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Mo F.,Zhejiang University | Chi C.,Zhejiang University | Guo M.,Chinese Research Academy of Environmental Sciences | Chu X.,Zhejiang Provincial Tongde Hospital | And 3 more authors.
Journal of Hazardous Materials | Year: 2014

Concentrations and risk of monoaromatic hydrocarbons (MAHC), formaldehyde (HCHO), and polycyclic aromatic hydrocarbons (PAHs) in two moxibustion rooms were determined. The mean concentrations of MAHC, HCHO and PAHs were 535.2μg/m3, 157.9μg/m3 and 12.86μg/m3, respectively, with notable health risks, indicating relatively serious pollution in indoor air due to the use of burning moxa. The indoor emissions of target pollutants from burning moxa in test chamber were also investigated. Toluene, benzene and xylene appeared to be dominant MAHCs, and naphthalene (NA) the dominant PAH, which were consistent with the pollution levels of the detected moxibustion rooms. The emission characteristics of smoky moxa and mild moxa were much in common and relatively close to that of tobacco; while that of smoke-free moxa showed a distinction. Though pollutants emission patterns varied within the three types of moxa, all of them had apparently higher emission intensities than other typical indoor sources, including tobacco. The results of this study can offer some references during the selection of moxa sticks and application of moxibustion. © 2014 Elsevier B.V. Source


Zhang Y.,Zhejiang Academy of Traditional Chinese Medicine | Wu R.,Zhejiang Academy of Traditional Chinese Medicine | Hu Y.,Zhejiang Pharmaceutical College | Dong Y.,Zhejiang Academy of Traditional Chinese Medicine | And 5 more authors.
Journal of Mechanics in Medicine and Biology | Year: 2015

Background: Antibiotic-impregnated calcium sulfate delivery systems (ACDS) are commonly used to treat chronic osteomyelitis. Our research is to investigate drug release in vitro over a longer period, as a cautious predictor of in vivo release. Methods: The local release behavior of antibiotic in vitro was simulated. The consecutive dynamic eluting experiment was performed based on the pro-operative characteristic of osteomyelitis patients and the determined results of drug concentration in the human drainage tissue fluid (DTF). The concentration of each drug in the receiving solution was detected by ultra-performance liquid chromatography-tandem quadrupole detector mass spectrometry. The ACDS was reviewed by scanning electronic microscopy (SEM) after 48 h, and prepared to be eluted for another examination after 33 days. The mechanism of antibiotic release was analyzed by using the Ritger-Peppas and Weibull equations. Results: The cumulative release rate of vancomycin in a vancomycin-calcium sulfate delivery system (VCDS) was 77.50 % (3.0 mm diameter) and 72.43 % (4.8 mm diameter), while that of the tobramycin in a tobramycin-calcium sulfate delivery system (TCDS) was 88.0 % (3.0 mm diameter) and 84.55 % (4.8 mm diameter). At the 15th day, approximately 27.92% of vancomycin was and 29.35% of tobramycin was released from the local implant in vivo. Using SEM, numerous vancomycin and tobramycin particles were found to be attached to the columnar calcium sulfate crystals at the start of the experiment. The release behavior of the two antibiotics followed a combination of Fickian diffusion and Case II transport mechanisms within the first 48 h, and a Fickian diffusion mechanism during the subsequent time period. The correlation coefficient of tobramycin and vancomycin in vivo and in vitro was 0.9704-0.9949 and 0.9549-0.9782, respectively. Conclusion: A good correlation of the in vivo and in vitro cumulative release rates was observed by comparing the cumulative release rate of drugs in vitro by means of the dynamic eluting model, and in the DTF. Therefore, our study has proved that it is possible to use the dynamic eluting model as a cautious predictor of in vivo release. © 2015 World Scientific Publishing Company. Source


Zhang Y.,Zhejiang Academy of Traditional Chinese Medicine | Wu R.,Zhejiang Academy of Traditional Chinese Medicine | Hu Y.,P.A. College | Dong Y.,Zhejiang Academy of Traditional Chinese Medicine | And 5 more authors.
Journal of Mechanics in Medicine and Biology | Year: 2014

Background: Antibiotic-impregnated calcium sulfate delivery systems (ACDS) are commonly used to treat chronic osteomyelitis. Our research is to investigate drug release in vitro over a longer period, as a cautious predictor of in vivo release. Methods: The local release behavior of antibiotic in vitro was simulated. The consecutive dynamic eluting experiment was performed based on the pro-operative characteristic of osteomyelitis patients and the determined results of drug concentration in the human drainage tissue fluid (DTF). The concentration of each drug in the receiving solution was detected by ultra-performance liquid chromatography-tandem quadrupole detector mass spectrometry. The ACDS was reviewed by scanning electronic microscopy (SEM) after 48 h, and prepared to be eluted for another examination after 33 days. The mechanism of antibiotic release was analyzed by using the Ritger-Peppas and Weibull equations. Results: The cumulative release rate of vancomycin in a vancomycin-calcium sulfate delivery system (VCDS) was 77.50 % (3.0 mm diameter) and 72.43 % (4.8 mm diameter), while that of the tobramycin in a tobramycin-calcium sulfate delivery system (TCDS) was 88.0 % (3.0 mm diameter) and 84.55 % (4.8 mm diameter). At the 15th day, approximately 27.92% of vancomycin was and 29.35% of tobramycin was released from the local implant in vivo. Using SEM, numerous vancomycin and tobramycin particles were found to be attached to the columnar calcium sulfate crystals at the start of the experiment. The release behavior of the two antibiotics followed a combination of Fickian diffusion and Case II transport mechanisms within the first 48 h, and a Fickian diffusion mechanism during the subsequent time period. The correlation coefficient of tobramycin and vancomycin in vivo and in vitro was 0.9704-0.9949 and 0.9549-0.9782, respectively. Conclusion: A good correlation of the in vivo and in vitro cumulative release rates was observed by comparing the cumulative release rate of drugs in vitro by means of the dynamic eluting model, and in the DTF. Therefore, our study has proved that it is possible to use the dynamic eluting model as a cautious predictor of in vivo release. © 2015 World Scientific Publishing Company. Source


Zhang B.,Zhejiang Provincial Tongde Hospital
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010

To observe the effects of Gutuo Qingfu Decoction (GQD) via gastro-enteric perfusion on blood level of bacterial 16S rRNA gene in severe multi-traumatic (SMT) patients at early stage. Sixty SMT patients were assigned to two groups, the 33 in the treated group and the 27 in the control group. They were treated with the same conventional treatment, but different in the gastro-enteric infusion with GOD for the former and saline for the latter. Blood 16SrRNA gene, body temperature, leukocyte count, C-reactive protein (CRP), and blood bacterial culture positive rate on the 3, 6, 9 post-trauma days were detected, and incidences of infective complication and mortality were observed. Body temperature on day 9 in the treated group was significantly lower than in the control group (37.6 +/- 0.12 degrees C vs 38.1 +/- 0.15 degrees C, P < 0.05); so did the CRP level on day 6 (52.4 +/- 6.3 mg/L vs 104.3 +/- 20.1 mg/L, P < 0.05) and day 9 (42.9 + 7.5 mg/L vs 92.5 +/- 17.1 mg/L, P < 0.05), as well as the positive rates of blood 16SrRNA gene on day 6 and 9 (33.3% vs 59.3% and 30.3% vs 77.8%, P < 0.05 and P < 0.01, respectively). However, the positive rates of blood culture were insignificantly different between the two groups ( P > 0.05). Besides, incidence of infective complication in the treated group was significantly lower than in the control group (30.3% vs 59.3%, P < 0.05). Early stage gastrointestinal administration of GQD is likely to have benefits for the improvement of intestinal mucosa barrier and reduction of enteric bacterial translocation in SMT patients, and it may also reduce the incidence of infective complication in these patients. Source


Shou D.,Zhejiang Academy of Traditional Chinese Medicine | Dong Y.,Zhejiang Academy of Traditional Chinese Medicine | Shen L.,Zhejiang Provincial Tongde Hospital | Wu R.,Zhejiang Academy of Traditional Chinese Medicine | And 3 more authors.
Journal of Chromatographic Science | Year: 2014

Tobramycin and vancomycin are the most commonly used antibiotics for the treatment of osteomyelitis. A sensitive and rapid method was developed for the analysis of tobramycin and vancomycin in human drainage tissue fluid. The procedure involved a simple liquid-liquid extraction of tobramycin, vancomycin and atenolol (internal standard) and separation by ultra-high performance liquid chromatography on an Acquity UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with a mobile phase consisting of 0.1% (v/v) formic acid water solution and 0.1% (v/v) formic acid acetonitrile solution at a flow rate of 0.3 mL/min. Detection was performed by positive ion electrospray ionization in multiple reaction monitoring mode (m/z 468 → 163 transitions for tobramycin; m/z 725 → 144 for vancomycin; m/z 267 → 74 for the internal standard). The retention times of tobramycin, vancomycin, and the internal standard were 0.68, 3.62 and 3.03 min, respectively. The total analysis time was less than 10 min. Excellent linear relationships (correlation coefficient > 0.99) were demonstrated between the area under the peak ratios of tobramycin and vancomycin to the internal standard in the drainage tissue fluid, and the concentration ranges were 1.25-100.00 mg/L and 0.50-150.00 mg/L for tobramycin and vancomycin, respectively. The intra-day and inter-day accuracy and precision (coefficient of variation) acceptance criteria for each quality control was ≤ 7.8% and the mean accuracy values were < 5.0% for tobramycin and < 4.0% for vancomycin. All experiments suggested the high-throughput potential of the proposed method. The method was successfully applied to investigate local delivery of tobramycin and vancomycin in four calcaneal osteomyelitis patients who had accepted drug-loaded artificial bone implantation. © 2013 The Author [2013]. Published by Oxford University Press. All rights reserved. Source

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