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Tang Q.,Zhejiang California International NanoSystems Institute
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences

To investigate the effect of benzo(a)pyrene (BaP) on platelet aggregation and expression of P-selectin. Blood samples were collected from healthy volunteers and the platelets was washed. Platelet aggregation was monitored by aggregometer and the expression of P-seletin was detected by whole blood flow cytometry. BaP (10 μmol/L, 1 μmol/L and 0.1 μmol/L) did not induce platelet aggregation; however, preincubation with BaP (10 μmol/L) significantly enhanced ADP-induced platelet aggregation (P < 0.01) and platelet aggregation was (80 ± 10)%, while BaP-preincubation failed to enhance platelet aggregation under collagen and thrombin stimulation. Flow cytometry showed that preincubation with BaP increased ADP-induced, but not thrombin-induced P-selectin expression (P < 0.01). BaP can stimulate ADP-induced platelet aggregation and P-selectin expression, probably through the interaction with ADP-mediated signal pathway. Source

Liu X.,Zhejiang University | Jiang J.,Zhejiang University | Shao J.,Zhejiang California International NanoSystems Institute | Yin Y.,Zhejiang University | Ma Z.,Zhejiang University
Applied Microbiology and Biotechnology

Using a deep serial analysis of gene expression (DeepSAGE) sequencing approach, we profiled the transcriptional response of Fusarium graminearum to tebuconazole, a most widely used azole fungicide. By comparing the expression of genes in F. graminearum treated and untreated with tebuconazole, we identified 324 and 155 genes showing more than a 5-fold increase and decrease, respectively, in expression upon tebuconazole treatment. These genes are involved in a variety of cell functions including egrosterol biosynthesis, transcription, and cellular metabolism. The validity of DeepSAGE results were confirmed by real-time PCR analysis of expression of 20 genes with different expression levels in the DeepSAGE analysis. The results from this study provide useful information in understanding the mechanisms for the responses of F. graminearum to azole fungicides. © 2009 Springer-Verlag. Source

Yan C.,Zhejiang University | Wu W.,Zhejiang University | Li H.,Zhejiang University | Zhang G.,Zhejiang University | And 4 more authors.
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Benzo[a]pyrene (BaP) is a potent pro-carcinogen generated from the combustion of fossil fuel and cigarette smoke. Previously, using a proteomic approach, we have shown that BaP can induce changes in the expression of many cellular proteins, including transcription regulators. In the present study, using a similar approach, we examined the nuclear protein response to BaP in HeLa cells and found that BaP treatment caused expression changes in many nuclear proteins. Twenty-four of these proteins were successfully identified, several of which are involved in the alternative splicing of mRNA, DNA replication, recombination, and repair. The changed expression levels were further confirmed by immunoblot analysis using specific antibodies for two proteins, Lamin A and mitotic checkpoint protein Bub3. The nuclear localization of these two proteins was also confirmed by confocal microscopy. To determine whether alternative splicing was activated following BaP treatment, we examined Fas and CD44, two genes previously shown to be targets of alternative splicing in respond to DNA damage. While no significant activation of alternative splicing was observed for Fas, CD44 splicing variants were found after BaP treatment. Together, these data show that DNA damage induces dramatic changes in nuclear protein expression, and that alternative splicing might be involved in the cellular response to DNA damage. © 2010 Elsevier B.V. All rights reserved. Source

Tong J.,Jiaxing Glead Electronics Co. | Zhang B.,Jiaxing Glead Electronics Co. | Huang W.,Jiaxing Glead Electronics Co. | Yang H.,Zhejiang California International NanoSystems Institute
Materials Letters

The effect of composition of Li2(Mg0.3Zn 0.7)Ti3O8-xTiO2 (-0.36≤x≤0.36, for short LMZT) on sinterability, crystalline phase, microstructure and microwave dielectric properties of the ZnO-B2O3-SiO 2 (for short ZBS) glass doped samples was systematically investigated. It shows that as x is over zero, rutile phase was detected and the crystalline grain size of LMZT ceramics decrease abruptly. It was also found that the bulk density and the quality factor (Q×f) gradually decreased, dielectric constant (εr) increased, the temperature coefficient of resonant frequency (τf) shifted to positive value with the increase of x value. When x=0.12, LMZT ceramics with 3 wt% ZBS sintered at 900 °C for 3 h show excellent dielectric properties: εr=25.1, Q×f=19,620 GHz, τf=-2.3 ppm/°C. It is compatible with Ag electrodes, which makes it a promising ceramic for low temperature co-fired ceramics technology application. © 2013 Elsevier B.V. Source

Xin C.-R.,Zhejiang University | Zhang J.,Zhejiang University | Liu Y.,University of Sheffield | Zhang Q.-L.,Zhejiang University | And 3 more authors.
Materials Research Bulletin

Sc-doped barium titanate (BaTiO3) nanopowders and ceramics were prepared by a sol-gel method for the first time. The polymorphism, microstructure and dielectric properties were investigated. Pseudo-cubic Sc-doped BaTiO3 nanopowders with a particle size of 30-40 nm have been achieved at low temperature of 800 °C. A phase diagram for the solid solution system BaTi1-xScxO3-δ was obtained. The limit of pseudo-cubic Sc-doped BaTiO3 solid solution (BTss) and the stable hexagonal phase have a large dependence on temperatures. The dopant of Sc3+ can also dramatically refine the grain size of BaTiO3 to 300 nm when x = 0.10. For different Sc3+ content in BaTiO3 ceramics, two types of impedance response appeared, and the resistivity of ceramics was dominated by bulk resistance or grain boundary resistance. Curie temperature (TC) and dielectric constant decreased by increasing the Sc3+ content. Relaxor behaviour occurs below room temperature when x = 0.10. © 2013 Elsevier Ltd. Source

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