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Wu Z.G.,Zhejiang Institute of the Subtropical Crops | Li X.X.,Zhejiang Institute of the Subtropical Crops | Li X.X.,Wenzhou Medical College | Lin X.C.,Zhejiang Agriculture And forestry University | And 7 more authors.
Genetic Resources and Crop Evolution | Year: 2014

Yam (Dioscorea spp.) is widely cultivated in China and many landraces are maintained by local farmers. However, there is little information available about their diversity and species identity. In this study, inter simple sequence repeat (ISSR) and sequence related amplified polymorphism (SRAP) techniques were used to assess genetic diversity within 21 yam landraces from seven cultivated populations. We observed high level of polymorphism among these landraces, specifically, 95.3 % for ISSR and 93.5 % for SRAP. Analysis of molecular variance revealed a significantly greater variation among the four yam species (40.39 %) and their populations (35.78 %) than within the populations (23.83 %). The unweighted pair group method arithmetic averages clusters and principal component analysis for 21 landraces formed four well-separated groups containing landraces of each of the four species, namely, Dioscorea opposita Thunb., Dioscorea alata L., Dioscorea persimilis Prain et Burkill, and Dioscorea fordii Prain et Burkill. The ISSR and SRAP primers were highly discriminatory among the 21 landraces; all 21 landraces could be easily differentiated using these primers. The average mean of gene flow (Nm = 0.1081) estimated from high genetic differentiation (Gst = 0.8222) suggested that gene flow among the populations was relatively restricted. The lack of genetic diversity within individual yam species suggests that it is critical to develop long-term strategies for enhancing genetic diversity within various yam species. © 2014 Springer Science+Business Media Dordrecht.

Wu Z.G.,Zhejiang Institute of the Subtropical Crops
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To reveal the effects of ecological factors on geo-herbalism,by researching the correlation between yields or qualities of Curcuma wenyujin and ecological factors. All measured dates were divided into three components, meteorological factors, soil conditions, the yields and qualities of geo-herbals, and analyzed through canonical correlation using SPSS software. The results showed that there were two canonical correlation models to explain correlation between meteorological factors and yields or qualities of medicinal materials, as well as soil conditions and yields or qualities, and both of canonical correlations reached the significant or extremely significant level. The analysis indicated that main ecological factors affecting the yields or qualities of medicinal materials were average temperature from May to June,the days of high temperature (over 35 degrees C) and average rainfall from August to October, annual average rainfall, altitude, available potassium content in soil. Conclusions:This study has tentatively made clear which ecological factors to affect the yields and qualities of Curcuma wenyujin; and provided some basis for revealing geo-herbalism of Curcuma wenyujin.

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