Zhejiang Metallurgical Research Institute

Hangzhou, China

Zhejiang Metallurgical Research Institute

Hangzhou, China
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Yan M.C.,Zhejiang Metallurgical Research Institute
Dong wu xue yan jiu = Zoological research / "Dong wu xue yan jiu" bian ji wei yuan hui bian ji | Year: 2012

Nibea albiflora was immunized by intraperitoneal injection with either Lipopolysaccharide (LPS) or outer membrane protein (OMP) extracted from Vibrio vulnificus or formalin killed Vibrio vulnificus (FKC). The influence of the three antigens on the immunological function of Nibea albiflora was determined at different time points following the injection by testing the agglutinating antibody titers of the serum, lysozyme activity of the serum, phagocytic activity of the blood and the relative survival percentage. The results showed that the three antigens have higher immunogenicity and antigenicity than the control group(injection with sterile saline). The agglutinating antibody titers of the immune challenged groups increased quickly, and were highest on the day 28. The lysozyme activity and phagocytic activity were raised significantly (P<0.01), reaching their top value on day 21, and then the index gradually reduced. The immunological indexes of three immune groups were higher than the control group (P<0.05). The agglutinating antibody titers of the LPS group or the OMP group were lower than the control group, but the relative survival percentage was adverse when challenged with a Vibrio vulnificus infection. The order of relative survival percentage was group LPS>OMP>FKC>Control.

Yue X.,CAS Qingdao Institute of Oceanology | Huan P.,CAS Qingdao Institute of Oceanology | Xiao G.,Zhejiang Metallurgical Research Institute | Liu B.,CAS Qingdao Institute of Oceanology
Developmental and Comparative Immunology | Year: 2013

An i-type lysozyme (MmeLys) has been proved to function in immunity of the clam Meretrix meretrix in our previous studies. In this study, the expression patterns of MmeLys mRNA and protein at four chief developmental stages of M. meretrix were analyzed, which was able to provide information about how ontogeny of immunity and, in particular, antibacterial ability occured in the bivalve. The results of real-time PCR and western blot showed that MmeLys expressions were activated in D-veligers and dramatically increased to the highest level in pediveligers. It is proposed that the expression changes at these two stages might be due to the visceral organs changes, which were related to the archenteron formation in D-veligers and the organ-restructuring in pediveligers during metamorphosis. In addition, new methods of whole mount in situ hybridization and whole mount immunofluorescence were applied to identify the MmeLys expression tissues, and these tissues (i.e. hepatopancreas, gill, mantle, mouth, velum and foot) may be involved in the immune function during development of clams. Our study is valuable to a certain extent for exploring the origin of immune functions in clams and provides new methodology for future studies on the immune ontogeny of bivalves. © 2013 Elsevier Ltd.

Li H.,Zhejiang University | Wang X.,Zhejiang University | Dong Z.,Zhejiang Metallurgical Research Institute | Xu L.,Zhejiang University | Chen C.,Zhejiang University
Journal of Alloys and Compounds | Year: 2010

With the development of hydrogen storage technique using lightweight high-pressure hydrogen storage tank, high efficient hydrogen compression is of great interest. The objective of this study is to produce hydrogen with pressure of over 70 MPa by using a thermally driven hydride compressor. To reach this target, the hydrogen storage properties of La-Ce-Ca-Ni-Al family AB5 type and Ti-Zr-Cr-Fe-V family AB2 type multicomponent alloys have been investigated. La0.35Ce0.45Ca0.2Ni 4.95Al0.05 alloy was developed as the first stage hydrogen compression alloy, and Ti0.8Zr0.2Cr0.95Fe 0.95V0.1 as the second stage hydrogen compression alloy. With these two alloys, a double-stage compressor with hydrogen compression capacity of around 2000 L/cycle was designed and built, oil was used as the cooling and heat medium. When hot oil of 423 K is used as the heat source, product hydrogen with pressure of 74.5 MPa can be obtained, and the hydrogen flow rate of the compressor reaches 34.6 L/min. © 2010 Elsevier B.V.

Liu H.,Zhejiang University | Wang X.,Zhejiang University | Liu Y.,Zhejiang University | Dong Z.,Zhejiang Metallurgical Research Institute | And 3 more authors.
Journal of Materials Chemistry A | Year: 2013

Organometallically prepared AlH3 and as-received Al powders were mixed with MgH2 to improve the dehydriding and rehydriding properties of MgH2. Thermal analysis shows that the onset dehydriding temperature of MgH2 is reduced by 55 °C (or 25 °C) when mixed with AlH3 (or Al). The destabilization of MgH2 is attributed to the formation of Mg-Al alloys through the reaction between MgH2 and Al. Isothermal dehydriding measurements demonstrate that AlH3 and Al both improve the dehydriding kinetic of MgH2 to some extent, and it only takes 44 min for MgH2 + AlH3 (5.4 h for MgH2 + Al) to release 60% of the hydrogen of MgH 2 at 300 °C, but 8.6 h are required for as-milled MgH 2. The apparent activation energy for the dehydriding of MgH 2 is reduced from 174.6 kJ mol-1 for as-milled MgH 2 to 154.8 and 138.1 kJ mol-1 for MgH2 mixed with Al and AlH3 respectively; this is responsible for the improvement in the dehydriding kinetics of MgH2. Despite this, AlH3 is better in destabilizing MgH2 than the as-received Al for the fact that Al* formed in situ from the decomposition of AlH 3 is oxide-free on the particle surfaces, which effectively increases the chemical activity of Al*. Furthermore, the brittleness of AlH 3 makes it easier to mix MgH2 with AlH3, which would result in uniform distributions of Mg and Al and shortening of the diffusion length. Concerning the reversibility, at 300 °C and 5 MPa H 2 and MgH2 are fully recovered in the dehydrided MgH 2 + Al, and MgH2 + AlH3 samples after rehydriding for 10 h. The rehydriding kinetic of MgH2 is significantly enhanced for the dehydrided MgH2 + AlH3, but not in the case of the MgH2 + Al. © 2013 The Royal Society of Chemistry.

Liu H.,Zhejiang University | Wang X.,Zhejiang University | Liu Y.,Zhejiang University | Dong Z.,Zhejiang Metallurgical Research Institute | And 3 more authors.
Journal of Physical Chemistry C | Year: 2014

In this work, the hydrogen desorption properties of the novel Mg-Al-H hydrogen storage composites prepared by ball-milling the as-received magnesium hydride (MgH2) and the as-prepared aluminum hydride (AlH3) with different molar ratios (1:1, 1:0.5, and 1:0.25) are investigated systematically. Nonisothermal desorption measurements show that more than 7.51 wt % of hydrogen is released from the Mg-Al-H composites when they are heated from room temperature to 500 C. Thermal analysis by differential scanning calorimeter (DSC) and hydrogen desorption spectrum by mass spectrometer (MS-H2) are used synchronously to study the desorption process of the Mg-Al-H. The onset hydrogen desorption temperature of MgH2 in the MgH2 + AlH3 composite is dramatically reduced from 320 C for the pure MgH2 to below 252 C. The DSC/MS-H2 and the X-ray diffraction (XRD) analyses show that the desorption of MgH2 in the Mg-Al-H composite is composed of two steps with the reaction between MgH2 and Al forming Mg17Al12 as the first step and the self-decomposition of the residual MgH2 as the second step. Isothermal desorptions show that the desorption kinetics of the MgH2 in the Mg-Al-H composite is significantly improved. The apparent activation energy for the desorption of MgH2 in the MgH2 + 0.25AlH3 is reduced to 144.6 kJ/mol, which contributes to the improvement in the desorption kinetics of MgH2. The Johnson-Mehl-Avrami (JMA) kinetic studies indicate that the desorption kinetic model of MgH2 in the Mg-Al-H composite is different from the pure MgH2. Concerning the reversibility, under the conditions of 300 C and 5 MPa H2, MgH2 in the Mg-Al-H composite is almost fully recovered after absorption for 5 h while it is partially recovered for the pure MgH2. Among the samples studied, the MgH2 + 0.25AlH 3 composite exhibits the highest reversible hydrogen storage capacity of 6.10 wt % (H/M). © 2013 American Chemical Society.

Wei X.J.,Zhejiang Province Key Laboratory of Nobel Metal Catalytic Materials and Technology | Pan J.M.,Zhejiang Metallurgical Research Institute
Advanced Materials Research | Year: 2014

A novel method for the determination of rhodium in waste rhodium-loaded carbon catalyst samples was established by inductively coupled plasma atomic emission spectrometry after samples digested by Teflon pressure digestion vessel with aqua regia. Such experiment conditions were investigated as the influence of sample dissolution methods, digestion time, digestion temperature and interfering ions on the determination. The results indicated that the digestion effect was optimum with samples digested 8 hour at 180 °C. Al, Fe, Mg, Ca and Si in samples had no effect on the determination of rhodium. Experiment indicated rhodium concentration within the range of 0 ~ 150 mg L-1 had a linear relation with emission intensity. The recovery was 99.20% ~ 100.73%, and the relative standard deviation was 1.8%. A satisfactory result was obtained when applying the proposed method in the practical sample. © (2014) Trans Tech Publications, Switzerland.

Ma A.,Zhejiang Metallurgical Research Institute
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2010

OBJECTIVE: We isolated bacterial strain named M-1 from ill Sciaenops ocellatus with ulceration, and further analyzed the phylogenetics of the bacterial strain. METHODS: We characterized the strain on the basis of its morphology, physiology, and sequence comparison of 16SrRNA and HSP60 genes. RESULTS: Strain M-1 was identified as Vibrio splendidus. 16SrRNA gene of strain M-1 and Vibrio splendidus (AJ874361) had nearest kinship, and the analysis of HSP60 gene sequence indicated that strain M-1 and Vibrio (EU653883, AY837440) located in the same phylogenetic tree. CONCLUSION: The results suggested that strain M-1 was a member of Vibrio splendidus.

Wang H.,CAS Qingdao Institute of Oceanology | Chai X.,Zhejiang Metallurgical Research Institute | Liu B.,CAS Qingdao Institute of Oceanology
Aquaculture Research | Year: 2011

The aim of this study was to estimate the genetic parameters of the growth performance of clam Meretrix meretrix. As part of the breeding programme, 25 full-sib families nested within eight half-sib families were produced, planted out and tested to assess the heritability of the total body weight (TW), shell length (SL), shell height (SH) and shell width (SW) at different growth stages. Method of analysis was implemented using the Bayesian method based on the Multiple Trait using Gibbs Sampling under Animal Model program, which was used to estimate the (co)variance components of the traits and conduct genetic analysis. A total of 25 full-sib families, each with 30-35 individuals within family, were used for the analysis. Significant positive genetic and phenotypic correlations between SL, SW, SH and TW with each other were observed. The growth traits showed high-magnitude heritabilities, with values ranging from 0.64 to 0.85, which indicates that these traits should respond to selection and therefore should be included in genetic improvement programmes. © 2010 The Authors. Aquaculture Research © 2010 Blackwell Publishing Ltd.

Shen Y.-Y.,Xiamen University | Chen X.,Zhejiang Metallurgical Research Institute | Murphy R.W.,CAS Kunming Institute of Zoology | Murphy R.W.,Royal Ontario Museum
PLoS ONE | Year: 2013

In recent years, the number of sequences of diverse species submitted to GenBank has grown explosively and not infrequently the data contain errors. This problem is extensively recognized but not for invalid or incorrectly identified species, sample mixed-up, and contamination. DNA barcoding is a powerful tool for identifying and confirming species and one very important application involves forensics. In this study, we use DNA barcoding to detect erroneous sequences in GenBank by evaluating deep intraspecific and shallow interspecific divergences to discover possible taxonomic problems and other sources of error. We use the mitochondrial DNA gene encoding cytochrome b (Cytb) from turtles to test the utility of barcoding for pinpointing potential errors. This gene is widely used in phylogenetic studies of the speciose group. Intraspecific variation is usually less than 2.0% and in most cases it is less than 1.0%. In comparison, most species differ by more than 10.0% in our dataset. Overlapping intra- and interspecific percentages of variation mainly involve problematic identifications of species and outdated taxonomies. Further, we detect identical problems in Cytb from Insectivora and Chiroptera. Upon applying this strategy to 47,524 mammalian CoxI sequences, we resolve a suite of potentially problematic sequences. Our study reveals that erroneous sequences are not rare in GenBank and that the DNA barcoding can serve to confirm sequencing accuracy and discover problems such as misidentified species, inaccurate taxonomies, contamination, and potential errors in sequencing. © 2013 Shen et al.

Hu L.,Zhejiang Metallurgical Research Institute | Jin X.,Zhejiang Metallurgical Research Institute | Shi L.,Zhejiang Metallurgical Research Institute | Li S.,Anhui University of Technology
Advanced Materials Research | Year: 2013

A 3-D thermal-mechanical model was built to simulate the hot rolling process of medium plate, with the aid of nonlinear commercial FE code MSC.SuperForm on a company's actual process parameters. The hot rolling process of single-pass which slab thickness is 180mm was simulated, and the influence of pass reduction on metal flow, stress-strain field, contact stress and rolling force were researched. The study revealed that pass reduction should be at least 20% by increase depress in pass in addition to rolling efficiency. As that, rolling efficiency be increased, roll contact stress be brought down, and its service life be prolonged. And metal plastic strain enhanced, metal flow increased, but its strain field non-uniformly distributed, metal flow and plastic deformation would be strengthen by increase pass reduction, and the lateral broadening in the head is bigger than that in the tail. © (2013) Trans Tech Publications, Switzerland.

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