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Xie J.,Fujian Medical University | Que W.,Fujian Medical University | Liu H.,Zhangzhou Health Vocational College | Liu M.,Second Hospital of Yulin | And 2 more authors.
Molecular Medicine Reports | Year: 2012

We extracted cucurmosin (CUS) from the sarcocarp of Cucrubita moschata (pumpkin). Recently, a number of studies have indicated that CUS has cytotoxic properties and induces apoptosis in a number of human tumor cells. However, the detailed mechanisms are largely unknown. The aim of this study was to confirm CUS's anticancer activity on human hepatoma HepG2 cells in vitro and in vivo, and to elucidate the mechanism of its activity. MTT was used to detect the cytotoxic effects of CUS. Flow cytometry was used to analyze cell apoptosis and the cell cycle. Transmission electron microscopy was used to observe the morphology of apoptotic cells. Western blot analysis was performed to measure the protein expression of bax, bcl-2 and procaspase-3. The established orthotopic transplantation models of human hepatoma in NOD/SCID mice were tested for anticancer activities in vivo. The results showed that CUS inhibited the proliferation of HepG2 cells in vitro and in vivo. CUS induced apoptosis and arrested the cell cycle. In addition, CUS increased the protein expression of bax, but decreased the bcl-2 and procaspase-3 expression in HepG2 cells. Our data indicate that CUS has potential anticancer activity for human hepatoma, which can be attributed in part to its inhibition of proliferation and apoptosis, induced by decreasing the bcl-2:bax ratio and caspase-3 activation.

Lin M.Z.,Zhangzhou Health Vocational College | Zhu Y.,Xiamen University | Yang S.J.,Xiamen University | Wei D.M.,Taizhou University | Tian H.Q.,Xiamen University
Flora: Morphology, Distribution, Functional Ecology of Plants | Year: 2014

We analyzed anther development in Amomum villosum Lour. (Zingiberaceae) using the periodic acid-Schiff's technique and Sudan black staining to test for the presence of starch and lipids, respectively. Our analyses showed that microspore mother cells of A. villosum lack typical callose walls, and numerous lipid granules appear in the cells early in development. Some starch granules are present in anther wall cells, but not in tapetal cells. After meiosis, numerous lipid granules remain unchanged in the microspores. During microspore development, some small starch granules first appear in the central cell region, and then the starch granules increase in size. After microspore division, the bicellular pollen grains become filled with starch and lipids, and remain in this state until the pollen grains reach maturity. At anthesis, the anther wall of A. villosum consists of several layers of endothecium cells with an evidently thickened radial wall, and some layers of parenchyma cells containing numerous starch granules. © 2014 Elsevier GmbH.

Gao B.,Huaqiao University | Luo X.,Zhangzhou Health Vocational College | Fu H.,Huaqiao University | Lin B.,Huaqiao University | And 2 more authors.
Materials Research Bulletin | Year: 2013

Nitrogen-doped Ti1-xZrxO2 solid solutions have been synthesized by a multi-step sol-gel process followed by hydrothermal treatment in ammonia solution. XRD, XPS and UV-vis diffuse reflectance analyses indicated that nitrogen was doped in the surface layer of catalyst, introducing surface states located close to valence band. However, Zr4+ was successfully incorporated into the bulk lattice of TiO2 so as to induce the up-shift of conduction band. Compared to pristine TiO2 and nitrogen-doped TiO2, nitrogen-doped Ti1-xZr xO2 exhibited much higher efficiency for the degradation of Acid Red 88 solution and gaseous benzene under visible light irradiation, attributed to the synergetic effect of nitrogen and zirconium on the energy band. Specifically, the presence of surface states in the band gap enabled the extended visible light response and the up-shift of conduction band facilitated the excited electron interfacial transfer and hence suppressed efficiently the recombination of charge carriers. © 2012 Elsevier Ltd.

Li Q.,Zhangzhou Health Vocational College | Weng K.,Fujian Medical University | Zhu L.,Fujian Medical University | Mei X.,Fujian Medical University | And 2 more authors.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2014

OBJECTIVE: To detect the percentage of total natural killer (NK) cells and its different populations in the peripheral blood from neonates with bacterial pneumonia and discuss the clinical significance of NK cells in the pathogenesis of bacterial pneumonia.METHODS: Flow cytometry was performed to detect the percentages of NK cells and its subsets in peripheral blood lymphocytes from 38 cases of neonatal bacterial pneumonias and 18 cases of normal neonates. Patients recruited were divided into two groups according to hospitalization days and numbers of peripheral leukocytes: hospitalization days within 10 days (including 10 days) as group A, and more than 10 days as group B; the number of peripheral blood leukocytes <5.0×10(9)/L or >20.0×10(9)/L as severe infection group, and 5.0×10(9)/L< number of peripheral blood leukocytes <20.0×10(9)/L as mild infection group.RESULTS: The percentages of peripheral blood NK cells and CD3(-)CD56(neg)CD16(bright) subset in the neonates with bacterial pneumonia were significantly lower than those of the normal newborns (P<0.01), but there were no statistically significant differences in CD3(-)CD56(bright)CD16(neg/dim) and CD3(-)CD56(dim)CD16(bright) subsets. The percentage of CD3(-)CD56(neg)CD16(bright) subset in group A was significantly lower than that of the normal newborns (P<0.01), while the percentages of the total NK cells and other subsets had no statistical significance. The neonates with bacterial pneumonia had significantly lower percentages of the total NK cells and CD3(-)CD56(neg)CD16(bright) subset in group B as compared with the normal neonates (P<0.01). And the percentages of the total NK cells and its subsets in group B were also lower than those in group A (P<0.05). The percentages of NK cells and each subset in severe infection group were significantly lower than those in mild infection group (P<0.05).CONCLUSION: To the neonates who suffer from bacterial pneumonia, the more serious and the longer hospital stay, the lower the percentages of NK cells and its subsets are.

Gao B.,Huaqiao University | Luo X.,Zhangzhou Health Vocational College | Fu H.,Huaqiao University | Chen Y.,Huaqiao University | And 2 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2014

Anatase TiO2 microspheres with a high percentage of exposed (001) facets were synthesized by a template-free hydrothermal method without using concentrated hydrofluoric acid. The influence of various experiment conditions, such as hydrothermal temperature, acid condition, reaction time, etc., on the morphology of the final products was investigated in detail. The formation mechanism of the microspheres was deduced according to the XRD, SEM, TEM, and HRTEM characterizations. The as-obtained microspheres exhibited superior photocatalytic activity in the degradation of Acid Red 88 compared to TiO2 without exposed (001) facet. Copyright © 2014 American Scientific Publishers.

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