Zhejiang Marine Development Research Institute

Zhoushan, China

Zhejiang Marine Development Research Institute

Zhoushan, China
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Zhang B.,Zhejiang Ocean University | Yang H.-C.,Zhejiang Marine Development Research Institute | Tang H.,Zhejiang Ocean University | Hao G.-J.,Zhejiang Ocean University | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2017

The cryoprotective effects of carrageenan oligosaccharides on peeled whiteleg shrimp were investigated and compared with sodium pyrophosphate treatment during frozen storage, primarily the interaction mechanisms between oligosaccharides and shrimp myosin. Data revealed significant profitable effects on water-holding capacity and textural variables in oligosaccharide-treated shrimp compared to the control. Chemical analyses showed that these saccharides maintained a higher myofibrillar protein content and Ca2+-ATPase activity in frozen shrimp. Additionally, the hematoxylin and eosin staining results indicated that the saccharides significantly slowed the damage to muscle tissue structures. The assumption was that water replacement hypothesis played a leading role in cryoprotection of frozen shrimp. Furthermore, the homology modeling and molecular dynamics simulations confirmed that the saccharides substituted water molecules around the shrimp myosin surface by forming hydrogen bonds with polar residues of amino acids, thereby stabilizing the structures in the absence of water, leading to an increase in protein stability during frozen storage. © 2017 American Chemical Society.


Fu W.,Zhejiang Marine Development Research Institute | Zhang F.,Zhejiang Ocean University | Liao M.,Zhejiang Marine Development Research Institute | Liu M.,Zhoushan Fisheries Research Institute | And 3 more authors.
Fish and Shellfish Immunology | Year: 2013

Heat shock protein 70s (Hsp70s) play important roles in resisting environmental stresses and stimulating innate immune system. To understand the immune defense mechanisms of Scylla serrata, a full-length cytosolic Hsp70 cDNA of S. serrata (designated as SSHsp70) was obtained by reverse transcriptase-polymerase chain reaction (RT-PCR) coupled with rapid amplification of cDNA ends (RACE). The full-length of SSHsp70 cDNA was 2235 bp, with a 5' untranslated region of 105 bp, a 3' untranslated region of 174 bp, and an open reading frame of 1956 bp encoding a polypeptide of 651 amino acids with an estimated molecular mass of 71.3 kDa and an estimated isoelectric point of 5.55. The cloned SSHsp70 belonged to a cytosolic Hsp70 family. Three typical Hsp70 signature motifs were detected in SSHsp70 by InterPro analysis. Quantitative PCR (qPCR) was used to detect tissue distribution and mRNA expression levels of SSHsp70 under different stress conditions. The obviously high levels of SSHsp70 transcript were in hemocyte, heart, hepatopancreas and gill, whereas low levels were detected in muscle, eyestalk, stomach, and gut. In different temperature treatments, the expression levels of SSHsp70 in low or high temperatures were higher than those in temperate temperature. In pathogen challenge treatments, the mRNA expression level of SSHsp70 reached a maximum level after 18 h and then dropped progressively. In different salt concentration treatments, the mRNA expression level of SSHsp70 had a minimum level at 25‰ salt concentration and high expression levels at high or low salt concentration. In different nitrite concentration treatments, the mRNA expression level of SSHsp70 increased progressively with the increase of nitrite concentration. The results confirmed Hsp70 could be used as a tool for evolution and phylogenetic analysis, a kind of potential biomarker, and a disease resistance factor used in application. © 2013 Elsevier Ltd.


Xu H.,Zhejiang Ocean University | Ying D.,Zhejiang Ocean University | Lu A.,Zhejiang Marine Development Research Institute | Wang X.,Zhejiang Ocean University | Hu J.,Zhejiang Marine Development Research Institute
Superlattices and Microstructures | Year: 2015

CaWO4:Eu3+ phosphors with different morphologies were synthesized by the surfactant-assistant solvothermal process. The structure and luminescent properties were characterized by XRD, SEM, TEM, IR, XPS, and spectrophotometer. The XRD and IR results show that the samples have the scheelite phase. The XPS result shows that Eu3+ ions have doped into CaWO4 hosts successfully. PEG-400, En, and EDTA play the key roles in the formation of microspheres with smooth surface, microspheres with rough surface, and microoctahedrons, respectively. All samples show emission bands originating from the 5D0 → 7Fj (j = 1, 2, 3, 4) transitions of Eu3+ ions. The morphology has obvious influence on the emission intensity. The microspheres with smooth surface have the highest emission intensity, and the microoctahedrons have the lowest emission intensity. © 2015 Elsevier Ltd.


PubMed | Zhejiang Marine Development Research Institute, Zhejiang Ocean University and Guangxi University
Type: | Journal: International journal of biological macromolecules | Year: 2016

Sulfated polysaccharides extracted from brown marine algae have been shown to possess a variety of biological activities. We assessed the potential activity of the sulfated polysaccharide from Sargassum horneri (SP) and its isolated two major components (fraction-1 (F1) and fraction-2 (F2)), on anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. In the present study, analysis of polysaccharide chemical composition found that the constituent ratios of sulfate ester and fucose in SP and F1 were 4.95% vs 7.6%, and 4.48% vs 55.9%, respectively, suggesting that F1 may be a major sulfated polysaccharide containing fucose. Meanwhile, our findings demonstrated that TNF- secretion levels were significantly (P<0.05) decreased by SP and F1 treatments in LPS-stimulated RAW264.7 cells in a dose-dependent manner under the preventive and repair experimental models. Pro-/anti-inflammatory (TNF-/IL-10) cytokines secretion ratios by LPS-stimulated RAW264.7 macrophages were significantly (P<0.05) inhibited by SP and F1 treatments, particularly by F1 (at high dose, 200g/ml). Moreover, NO release and iNOS activity were significantly (P<0.05) inhibited by F1. Collectively, the present study suggested that purified component, F1 from SP, had strong anti-inflammatory effects on LPS-stimulated RAW264.7 macrophages in the preventive and repair manner through inhibiting TNF- secretion levels and NO release.


PubMed | Zhejiang Marine Development Research Institute, Zhejiang Ocean University and Guangxi University
Type: Journal Article | Journal: Marine drugs | Year: 2015

Chitosan and its derivatives such as low molecular weight chitosans (LMWCs) have been reported to exert many biological activities, such as antioxidant and antitumor effects. However, complex and molecular weight dependent effects of chitosan remain controversial and the mechanisms that mediate these complex effects are still poorly defined. This study was carried out to investigate the immunostimulative effect of different molecular weight chitosan in RAW264.7 macrophages. Our data suggested that two LMWCs (molecular weight of 3 kDa and 50 kDa) both possessed immunostimulative activity, which was dependent on dose and, at the higher doses, also on the molecular weight. LMWCs could significantly enhance the the pinocytic activity, and induce the production of tumor necrosis factor (TNF-), interleukin 6 (IL-6), interferon- (IFN-), nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in a molecular weight and concentration-dependent manner. LMWCs were further showed to promote the expression of the genes including iNOS, TNF-. Taken together, our findings suggested that LMWCs elicited significantly immunomodulatory response through up-regulating mRNA expression of proinflammatory cytokines and activated RAW264.7 macrophage in a molecular weight- and concentration-dependent manner.


PubMed | Zhejiang Marine Development Research Institute, Zhoushan Hospital Hospital of Chinese Medicine and Orthopedics, Zhejiang Ocean University and Zhejiang University
Type: Journal Article | Journal: Marine drugs | Year: 2016

Chitosan and its derivatives such as low molecular weight chitosans (LMWCs) have been found to possess many important biological properties, such as antioxidant and antitumor effects. In our previous study, LMWCs were found to elicit a strong immunomodulatory response in macrophages dependent on molecular weight. Herein we further investigated the molecular weight-dependent immunostimulative activity of LMWCs and elucidated its mechanism of action on RAW264.7 macrophages. LMWCs (3 kDa and 50 kDa of molecular weight) could significantly enhance the mRNA expression levels of COX-2, IL-10 and MCP-1 in a molecular weight and concentration-dependent manner. The results suggested that LMWCs elicited a significant immunomodulatory response, which was dependent on the dose and the molecular weight. Regarding the possible molecular mechanism of action, LMWCs promoted the expression of the genes of key molecules in NF-B and AP-1 pathways, including IKK, TRAF6 and JNK1, and induced the phosphorylation of protein IKB in RAW264.7 macrophage. Moreover, LMWCs increased nuclear translocation of p65 and activation of activator protein-1 (AP-1, C-Jun and C-Fos) in a molecular weight-dependent manner. Taken together, our findings suggested that LMWCs exert immunostimulative activity via activation of NF-B and AP-1 pathways in RAW264.7 macrophages in a molecular weight-dependent manner and that 3 kDa LMWC shows great potential as a novel agent for the treatment of immune suppression diseases and in future vaccines.


Fu W.,Zhejiang Marine Development Research Institute | Fu W.,CAS Qingdao Institute of Oceanology | Shuai L.,Qingdao University | Yao J.,CAS Qingdao Institute of Oceanology | And 3 more authors.
Journal of Applied Phycology | Year: 2011

In this study, homologous cloning coupled with the rapid amplification of cDNA ends was used to clone a full-length cytosolic heat shock protein 70 of Ulva pertusa (designated as UPHsp70). Bioinformatics was used to analyze structural features, homologous relationship, and phylogenetic position of UPHsp70. The full length of UPHsp70 cDNA was 2,283 bp, with a 5′ untranslated region of 65 bp, a 3′ untranslated region of 247 bp, and an open reading frame of 1,971 bp encoding a polypeptide of 656 amino acids with an estimated molecular weight of 71.13 kDa and an estimated isoelectric point of 5.04. The UPHsp70 had four degenerate repeats of tetrapeptide GGMP and three typical Hsp70 signature motifs. The specific C-terminus amino acid sequence of cytosolic UPHsp70 was EEVD, and the conservation of Hsp70s of N-terminus was higher than that of C-terminus. The homology between UPHsp70 and Hsp70s of other known algae and land plants was more than 70%. Under different stress conditions, mRNA expression levels of UPHsp70 were quantified by quantitative reverse transcriptase-polymerase chain reaction. When U. pertusa samples were kept in different temperatures (5-40°C) for 1 h, the expression level of UPHsp70 at 5°C, 35°C, or 40°C was over onefold higher than that at 25°C. When U. pertusa samples were kept at 30°C for different times (0-12 h), the mRNA expression level of UPHsp70 had a trend of rise first then fall. The expression level of UPHsp70 reached maximum level after 5 h. When U. pertusa samples were kept in different salt concentrations (0-45‰) for 2 h, the expression level of UPHsp70 at 0‰ or 5‰ salt concentration was twofold higher than that at 30‰ for 2 h. The expression levels of UPHsp70 at 30‰, 35‰, and 40‰ were low and had no difference (P < 0. 05). When U. pertusa samples were kept at ultraviolet irradiation or desiccated for different times (0-4 h), the mRNA expression level of UPHsp70 reached maximum level after 3.0 h; after that, it was maintained at high level. © 2010 Springer Science+Business Media B.V.


Zhang X.,Zhejiang GongShang University | Zhang X.,Zhejiang Marine Fisheries Research Institute | Zheng B.,Zhejiang Marine Development Research Institute | Zhang H.,Zhejiang GongShang University | And 2 more authors.
Journal of Separation Science | Year: 2011

Methyl-3-quinoxaline-2-carboxylic acid (MQCA) is the last major remaining detectable metabolite of Olaquindox in animal tissue. A rapid, sensitive and specific ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the detection and quantification of MQCA in fish tissue using deuterated quinoxaline-2-carboxylic acid (d 4-QCA) as internal standard. Various parameters affecting sample preparation, LC separation and MS/MS detection were investigated, and the optimal conditions concerned were determined. Fish tissue samples were subject to hydrochloric acid hydrolysis followed by Oasis MAX solid-phase extraction clean-up; analysis was performed using UPLC coupled to electrospray MS/MS. The chromatographic separation was achieved in less than 5 min. The limit of detection and the limit of quantification were 0.1 and 0.25 ng/g, respectively. The average recoveries of MQCA, spiked at levels of 0.25-50.0 ng/g, were from 92.7 to 104.3%. The relative standard deviation values were <6%. The validated method was successfully applied to analyze 60 batch samples collected from the local market. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Yang H.,Ocean University of China | Yang H.,Zhejiang Marine Development Research Institute | Zeng M.,Ocean University of China | Dong S.,Ocean University of China | And 2 more authors.
Chinese Journal of Oceanology and Limnology | Year: 2010

In this study, we evaluated the anti-proliferative activity of phlorotannins derived from brown algae Laminaria japonica Aresch extracts on the human hepatocellular carcinoma cell (BEL-7402) and on murine leukemic cells (P388) by MTT assay. Cells were incubated with 100 μg/mL of the phlorotannin extract (PE) for 48 h. The inhibitory rate of PE on BEL-7402 and P388 cells was 30.20±1.16% and 43.44±1.86%, respectively, and the half-inhibitory concentration of PE (IC 50) on P388 and BEL-7402 cells was 120 μg/mL and >200 μg/mL, respectively. Microscopic observation shows that the morphologic features of tumor cells treated with PE and 5-fluorouracil are markedly different from the normal control group. The inhibitory rate of fraction A 2 isolated from PE by sephadex LH-20 for BEL-7402 and P388 cells at the sample concentration of 70.42 μg/mL was 61.96±7.02% and 40.47±8.70%, respectively. The apoptosis peak for fraction A 2 was the most profound of all fractions used in the flow cytometry assay. The results indicate that the anti-proliferative of this algal extract is associated with the total phlorotannin content. © 2010 Chinese Society for Oceanology and Limnology, Science Press and Springer Berlin Heidelberg.


Xu C.,Zhejiang University | Chen J.,Zhejiang University | Yan D.,Zhejiang University | Ji J.,Zhejiang Marine Development Research Institute
Journal of Atmospheric and Oceanic Technology | Year: 2016

Underwater cables play vital roles in marine engineering because they provide power and communication connections from the shore to an increasing number of sea installations. To ensure the system is operating reliably and continuously, it is necessary to detect the shapes of underwater cables in real time. However, this task is difficult to accomplish because the underwater cables are located in a dynamic and complicated subsea environment, which can cause changes in position, depth, and visibility. In this report, the current development of underwater cable shape detection methods, including visual, acoustic, magnetic detection, and multisensor fusion detection, and the advantages and disadvantages are described and analyzed. Furthermore, the disadvantages of these methods are addressed, which, based on survey platforms with high cost, include a long detection period and the failure to reveal emergencies. Then, the need to construct a simple and reliable system to detect the shapes of underwater cables is highlighted, and one possible solution based on bend sensors embedded in underwater cables is discussed. © 2016 American Meteorological Society.

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