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He P.-F.,Zhejiang University of Technology | He L.,Zhejiang Forestry Academy | Zhang A.-Q.,Zhejiang University of Technology | Wang X.-L.,Hangzhou Baishanzu Biotech Co. | And 2 more authors.
Carbohydrate Polymers | Year: 2017

A water-soluble heteropolysaccharide (PUP60W-1) was purified from the hot water extract of sclerotia of P. umbellatus by chromatography with DEAE Sepharose Fast Flow and Sephacryl S200 High-Resolution. The primary structure of PUP60W-1 was elucidated by GC, GC–MS and NMR. PUP60W-1 was identified as a highly branched polysaccharide composed of fucose, glucose and galactose in a ratio of 1.0:0.9:13.3. The main repeating unit was identified as α-(1 → 6)-D-galactopyranan backbone with substitution of terminal α-galactopyranosyl residues at O-2 for two out of every three main chain galactose residues. Its chain conformation was studied by atom force microscopy and size exclusion chromatography coupled with multiple detectors. The results revealed that PUP60W-1 had a molecular weight of 2.47 × 104 Da with a polydispersity index of 1.04, and existed in water as compact sphere structures which could be disrupted into smaller spherical chain blocks after dispersion with sodium dodecyl sulfate. © 2016 Elsevier Ltd


Wang J.-F.,CAS Kunming Institute of Botany | Wang J.-F.,Zhejiang Forestry Academy | Gong X.,CAS Kunming Institute of Botany | Chiang Y.-C.,National Sun Yat - sen University | Kuroda C.,Rikkyo University
Journal of Biogeography | Year: 2013

Aim: The objectives of this study were to elucidate the phylogenetic origins and phylogeographical history of Ligularia hodgsonii, which exhibits a disjunct distribution between south-western-central China and Japan. Location: China and Japan. Methods: Three chloroplast DNA (cpDNA) spacer regions (trnQ-5′rps16, trnL-rpl32 and psbA-trnH) were sequenced in 280 individuals of L. hodgsonii isolated from 29 natural populations, including 23 from China and 6 from Japan. Phylogenetic inference was performed using MrBayes and beast analyses. Statistical dispersal-vicariance analysis (S-DIVA) was utilized to resolve the biogeographical events in L. hodgsonii, and to clarify the origin of the species' disjunct distribution. Results: Genetic variation in cpDNA revealed 19 unique haplotypes among populations. A high degree of genetic diversity (HT = 0.913) and a significant level of differentiation (GST = 0.933, NST = 0.989) were detected. Different haplotypes from continental and island populations were analysed, and the degree of genetic diversity observed within each of the two regions was similar. S-DIVA analysis supported the occurrence of a vicariance event between the continental and island areas. Main conclusions: Based on S-DIVA analysis, the non-overlapping cpDNA haplotypes and similar genetic diversity levels in continental and island populations, we conclude that the disjunct distribution of L. hodgsonii is the result of vicariance. Molecular dating suggests that the separation between populations on the islands of Japan (northern Honshu and Hokkaido) and mainland Asia occurred during the middle to late Pleistocene. Following divergence, L. hodgsonii populations probably underwent severe range contraction into multiple isolated refugia during the Last Glacial Maximum, when conditions were colder and drier than at present. © 2013 Blackwell Publishing Ltd.


Zhang X.,Zhejiang Agriculture And forestry University | Shen A.,Zhejiang Forestry Academy | Wang Q.,Zhejiang Agriculture And forestry University | Chen Y.,Zhejiang Forestry Academy
African Journal of Biotechnology | Year: 2012

Fourteen symbiotic isolates were obtained from root nodules of Casuarina equisetifolia and Casuarina cunninghamiana in Zhejiang, China. All isolates exhibited typical Frankia morphological characteristics, including filamentous hyphae, vesicles, and multilocular sporangia borne terminally or in an intercalary position. Combined with 16S rDNA sequence alignment results, all isolates were identified as Frankia spp. 4 isolates belonged to the physical Group A, 7 to Group B, and 3 to Group AB. The strains demonstrated varied nitrogenase activities, with the ZCN192 strain being the highest (2.897 μmol·mg -1h -1) and ZCN199 the lowest (0.056 μmol·mg -1h -1). After in vivo inoculation, all strains significantly increased seedling height, basal diameter, and dry biomass of Casuarina spp. Generally, strains with higher nitrogenase activities exhibited more effective nitrogen fixation in vivo. © 2012 Academic Journals.


Feng Y.-L.,Zhejiang University | Li W.-Q.,Zhejiang University | Wu X.-Q.,Zhejiang Forestry Academy | Cheng J.-W.,Zhejiang Forestry Academy | Ma S.-Y.,Zhejiang University
Biochemical Engineering Journal | Year: 2010

Lentinus edodes (shiitake mushroom) is a well-known medicinal and edible mushroom throughout the world. Statistical optimization was employed to optimize the culture medium for maximum mycelial growth and exo-polysaccharide (EPS) production in submerged fermentation: Plackett-Burman design (PBD) was applied to determine significant factors, followed by the paths of steepest ascent to move to the general vicinity of the optimum and Box-Behnken design (BBD) to obtain the final optimum culture medium composition. Glucose, yeast-powder and pH were significant for fermentation. 15.4 g glucose and 5.32 g yeast-powder per liter and pH 4.61 were optimum for the biomass accumulation from 2.75 g/l to 6.88 g/l, while 15.78 g glucose and 5.86 g yeast-powder per liter and pH 4.48 were optimum for EPS production from 0.214 g/l to 0.751 g/l. Under the optimized cultivation condition, cultivation kinetic models were studied in flask (pellet growth form) and airlift (filamentous growth form) reactors. The two morphologies resulted in different culture processes and mathematical models. © 2009 Elsevier B.V. All rights reserved.


Feng Y.,Zhejiang University | Li W.,Zhejiang University | Wu X.,Zhejiang Forestry Academy | He L.,Zhejiang Forestry Academy | Ma S.,Zhejiang University
Carbohydrate Polymers | Year: 2010

Lentinan (LNT) is a highly functional biomacromolecule, and its sulfated derivatives (sLNT) exhibit improved pharmacological properties compared to LNT. In the present study, LNT extracted from the fermentation broth of Lentinus edodes was sulfated using two methods, conventional heating and novel microwave radiation, and their biological activities were evaluated using an antioxidant system and an antiproliferation cell model. Our results indicate that the two sulfated derivates have similar physicochemical properties and spectroscopic profiles. Nevertheless, microwave radiation produced a higher yield of sLNT, with a shorter processing time and lower degradation. In contrast to LNT, the sLNT derivatives exhibited a strong antioxidant activity in vitro. In addition, the derivatives exhibited a significant antiproliferative effect on leukemia cells, whereas LNT exhibited the opposite outcome. © 2010 Elsevier Ltd. All rights reserved.


Li B.,Zhejiang Agriculture And forestry University | Li B.,Zhejiang Forestry Academy | Chen S.-W.,Zhejiang Forestry Academy | Chen Z.,Zhejiang Agriculture And forestry University | And 3 more authors.
CrystEngComm | Year: 2011

A very unusual two-dimensional metal-organic nanotubular structure, [CuCl2(DTB)2] (DTB = 1,3-di(1H-tetrazol-1-yl)benzene) constructed from CuCl2 chains and ditetrazoles is reported, which represents the first example of nanotubular metal-organic frameworks based on Cu-X (X = halogen) chains. © The Royal Society of Chemistry 2011.


Hong C.,Zhejiang University | HaiBo L.,Zhejiang Forestry Academy | YunFeng X.,Zhejiang University
Enzyme and Microbial Technology | Year: 2010

The effect of a static magnetic field on the acclimation of activated sludge with high polyhydroxyalkanoate (PHA) storage capacity was evaluated under aerobic dynamic feeding (ADF) conditions. The acclimation processes were carried out in Sequence Batch Reactors (SBRs) with static magnetic field intensities of 42, 21, 7, or 0mT. Static magnetic exposure significantly influenced the PHA accumulation in activated sludge. PHA content in biomass reached 66% (on a COD basis) at a magnetic field intensity of 21mT and an organic load of 5.42gL -1, which was 16% higher than that of the control. Analysis by PCR-DGGE showed that the underlying mechanism might involve a change in the dominant species of microorganism in each reactor in response to the static magnetic field. © 2010 Elsevier Inc.


Guo J.,Zhejiang Agriculture And forestry University | Chen S.,Zhejiang Forestry Academy | Liu L.,Zhejiang Agriculture And forestry University | Li B.,Zhejiang Agriculture And forestry University | And 3 more authors.
Journal of Colloid and Interface Science | Year: 2012

Dyeing wastewater removal is important for the water treatment, and adsorption is an efficient treatment process. In this study, three modified bentonites, chitosan modified bentonite (CTS-Bent), hexadecyl trimethyl ammonium bromide (CTAB) modified bentonite (CTAB-Bent), and both chitosan and hexadecyl trimethyl ammonium bromide modified bentonite (CTS-CTAB-Bent) were prepared and characterized by FTIR and XRD analysis. Batch experiments were conducted to evaluate the adsorptive removal of weak acid scarlet from aqueous phase using modified bentonites under different conditions. The results show that the adsorption capacity of weak acid scarlet onto natural bentonite was low (4.9%), but higher for 1CTS-Bent and 1CTS-10CTAB-Bent. The optimal conditions for weak acid scarlet adsorption were 1% chitosan, 10% CTAB, at 80°C and reaction time 2.5h. The best removal efficiency was ∼85%, and the adsorption capacity of weak acid scarlet was around 102.0mgg -1, much higher than that of commercial activated carbon (27.2mgg -1). These results suggest that 1CTS-10CTAB-Bent is an excellent adsorbent for effective weak acid scarlet removal from water. The adsorption isotherms of weak acid scarlet were investigated. It was found that Langmuir and Temkin models fitted the data very well (R 2>0.99). © 2012 Elsevier Inc.


Zhang Q.,Zhejiang University | Xu L.,Zhejiang University | Tang J.,Zhejiang University | Bai M.,Zhejiang Forestry Academy | Chen X.,Zhejiang University
Mycorrhiza | Year: 2011

The biomass-density relationship (whereby the biomass of individual plants decreases as plant density increases) has generally been explained by competition for resources. Arbuscular mycorrhizal fungi (AMF) are able to affect plant interactions by mediating resource utilization, but whether this AMF-mediated interaction will change the biomass-density relationship is unclear. We conducted an experiment to test the hypothesis that AMF will shift the biomass-density relationship by affecting intraspecific competition. Four population densities (10, 100, 1,000, or 10,000 seedlings per square meter) of Medicago sativa L. were planted in field plots. Water application (1,435 or 327. 7 mm/year) simulated precipitation in wet areas (sufficient water) and arid areas (insufficient water). The fungicide benomyl was applied to suppress AMF in some plots ("low-AMF" treatment) and not in others ("high-AMF" treatment). The effect of the AMF treatment on the biomass-density relationship depended on water conditions. High AMF enhanced the decrease of individual biomass with increasing density (the biomass-density line had a steeper slope) when water was sufficient but not when water was insufficient. AMF treatment did not affect plant survival rate or population size but did affect absolute competition intensity (ACI). When water was sufficient, ACI was significantly higher in the high-AMF treatment than in the low-AMF treatment, but ACI was unaffected by AMF treatment when water was insufficient. Our results suggest that AMF status did not impact survival rate and population size but did shift the biomass-density relationship via effects on intraspecific competition. This effect of AMF on the biomass-density relationship depended on the availability of water. © 2010 Springer-Verlag.


Jia J.,CAS Kunming Institute of Botany | Jia J.,University of Chinese Academy of Sciences | Wu H.,CAS Kunming Institute of Botany | Wang J.-F.,Zhejiang Forestry Academy | Gong X.,CAS Kunming Institute of Botany
Genetic Resources and Crop Evolution | Year: 2014

Munronia delavayi Franch. (Meliaceae) is a vulnerable perennial species that is narrowly distributed and endemic to the dry-hot valley of the middle/lower Jinsha River. However, 12 hydropower stations are currently scheduled to be built in the middle and lower Jinsha River drainage and their distribution overlaps with the range of M. delavayi. The construction of these hydropower stations will cause flooding of the adjacent habitats that support M. delavayi and will change the local ecological environment, which may result in a new and dangerous situation for the survival of this species. Our aim was to evaluate the population structure and propose the most suitable conservation strategy for M. delavayi to support its adaptive potential. In this study, we collected 70 individuals from seven populations that covered most of the geographic range along the Jinsha River and investigated the population diversity, genetic structure and demographic history of this species by analyzing the variations in one mitochondrial marker (18sf-5sr), two non-coding chloroplast DNA regions (trnT-trnL, trnS-trnG), and one nuclear DNA marker (Gs687f-994r). As a result, three mitotypes, nine chlorotypes and six haplotypes with high genetic diversity (HT = 0.580, 0.774 and 0.740) were detected based on mtDNA, cpDNA and nDNA and a strong genetic structure (FST = 0.855 and 0.545) was detected based on cpDNA and nDNA. Our study also indicated that habitat fragmentation and limited gene flow may result in the genetic differentiation of this species and that the population distributed in Qiaojia, Yongshan and Panzhihua should be proactively protected based on the conservation genetic analysis. © 2014, Springer Science+Business Media Dordrecht.

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