression was lower than in the uninduced rats (P0.05), while NPR-B gene expression was greater (P0.05). In cultured Sertoli cells, supplementation with CNP stimulated the gene expression of androgen-binding protein/inhibin B/transferrin, particularly at 12h, and 1×10-7 mol l-1 CNP had the highest upregulation effect. The gene expression levels of CNP/NPR-B in rat testes at different postnatal stages and in infertile rat testes indicated that CNP may participate in the physiology and/or pathology related to spermatogenesis. Moreover, CNP regulated endocrine function in Sertoli cells. Taken together, these results showed that CNP is closely tied to spermatogenesis. © 2011 AJA, SIMM & SJTU. All rights reserved.


PubMed | Zhongshan Hospital of Hubei Province and Huazhong University of Science and Technology
Type: Journal Article | Journal: Oncology letters | Year: 2015

Reactive nodular fibrous pseudotumor (RNFP) is a tumor-like lesion that is characterized by reactive fibroblast/myofibroblast proliferation within collagenic hyalinized stroma, due to its association with injury or inflammation. The current study describes the case of a 60-year-old female with a history of abdominal surgery and abdominal pain. Upon laparoscopy, multiple nodules attached to the outer layer of the colon and mesentery were identified, and therefore, complete surgical excision was performed. Macroscopically, the nodules were well-circumscribed, firm, tan-white in color and ranged in size between 2.0-10.0 cm at the greatest dimension. Microscopically, the nodules were composed of spindle and stellate cells in a dense collagenic hyalinized background with sparse lymphocytic infiltration. Immunohistochemical analysis demonstrated positive staining for vimentin, smooth muscle actin and cluster of differentiation (CD) 117, and focally-positive keratin staining with AE1/AE3; however, no staining was observed for gastrointestinal stromal tumor 1, CD34, S-100, anaplastic lymphoma kinase or -catenin. Therefore, it was proposed that the lesion may be most accurately described as an RNFP. The current study reports a rare case of RNFP, emphasizing its histopathological features and differential diagnoses to promote an improved and broader understanding of this poorly understood condition.

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