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Hangzhou, China

Li J.,Zhejiang Agriculture And forestry University | Wang Y.,Zhejiang Senhe Seed Co. | Hua G.-J.,New Dimension Systems Co. | Wen F.,CAS Institute of Botany
Nordic Journal of Botany

A new species of Gesneriaceae, Primulina xiziae Fang Wen, Yue Wang & G. J. Hua, from Zhejiang Province, China, is identified, illustrated and described. It resembles the widespread P. eburnea, and the stenochoric P. pseudoeburnea, but differs by the leaf blades being ovate-elliptic or ovate, chartaceous with ciliate margins, 8.5-13.8 mm long, slender peduncle, ovate, ca 1.2 × 1.0 cm large bracts that are generally withered but persistent before full-blooming stage, slender tubular-infundibuliform, ca 25.0 × 7.5 mm corolla tube, limb lobes with acute apices, 3 staminodes, and by forming a dormancy bud in winter. © 2012 The Authors. Source

Jiang Y.-S.,CAS Institute of Botany | Zhang Y.,Zhejiang Senhe Seed Co. | Wang Y.,Zhejiang Senhe Seed Co. | Wen F.,CAS Institute of Botany
Nordic Journal of Botany

Petrocodon multiflorus F. Wen & Y. S. Jiang sp. nov. (Gesneriaceae) is described and illustrated. It is most similar to P. dealbatus Hance, but can be distinguished by its leaf lamina texture, shape of leaf margins, and by the number of cymes borne by an individual plant and the number of flowers in each cyme. A key to the species of Petrocodon is provided. © 2011 The Authors. Source

Wen F.,CAS Institute of Botany | Xi S.-L.,CAS Institute of Botany | Wang Y.,Zhejiang Senhe Seed Co. | Xiang M.-S.,Zhejiang Senhe Seed Co. | Fu L.-F.,Guangxi Normal University
Annales Botanici Fennici

Primulina fengshanensis Fang Wen & Yue Wang, a new species of Gesneriaceae from Guangxi, is described and illustrated. The new species is compared with the similar P. linearifolia and P. longgangensis. © Finnish Zoological and Botanical Publishing Board 2012. Source

Chen W.,Zhejiang Agriculture And forestry University | Qin Q.,Zhejiang Agriculture And forestry University | Zhang C.,Zhejiang Agriculture And forestry University | Zheng Y.,Zhejiang Senhe Seed Co. | And 5 more authors.
Plant Cell Reports

Key message: DhEFL2, 3and4regulate the flowering ofDoritaenopsis. These genes could rescueelf4-1phenotype in Arabidopsis while its overexpression delayed flowering.Abstract: Phalaenopsis are popular floral plants, and studies on orchid flowering genes could help develop off-season cultivars. EARLY FLOWERING 4 (ELF4) of A. thaliana has been shown to be involved in photoperiod perception and circadian regulation. We isolated two members of the ELF4 family from Doritaenopsis hybrid (Doritaenopsis ‘Tinny Tender’ (Doritaenopsis Happy Smile × Happy Valentine)), namely, DhEFL2 and DhEFL3 (DhEFL4 has been previously cloned). Multiple alignment analysis of the deduced amino acid sequences of the three DhEFL homologs showed that DhEFL4 and DhEFL2 are similar with 72 % identical amino acids, whereas DhEFL3 is divergent with 72 % similarity with DhEFL2 and 68 % similarity with DhEFL4. DhEFL3 forms a separate phylogenetic subgroup and is far away from DhEFL2 and DhEFL4. The diurnal expression patterns of DhEFL2, 3, and 4 are similar in the long-day photoperiod conditions; however, in the short-day conditions, DhEFL3 is different from DhEFL2 and 4. For the DhEFL2, 3, and 4 genes, the strongest audience expression organs are the stem, petal and bud, respectively. The ectopic expression of DhEFL2, 3, or 4 in transgenic A. thaliana plants (Ws-2 ecotype) showed novel phenotypes by late flowering and more rosette leaves. The ectopic expression of DhEFL2, 3, or 4 could complement the elf4-1 flowering time and hypocotyl length defects in transgenic A. thaliana elf4-1 mutant plants. These results strongly suggest that DhEFL2, 3, and 4 may be involved in regulation of flower formation and floral induction in Doritaenopsis. © 2015 Springer-Verlag Berlin Heidelberg Source

Jia M.-X.,Beijing Forestry University | Xu J.,Beijing Forestry University | Ye X.-J.,Zhejiang Senhe Seed Co. | Liu Q.,Beijing Forestry University | And 2 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal

To achieve the mass-scale multiplication of Dendrobium nobile 'Senhe 2006', the optimal media for the induction, multiplication and differentiation of protocorm-like bodies (PLBs), strengthening and rooting were studied and the rapid propagation system by tissue culture was established. The results showed that 1/2MS+TDZ 0.5 mg·L-1+casein hydrolysate 2.0 g·L-1 was optimal for PLB induction. 1/2MS+casein hydrolysate 2.0 g·L-1 was the most suitable for PLB multiplication. 1/2MS+KT 1.0 mg·L-1+NAA 0.1 mg·L-1 was the most efficient for PLB differentiation. The optimum medium for strengthening and rooting was MS+IBA 0.5 mg·L-1 +NAA 0.1 mg·L-1+banana homogenate 100.0 g·L-1. Source

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