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Zhongshan, China

Peng W.-B.,Southern Medical University | Liu C.-X.,Southern Medical University | Xie Q.,Zhongshan Boai Hospital | Zhu B.-L.,Zhongshan Guzhen Hospital | Lv T.-R.,Zhongshan Guzhen Hospital
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2013

Objective: To construct tissue engineered urethra with enriched rabbit hair follicle stem cells, and observe its effect in repair of urethral defect. Methods: Rabbit hair follicle cells were isolated and cultured by mechanical separation and enzyme digestion, and were in vitro cultured and proliferated to 3×106. Integrin-α6+/cd71- cells of <10 μm in diameter were sorted by flow cytometry, and the expression of K19, p63 and β1 integrin was detected by immunofluorescence method. Hair follicle stem cells were seeded on bladder acellular matrix scaffold, tissue engineered urethra was constructed, and the cell distribution on scaffold was observed by scanning electron microscope. Rabbit urethral defect of 2.5 cm was repaired with tissue engineered urethra, and the effect of repair was observed by urethrography and histological examination. Results: Immunofluorescence detection revealed that there was positive expression of K19, p63 and β1 integrin in hair follicle stem cells. Scanning electron microscope demonstrated that cells firmly attached to the scaffold and moderately stretched, and there was matrix secretion. Histological and immunohistochemical examinations indicated there was stratified epithelium-like structure, with positive staining of AE1/AE3 in hair follicle cells. Three months after repair of urethral defect by tissue engineered urethra, urethrography illustrated the urethra was smooth and unobstructed. Conclusion: Tissue engineered urethra constructed by enriched rabbit hair follicle stem cells can be used for repair of rabbit urethral defect. Source

Zhang Y.,Hong Kong Polytechnic University | Luximon A.,Hong Kong Polytechnic University | Ma X.,Hong Kong Polytechnic University | Guo X.,Zhongshan Boai Hospital | Zhang M.,Hong Kong Polytechnic University
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) | Year: 2011

Nowadys consumers are sophisticated and want better quality personalized products at lower prices. In order to satisfy consumers, footwear companies have to predict consumers' trends and produce huge varieties of designs using mass production technologies. This results in wastage and high inventory cost. Recently mass customization methodologies have been proposed. Mass customization enables the design and production of an almost custom made product at almost mass production costs. This can be achieved by reducing the wastage and inventory cost. The products are designed specifically for the consumer, eventually improving consumer satisfaction and the companies' market share. Many researchers have discussed the framework of mass customization; however, there is limited study on the system details, especially for footwear design. The main purpose of this research is to propose a methodology for the mass customization of footwear - both style and fit customization. © 2011 Springer-Verlag. Source

Wang G.L.,Zhongshan Boai Hospital | Lu J.R.,Jilin University
Science China Life Sciences | Year: 2010

This work reports the investigation of the effect of airway IL-4RA gene transfer by a recombinant retroviral vector on airway inflammation and airway responsiveness in asthmatic mice. The retrovirus-mediated delivery of IL-4RA to the airways of mice inhibited elevations of airway responsiveness and the development of allergic inflammation in asthmatic mice, and regulated the Th1/Th2 balance in OVA-sensitized and -challenged mouse models. This suggests that gene therapy is a therapeutic option for treating and controlling chronic airway inflammation and asthma symptoms. © 2010 Science China Press and Springer-Verlag Berlin Heidelberg. Source

Zeng Z.,Sun Yat Sen University | Zhu Z.,Sun Yat Sen University | Yang Y.,Zhongshan Peoples Hospital | Ruan W.,Zhongshan Peoples Hospital | And 13 more authors.
Journal of Gastroenterology and Hepatology (Australia) | Year: 2013

Background and Aims: The incidence of inflammatory bowel disease (IBD) is increasing in China with urbanization and socioeconomic development. There is however a lack of prospective, population-based epidemiology study on IBD in China. The aim of the study is to define the incidence and clinical characteristics of IBD in a developed region of Guangdong Province in China. Methods: A prospective, population-based incidence study was conducted from July 2011 to June 2012 in Zhongshan, Guangdong, China. All newly diagnosed IBD cases in Zhongshan were included. Results: In total, 48 new cases of IBD (17 Crohn's disease [CD]; 31 ulcerative colitis [UC]) were identified over a 1-year period from July 2011. Age-standardized incidence rates for IBD, UC, and CD were 3.14, 2.05, and 1.09 per 100000 persons, respectively. The median age of UC was 38, and that of CD was 25. Terminal ileum involvement only (L1), isolated colonic disease (L2), and ileocolonic disease (L3) were reported in 24%, 6%, and 71% of patients with CD, respectively. Twenty-four percent of patients had coexisting upper gastrointestinal disease (L4). Inflammatory (B1), stricturing (B2), and penetrating (B3) behavior were seen in 65%, 24%, and 12% of CD patients, respectively. Fifty-nine percent of CD and 26% of UC patients had extra-intestinal manifestations. Conclusions: This is the first prospective, population-based IBD epidemiological study in a developed region of China. The incidence of IBD is similar to that in Japan and Hong Kong but lower than that in South Korea and Western countries. © 2013 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd. Source

Song Y.-Z.,Jinan University | Zhang Z.-H.,Jinan University | Lin W.-X.,Jinan University | Zhao X.-J.,Jinan University | And 13 more authors.
PLoS ONE | Year: 2013

Background:The human SLC25A13 gene encodes citrin, the liver-type mitochondrial aspartate/glutamate carrier isoform 2 (AGC2), and SLC25A13 mutations cause citrin deficiency (CD), a disease entity that encompasses different age-dependant clinical phenotypes such as Adult-onset Citrullinemia Type II (CTLN2) and Neonatal Intrahepatic Cholestasis caused by Citrin Deficiency (NICCD). The analyses of SLC25A13 gene and its protein/mRNA products remain reliable tools for the definitive diagnoses of CD patients, and so far, the SLC25A13 mutation spectrum in Chinese CD patients has not been well-characterized yet.Methods and Results:By means of direct DNA sequencing, cDNA cloning and SNP analyses, 16 novel pathogenic mutations, including 9 missense, 4 nonsense, 1 splice-site, 1 deletion and 1 large transposal insertion IVS4ins6kb (GenBank accession number KF425758), were identified in CTLN2 or NICCD patients from China, Japan and Malaysia, respectively, making the SLC25A13 variations worldwide reach the total number of 81. A large NICCD cohort of 116 Chinese cases was also established, and the 4 high-frequency mutations contributed a much larger proportion of the mutated alleles in the patients from south China than in those from the north (χ2 = 14.93, P<0.01), with the latitude of 30°N as the geographic dividing line in mainland China.Conclusions:This paper further enriched the SLC25A13 variation spectrum worldwide, and formed a substantial contribution to the in-depth understanding of the genotypic feature of Chinese CD patients. © 2013 Song et al. Source

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