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Ge Y.,Beijing Forestry University | Ge Y.,Lishui Academy of Forestry Science | He X.,Lishui Academy of Forestry Science | Wang J.,Lishui Academy of Forestry Science | And 3 more authors.
Acta Physiologiae Plantarum | Year: 2014

Phoebe bournei commonly called nanmu is an important and endemic wood species in China, and its planting, nursing, and preserving are often affected by drought stress. Two-year-old P. bournei seedlings were subjected to water stress and recovery treatment to study their physiological and biochemical responses. Physiological and biochemical indices did not change when seedlings were subjected to mild water stress (<15 days of water withholding). As drought stress intensified (>20 days of water withholding), malondialdehyde and electrolyte leakage increased, and chlorophyll and soluble protein decreased, indicating an increased oxidative stress induced by water deficit. Enhanced activities of superoxide dismutase (SOD) and peroxidase (POX), accumulation of free proline and total soluble sugar contribute to plant protection against the oxidative stress. However, SOD and POX decreased when seedlings were subjected to an extended drought. After 5 days of recovery, physiological and biochemical indices were not restored to the control level values except for leaf relative water content when the seedlings were subjected to more than 20 days water stress. These results demonstrate that P. bournei could enhance their ability to mitigate water stress effects by up-regulating antioxidant system and osmotic adjustment, but these two protective mechanisms were limited when seedlings were subjected to moderate and severe water stress. The threshold of water deficit to P. bournei seedlings is 15-20 days, and permanent damage will be induced if water status is not improved before this threshold. The results will provide some theoretical and practical guidance for nanmu afforestation and production. © 2014 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków. Source

Ge Y.J.,Beijing Forestry University | Ge Y.J.,Lishui Academy of Forestry Science | Liu Y.J.,Lishui Academy of Forestry Science | Shen A.H.,Zhejiang Academy of Forestry science | Lin X.C.,Zhejiang Agriculture And forestry University
Genetics and Molecular Research | Year: 2015

Fengshui forests (sacred groves) are important in traditional Chinese culture and home to many endangered species. These forests may provide protection for some endangered plant species outside the nature reserves, but little is known about their role in genetic conservation. Using inter-simple sequence repeat (ISSR) markers, we compared the genetic diversity of 6 populations of Phoebe bournei (Hemsl.) Yang, a commercially important woody species, which is under second-class national protection and endemic to China. Samples were collected from the nature reserves and Fengshui forests in southern China. Herein, we show that Fengshui forest populations are capable of maintaining some level of genetic diversity. For nature reserve populations, the average NA and NE were 1.58 and 1.39, respectively; and for Fengshui forests, they were 1.39 and 1.12, respectively. For nature reserve populations, Nei’s gene diversity (H) and Shannon’s index (I) were 0.32 and 0.11, respectively; and for Fengshui forests, they were 0.22 and 0.07, respectively. We discuss the reasons for the genetic differences between populations of the Fengshui forests and nature reserves and propose conservation strategies for the Fengshui forest. © FUNPEC-RP. Source

Cheng J.-W.,Zhejiang Academy of Forestry science | Cheng J.-W.,Key Laboratory of Biological and Chemical Utilization of Forest Resources | Wang Y.-B.,Zhejiang Academy of Forestry science | Wang Y.-B.,Key Laboratory of Forest Food | And 8 more authors.
World Journal of Microbiology and Biotechnology | Year: 2012

Optimization of fermentation process for the production of intracellular polysaccharide (IPS) from the fungus Paecilomyces cicadae and the immuno-stimulating activity of IPS were carried out. The quantitative effects of initial pH, fermentation temperature and time on the yield of IPS content produced by P. cicadae in submerged fermentation were investigated separately using response surface methodology (RSM). The three factors chosen for the present investigation were based on the results of a previous Plackett-Burman (PB) design. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods. RSM analysis showed good correspondence between experimental and predicted values. It was found that three parameters represented significant effect. Probability value (p < 0.0001) demonstrated a very high significance for the regression model. By solving the regression equation and analyzing the response surface contour plots, the optimal process parameters were determined, i. e. fermentation temperature 24.53 °C, initial pH 7.46 and fermentation time 73.9 h. The maximum predicted yield of IPS was 356.02 μg/ml under the optimal conditions. Meanwhile, IPS from P. cicadae was found to have direct immuno-stimulating activity in vitro on murine macrophage RAW264.7 proliferative response and to stimulate nitric oxide generation in a dose-dependent manner. © 2012 Springer Science+Business Media B.V. Source

Fu L.-Z.,Zhejiang Academy of Forestry science | Fu L.-Z.,Lishui Edible Fungi Research and Development Center | Zhang H.-Y.,Hebei Normal University | Wu X.-Q.,Zhejiang Academy of Forestry science | And 7 more authors.
World Journal of Microbiology and Biotechnology | Year: 2010

Random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers were used to evaluate the genetic diversity among 23 elite Lentinula edodes strains in China. A total of 138, 77 and 144 bands were detected by 16 RAPD primers, 5 ISSR primers and 23 SRAP primer combinations, among which 58.8%, 73.5% and 56.3% was polymorphic, respectively. By UPGMA clustering, a dendrogram was constructed based on each analysis. The three dendrograms showed that 23 L. edodes strains were clustered into three or four groups. The grouping exhibited similar structure and was generally consistent with their pedigrees. Twenty-three L. edodes strains shared great similarity indicated that the low level of genetic diversity of L. edodes strains and their relationship between each other. The important source of breeding material, such as wild and exotic types, must be introduced in order to broaden genetic base and decreases genetic vulnerability of L. edodes. © 2009 Springer Science+Business Media B.V. Source

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