Zhejiang California International Nanosystems Institute ZCNI

Hangzhou, China

Zhejiang California International Nanosystems Institute ZCNI

Hangzhou, China

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Mou X.,Zhejiang University | Mou X.,Zhejiang California International Nanosystems Institute ZCNI | Wan S.,Zhejiang University | Li Y.,Zhejiang University | And 10 more authors.
PLoS ONE | Year: 2011

The interaction between mammalian host cells and bacteria is a dynamic process, and the underlying pathologic mechanisms are poorly characterized. Limited information describing the host-bacterial interaction is based mainly on studies using label-based endpoint assays that detect changes in cell behavior at a given time point, yielding incomplete information. In this paper, a novel, label-free, real-time cell-detection system based on electronic impedance sensor technology was adapted to dynamically monitor the entire process of intestinal epithelial cells response to Salmonella infection. Changes in cell morphology and attachment were quantitatively and continuously recorded following infection. The resulting impedance-based time-dependent cell response profiles (TCRPs) were compared to standard assays and showed good correlation and sensitivity. Biochemical assays further suggested that TCRPs were correlated with cytoskeleton-associated morphological dynamics, which can be largely attenuated by inhibitions of actin and microtubule polymerization. Collectively, our data indicate that cell-electrode impedance measurements not only provide a novel, real-time, label-free method for investigating bacterial infection but also help advance our understanding of host responses in a more physiological and continuous manner that is beyond the scope of current endpoint assays. © 2011 Mou et al.


Liu F.,Zhejiang University | Mou X.,Zhejiang University | Mou X.,Zhejiang California International Nanosystems Institute ZCNI | Zhao N.,Zhejiang University | And 6 more authors.
Colorectal Disease | Year: 2011

Aim The prevalence of human papillomavirus (HPV) was determined in Chinese patients with colorectal cancer (CRC). The study also aimed to determine whether the HPV DNA peripheral blood (PB) assay can be used to diagnose HPV-related CRC. Method Tumour tissue, noncancerous colorectal tissue and whole-blood samples were obtained from 96 patients with CRC. In addition, 32 colorectal tissue samples were harvested from patients without CRC, and 48 whole-blood samples were collected from healthy blood donors. HPV DNA was detected by means of a nested polymerase chain reaction (PCR) using consensus primers, and HPV genotypes were determined by reverse Southern blot and pyrosequencing. Results HPV DNA was detected in 32 of the 96 patients with CRC, and colorectal tissues from the 32 control patients without CRC were negative for HPV DNA (P<0.001). Among 48 healthy donors, three had detectable levels of HPV DNA in their PB. Patients with CRC did not have significantly higher levels of HPV DNA than controls. The HPV prevalence in tumour tissues was higher than that in noncancerous colorectal tissues (P<0.001) or that in PB samples (P<0.001). No correlation between the presence of HPV and demographic or medical characteristics was observed. HPV 16 was the viral type most frequently detected and was found in 33 (94%) of 35 HPV-positive patients. Conclusion HPV infection may be a risk factor for CRC. However, detection of HPV DNA in PB does not appear to reflect the HPV status of CRC. © 2011 The Authors. Colorectal Disease © 2011 The Association of Coloproctology of Great Britain and Ireland.


Mou X.,Zhejiang University | Mou X.,Zhejiang California International Nanosystems Institute ZCNI | Chen L.,Zhejiang University | Liu F.,Zhejiang University | And 7 more authors.
PLoS ONE | Year: 2012

Background: JCV is a DNA polyomavirus very well adapted to humans. Although JCV DNA has been detected in colorectal cancers (CRC), the association between JCV and CRC remains controversial. In China, the presence of JCV infection in CRC patients has not been reported. Here, we investigated JCV infection and viral DNA load in Chinese CRC patients and to determine whether the JCV DNA in peripheral blood (PB) can be used as a diagnostic marker for JCV-related CRC. Methodology/Principal Findings: Tumor tissues, non-cancerous tumor-adjacent tissues and PB samples were collected from 137 CRC patients. In addition, 80 normal colorectal tissue samples from patients without CRC and PB samples from 100 healthy volunteers were also harvested as controls. JCV DNA was detected by nested PCR and glass slide-based dot blotting. Viral DNA load of positive samples were determined by quantitative real-time PCR. JCV DNA was detected in 40.9% (56/137) of CRC tissues at a viral load of 49.1 to 10.3×104 copies/μg DNA. Thirty-four (24.5%) non-cancerous colorectal tissues (192.9 to 4.4×103 copies/μg DNA) and 25 (18.2%) PB samples (81.3 to 4.9×103 copies/μg DNA) from CRC patients were positive for JCV. Tumor tissues had higher levels of JCV than non-cancerous tissues (P = 0.003) or PB samples (P<0.001). No correlation between the presence of JCV and demographic or medical characteristics was observed. The JCV prevalence in PB samples was significantly associated with the JCV status in tissue samples (P<0.001). Eleven (13.8%) normal colorectal tissues and seven (7.0%) PB samples from healthy donors were positive for JCV. Conclusions/Significance: JCV infection is frequently present in colorectal tumor tissues of CRC patients. Although the association between JCV presence in PB samples and JCV status in tissue samples was identified in this study, whether PB JCV detection can serve as a marker for JCV status of CRC requires further study. © 2012 Mou et al.


Chen L.,Zhejiang University | Mou X.,Zhejiang University | Mou X.,Zhejiang California International Nanosystems Institute ZCNI | Zhang Q.,Zhejiang University | And 8 more authors.
Molecular Medicine Reports | Year: 2012

The aims of the present study were to investigate the genetic characteristics of enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) strains in China and to evaluate the relationship between the genotypes of CVA16 and EV71 and their geographical distribution. A total of 399 stool specimens were collected from children with symptoms of hand, foot and mouth disease (HFMD) in Zhejiang Province. The presence of enteroviruses was determined using reverse transcription-semi-nested PCR targeted to the VP1 gene of all human enteroviruses and DNA sequencing. EV71 and CVA16, the major etiological agents of HFMD, were detected in 38.4% (38/99) and 35.4% (35/99) of HEV-A species-positive cases, respectively. Based on the phylogenetic analysis of the VP1 gene, EV71 strains identified in this study belong to subgenotype C4, and CVA16 strains herein were classified into clusters B2a and B2b within the genotype B2. Taking into consideration other published data, we conclude that the genetic characteristics of enteroviruses in China reflect the pattern of the endemic circulation of the subgenotype C4 to EV71 and clusters B2a and B2b within genotype B2 to CVA16, which have been continuously circulating in China since 1997. This observation indicates that the genetic characteristics of enteroviruses in China seem to depend on their special geographical and climatical features allowing them to be sustained with little external effect.


Mou X.,Zhejiang University | Mou X.,Institute of Molecular Pathology and Molecular Cell Biology | Mou X.,Zhejiang California International Nanosystems Institute ZCNI | Chen L.,Zhejiang University | And 10 more authors.
Journal of International Medical Research | Year: 2011

This retrospective study investigated the presence of human papillomavirus (HPV) in Chinese women with breast cancer, and the correlation between HPV infection and carcinogenesis. Tumour and noncancerous breast tissue samples were obtained from 62 female patients with breast cancer; normal breast tissue samples were obtained from 46 women without breast cancer. HPV DNA was detected by nested polymerase chain reaction using consensus primers; HPV subtypes were determined by reverse dot blot and pyrosequencing analyses. HPV was found in tumour tissue samples from four of the 62 patients (6.5%), while no HPV DNA was detected in either the noncancerous samples from patients with breast cancer or from the normal breast tissue controls. Of the four HPV-positive cases, three were HPV 16 positive (75%) and one was HPV 18 positive (25%). The low frequency of HPV detected in this study suggests that this infection is not a major risk factor in breast cancer development. © 2011 Field House Publishing LLP.

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