Zhejiang Provincial Hospital of Traditional Chinese Medicine
Zhejiang Provincial Hospital of Traditional Chinese Medicine
Tao S.,Zhejiang Chinese Medical University |
Qiu T.,Zhejiang Provincial Hospital of Traditional Chinese Medicine
International Journal of Clinical and Experimental Medicine | Year: 2017
Objective: To assess the therapeutic efficacy of thymectomy as a treatment for myasthenia gravis (MG) by meta-analyses. Methods: Through the retrieval of related literature in Chinese National Knowledge Infrastructure database (CNKI), Wan Fang database, CiNii, NCBI, EBSCO, Web of Science and so on, the data, which concerned with thymectomy for myasthenia gravis and published from 2010 to 2016, were obtained. The meta-analysis of operating time, drainage time, hemorrhage volume and therapeutic efficacy were carried out by RevMan5.2 statistical software. Results: Seventeen foreign literature, were included in this study, with 642 cases of the video-assisted thoracic surgery (VATS) group and 603 cases of the sternotomy group. The analysis revealed that the hemorrhage volume of VATS thymectomy group was markedly less than conventional group (RR=6.25, P=0.01), while the drainage time was longer than that of conventional group (RR=1.26, P=0.12). No significant difference of MG improvement rate was found between both groups (RR=1.00, P=0.16). Conclusion: VATS thymectomy is a safe and reliable treatment for myasthenia gravis and it shares the same prostecdtive efficacy with conventional thymectomy. Combined with thoracoscopic surgery, the hemorrhage volume can be decreased and the hospital stay can be shortened in thymectomy. © 2017, E-Century Publishing Corporation. All rights reserved.
Peng L.-Q.,Hangzhou Normal University |
Yu W.-Y.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Xu J.-J.,Hangzhou Normal University |
Cao J.,Hangzhou Normal University
Food Chemistry | Year: 2018
A simple, green and effective extraction method, namely, pyridinium ionic liquid- (IL) based liquid-solid extraction (LSE), was first designed to extract the main inorganic and organic iodine compounds (I−, monoiodo-tyrosine (MIT) and diiodo-tyrosine (DIT)). The optimal extraction conditions were as follows: ultrasonic intensity 100 W, IL ([EPy]Br) concentration 200 mM, extraction time 30 min, liquid/solid ratio 10 mL/g, and pH value 6.5. The morphologies of Laminaria were studied by scanning electron microscopy and transmission electron microscopy. The recovery values of I−, MIT and DIT from Laminaria were in the range of 88% to 94%, and limits of detection were in the range of 59.40 to 283.6 ng/g. The proposed method was applied to the extraction and determination of iodine compounds in three Laminaria. The results showed that IL-based LSE could be a promising method for rapid extraction of bioactive iodine from complex food matrices. © 2017 Elsevier Ltd
Huang S.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Chen L.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Mao Y.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Tong H.,Zhejiang Provincial Hospital of Traditional Chinese Medicine
Medicine (United States) | Year: 2017
Rationale: Primary extracranial meningiomas are rare outside the head and neck region. Patient concerns: A 44-year-old female patient had chest pain for more than 1 year. Diagnoses: Preoperative chest computed tomography (CT) scan revealed a nodule in the right lower lobe, 1.8 cm in diameter. Tumor tissues were examined by immunohistochemistry for vimentin and S-100. Interventions: Histopathologically, the tumor was characterized by whorled nests of spindle-shaped cells accompanied by psammoma bodies. Immunohistochemistry demonstrated tumor cell positivity for vimentin and S-100. This case was diagnosed as a primary pulmonary meningioma. The tumor was removed by a thoracoscopic pulmonary wedge resection. Outcomes: Postoperative cranial and spinal CT scan did not show any intracranial or spinal mass. No recurrence of the tumor was reported at the time of writing up this case report. Lessons: A primary pulmonary meningioma should be considered in the differential diagnosis workup of pulmonary nodules. Copyright © 2017 the Author(s).
Shen S.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Gui T.,Shanghai Smartquerier Biotechnology Co. |
Ma C.,CAS Shanghai Institutes for Biological Sciences |
Ma C.,Shanghai Center for Bioinformatics Technology
Oncotarget | Year: 2017
The high mortality rate of pancreatic cancer makes it one of the most studied diseases among all cancer types. Many researches have been conducted to understand the mechanism underlying its emergence and pathogenesis of this disease. Here, by using minimum-redundancy-maximum-relevance (mRMR) method, we studied a set of transcriptome data of pancreatic cancer. As we gradually added features to achieve the most accurate classification results of Jackknife, a gene set of 9 genes was identified. They were NHS, SCML2, LAMC2, S100P, COL17A1, AMIGO2, PTPRR, KPNA7 and KCNN4. Through STRING 2.0 protein-protein interactions (PPIs) analysis, 40 proteins were identified in the shortest paths between genes in the gene set, 30 of them passed the permutation test, which indicated they were hubs in the background network. Those genes in the protein-protein interaction network were enriched to 37 functional modules, such as: negative regulation of transcription from RNA polymerase II promoter, negative regulation of ERK1 and ERK2 cascade and BMP signaling pathway. Our study indicated new mechanism of pancreatic cancer, suggesting potential therapeutic targets for further study. © Shen et al.
Shou D.-W.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Zheng Y.-L.,Zhejiang Provincial Hospital of Traditional Chinese Medicine
Bangladesh Journal of Pharmacology | Year: 2017
The primary objective of the current work was to investigate the antitumor potential of Primula macrophylla extracts in human colon cancer cell line (Colo-205) along with evaluating the effects on apoptosis induction, cell cycle arrest and mitochondrial membrane potential. Cell viability was assessed by tetrazolium-based MTT assay. Flow cytometry measurement was carried out to assess the effect of the extract on cell cycle phase distribution and mitochondrial transmembrane potential. Results showed that P. macrophylla methanol extract was effective and exhibited highest cell growth inhibition (IC50 value, 26.17 μg/mL). Methanol extract significantly increased the side-scattering profile of Colo-205 cells in concentration-dependent pattern. Exposure of Colo-205 cells with different concentrations of the methanol extract (0-80 μg/mL) caused dose-dependent G0/G1 cell cycle arrest along with inducing apoptotic cascade by increasing the population of cells at G0/ G1 phase. Furthermore, methanol extract treatment caused an increase in mitochondrial membrane depolarization in Colo-205 cells. © 2017, Bangladesh Pharmacological Society. All rights reserved.
Zhang Z.,Yidu Central Hospital of Weifang |
Chen J.,Zhejiang Provincial Hospital of Traditional Chinese Medicine
Medicine (United States) | Year: 2016
Background: The study aimed to compare the effectiveness and safety of topical versus intravenous (IV) tranexamic acid (TXA) for reducing blood loss in primary total hip arthroplasty (THA). Methods: This systematic review and meta-analysis were performed according to the preferred reporting items for systematic reviews and meta-analyses statement criteria. PubMed, Embase, the Cochrane Library, Web of Science, Chinese Biomedicine Literature (CBM), Wanfang Database, China National Knowledge Infrastructure (CNKI), and Google Scholar were searched for randomized controlled trials (RCTs) and non-RCTs that compare topical versus IV-TXA administration for reducing blood loss during TKA from their inception to February, 2016. Meta-analysis was performed by Stata 12.0 software. Results: Seven studies comprising 2056 patients were included in this meta-analysis. No significant difference is found between topical TXA groups and IV-TXA groups regarding transfusion requirements (RR=1.37, 95% confidence interval [CI]: 0.96-1.97, P=0.083), total blood loss (MD 17.09, 95% CI: 33.74-67.91, P=0.510), and hemoglobin decline (MD 0.32, 95%CI: 0.04-0.69, P=0.122). Meanwhile, there is no significant difference in terms of the occurrence of deep venous thrombosis (RR= 1.09, 95% CI: 0.40-3.90, P=0.869). Conclusion: Topical TXA has a similar efficacy to IV-TXA in reducing both blood loss and transfusion rate without sacrificing safety in primary THA. Copyright © 2016 the Author(s). Published by Wolters Kluwer Health, Inc. All.
Zhai L.,Tongde Hospital of Zhejiang Province |
Song Z.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Liu K.,Zhejiang University
Medicine (United States) | Year: 2016
The purpose of this systematic review and meta-Analysis of randomized controlled trials (RCTs) and non-RCTs was to evaluate the efficacy and safety of gabapentin versus placebo for pain control after total knee arthroplasty (TKA). In December 2015, a systematic computer-based search was conducted in the Medline, Embase, PubMed, Cochrane Controlled Trials Register (CENTRAL), Web of Science, Google, and Chinese Wanfang databases. This systematic review and meta-Analysis were performed according to the preferred reporting items for systematic reviews and meta-Analyses (PRISMA) statement criteria. The primary endpoint was the visual analogue scale (VAS) score after TKA with rest or mobilization at 24 and 48hours, representing the efficacy of pain control after TKA. Cumulative morphine consumption via patient controlled anesthesia (PCA) was also assessed to determine the morphine-spare effect. Complications such as dizziness, pruritus, vomiting, nausea, and sedation were also compiled to assess the safety of gabapentin. Stata 12.0 software was used for the meta-Analysis. After testing for publication bias and heterogeneity across studies, the data were aggregated for random-effects modeling whenever necessary. Six studies involving 769 patients met the inclusion criteria. Our meta-Analysis revealed that gabapentin resulted in superior pain relief compared to the control group in terms of VAS score with rest at 24hours (mean difference [MD]=-3.47; 95% confidence interval [CI]-6.16 to-0.77; P=0.012) and at 48hours postoperatively (MD=-2.25; 95% CI-4.21 to-0.30; P=0.024). There was no statistically significant difference between the groups with respect to the VAS score at 24hours postoperatively (MD=1.05; 95% CI-3.31 to 5.42; P=0.636) or at 48hours (MD=1.71; 95% CI-0.74 to 4.15; P=0.171). These results indicated that the perioperative administration of gabapentin decreases the cumulative morphine consumption via PCA at 24hours (MD=-8.28; 95% CI-12.57 to-3.99; P=0.000) and 48hours (MD=-4.50; 95% CI-10.98 to-3.61; P=0.221). Furthermore, gabapentin decreased the rate of postoperative dizziness (relative risk [RR], 0.68; 95% CI 0.47-0.99, P=0.044) and the occurrence of pruritus (RR, 0.50; 95% CI 0.37-0.67, P=0.000). Based on the current meta-Analysis, gabapentin exerts an analgesic and opioid-sparing effect in acute postoperative pain management without increasing the rate of dizziness and pruritus. © 2016 Wolters Kluwer Health, Inc. All rights reserved.
Xie X.-H.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Zhao H.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Hu Y.Y.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Gu X.D.,Zhejiang Provincial Hospital of Traditional Chinese Medicine
Experimental and Therapeutic Medicine | Year: 2014
Multidrug resistance (MDR) is a major obstacle to the chemotherapeutic treatment of breast cancer. Germacrone, the main component of Rhizoma Curcuma, has been shown to possess antitumor, anti‑inﬂammatory and immunomodulatory properties. The aim of the present study was to investigate the effect of germacrone on MCF‑7/Adriamycin (ADR) multidrug‑resistant human breast cancer cells. The treatment of MCF‑7/ADR cells with a combination of germacrone and ADR resulted in an increase in cytotoxicity compared with that of ADR alone, as determined using an MTT assay. Flow cytometric analysis revealed that germacrone promoted cell apoptosis in a dose‑dependent manner, whilst treatment with germacrone plus ADR enhanced the apoptotic effect synergistically. Furthermore, the results from the western blot analysis demonstrated that augmenting ADR treatment with germacrone resulted in a reduction of anti‑apoptotic protein expression levels (bcl‑2) and enhancement of pro‑apoptotic protein expression levels (p53 and bax) in MCF‑7/ADR cells compared with the levels achieved by treatment with ADR alone. In addition, germacrone significantly reduced the expression of P‑glycoprotein via the inhibition of the multidrug resistance 1 (MDR1) gene promoter. These findings demonstrate that germacrone has a critical role against MDR and may be a novel MDR reversal agent for breast cancer chemotherapy. © 2014, Spandidos Publications. All rights reserved.
Zhou J.G.,Zhejiang Provincial Hospital of Traditional Chinese Medicine
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences | Year: 2011
To determine the role of the novel proinflammatory cytokine high mobility group box chromosomal protein 1 (HMGB-1) in the pathogenesis of lupus nephritis. Serum levels of anti-dsDNA antibodies were determined by enzyme linked immunosorbent assay (ELISA). Renal morphologic features were examined by light microscopy, electron microscopy, and immunohistologic analyses. The mRNA expression of HMGB-1 and monocyte chemoattractant protein-1 (MCP-1) was detected by RT-PCR. MRL/lpr mice demonstrated characteristic alterations of serum immune parameters, with progressively increased anti-dsDNA antibodies with age, compared with age-matched C57BL/6J mice. MRL/lpr mice showed progressive development of renal damage, starting at 12 weeks of age and reached the peak at 20 weeks. The observed lesions included the presence of enlarged hypercellular glomeruli, with increased numbers of both resident cells and infiltrating leukocytes. Higher expression of HMGB-1 mRNA was found in MRL/lpr mice than what in C57BL/6J mice. Expression of HMGB-1 was positively correlated with that of MCP-1 mRNA. The results demonstrate that the higher expression of HMGB-1 may contribute to the pathogenesis of lupus nephritis.
Jin C.,Zhejiang University |
Liang R.,Zhejiang Provincial Hospital of Traditional Chinese Medicine
Journal of Obstetrics and Gynaecology Research | Year: 2015
Aim AKT signaling regulates multiple biological processes and expresses in various cancers. miR-205 plays complex roles in tumorigenesis and tumor progression by acting either as a tumor suppressor or an oncogene depending on the tumor type. Here we describe the molecular mechanism of miR-205 regulating epithelial-mesenchymal transition by activation of AKT signaling in endometrial cancer cells HEC-50B and HEC-1-A. Material and Methods The proliferation of HEC-50B cells transfected with miR-205 mimic was assessed by WST-1 assay. The migration and invasion were evaluated by BD transwell migration and matrigel invasion assays. The EMT markers were detected by Western blot. Results We found that miR-205 increased the proliferation in HEC-50B cells. The migration and invasion of HEC-50B cells and HEC-1-A cells were enhanced by miR-205. When HEC-50B cells and HEC-1-A cells were treated with anti-miR-205 inhibitor, the migration and invasion were decreased as compared with the negative control. The overexpression of miR-205 inhibited E-cadherin expression and promoted Snail expression by activation of AKT and downregulation of glycogen synthase kinase 3β. However, after the HEC-50B cells and HEC-1-A cells were treated with anti-miR-205 inhibitor, E-cadherin expression was increased and Snail protein level was decreased by inhibition of AKT expression. Conclusion Our data strongly suggest that miR-205 plays an important role in endometrial cancer migration and invasion by targeting the AKT pathway. Our data highlight miR-205 as a potential molecular target for endometrial cancer treatment. © 2015 Japan Society of Obstetrics and Gynecology.