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Zhang M.,Zhejiong Academy of Science and Technology for Inspection and Quarantine | Zhang X.,Zhejiong Academy of Science and Technology for Inspection and Quarantine | Chen X.,Zhejiong Academy of Science and Technology for Inspection and Quarantine | Wu S.,Zhejiong Academy of Science and Technology for Inspection and Quarantine | Chen W.,Zhejiong Academy of Science and Technology for Inspection and Quarantine
Journal of Chinese Institute of Food Science and Technology | Year: 2014

The enzyme-linked probe hybridization chip is a method based on ligase-hybridizing probe chip technology, with the principle of using the thio-primers for protection, and using lambda DNA exonuclease to cut multiple PCR products into single chains. 5'-end probe was fixed in the amino-modified chip, and hybridized with the single-chain product, adding the fluorescent-modified probe for the enzyme-linked reactions with the anterior probe, in order to achieve highly specific, parallel and high-throughput detection. According to the specificity and sensitivity experiments, the enzyme-linked probe hybridization chip could effectively eliminate the false positive signals and the sensitivity limit is 0.1%, would be applied to inspection, quarantine and food safety. ©, 2014, Chinese Institute of Food Science and Technology. All right reserved. Source

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