Zhejiang UniversityZhejiang

Medicine, China

Zhejiang UniversityZhejiang

Medicine, China
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Bai L.,Chongqing Medical University | Chen Y.,Chongqing Medical University | Bai Y.,Chongqing Medical University | Zhou J.,Chongqing Medical University | And 2 more authors.
Biomaterials | Year: 2017

Tuberculosis caused by Mycobacterium tuberculosis (MTB) is still a major threat to global public health. However, the existing methods for MTB detection are usually complicated and time consuming with unsatisfactory sensitivity and specificity. In this work, a relatively simple and ultrasensitive electrochemical aptasensor based on novel signal generation and amplification was constructed for the determination of MTB antigen MPT64. The coil-like fullerene (C60)-doped polyaniline (C60-PAn) nanohybrids with large surface area, abundant active groups and excellent electric performance were synthesized and used both as new redox nanoprobe and catalyst for the generation and amplification of electrochemical signal for the first time. Then gold nanoparticles decorated C60-PAn nanocomposites (GNPs-C60-PAn) were labeled with signal aptamer to form the tracer label. After the sandwich reaction of target MPT64 antigen between capture aptamer and the tracer label, a distinguishing detection signal of C60-PAn would be observed. Moreover, the detection signal could be enormously enhanced towards the efficient electrocatalytic oxidation of ascorbic acid based on C60-PAn, resulting in further improvement of the sensitivity. With the excellent redox and electrocatalytic activity of C60-PAn, a wide detection linear range from 0.02 to 1000 pg/mL was obtained with a detection limit of 20 fg/mL for MPT64. The proposed aptasensor showed high selectivity to target antigen compared with possible interfering substances. More importantly, it also exhibited excellent specificity and sensitivity for MPT64 detection in serum samples of tuberculosis patients, which provided a rapid and efficient detection method for MTB infection. © 2017 Elsevier Ltd


Hui E.C.M.,Hong Kong Polytechnic University | Dong Z.,Hong Kong Polytechnic University | Dong Z.,Zhejiang UniversityZhejiang | Jia S.,Zhejiang UniversityZhejiang | Lam C.H.L.,Hong Kong Polytechnic University
Habitat International | Year: 2017

Many urbanized housing markets have been overheating over the last two decades, particularly in China. However, little is known about how sentiment affects housing returns, during the dynamic process of urbanization. With this in mind, this study aims to investigate the dynamic effect of sentiment on housing returns in one of the most important urbanized cities in China, i.e. Shanghai. The study creates the buyer-seller sentiment and developer sentiment indexes using principal components analysis, followed by implementing a lag-property return model and VAR model. Evidence suggest that overall the impact of buyer-seller sentiment on housing returns is negative, while that of developer sentiment is positive. Yet interestingly, the influence of sentiment marked a noticeable change within the period under study. Before 2009, both types of sentiment had a positive effect on housing returns in the short run. After that, a higher developer sentiment drove up returns, but a higher buyer-seller sentiment had a low return. This study offers meaningful implications for policy makers in cooling down not only the housing scene of the urbanized city, but also sheds light on other urbanized housing markets globally. More importantly, it contributes to a sustainable urbanization and economic development, while providing home buyers and developers with practical suggestions. © 2017


Zu L.,Zhejiang UniversityZhejiang | Zhu W.,Zhejiang UniversityZhejiang | Dong H.,Zhejiang UniversityZhejiang | Ke Y.,Zhejiang UniversityZhejiang
Composite Structures | Year: 2017

The non-geodesics using variable slippage coefficients along the winding trajectories are gaining increasing attention for composite pressure vessels. The aim of this paper is to evaluate the influence of several slippage coefficient distributions on fiber trajectories and the resulting mass of toroidal pressure vessels. The distributions of slippage coefficients are represented in terms of three functions satisfying C1 continuity. The governing equations for designing non-geodesics on a toroid are formulated using the given functions of slippage coefficients. For each function, the initial fiber angle and the shell thickness are considered as the design variables, and the minimum mass is taken as the objective. With the aid of the classical lamination theory and the SQP algorithm, the optimal fiber trajectories are respectively determined for the given three slippage coefficient distributions, and the corresponding masses of the toroids are then obtained and compared to each other. The results indicate that toroids produced using variable slippage coefficients show improved performance than toroids using constant slippage coefficients; this is mainly due to the maximum utilization of the laminate strength. It is also revealed that the weight efficiency of toroidal vessels can be significantly improved using the quadratic distribution function of fiber slippage coefficients. © 2017 Elsevier Ltd


Jian L.,The Second Affiliated Hospital of Zhengzhou UniversityHenan | Jian D.,Zhejiang UniversityZhejiang | Chen Q.,Zhejiang UniversityZhejiang | Zhang L.,Zhejiang UniversityZhejiang
Journal of Atherosclerosis and Thrombosis | Year: 2016

Long noncoding RNAs (lncRNAs) were a group of non-protein-coding RNAs >200 nucleotides and participated in biological processes and pathophysiological conditions in vivo or in vitro. Recently, more and more lncRNAs interfering with the progress of atherosclerosis were identified and characterized in the atherogenic cells such as vascular smooth muscle cells (VSMCs), endothelial cells (ECs), and monocytes/macrophages showing that lncRNAs play an important role in the occurrence of atherosclerosis. In this review, we summarized and highlighted the lncRNAs that play a role in the process of atherosclerosis. This study may provide helpful insights regarding further study of lncRNAs associated with atherosclerosis. © 2016, Journal of Atherosclerosis and Thrombosis. All rights reserved.


Liang H.,Zhejiang University | Xu Z.,Zhejiang University | Zheng Z.,Hengdian Hospital of Zhejiang ProvinceZhejiang | Lou H.,Zhejiang UniversityZhejiang | Yue W.,Tianjin Huanhu Hospital
Orphanet Journal of Rare Diseases | Year: 2015

Background: Reversible cerebral vasoconstriction syndrome (RCVS) is an infrequent disease characterized by severe headaches with or without focal neurological deficits or seizures and a reversible vasoconstriction of cerebral arteries. The Orpha number for RCVS is ORPHA284388. However, RCVS triggered by blood transfusion is rare. Here we provided the clinical, neuroimaging and outcome data of patients diagnosed with RCVS resulting from red blood cells transfusion. Methods: We retrospectively identified 7 patients presenting with RCVS after red blood cells transfusion from January 2010 to May 2014. The information on clinical features, neuroimaging and outcome were collected and analyzed. Results: All 7 patients were Chinese women, with a mean age of 42 years (38-46). All the patients had severe anemia (Hb level<6 g/dl) caused by primary menorrhagia due to uterine myoma (n=5) or end-stage renal disease (n=2) and severe anemia persisted for a average period of 4 months (2-6). Each patient received packed red blood cells transfusion (average: 1580 ml) over a period of 2-5 days. Blood transfusion increased the hemoglobin level by at least 4.5 g/dL from baseline. The neurological symptoms appeared a mean of 6.3 days (2-13) after the last blood transfusion. Headache was the most frequent symptom and seizure, transient or persistent neurological disorders were observed. Neuroimaging showed cortical subarachnoid hemorrhage (n=2), focal intracerebral hemorrhage (n=2), localized brain edema (n=3), cerebral infarction (n=1), and posterior reversible encephalopathy syndrome (n=2). Cerebral vasoconstrictions were demonstrated by magnetic resonance angiography or cerebral angiography. Arterial constriction reversed in all patients within 1 to 3 months of follow-up after disease onset and no relapse was observed up to a mean of 17.1±4.8 months of follow-up. Conclusions: RCVS is a rare complication as a result of blood transfusion in patients with chronic severe anemia and should be considered in patients who show severe headache or neurologic deficits after transfusion. © 2015 Liang et al.; licensee BioMed Central.


PubMed | Zhejiang UniversityZhejiang, Sir Run Run Shaw Hospital Hangzhou and Zhejiang University
Type: Journal Article | Journal: American journal of translational research | Year: 2017

The study was designed to investigate the oxidative stress levels of endothelial progenitor cells (EPCs) in stable coronary artery disease (CAD) and to explore the underlying mechanisms of NADPH oxidase activation and subsequent EPCs dysfunction.EPCs were isolated from patients with stable CAD (n=50) and matched healthy volunteers (n=50). NADPH oxidase activation was detected by measuring the expression of each subunit using western blotting and qPCR analyses and the membrane translocation of p47Oxidative stress level was increased and the in vivo angiogenesis capacity was impaired in EPCs obtained from CAD subjects with the activation of NADPH oxidase. P47NADPH oxidase activation via p47


Waygood E.O.D.,Laval University | Sun Y.,Zhejiang UniversityZhejiang | Letarte L.,Laval University
International Journal of Environmental Research and Public Health | Year: 2015

Active travel can contribute to physical activity achieved over a day. Previous studies have examined active travel associated with trips in various western countries, but few studies have examined this question for the Asian context. Japan has high levels of cycling, walking and public transport, similar to The Netherlands. Most studies have focused either on children or on adults separately, however, having children in a household will change the travel needs and wants of that household. Thus, here a household lifecycle stage approach is applied. Further, unlike many previous studies, the active travel related to public transport is included. Lastly, further to examining whether the built environment has an influence on the accumulation of active travel minutes, a binary logistic regression examines the built environment’s influence on the World Health Organization’s recommendations of physical activity. The findings suggest that there is a clear distinction between the urbanized centers and the surrounding towns and unurbanized areas. Further, active travel related to public transport trips is larger than pure walking trips. Females and children are more likely to achieve the WHO recommendations. Finally, car ownership is a strong negative influence. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


Liu K.,New York University | Sun Z.,Zhejiang UniversityZhejiang | Wei T.,Zhejiang UniversityZhejiang
Clinical Medicine Insights: Cardiology | Year: 2015

Although most patients with Takotsubo cardiomyopathy (TTC) have benign clinical course and prognosis, TTC can induce acute heart failure and hemodynamic instability. TTC mimics the clinical features of acute anterior wall myocardial infarction (AMI). Bedside clinicians often have a diagnostic dilemma when cardiac catheterization and angiography are either contraindicated or can cause potential adverse consequences. Misdiagnosing TTC as AMI will lead to initiation of harmful pharmacological or device-based treatment, which worsens hemodynamic compromise. Therefore, under-standing and interpreting the unique pathophysiological and hemodynamic features of TTC in a better manner becomes crucial to guide effective clinical management of acute heart failure/cardiogenic shock during TTC. We review recent advances in echocardiographic diagnosis of TTC and its role in guid-ing bedside management of acute heart failure and cardiogenic shock, with specific focus on the interpretation of discrepant, but reciprocally dependent, left and right ventricular hemodynamics during acute stages of TTC. © the authors, publisher and licensee Libertas Academica Limited.


Zhang H.,Zhejiang UniversityZhejiang | Pei Y.,Zhejiang UniversityZhejiang
International Journal of Environmental Research and Public Health | Year: 2016

Quantitative prediction of construction noise is crucial to evaluate construction plans to help make decisions to address noise levels. Considering limitations of existing methods for measuring or predicting the construction noise and particularly the equivalent continuous noise level over a period of time, this paper presents a discrete-event simulation method for predicting the construction noise in terms of equivalent continuous level. The noise-calculating models regarding synchronization, propagation and equivalent continuous level are presented. The simulation framework for modeling the noise-affected factors and calculating the equivalent continuous noise by incorporating the noise-calculating models into simulation strategy is proposed. An application study is presented to demonstrate and justify the proposed simulation method in predicting the equivalent continuous noise during construction. The study contributes to provision of a simulation methodology to quantitatively predict the equivalent continuous noise of construction by considering the relevant uncertainties, dynamics and interactions. © 2016 by the authors; licensee MDPI, Basel, Switzerland.


Liang D.,Hokkaido University | Kaneko S.,Hokkaido University | Hashimoto M.,Chukyo University | Iwata K.,Japan National Institute of Advanced Industrial Science and Technology | Zhao X.,Zhejiang UniversityZhejiang
Pattern Recognition | Year: 2015

An illumination-invariant background model for detecting objects in dynamic scenes is proposed. It is robust in the cases of sudden illumination fluctuation as well as burst motion. Unlike the previous works, it uses the co-occurrence differential increments of multiple pixel pairs to distinguish objects from a non-stationary background. We use a two-stage training framework to model the background. First, joint histograms of co-occurrence probability are employed to screen supporting pixels with high normalized correlation coefficient values; then, K-means clustering-based spatial sampling optimizes the spatial distribution of the supporting pixels; finally the background model maintains a sensitive criterion with few parameters to detect foreground elements. Experiments using several challenging datasets (PETS-2001, AIST-INDOOR, Wallflower and a real surveillance application) prove the robust and competitive performance of object detection in various indoor and outdoor environments. © 2014 Elsevier Ltd. All rights reserved.

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