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Liu Y.,China Jiliang University | Wang M.,China Jiliang University | Ge J.,China Jiliang University | Ge J.,Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine | Han B.,Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine
Journal of Chinese Institute of Food Science and Technology | Year: 2014

Objective: The study was designed to investigate the distribution of epigallocatechin gallate (EGCG) in mouse liver and testis, basing on establishing a method for determination of EGCG in liver and testis. Then the aim of study was to clarify the effect of catechins on expression difference of CYP3A4 and aromatase. Methods: The RP-HPLC method was used to determine the catechins EGCG in liver and testis. Then the expression difference of CYP3A4 and aromatase mRNA was also detected by qRT-PCR technology and the protein levels of them were analyzed according to the results of Immune fluorescence staining. Results: The results showed that there was a good linearity over the range 0.5~200.0 mg/kg(r>0.99). Then the recoveries were more than 80.0% in liver and testis samples, and the intra- and inter-day coefficients of variation were less than 10.0% in all cases. And catechins were highly distributed in liver and testis tissues. Besides, the expression of CYP3A4 was significantly increased in liver, while the expression of aromatase was significantly decreased in testis. Conclusion: An accurate and reliable method was established for determine the catechins EGCG in liver and testis. And catechins significantly affected the expression of liver CYP3A4 and testis aromatase. Source

Hu H.,China Jiliang University | Hu H.,Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine | Li S.,Zhejiang Chinese Medical University | Liu J.,China Jiliang University | And 3 more authors.
Acta Biochimica et Biophysica Sinica | Year: 2012

MicroRNAs have been reported to be closely related to the development of human lung cancers. However, the functions of microRNAs in non-small cell lung cancer (NSCLC) remain largely undefined. Here, we investigated the role of microRNA-193b (miR-193b) in NSCLC. Our data showed that miR-193b was markedly down-regulated in NSCLC cancer tissues compared with adjacent normal tissues. The NSCLC cell line (A549) transfected with the miR-193b exhibited significantly decreased proliferation, migration, and invasion capacities when compared with the control cells. In contrast, inhibition of miR-193b increased the proliferation, migration, and invasion of A549 cells. Moreover, miR-193b repressed the expressions of cyclin D1 and urokinase-type plasminogen activator in A549 cells. These data suggest that miR-193b is a tumor suppressor in NSCLC. © 2012 The Author. Source

Qian L.,China Jiliang University | Qian L.,Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine | Wenyu Y.,China Jiliang University | Wenyu Y.,Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine | And 8 more authors.
Plant Molecular Biology Reporter | Year: 2011

Zizania latifolia Trucs is a uniquely flavored aquatic vegetable found in southern and eastern Asia. Several physiology and genetic approaches have been employed to increase our knowledge about the physiological basis of gall formation; however, as yet, data at the proteomic level are not available. Protein yield and sodium dodecyl sulfate-polyacrylamide gel electrophoresis were used to determine the most appropriate protein extraction methods for use in this study. Total proteins were extracted from the culm tissue at three relevant developmental stages and separated by two-dimensional gel electrophoresis. The number and abundance of spots varied among the two-dimensional gel electrophoresis gels at the three stages. Proteins with more than 1.7-fold abundance between the different stages were monitored. We identified 10 well-resolved spots by matrix-assisted laser-desorption ionization/time-of-flight peptide mass fingerprinting and tandem mass spectrometry. Some spots linked to signal transduction of the phytohormone could be identified. The expression volume of these spots transiently increased during the expansion phase. In contrast, the spots linked to phytoene dehydrogenase and methionine synthase or pyrophosphate-dependent phosphofructokinase were more abundant during gall formation, showing an increase in spot intensity during development and maximal abundance in mature gall. Higher intensity was also found in the spots linked to stress response. We discuss protein variations, considering their potential role during gall formation and comparing our results with established variations at metabolite-profiling levels. © 2010 Springer-Verlag. Source

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