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Liu B.,Zhejiang Metallurgical Research Institute | Liu B.,Zhejiang Key Laboratory of Exploitation and Reservation of Coastat Bio Resource | Liu B.,Chinese Academy of Fishery Sciences | Teng S.-S.,Zhejiang Metallurgical Research Institute | And 11 more authors.
Conservation Genetics Resources | Year: 2014

In this study, 28 polymorphic simple sequence repeats (SSR) markers were isolated from an expressed sequence tag (EST) library of Meretrix lamarkii Deshayes. The number of alleles of these 28 EST-SSR markers varied from 2 to 8, with a mean of 3.64 alleles per locus. The observed, expected heterozygosity and polymorphic information content per locus varied from 0.10 to 1.00, 0.10 to 0.86 and 0.09 to 0.81, respectively. No significant linkage disequilibrium between pairs of loci was found, except two of them significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These loci are the first time to be characterized and will be useful for population genetic structure investigation and marker assisted breeding for M. lamarkii. © 2013, Springer Science+Business Media Dordrecht. Source


Liu B.,Zhejiang Metallurgical Research Institute | Liu B.,Zhejiang Key Laboratory of Exploitation and Reservation of Coastat Bio Resource | Teng S.-S.,Zhejiang Metallurgical Research Institute | Teng S.-S.,Zhejiang Key Laboratory of Exploitation and Reservation of Coastat Bio Resource | And 6 more authors.
Conservation Genetics Resources | Year: 2012

We isolated and characterized 39 expressed sequence tag (EST)-derived simple sequence repeats (SSR) for the blood clam Tegillarca granosa. The number of alleles of the 39 EST-SSR markers varied from 2 to 8, with a mean of 4.0 alleles per locus. The number of observed, expected heterozygosity and polymorphic information content per locus varied from 0.200 to 1.000, from 0.549 to 0.841 and from 0.253 to 0.849, respectively. No significant deviations from Hardy-Weinberg equilibrium or linkage disequilibrium between pairs of loci were observed. These loci are firstly characterized and will be useful for investigation genetic population structure and molecule-assisted breeding for T. granosa. © 2011 Springer Science+Business Media B.V. Source

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