Zhang X.,Zhengzhou University of Light Industry |
Wang H.,Zhengzhou University of Light Industry |
Zhang L.,Orthopedics Hospital of Zhengzhou |
Zhao Q.,Zhengzhou University
Optik | Year: 2014
This article shows that we obtained blood plasma of healthy persons and patients with leukemia, got absorption spectrum of them by spectral photometer. After analyzing these absorption spectra by the origin software, we found absorbance (Abs) of the malignant plasma were more than Abs of the healthy plasma at 414 nm, and we also found significant difference between the malignant plasma and the healthy plasma when Abs of some characteristic peaks at 414 nm, 279 nm were rationed or fitted. It is important for doctors to diagnose patients with leukemia quickly. © 2014 Elsevier GmbH. All rights reserved.
Guo X.-W.,Orthopedics Hospital of Zhengzhou |
Guan C.-X.,Zhengzhou University
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Hematopoietic stem cell transplantation has therapeutic effects on many diseases, but its application has some limitations, such as cell harvesting and age-limited number of cells. OBJECTIVE: To investigate the application value of bone marrow mesenchymal stem cells in hematopoietic stem cell transplantation for sensitized and non-sensitized BALB/c mice. METHODS: Bone marrow cells derived from BALB/c mice were isolated and cultured in vitro to harvest mesenchymal stem cells using adherent method. The cell surface markers were detected by flow cytometry. A murine model of sensitization was established by transfusion of allogeneic spleen cells. Mesenchymal stem cells labeled with green fluorescent dye were transplanted into non-sensitized and sensitized recipient mice, and the homing of mesenchymal stem cells in vivo was monitored at different time points post transplantation. Additionally, under irradiation pretreatment, sensitized BALB/c mice under irradiation were subjected to combined transplantation of allogeneic bone marrow cells and syngeneic mesenchymal stem cells. Survival rate of BALB/c mice was monitored daily. RESULTS AND CONCLUSION: At 48 hours after transplantation, mesenchymal stem cells in sensitized and non-sensitized recipients were homing to the spleen and bone marrow, respectively. In the experiment of hematopoietic stem cell transplantation, the sensitized recipients died at 12_15 days after combined transplantation, with a median of 14 days; however, the sensitized recipients only undergoing allogeneic bone marrow cell transplantation had a survival median of 13 days. These findings indicate that the transplanted mesenchymal stem cells in sensitized recipients are mainly homing to the spleen and bone marrow, but the combination transplantation cannot enhance the transplantation of allogeneic hematopoietic stem/progenitor cells in sensitized recipients. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.