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Yang S.,Zhejiang University of Technology | Zhang H.,Zhejiang Institute of Quality Inspection Science
Food and Nutrition Research | Year: 2016

Background: Distiller's dried grains with solubles (DDGS) and soybean meal were used as the substrates for the production of polyunsaturated fatty acids (PUFA) in solid-state fermentation (SSF) by Mortierella alpine. These fermented products were fed to laying hens. PUFA enrichment from chicken breasts was studied. Methods: The maximum productivity of PUFA was achieved under optimized process condition, including 1% w/w yeast extract as additive, an incubation period of 5 days at 12°C, 10% v/w inoculum level, 75% moisture content, and pH 6.0. The hens were then fed with ration containing soybean DDGS, rapeseed oil, soybean oil, and peanut oil. The control group was fed with basal ration. Results: Under the optimal condition, M. alpine produced total fatty acids (TFA) of 182.34 mg/g dry substrate. It has better mycelial growth when soybean meal was added to DDGS (SDDGS). PUFA in fermentation product increased with higher soybean meal content. The addition of 70% soybean meal to DDGS substrate yielded 175.16 mg of TFA, including 2.49 mg eicosapentaenoic acid (EPA) and 5.26 mg docosahexaenoic acid (DHA). The ratios of ω-6/ω-3 found in chicken breasts fat were all lower than that found in control by 36.98, 31.51, 18.15, and 12.63% for SDDGS, rapeseed oil, soybean oil, and peanut oil, respectively. Conclusions: This study identified an optimized SSF process to maximize PUFA productivity by M. alpine as the strain. This PUFA-enriched feed increased the PUFA contents as well as the proportions of ω-6 and ω-3 in chicken breasts and liver. © 2016 Shengli Yang and Hui Zhang.


Zhu Y.,Zhejiang Institute of Quality Inspection Science | Fei T.,Jilin University | Ma Y.,South China University of Technology
ChemPlusChem | Year: 2016

Five 3,5-difluorophenyl-substituted ruthenium complexes [Ru(bpy)2(dfpbpy)(ClO4)2] (Ru-Fbpy-bpy; bpy=bipyridine, dfpbpy=5,5′-di(3,5-difluorophenyl)-2,2′-bipyridine), [Ru(dpp)2(dfpbpy)(ClO4)2] (Ru-Fbpy-dpp; dpp=4,7-diphenyl-1,10-phenanthroline), [Ru(dpp)2(dfpphen)(ClO4)2] (Ru-Fphen-dpp; dfpphen=5,5′-di(3,5-difluorophenyl)-1,10-phenanthroline), [Ru(dpp)2(4,7-dfpphen)(ClO4)2] (Ru-Fdpp-dpp; 4,7-dfpphen=4,7-di(3,5-difluorophenyl)-1,10-phenanthroline), and [Ru(4,7-dfpphen)3(AsF6)2] (Ru-Fdpp) were synthesized, and their photophysical, electrochemical, and electroluminescent properties were studied systematically. The introduction of the electron-withdrawing group 3,5-difluorophenyl leads to a redshift of the emission of the ruthenium complexes. In addition, the 3,5-difluorophenyl substituent extends the π conjugation of the ligand, and thus facilitates the metal-to-ligand charge-transfer process of the complexes. All the complexes display orange-red phosphorescent emissions centered at 638, 638, 624, 614, and 605 nm, respectively. Density functional theory calculations show that the lowest unoccupied molecular orbital of Ru-Fbpy-dpp, Ru-Fphen-dpp, and Ru-Fdpp-dpp are all distributed on the 3,5-difluorophenyl-substituted ligand. By using these complexes as emitters, highly luminescent single-layer devices are obtained. The optimized device based on Ru-Fdpp exhibits the highest luminous efficiency and power efficiency of 4.19 cd A-1 and 1.46 lm W-1, respectively. Bright ideas: Five 3,5-difluorophenyl-substituted ruthenium complexes have been synthesized and characterized. The photoluminescent efficiencies of the complexes improved after introduction of an electron-withdrawing substituent (see scheme). The complexes are suitable as dopant light-emitting materials for organic light-emitting diodes. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Zhu Y.,Zhejiang Institute of Quality Inspection Science | Ma Y.,Jilin University | Zhu J.,China North Vehicle Research Institute
Journal of Luminescence | Year: 2013

We present here the effect of counter anion size in tris(4,7-diphenyl-1,10- phenanthroline)ruthenium(II) complexes [Ru(dpp)3X2] (X=Cl-, BF4 -, ClO4 -, PF6 - and AsF6 -) on their doped electrophosphorescent device properties. The performance of organic light-emitting diodes (OLEDs) based on these complexes is influenced by the counter anions, that is the efficiency enhances by increasing the counter anion size from small Cl- to large AsF6 -. Among the five complexes, [Ru(dpp)3(AsF6)2] shows the best single layer diode performance with the luminous efficiency of 4.25 cd A-1. The possible reasons are carefully studied on the complex properties and their luminescence processes in light-emitting layers. It is found that the size of counter anion affects properties of complex in many aspects such as solubility and photophysical properties. The result shows that the differences in luminescence quantum yields of complexes, the energy-transfer abilities, as well as the charge injection, transfer and trapping abilities of devices related to the counter anions are the major factors in the different performance of doped OLEDs. © 2013 Elsevier B.V.


Xia D.-Z.,Zhejiang Chinese Medical University | Zhang P.-H.,Zhejiang Institute of Quality Inspection Science | Fu Y.,Zhejiang University | Yu W.-F.,Zhejiang Chinese Medical University | Ju M.-T.,Zhejiang Chinese Medical University
Food and Chemical Toxicology | Year: 2013

The protective effects of puerarin on liver damage were evaluated by carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. Male rats were orally treated with puerarin daily, and received CCl4 intraperitoneally twice a week for 4weeks. Our results showed that puerarin at doses of 50, 100, and 200mg/kgb.w. significantly reduced the elevated activities of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase at least 15%, 17%, 14% and 18%, respectively. In addition, puerarin at different doses significantly decreased (p<0.05) the level of hepatic thiobarbituric acid reactive substances compared to the CCl4-treated group. Furthermore, the treatment of puerarin was also found to significantly increase the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, and glutathione content at least 40%, 12%, 25%, 52%, 17% and 44% in the liver of CCl4-treated rats, respectively. Liver histopathology also showed that puerarin reduced the incidence of liver lesions induced by CCl4. The results suggest that puerarin exhibits potent hepatoprotective effects on CCl4-induced liver damages in rats, and that the hepatoprotective effects of puerarin may be due to both the inhibition of lipid peroxidation and to increase of antioxidant enzymes activity. © 2013 Elsevier Ltd.


Xia D.,Zhejiang Chinese Medical University | Fan Y.,Zhejiang Chinese Medical University | Zhang P.,Zhejiang Institute of Quality Inspection Science | Fu Y.,Zhejiang University | And 2 more authors.
Journal of Membrane Biology | Year: 2013

Hepatoprotective agents could prevent tissue damage and reduce morbidity and mortality rates; such agents may include folkloric or alternative treatments. The present study evaluated the protective effects of the flavonoid-rich fraction from rhizomes of Smilax glabra Roxb. (SGF) on carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. Sprague-Dawley male rats were orally treated with SGF daily and received CCl4 intraperitoneally twice a week for 4 weeks. Our results showed that SGF at doses of 100, 300 and 500 mg/kg significantly reduced the elevated activities of serum aminotransferases (ALT and AST), alkaline phosphatase and lactate dehydrogenase and the level of hepatic thiobarbituric acid-reactive substances compared to the CCl4-treated group. Moreover, SGF treatment was also found to significantly increase the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S- transferase and glutathione compared with CCl4-induced intoxicated liver. Histopathologic examination revealed that CCl4-induced hepatic damage was markedly reversed by SGF. The results suggest that SGF has hepatoprotective and antioxidant properties in CCl4-induced liver injury in rats. © 2013 Springer Science+Business Media New York.


Guo L.,Zhejiang Sci-Tech University | Ding H.,Zhejiang Institute of Quality Inspection Science
2014 17th International Conference on Electrical Machines and Systems, ICEMS 2014 | Year: 2015

A novel permanent magnet planar motor with moving multi-layer overlapping windings is presented in this paper. In the motor, overlapped X and Y windings are divided into four parts. By controlling the currents, normal forces and thrust forces along y and x axes can be adjusted individually to realize 5 degree-of-freedom(DOF) motion with thrust forces uncoupled. And the winding topology can weaken the influence of the end effects of coils without reducing winding utilization. By using surface current method and 3D FEM results, the flux density distribution and force characteristics of the motor are analysis. Based on the relationships between the main dimensions and the forces, the design criteria of the magnetic array and the windings are obtained. The experiments results of a prototype are in good agreement with the result from the analytical and FEM method. The validity of the analysis and simulation is verified. © 2014 IEEE.


Ding H.,Zhejiang Institute of Quality Inspection Science | Guo L.,Zhejiang Sci-Tech University
2014 17th International Conference on Electrical Machines and Systems, ICEMS 2014 | Year: 2015

Linear induction motors(LIM) with discontinuous secondary are more suitable for the application with sharp bend stroke than traditional continuous constructions. But the construction of segmental secondary narrows the eddy current loop area and aggravates the end effect. All these will result in increment of the eddy current loop resistance, decrement of thrust force and ripples in the forces. In order to overcome the disadvantages, the model of the LIM with discontinuous secondary is established by using the field-circuit combined analysis method. Based on the verification of the accuracy of the model by the experiment results of a LIM prototype, the influences of the secondary dimensions on the dynamic characteristics of the LIM were analyzed. And according to the periodicity of the ripples generated by the segments, the design criteria for the secondary are proposed, which is proved to be effective to eliminate the force ripples by simulation results. © 2014 IEEE.


Guo L.,Zhejiang Sci-Tech University | Ding H.,Zhejiang Institute of Quality Inspection Science
Applied Mechanics and Materials | Year: 2013

A novel 2-D permanent magnet array for a planar actuator was proposed, in which the portion between adjacent magnetic poles was divided to four small rectangular solids. Magnetic fields of the permanent magnet array were analyzed systematically by 3D finite element method. The analysis results show that, compared with the well-known Halbach magnet array, the proposed array has higher permanent magnet utilization. Keeping the whole permanent magnet volume constant, the arrays with several improved structures were put forward. The magnetic field analyze results prove that the utilization is best, when the ratio between the height of the magnetic poles magnetized vertically and the height of the portion magnetized horizontally is about 2. © (2013) Trans Tech Publications, Switzerland.


Xu S.,China Jiliang University | Zhang Y.,China Jiliang University | Jiang K.,Zhejiang Institute of Quality Inspection Science
Food and Function | Year: 2016

In this study, five different kinds of polysaccharides (AAP1, AAP2, AAP3, AAP4, and AAP5) were extracted from different varieties of Auricularia auricula through an alkali extraction process. Furthermore, the crude polysaccharides were deproteinized by the Sevag method. Auricularia auricula produced in the Shanxi province had the highest content of polysaccharide, 53.02%. The monosaccharide composition was determined by the GC method. Their antioxidant capacities in vitro were assessed by radical-scavenging capacity (DPPH, superoxide, and hydroxyl radicals), metal chelating ability and reducing-power methods. In addition, the evaluation of their antioxidant effects in vivo was performed using the C. elegans model. The yield of crude polysaccharides, monosaccharide composition and antioxidant activity of Auricularia auricula polysaccharides (AAPs) were different among samples from various sources. Among them, the strongest antioxidant activity was shown for AAP1, consisting of arabinose, xylose, 2-deoxy-d-glucose, mannose, glucose, and N-acetyl-d-glucosamine with the molar ratio of 1 : 0.44 : 0.33 : 1.67 : 1 : 0.17. It could scavenge free radicals, up-regulate stress-resistance-related enzymes including superoxide dismutase (SOD) by 70.04 ± 8.75% and CAT by 117.32 ± 8.06% and reduce the level of reactive oxygen species (ROS) in C. elegans under oxidative stress. The present results suggested that variety was an important factor that affects the antioxidant activity of A. auricula polysaccharides. © 2016 The Royal Society of Chemistry.


Han Y.G.,Zhejiang Institute of Quality Inspection Science | Chen H.B.,Zhejiang Institute of Quality Inspection Science
Applied Mechanics and Materials | Year: 2013

Transmittance is the key factor to the quality of solar glass. At present visible light transmittance (380-780 nm) and solar direct transmittance (300-2500 nm) were used to evaluate the light transmission property without considering the specificity of solar glass. In this work a new parameter of effective transmittance in the 380-1200 nm region which was consistent with the spectral response range of solar cells was proposed and calculated to evaluate the property. The value of effective transmittance lies between that of visible light transmittance and solar direct transmittance. Using effective transmittance instead could reflect the performance of solar glass on real applications properly. © (2013) Trans Tech Publications, Switzerland.

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