Zhangzhou Health Vocational College

Zhangzhou, China

Zhangzhou Health Vocational College

Zhangzhou, China
Time filter
Source Type

Chen S.-Y.,Zhangzhou Health Vocational College | Huang Y.-Q.,Fujian Medical University | You Y.-H.,Fujian Medical University | Ma X.-D.,Fujian Medical University
Chinese Pharmacological Bulletin | Year: 2016

Aim: To investigate the effect of small interfering RNA(siRNA) targeting DNMT1 gene on cell proliferation, apoptosis and histone modulation in acute lymphoid leukemia cell line, Molt4. Methods: The small interfering RNA targeting DNMT1 gene was transfected into Molt-4 cells by Lipofectamine™ 2000. The DNMT1 mRNA and protein level were detected by RT-PCR and Western blot. Cell proliferation was determined by MTT. Cell apoptosis was measured by Flow Cytometry. The expression of Bcl-2, procaspase-3, P15 , histone methylation and histone acetylation was detected by Western blot. Results: DNMT1 was suppressed by siRNA targeting DNMT1 in a concentration-dependent manner. DNMT1 siRNA suppressed cells proliferation and induced apoptosis in Molt-4 cells. Apoptotic rate was (4.27 ± 1. 42)% , (15. 25 ±l.54)%, (35.63 ±2.54) %, (66. 27 ±3. 02)% after transfecting with DNMT1 siRNA at 0, 30, 60, 120 nmol·L-1 for 24 hours, P <0. 05. The expression of Bcl-2, procaspase-3 was suppressed and P15 was promoted after transfecting of DNMT1 siRNA. DNMT1 siRNA downregulated histone methylated H3K9 and upregulated histone methylated H3K4. The alteration of histone acetylation of H3 was not seen. Conclusion: DNMT1 siRNA suppresses DNMT1 efficiently in Molt4 cells. The depletion of DNMT1 downregulates histone methylation of H3K9, and upregulates histone methylation of H3K4. It inhibits cell growth and induces cell apoptosis in Molt4 cell line.

Zhou Z.,Xiamen University | Zhang H.,Zhangzhou Health Vocational College | Wang G.,Zhangzhou Entry exit Inspection and Quarantine Bureau | Zheng T.,Xiamen University
Mitochondrial DNA | Year: 2015

The complete mitochondrial genome of the Chinese lake gudgeon Sarcocheilichthys sinensis was first determined in this study. It is a circular DNA double strand of 16,684bp in length, encodes genes for 13 proteins, 2 ribosomal RNA subunits, 22 transfer RNAs and an A+T-rich control region with the typical gene order in vertebrate mitogenomes. Overall nucleotide composition is 30.5% A, 26.6% C, 16.7% G and 26.3% T. Three start codons (ATG, GTG and ATA) and three stop codons (TAG, TAA and T) were found in all protein-coding genes. The tRNA-SerAGY lacked the dihydrouridine arm and could not fold into typical cloverleaf secondary structure. The origin of L-strand replication was identified between the tRNA-Asn and tRNA-Cys genes. © 2013 Informa UK Ltd.

Lin M.Z.,Zhangzhou Health Vocational College | Zhu Y.,Xiamen University | Yang S.J.,Xiamen University | Wei D.M.,Taizhou University | Tian H.Q.,Xiamen University
Flora: Morphology, Distribution, Functional Ecology of Plants | Year: 2014

We analyzed anther development in Amomum villosum Lour. (Zingiberaceae) using the periodic acid-Schiff's technique and Sudan black staining to test for the presence of starch and lipids, respectively. Our analyses showed that microspore mother cells of A. villosum lack typical callose walls, and numerous lipid granules appear in the cells early in development. Some starch granules are present in anther wall cells, but not in tapetal cells. After meiosis, numerous lipid granules remain unchanged in the microspores. During microspore development, some small starch granules first appear in the central cell region, and then the starch granules increase in size. After microspore division, the bicellular pollen grains become filled with starch and lipids, and remain in this state until the pollen grains reach maturity. At anthesis, the anther wall of A. villosum consists of several layers of endothecium cells with an evidently thickened radial wall, and some layers of parenchyma cells containing numerous starch granules. © 2014 Elsevier GmbH.

Lin M.,Zhangzhou Health Vocational College | Wu Q.,Zhangzhou Health Vocational College | Zheng S.,Xiamen University | Tian H.,Xiamen University
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To establish an effective protocol for plant generation and induce polyploidy of Morinda officinalis. Method: Callus was induced from immature embryo of M. officinalis and polyploidy was inducted by using colchicine treatment method. Chromosome was detected by flow cytometry. Result and Conclusion: The highest induction rate of polyploidy was 18.40%, which was obtained with 500 mg·L -1 colchicine treatment for 5 days. Roots of polyploid were bigger than diploid. Advantages of using immature embryo as expiants are easy for sterilization, higher rate of callus induction and low degree dedifferentiation. The induced polyploidy of M. officinalis may have a value for spread of cultivation.

Lin M.-Z.,Zhangzhou Health Vocational College | Zheng S.,Xiamen University | Deng W.,Xiamen University | Tian H.-Q.,Xiamen University
Plant Physiology Communications | Year: 2010

Callus was induced from immature embryo of Morinda officinalis and then buds were induced from the callus. When the buds grew up, the stems were transplanted in a rooting medium to induce adventitious roots. The generation and the growth of adventitious roots were observed with paraffin method. The in vitro adventitious roots came from the cambial cells in culture.

Gao B.,Huaqiao University | Luo X.,Zhangzhou Health Vocational College | Fu H.,Huaqiao University | Chen Y.,Huaqiao University | And 2 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2014

Anatase TiO2 microspheres with a high percentage of exposed (001) facets were synthesized by a template-free hydrothermal method without using concentrated hydrofluoric acid. The influence of various experiment conditions, such as hydrothermal temperature, acid condition, reaction time, etc., on the morphology of the final products was investigated in detail. The formation mechanism of the microspheres was deduced according to the XRD, SEM, TEM, and HRTEM characterizations. The as-obtained microspheres exhibited superior photocatalytic activity in the degradation of Acid Red 88 compared to TiO2 without exposed (001) facet. Copyright © 2014 American Scientific Publishers.

Gao B.,Huaqiao University | Luo X.,Zhangzhou Health Vocational College | Fu H.,Huaqiao University | Lin B.,Huaqiao University | And 2 more authors.
Materials Research Bulletin | Year: 2013

Nitrogen-doped Ti1-xZrxO2 solid solutions have been synthesized by a multi-step sol-gel process followed by hydrothermal treatment in ammonia solution. XRD, XPS and UV-vis diffuse reflectance analyses indicated that nitrogen was doped in the surface layer of catalyst, introducing surface states located close to valence band. However, Zr4+ was successfully incorporated into the bulk lattice of TiO2 so as to induce the up-shift of conduction band. Compared to pristine TiO2 and nitrogen-doped TiO2, nitrogen-doped Ti1-xZr xO2 exhibited much higher efficiency for the degradation of Acid Red 88 solution and gaseous benzene under visible light irradiation, attributed to the synergetic effect of nitrogen and zirconium on the energy band. Specifically, the presence of surface states in the band gap enabled the extended visible light response and the up-shift of conduction band facilitated the excited electron interfacial transfer and hence suppressed efficiently the recombination of charge carriers. © 2012 Elsevier Ltd.

Fu H.,Huaqiao University | Luo X.-Z.,Zhangzhou Health Vocational College | Yuan X.,Huaqiao University | Shi J.-X.,Huaqiao University | Gao B.-F.,Huaqiao University
Gongneng Cailiao/Journal of Functional Materials | Year: 2013

Ce-doped TiO2 nanoparticles were prepared by sol-gel method. The structure and physicochemical properties of the catalysts were investigated by means of XRD, FT-IR, XPS, UV-Vis DRS, etc. The photocatalytic activities of Ce-doped TiO2 were evaluated by the degradation of acid red under visible light irradiation. The experiment results show that Ce-O-Ti bonds are formed on the interface of cerium oxide and TiO2, so that the crystallization and phase transition processes of the catalyst are impeded. Ce-TiO2 has a significant absorption in the visible region and the recombination of the photo-generated electron and hole pairs was greatly inhibited. Hence, the visible-light-driven photocatalytic efficiency of Ce-TiO2 was significantly enhanced. The catalyst doped with 20% Ce presents the best activity when calcinated at 400°C.

Xie J.,Fujian Medical University | Que W.,Fujian Medical University | Liu H.,Zhangzhou Health Vocational College | Liu M.,Second Hospital of Yulin | And 2 more authors.
Molecular Medicine Reports | Year: 2012

We extracted cucurmosin (CUS) from the sarcocarp of Cucrubita moschata (pumpkin). Recently, a number of studies have indicated that CUS has cytotoxic properties and induces apoptosis in a number of human tumor cells. However, the detailed mechanisms are largely unknown. The aim of this study was to confirm CUS's anticancer activity on human hepatoma HepG2 cells in vitro and in vivo, and to elucidate the mechanism of its activity. MTT was used to detect the cytotoxic effects of CUS. Flow cytometry was used to analyze cell apoptosis and the cell cycle. Transmission electron microscopy was used to observe the morphology of apoptotic cells. Western blot analysis was performed to measure the protein expression of bax, bcl-2 and procaspase-3. The established orthotopic transplantation models of human hepatoma in NOD/SCID mice were tested for anticancer activities in vivo. The results showed that CUS inhibited the proliferation of HepG2 cells in vitro and in vivo. CUS induced apoptosis and arrested the cell cycle. In addition, CUS increased the protein expression of bax, but decreased the bcl-2 and procaspase-3 expression in HepG2 cells. Our data indicate that CUS has potential anticancer activity for human hepatoma, which can be attributed in part to its inhibition of proliferation and apoptosis, induced by decreasing the bcl-2:bax ratio and caspase-3 activation.

PubMed | Fujian Medical University and Zhangzhou Health Vocational College
Type: Journal Article | Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2014

To detect the percentage of total natural killer (NK) cells and its different populations in the peripheral blood from neonates with bacterial pneumonia and discuss the clinical significance of NK cells in the pathogenesis of bacterial pneumonia.Flow cytometry was performed to detect the percentages of NK cells and its subsets in peripheral blood lymphocytes from 38 cases of neonatal bacterial pneumonias and 18 cases of normal neonates. Patients recruited were divided into two groups according to hospitalization days and numbers of peripheral leukocytes: hospitalization days within 10 days (including 10 days) as group A, and more than 10 days as group B; the number of peripheral blood leukocytes <5.010(9)/L or >20.010(9)/L as severe infection group, and 5.010(9)/L< number of peripheral blood leukocytes <20.010(9)/L as mild infection group.The percentages of peripheral blood NK cells and CD3(-)CD56(neg)CD16(bright) subset in the neonates with bacterial pneumonia were significantly lower than those of the normal newborns (P<0.01), but there were no statistically significant differences in CD3(-)CD56(bright)CD16(neg/dim) and CD3(-)CD56(dim)CD16(bright) subsets. The percentage of CD3(-)CD56(neg)CD16(bright) subset in group A was significantly lower than that of the normal newborns (P<0.01), while the percentages of the total NK cells and other subsets had no statistical significance. The neonates with bacterial pneumonia had significantly lower percentages of the total NK cells and CD3(-)CD56(neg)CD16(bright) subset in group B as compared with the normal neonates (P<0.01). And the percentages of the total NK cells and its subsets in group B were also lower than those in group A (P<0.05). The percentages of NK cells and each subset in severe infection group were significantly lower than those in mild infection group (P<0.05).To the neonates who suffer from bacterial pneumonia, the more serious and the longer hospital stay, the lower the percentages of NK cells and its subsets are.

Loading Zhangzhou Health Vocational College collaborators
Loading Zhangzhou Health Vocational College collaborators