Zhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation

Taizhou, China

Zhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation

Taizhou, China
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Wang S.Z.,Southwest University | Wang S.Z.,Chongqing Three Gorges University | Wang S.Z.,Taizhou University | Wang S.Z.,Zhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation | And 8 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2015

Arbuscular mycorrhizal fungi (AMF), which engage in a mutualistic symbiosis with the roots of most plant species, have been well studied for their ability to alleviate heavy metal stress, including that of copper (Cu), in plants. Most mycorrhizal studies have focused on AMF effects on host plant nutrient uptake. However, few studies have investigated plant physiological responses during plant-microbe interactions, which are necessary to understand the efficiency of the phytoremediation of heavy metal pollution. Elsholtzia splendens, a widely distributed Cu-tolerant plant, is usually found in the Cu mining areas of southern China and is colonized by symbiotic AMF. This study examines the effects of AMF on the physiological responses of E. splendens leaves under Cu stress. A controlled pot experiment was performed to investigate the effects of AMF inoculations and Cu additions on E. splendens leaves’photosynthetic pigment contents, anti-oxidation enzymatic activities, antioxidant contents and lipid peroxidation. The plants, which were grown on a mixture of sterilized soil and sand, were inoculated with AMF (non-inoculated plants were controls), with or without the addition of Cu (1000 mg/ kg and 0 mg/ kg, respectively). The results showed that AMF could well colonize the roots of E. splendens under both Cu treatments. The plant root biomass, stem biomass, leaf biomass, inflorescence biomass and total biomass of E. splendens under Cu stress were lower than in the control. Inoculations with AMF significantly increased the plant root biomass, stem biomass, leaf biomass, inflorescence biomass and total biomass of E. splendens under Cu stress by 36.1%, 138.9%, 47.3%, 68. 4% and 79. 7%, respectively, and significantly decreased the root shoot ratio by 26. 3%, indicating an alleviating effect of Cu inhibition on E. splendens growth. Compared with the control, the chlorophyll a (Chl a) and chlorophyll b (Chl b) contents, Chl a+b content [Chl (a+b)], carotenoid content and chlorophyll a/ b ratio (Chl a/ b) under Cu stress significantly decreased by 56.2%, 52.6%, 55.1%, 49.8% and 7.5%, respectively. Additionally, the superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), glutathione (GSH) and ascorbic acid (AsA) contents in the leaves of E. splendens under Cu stress significantly decreased by 44.8%, 45.0%, 61.0%, 43.7%, 49.2% and 31.1%, respectively, while the membrane relative permeability (MRP) and malondialdehyde (MDA) contents significantly increased by 61.5% and 106.4%, respectively. With the Cu addition, the AMF inoculation significantly increased the Chl a, Chl b and Chl (a+b) contents in E. splendens leaves by 36.7%, 30.6% and 34.8%, respectively, and the GSH and AsA contents by 55.1% and 29.6%, respectively. It also enhanced the SOD and APX activities by 33.7% and 66.1%, respectively, while it reduced the MRP and MDA contents by 18.0% and 20.2%, respectively. In conclusion, AMF inoculations could effectively alleviate the damage of Cu stress on E. splendens by increasing the photosynthetic pigment contents and the antioxidant capacities, while decreasing the lipid peroxidation of cell membranes and the relative conductivity, which might be important for Cu stress adaptation and the improved productivity of E. splendens. © 2015, Ecological Society of China. All rights reserved.


Yang B.,Zhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation | Yang B.,Taizhou University | Li J.,Zhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation | Li J.,Taizhou University | And 4 more authors.
Pakistan Journal of Botany | Year: 2015

Parasitic plants have been identified as potential biological agents to control invasive plants. Understanding the interaction between invasive plants and their novel natural enemies is important for understanding mechanisms underlying plant invasion success and thus taking measures to control invasion. We conducted a factorial experiment to test the interactive effects of nutrient addition (low vs. high) and parasitism (with vs. without Cuscuta australis) on the growth of the invasive Bidens pilosa. Parasitism significantly decreased leaf, stem and root biomass of the host invasive plant, and nutrient addition increased leaf and stem biomass of the host. A synergistic effect of parasitism and nutrient addition was found on stem and leaf biomass of the hosts. Nutrient addition significantly increased vegetative biomass of the parasitic plant and caused a more deleterious effect on the invasive host. Reproductive biomass of the parasitic plant was significantly positively related with net photosynthetic rate, light-utilisation efficiency and apparent carboxylation efficiency. Vegetative biomass and total biomass of the parasitic plants were significantly positively related with specific leaf area and the relative chlorophyll content of the host plant. The deleterious effect of the parasite on the growth of the host plant was significantly positively correlated with vegetative biomass of the parasitic plant. Nutrient addition increased the negative effect of the parasitic plant on the invasive host, indicating that the parasitic plant is potentially a biological control agent for the invasive plant even in the context of changing global resources. © 2015, Pakistan Botanical Society. All rights reserved.


Jin Z.,Zhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation | Jin Z.,Taizhou University | Li J.,Zhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation | Li J.,Taizhou University | And 2 more authors.
PLoS ONE | Year: 2015

Plant responses to heavy metal contamination may depend on the presence of arbuscular mycorrhizal fungi (AMF). Elsholtzia splendens is an indicator species for the presence of copper (Cu) mines because both its flowering phenology and reproduction are tolerant to heavy metals. To test whether effects of Cu on the flowering phenology and reproduction of E. splendens depend on the presence of AMF, we conducted a factorial experiment with two Cu treatments (with or without Cu addition) crossed with two AMF treatments (with or without AMF inoculation). Without AMF, Cu addition significantly delayed the onset dates, ending dates and peak dates of flowering and decreased flowering duration. However, AMF inoculation reversed the effects of Cu stress, with recovered flowering onset and ending dates and increased the flowering duration. Cu addition significantly decreased inflorescence width and number, inflorescence biomass, vegetative biomass and total seed number, but significantly increased 1000-seed weight. AMF inoculation significantly increased vegetative biomass. Two-way ANOVA results showed that the interactive effects between Cu addition and AMF inoculation were significant on the inflorescence number, vegetative biomass and total seed number. These results indicate that AMF can alleviate the Cu stress on the flowering phenology and reproduction of E. splendens. © 2015 Jin et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


PubMed | Zhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation
Type: Journal Article | Journal: PloS one | Year: 2015

Plant responses to heavy metal contamination may depend on the presence of arbuscular mycorrhizal fungi (AMF). Elsholtzia splendens is an indicator species for the presence of copper (Cu) mines because both its flowering phenology and reproduction are tolerant to heavy metals. To test whether effects of Cu on the flowering phenology and reproduction of E. splendens depend on the presence of AMF, we conducted a factorial experiment with two Cu treatments (with or without Cu addition) crossed with two AMF treatments (with or without AMF inoculation). Without AMF, Cu addition significantly delayed the onset dates, ending dates and peak dates of flowering and decreased flowering duration. However, AMF inoculation reversed the effects of Cu stress, with recovered flowering onset and ending dates and increased the flowering duration. Cu addition significantly decreased inflorescence width and number, inflorescence biomass, vegetative biomass and total seed number, but significantly increased 1000-seed weight. AMF inoculation significantly increased vegetative biomass. Two-way ANOVA results showed that the interactive effects between Cu addition and AMF inoculation were significant on the inflorescence number, vegetative biomass and total seed number. These results indicate that AMF can alleviate the Cu stress on the flowering phenology and reproduction of E. splendens.

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