Yu M.-M.,Zhejiang Agriculture And forestry University |
Wu L.-Z.,Zhejiang Agriculture And forestry University |
Si J.-Z.,Zhejiang Agriculture And forestry University |
Uu J.-Z.,Zhejiang Agriculture And forestry University |
And 4 more authors.
Zhongguo Zhongyao Zazhi | Year: 2016
To reveal the relationship between endophytic fungi and the functional components, saccharides and flavonoids in the mycelia or fermented liquor of 21 endophytic fungi in D. officinale were detected using HPLC and UV spectrophotometer. The results showed tliat the ethyl acetate extracts from 21 fungal strains all contain flavonoids. According to the chromatographic retention time of HPLC and UV spectra characteristics of flavonoids, strain D()49 was found produce naringenin, strains D023, D081 and D083 were found produce rutin. The water-soluble extracts from 21 strains all had polysaccharides. However, there was difference in the composition of monosaccharides derived from polysaccharides among different strains. According to the composition of monosaccharides and the peak area ratio of mannose and glucose, the fungal strains including D023, D026, D081 , D054, D055, D083 product polysaccharides associated with D. officinale were selected. In conclusion, based on the saccharides and flavonoids, the excellent endophytic fungal strains D023, D081 and D083 were selected, which could produce the same flavonoids and similar polysaccharides in I), officinale.