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Wu L.,Zhengzhou University | Dong B.,No. 1 Peoples Hospital of Zhengzhou | Zhang F.,Zhengzhou Central Hospital | Li Y.,No. 1 Peoples Hospital of Zhengzhou | Liu L.,Zhengzhou University
Dermatologica Sinica | Year: 2016

Objective Our aim was to explore the engendering mechanism and gene targets for melanoma. Methods The microarray data of GSE46517 were downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) between primary melanoma samples and normal controls were analyzed using the GEO2R online tool. The screened DEGs were mapped to a protein-protein interaction network based on the Search Tool for the Retrieval of Interacting Genes database. The functions and pathways involved with DEGs were analyzed using the Database for Annotation Visualization and Integrated Discovery software) online tools. Then, the DEGs were further annotated via the TRANSFAC, Tumor-Suppressor Gene, and Tumor-Associated Gene databases. Results A total of 1095 DEGs including 511 upregulated genes and 584 down-regulated ones were screened out. The nodes of CCL5, ISG15, CDKN2A, EGFR, and ERBB2 showed a high connectivity degree in protein-protein interaction networks and were mainly enriched in Biological Process GO terms such as the regulation of catalytic activity and cell adhesion, as well as the pathways of cytochrome P450. The DEGs were classified into 31 transcription factors and 43 downregulated tumor associated genes. Conclusion Catalytic activity, cell adhesion, and the cytochrome P450 associated pathways are dysregulated in the melanoma formation. The significant nodes such as ISG15, IRF4, ERBB2 and EGFP may be potential targets for primary melanoma treatment. © 2015, Taiwanese Dermatological Association. Published by Elsevier Taiwan LLC.


Hao Y.,Zhengzhou Central Hospital | Fan T.,Zhengzhou University | Nan K.,Xian Jiaotong University
Computational and Mathematical Methods in Medicine | Year: 2015

Aims. To optimize and verify the regulatory pathway of p42.3 in the pathogenesis of gastric carcinoma (GC) by intelligent algorithm. Methods. Bioinformatics methods were used to analyze the features of structural domain in p42.3 protein. Proteins with the same domains and similar functions to p42.3 were screened out for reference. The possible regulatory pathway of p42.3 was established by integrating the acting pathways of these proteins. Then, the similarity between the reference proteins and p42.3 protein was figured out by multiparameter weighted summation method. The calculation result was taken as the prior probability of the initial node in Bayesian network. Besides, the probability of occurrence in different pathways was calculated by conditional probability formula, and the one with the maximum probability was regarded as the most possible pathway of p42.3. Finally, molecular biological experiments were conducted to prove it. Results. In Bayesian network of p42.3, probability of the acting pathway "S100A11→RAGE→P38→MAPK→Microtubule-associated protein→Spindle protein→Centromere protein→Cell proliferation" was the biggest, and it was also validated by biological experiments. Conclusions. The possibly important role of p42.3 in the occurrence of gastric carcinoma was verified by theoretical analysis and preliminary test, helping in studying the relationship between p42.3 and gastric carcinoma. © 2015 Yibin Hao et al.


Zhou F.,Chongqing Medical University | Zhou F.,U.S. Center for Disease Control and Prevention | Zhang L.,U.S. Center for Disease Control and Prevention | Gao L.,Chinese Academy of Sciences | And 8 more authors.
PLoS ONE | Year: 2014

Objective: To determine the impact factors of latent tuberculosis infection (LTBI) and the knowledge of TB prevention and treatment policy among health care workers (HCWs) in different types of hospitals and explore the strategies for improving TB prevention and control in medical institutions in China. Methods: A cross-sectional study was carried out to evaluate the risk of TB infection and personnel occupational protection among HCWs who directly engage in medical duties in one of two public hospitals. Each potential participant completed a structured questionnaire and performed a tuberculin skin test (TST). Factors associated with LTBI were identified by logistic regression analysis. Results: Seven hundred twelve HCWs completed questionnaires and 74.3% (n = 529) took the TST or had previous positive results. The TST-positive prevalence was 58.0% (n = 127) in the infectious disease hospital and 33.9% (n = 105) in the non-TB hospital. The duration of employment in the healthcare profession (6-10 years vs. ≤5 years [OR = 1.89; 95% CI = 1.10, 3.25] and.10 vs. ≤5[OR = 1.80; 95% CI = 1.20, 2.68]), type of hospital (OR = 2.40; 95% CI = 1.59, 3.62), and ever-employment in a HIV clinic or ward (OR = 1.87; 95% CI = 1.08, 3.26)were significantly associated with LTBI. The main reasons for an unwillingness to accept TST were previous positive TST results (70.2%) and concerns about skin reaction (31.9%). Conclusion: A high prevalence of TB infections was observed among HCWs working in high-risk settings and with long professional experiences in Henan Province in China. Comprehensive guidelines should be developed for different types of medical institutions to reduce TB transmission and ensure the health of HCWs. © 2014 Zhou et al.


Liu Y.Y.,Zhengzhou Central Hospital
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences | Year: 2012

To investigate synergistically killing effect of histone deacetylase (HDAC) inhibitor suberoylanilide hydroxamic acid (SAHA) combined with imatinib on human chronic myeloid leukemia (CML) cell line. K562 cells were co-treated with SAHA and imatinib. Cell growth was measured by MTT assay. Apoptosis was determined using Hoechst staining apoptosis detection kit and flow cytometric analysis. Activation of Caspase pathway, expression of Bcr-Abl and its downstream target genes, and expression of anti-apoptotic proteins were detected by Western blot. SAHA synergized the cytotoxicity of imatinib against leukemia K562 cells, concomitantly with increased apoptosis and enhanced activation of Caspase-3, -8 and PRAP. The combination therapy resulted in significantly lower levels of Bcr-Abl,phosphorylated Bcr-Abl compared to treatment with either SAHA or imatinib alone. Furthermore,the co-treatment resulted in down-regulation of anti-apoptotic protein Mcl-1 expression. Also,marked down-regulated expression of JAK2,STAT5,and phosphorylated STAT5 was detected in the combination therapy. Combining HDAC inhibitor SAHA with imatinib can kill CML cells synergistically by inhibiting cell growth and inducing apoptosis, which is associated with activation of Caspase pathway and regulation of anti-apoptotic proteins.


Xu X.F.,Zhengzhou TCM Hospital | Hu J.P.,Zhengzhou TCM Hospital | Cheng X.,Zhengzhou TCM Hospital | Yu G.J.,CAS Zhengzhou Research Institute | And 4 more authors.
Journal of biological regulators and homeostatic agents | Year: 2016

This study explores the effects of sodium ferrous chlorophyll treatment on the anemia of maintenance hemodialysis (MHD) patients, as well as the relevant biochemical parameters. We selected 72 patients who had received regular MHD treatment two or three times a week for more than 3 months in the Hospital of Traditional Chinese Medicine of Zhengzhou City of Henan Province from March 2014 to March 2016. They were equally divided into a treatment group and a control group. Haemoglobin (HB) and hematocrit (HCT) of the treatment group increased significantly after treatment (p < 0.01), but less in the control group (p < 0.05); Also serum ferritin (SF) and transferrin saturation (TAST) of the treatment group increased significantly after treatment (p < 0.01); SF of the control group also increased significantly (p < 0.01) and TAST of the control group increased (p < 0.05) but less than in the treatment group. No obvious changes of serum creatinine (SCR), blood urea nitrogen (BUN), C-reactive protein (CRP) and superoxide dismutase (SOD) were found in either groups after treatment (p>0.05). Albumin (ALB) dosage of the treatment group increased after treatment (p < 0.05) while hemopoietin (EPO) decreased significantly (p < 0.01). ALB and EPO of the control group had no obvious changes after treatment (p>0.05). ALB level of the treatment group increased more significantly than in the control group (p < 0.05), while EPO dosage decreased more significantly than in the control group (p <0.05). Therefore, the combination of conventional western medicine and sodium ferrous chlorophyll can effectively improve anemia conditions of MHD patients and their quality of life.

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