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Hu L.,Zhengzhou Tobacco Institute of CNTC | Taujale R.,Northern Illinois University | Liu F.,Zhengzhou Tobacco Institute of CNTC | Song J.,Zhengzhou Tobacco Institute of CNTC | And 4 more authors.
Journal of Biotechnology | Year: 2016

Talaromyces verruculosus has long been known to be a supreme cellulase and antimicrobial extrolite producer. Here we report the draft genome of T. verruculosus strain TS63-9, which has a very high pectinase activity. The assembled draft genome is 37.35 Mbp with 11,447 predicted protein-coding genes, among which 532 are annotated to be carbohydrate active enzymes (CAZymes) and 225 are polysaccharide-degrading enzymes. © 2015 Elsevier B.V.


PubMed | Zhengzhou Tobacco Institute of CNTC and Northern Illinois University
Type: | Journal: Journal of biotechnology | Year: 2016

Talaromyces verruculosus has long been known to be a supreme cellulase and antimicrobial extrolite producer. Here we report the draft genome of T. verruculosus strain TS63-9, which has a very high pectinase activity. The assembled draft genome is 37.35 Mbp with 11,447 predicted protein-coding genes, among which 532 are annotated to be carbohydrate active enzymes (CAZymes) and 225 are polysaccharide-degrading enzymes.


Ma G.,Beijing University of Chemical Technology | Liu Y.,Beijing University of Chemical Technology | Liu Y.,Changzhou University | Peng C.,Beijing University of Chemical Technology | And 5 more authors.
Carbohydrate Polymers | Year: 2011

A highly porous chitosan nanofibers were obtained by electrospinning chitosan/polyethylene oxide (PEO) blend solutions and then removing PEO with water. The porous morphology of the nanofibers was observed by scanning electron microscopy. The porous nanofibers were soaked in 0.1 wt% paclitaxel solution to load the cancer drug. Then a polyanion nature macromolecular hyaluronic acid (HA) was encapsulated on the chitosan polycation porous nanofibers by immersing the fibers into a 4 wt% HA aqueous solution. Differential scanning calorimetry (DSC) and Fourier transform infrared (FT-IR) were used to investigate the polymer formation and the interaction among two nature macromolecular and the cancer drugs. The paclitaxel release profiles of the encapsulated fibers in PBS were analyzed by UV-vis spectrophotometer. In vitro DU145 prostate cancer cells activities of the nanofibers were examined by MTT. Cell culture results showed that the PTX-loaded nanofibers mats were good in prohibiting the cell attachment and proliferation. These results strongly suggested that the chitosan/hyaluronic acid fibers had an effect of controlled release of paclitaxel and were suitable for postoperative chemotherapy of prostate cancers. © 2011 Elsevier Ltd All rights reserved.


Hu S.-W.,Nanjing University | Xu B.-Y.,Nanjing University | Qiao S.,Nanjing University | Zhao G.,Zhengzhou Tobacco Institute of CNTC | And 3 more authors.
Talanta | Year: 2016

In this work, we report a novel microfluidic gas collecting platform aiming at simultaneous sample extraction and multiplex mass spectrometry (MS) analysis. An alveolar-mimicking elastic polydimethylsiloxane (PDMS) structures was designed to move dynamically driven by external pressure. The movement was well tuned both by its amplitude and rhythm following the natural process of human respiration. By integrating the alveolar units into arrays and assembling them to gas channels, a cyclic contraction/expansion system for gas inhale and exhale was successfully constructed. Upon equipping this system with a droplet array on the alveolar array surface, we were able to get information of inhaled smoke in a new strategy. Here, with cigarette smoke as an example, analysis of accumulation for target molecules during passive smoking is taken. Relationships between the breathing times, distances away from smokers and inhaled content of nicotine are clarified. Further, by applying different types of extraction solvent droplets on different locations of the droplet array, simultaneous extraction of nicotine, formaldehyde and caproic acid in sidestream smoke (SS) are realized. Since the extract droplets are spatially separated, they can be directly analyzed by MS which is fast and can rid us of all complex sample separation and purification steps. Combining all these merits, this small, cheap and portable platform might find wide application in inhaled air pollutant analysis both in and outdoors. © 2015 Elsevier B.V. All rights reserved.


PubMed | Nanjing University and Zhengzhou Tobacco Institute of CNTC
Type: | Journal: Talanta | Year: 2016

In this work, we report a novel microfluidic gas collecting platform aiming at simultaneous sample extraction and multiplex mass spectrometry (MS) analysis. An alveolar-mimicking elastic polydimethylsiloxane (PDMS) structures was designed to move dynamically driven by external pressure. The movement was well tuned both by its amplitude and rhythm following the natural process of human respiration. By integrating the alveolar units into arrays and assembling them to gas channels, a cyclic contraction/expansion system for gas inhale and exhale was successfully constructed. Upon equipping this system with a droplet array on the alveolar array surface, we were able to get information of inhaled smoke in a new strategy. Here, with cigarette smoke as an example, analysis of accumulation for target molecules during passive smoking is taken. Relationships between the breathing times, distances away from smokers and inhaled content of nicotine are clarified. Further, by applying different types of extraction solvent droplets on different locations of the droplet array, simultaneous extraction of nicotine, formaldehyde and caproic acid in sidestream smoke (SS) are realized. Since the extract droplets are spatially separated, they can be directly analyzed by MS which is fast and can rid us of all complex sample separation and purification steps. Combining all these merits, this small, cheap and portable platform might find wide application in inhaled air pollutant analysis both in and outdoors.

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