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Zhijian C.,U.S. Center for Disease Control and Prevention | Zhijian C.,Zhejiang University | Xiaoxue L.,Zhejiang University | Wei Z.,Zhejiang International Travel Healthcare Center | And 6 more authors.
Biochemical and Biophysical Research Communications | Year: 2013

In the present study, the protein microarray was used to investigate the protein expression in human B-cell lymphoblastoid cells intermittently exposed to 1.8-GHz GSM radiofrequency radiation (RFR) at the specific absorption rate (SAR) of 2.0. W/kg for 24. h. The differential expression of 27 proteins was found, which were related to DNA damage repair, apoptosis, oncogenesis, cell cycle and proliferation (ratio >1.5-fold, P<. 0.05). The results validated with Western blot assay indicated that the expression of RPA32 was significantly down-regulated (. P<. 0.05) while the expression of p73 was significantly up-regulated in RFR exposure group (. P<. 0.05). Because of the crucial roles of those proteins in DNA repair and cell apoptosis, the results of present investigation may explain the biological effects of RFR on DNA damage/repair and cell apoptosis. © 2013 Elsevier Inc.

Jiang K.,Zhejiang Institute of Quality Inspection Science | Lv Q.,Zhejiang International Travel Healthcare Center | Zhang D.,Zhejiang Institute of Quality Inspection Science | Gao X.,Tianjin University of Science and Technology | And 6 more authors.
Journal of Food, Agriculture and Environment | Year: 2012

For fast and efficient reasons, a multiplex loop-mediated isothermal amplification (mLAMP) to detect foodborne pathogens was studied. Three sets of LAMP primers for 3 kinds of pathogens were designed from nuc gene of S. aureus, ipaH gene of Shigella spp. and invA gene of Salmonella spp., respectively. The DNA(s) were specifically amplified in the presence of the templates from three foodborne pathogens in a single tube. The sensitivities of the mLAMP method were shown 1000 times higher in average on the detection for the genes than those of the classical PCR methods. The sequences of mLAMP assay-positive pathogen products were 99% fidelity with the individual pathogen DNA sequence by sequencing. The large number of tests with field samples showed that the specificity of the LAMP assay was high, and no cross-reaction of the primers and the templates occurred in the same tube. The mLAMP method is further improved and simplified in the detection of the three foodborne pathogens with great specificity and sensitivity.

Sun Y.,U.S. Center for Disease Control and Prevention | Yan J.,U.S. Center for Disease Control and Prevention | Mao H.,U.S. Center for Disease Control and Prevention | Zhang L.,U.S. Center for Disease Control and Prevention | And 5 more authors.
PLoS ONE | Year: 2013

Background: Chikungunya (CHIK) virus is a mosquito-borne emerging pathogen presenting great health challenges worldwide, particularly in tropical zones. Here we report a newly detected strain of CHIK, Zhejiang/chik-sy/2012, in China, a nonindigenous region for CHIK, using a modified approach based on the classic cDNA-AFLP. We then performed etiological and phylogenetic analyses to better understand its molecular characterization and phylogenetic pattern, and also to aid in further evaluating its persistence in Southeast Asia. Methods: By using this modified procedure, we determined for the first time the complete genome sequence of the chikungunya virus strain, Zhejiang/chik-sy/2012, isolated in 2012 from a patient in Zhejiang, China. Sequence analyses revealed that this positive single strand of RNA is 12,017 bp long. We found no single amino acid mutation in A226V, D284E and A316V. Phylogenetic analysis showed that our strain shared the greatest homology with a strain isolated in Taiwan, which was derived from a strain from Indonesia. Chik-sy/2012 is in a different clade from other CHIK viruses found in China previously. Conclusions: A modified cDNA-AFLP in virus discovery was used to isolate the first CHIK and the first complete genome sequence of virus strain chik-sy/2012 in 2012 from a patient with CHIK fever in Zhejiang, China. The infection displayed great phylogenetic distance from viruses detected in Guangdong, China, in 2008 and 2010, since they were derived from another evolutionary lineage. Additional molecular epidemiology data are needed to further understand, monitor and evaluate CHIK in China. © 2013 Sun et al.

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