Zhe Jiang Academy of Medical Science

Hangzhou, China

Zhe Jiang Academy of Medical Science

Hangzhou, China
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Chen W.,Zhe Jiang Academy of Medical Science | Ruan Z.,Zhe Jiang Academy of Medical Science | Xu C.,Zhe Jiang Academy of Medical Science | Qian Y.,Zhe Jiang Academy of Medical Science
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2014

OBJECTIVE: To assess the value of blood N-methylcarbamoylated haemoglobin (NMHb) as a biomarker of N, N-dimethylformamide (DMF) exposure.METHODS: Seventy-two DMF processing workers in a synthetic leather factory were included in the DMF exposure group, and 12 workers in a food factory without exposure to DMF were included in the control group. Long-time individual sampling in workplace was performed among 45 workers in the exposure group, accompanied by a questionnaire survey. Blood and urine were collected for the determination of urinary N-methylformamide (NMF), urinary creatinine, and blood NMHb. Air DMF and urinary NMF were determined by gas chromatography. NMHb in blood was degraded to 3-methyl-5-isopropylhydantoin by Edman degradation before it could be determined by gas chromatography/mass spectrometry.RESULTS: Air DMF in workplace and NMF in post-shift urine were both correlated with NMHb in blood, and the respective regression equations were LgNMHb (nmol/g globin) = 0.32×LgDMF (mg/m(3))+1.8 (r = 0.60, P < 0.005), and LgNMHb (nmol/g globin) = 0.47×LgNMF (mg/g Cr) + 1.4 (r = 0.56, P < 0.005).CONCLUSION: NMHb can be used as a biomarker of long-term exposure to DMF.


Zhu H.,Zhejiang University | Zhu H.,Zhe Jiang Academy of medical science | Chen H.,Zhe Jiang Academy of medical science | Zhong Y.,Zhejiang University | And 4 more authors.
Journal of Chromatographic Science | Year: 2010

Ion chromatography has been proposed for the determination of three common inorganic anions (chloride, nitrate, and sulfate) in nine weak acids (tartaric acid, citric acid, formic acid, acetic acid, metacetonic acid, butyric acid, butanedioic acid, hexafluorophosphoric acid, and salicylic acid) using a single pump, two valves, a single eluent, and a single conductivity detector. The present system uses ion exclusion, concentrator, and anionexchange columns connected in series via 6-port and 10-port valves in a Dionex ICS-2100 ion chromatograph. The valves were switched for the determination of three inorganic anions from weak acids in a single chromatographic run. Sample matrices of weak acids with a series of concentrations can be investigated. Complete separations of the previously mentioned anions are demonstrated within 40 min. Under the optimum conditions, the relative standard deviation values ranged from 1.3 to 3.8%. The detection limits of the three inorganic anions (S/N = 3) were in the range of 0.3-1.7 μg/L. The recoveries were in the range of 75.2-117.6%. With this system, automation for routine analysis, short analysis time, and low cost can be achieved.


PubMed | Zhe Jiang Academy of Medical Science
Type: Journal Article | Journal: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2014

To assess the value of blood N-methylcarbamoylated haemoglobin (NMHb) as a biomarker of N, N-dimethylformamide (DMF) exposure.Seventy-two DMF processing workers in a synthetic leather factory were included in the DMF exposure group, and 12 workers in a food factory without exposure to DMF were included in the control group. Long-time individual sampling in workplace was performed among 45 workers in the exposure group, accompanied by a questionnaire survey. Blood and urine were collected for the determination of urinary N-methylformamide (NMF), urinary creatinine, and blood NMHb. Air DMF and urinary NMF were determined by gas chromatography. NMHb in blood was degraded to 3-methyl-5-isopropylhydantoin by Edman degradation before it could be determined by gas chromatography/mass spectrometry.Air DMF in workplace and NMF in post-shift urine were both correlated with NMHb in blood, and the respective regression equations were LgNMHb (nmol/g globin) = 0.32LgDMF (mg/m(3))+1.8 (r = 0.60, P < 0.005), and LgNMHb (nmol/g globin) = 0.47LgNMF (mg/g Cr) + 1.4 (r = 0.56, P < 0.005).NMHb can be used as a biomarker of long-term exposure to DMF.

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