Zhangjiagang Hospital of Traditional Chinese Medicine

Zhangjiagang, China

Zhangjiagang Hospital of Traditional Chinese Medicine

Zhangjiagang, China
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Dang B.,Soochow University of China | Dang B.,Zhangjiagang Hospital of Traditional Chinese Medicine | Chen W.,Zhangjiagang Hospital of Traditional Chinese Medicine | He W.,Zhangjiagang Hospital of Traditional Chinese Medicine | Chen G.,Soochow University of China
Neural Plasticity | Year: 2017

Traumatic brain injury (TBI) is a major cause of chronic disability. Worldwide, it is the leading cause of disability in the under 40s. Behavioral problems, mood, cognition, particularly memory, attention, and executive function are commonly impaired by TBI. Spending to assist, TBI survivors with disabilities are estimated to be costly per year. Such impaired functional outcomes following TBI can be improved via various rehabilitative approaches. The objective of the present paper is to review the current rehabilitation treatment of traumatic brain injury in adults. © 2017 Baoqi Dang et al.


Wang Z.,Soochow University of China | Meng C.-J.,Soochow University of China | Shen X.-M.,Peoples Hospital of Jiangsu Province | Shu Z.,Peoples Hospital of Jiangsu Province | And 8 more authors.
Journal of Molecular Neuroscience | Year: 2012

The current research aimed to investigate the role of hypoxia-inducible factor-1α (HIF-1α), aquaporin-4 (AQP-4), and matrix metalloproteinase-9 (MMP-9) in blood-brain barrier (BBB) dysfunction and cerebral edema formation in a rat subarachnoid hemorrhage (SAH) model. The SAH model was induced by injection of 0.3 ml fresh arterial, non-heparinized blood into the prechiasmatic cistern in 20 s. Anti-AQP-4 antibody, minocycline (an inhibitor of MMP-9), or 2-methoxyestradiol (an inhibitor of HIF-1α), was administered intravenously at 2 and 24 h after SAH. Brain samples were extracted at 48 h after SAH and examined for protein expressions, BBB impairment, and brain edema. Following SAH, remarkable edema and BBB extravasations were observed. Compared with the control group, the SAH animals have significantly upregulated expressions of HIF-1α, AQP-4, and MMP-9, in addition to decreased amounts of laminin and tight junction proteins. Brain edema was repressed after inhibition of AQP-4, MMP-9, or HIF-1α. Although BBB permeability was also ameliorated after inhibition of either HIF-1α or MMP-9, it was not modulated after inhibition of AQP-4. Inhibition of MMP-9 reversed the loss of laminin. Finally, inhibition of HIF-1α significantly suppressed the level of AQP-4 and MMP-9, which could induce the expression of laminin and tight junction proteins. Our results suggest that HIF-1α plays a role in brain edema formation and BBB disruption via a molecular signaling pathway involving AQP-4 and MMP-9. Pharmacological intervention of this pathway in patients with SAH may provide a novel therapeutic strategy for early brain injury. © 2012 Springer Science+Business Media, LLC.


Zeng Y.,Nanjing University | Zeng Y.,Jiangsu Cancer Hospital | Shen Z.-J.,Nanjing University | Gu W.-Z.,Zhangjiagang Hospital of Traditional Chinese Medicine | Wu M.-H.,Nanjing University
Translational Cancer Research | Year: 2017

Background: The mechanisms by which puerarin prevents and treats lung cancer remain largely unknown. We aimed to study the roles of puerarin in the invasion and metastasis of lung cancer A549 cells based on cyclooxygenase-2 (COX-2). Methods: The effects of different concentrations of puerarin on the proliferation of A549 cells were evaluated. The in vitro and in vivo effects of puerarin on the invasion and metastasis of A549 cells were assessed by Transwell assay and an animal model of tumor lung metastasis respectively. The influence of puerarin on COX-2 in A549 cells was also analyzed in terms of enzymatic activity and tissue level to explore the role of COX-2 in the inhibitory effects of puerarin on cell invasion and metastasis. Results: Puerarin inhibited the proliferation of A549 cells in time- and dose-dependent manners. Compared with the control group, more cells in the puerarin treatment groups were arrested in the G0/G1 phase, but the proportion of S phase cells decreased. Puerarin blocked the cell cycle dose-dependently. After 24 h of culture with 200 μg/mL puerarin, significantly fewer cells penetrated the basement membrane of Transwell chambers than those in the control group, and the MMP-2/TIMP-2 levels in PUE200, PUE400 and PUE800 groups significantly dropped. For the animal experiments, the puerarin treatment groups had significantly fewer and smaller tumors with pulmonary metastasis than those of the control group. After 24 h of culture with puerarin, like the indomethacin (IN) group, PUE200, PUE400 and PUE800 groups had significantly lower enzymatic activity of COX-2 than that of the control group, but the puerarin treatment groups had similar results. The IN group had lower COX-2 protein expression than that of the control group, but the puerarin treatment groups had similar expressions to that of the control group, indicating that COX-2 protein expression was hardly affected by puerarin in A549 cells. Similar to the IN group, the puerarin treatment groups had significantly lower PGE2 levels than that of the control group (P < 0.05). However, there was no significant difference between PUE200, PUE400 and PUE800 groups. The tumors with pulmonary metastasis from the puerarin treatment groups had significantly lighter colors of COX-2 positive cells and particles than those of the control group. Conclusions: Puerarin inhibited the enzymatic activity of COX-2 in A549 cells and its expression in tumor tissues with pulmonary metastasis. MMP-2/TIMP-2 may be one of the mechanisms by which puerarin inhibits the invasion of A549 cells. © Translational Cancer Research.


Zhao Q.-Y.,Zhangjiagang Hospital of Traditional Chinese Medicine | Sun H.,Jiangsu Province Hospital | Zhang Y.,Zhangjiagang Hospital of Traditional Chinese Medicine
International Eye Science | Year: 2017

AIM: To study the curative effect for patients with age related cataract and shallow anterior chamber after phacoemulsification. METHODS: Totally 38 patients (38 eyes) with age related cataract and shallow anterior chamber were selected and divided into two groups according to the depth of the anterior chamber, as mild shallow anterior chamber group (2-2.5mm) 23 eyes, high risk shallow anterior chamber group (<2.0mm) 15 eyes. Thirty-eight patients (38 eyes) with age related cataract with normal anterior chamber were as control group at the same period. All the patients received the operations by the same doctor and were followed up for 3mo. The observed items included visual acuity before and after operations, intraocular pressure, anterior chamber depth, corneal endothelial cell density and complications. RESULTS: There were no significant difference on visual acuity, intraocular pressure and corneal endothelial cell density between the two groups before operations (P>0.05). The visual acuity improved significantly after operation in both groups (P<0.05). Intraocular pressure after operation decreased significantly in both groups (P<0.05). Anterior chamber depth increased significantly after operation in both groups (P<0.05). Corneal endothelial cell density decreased significantly in both groups (P<0.05). There were no significant difference on anterior chamber depth, intraocular pressure and corneal endothelial cell density between the two groups at different time point after operations (P>0.05). Posterior capsular rupture occurred in shallow anterior chamber group in 1 eye, suspensory ligament rupture in 1 eye. Posterior capsular rupture and suspensory ligament rupture occurred none in normal anterior chamber group. Postoperative corneal edema occurred in 10 eyes (26%) in shallow anterior chamber group, which occurred in 3 eyes (8%) in normal anterior chamber group. The difference on the incidence was significant (P<0.05). CONCLUSION: Phacoemulsification should be taken timely for patients with age related cataract and shallow anterior chamber. The postoperative visual acuity can be improved and the anterior chamber depth can increase. The operation is safe and effective for those patients. Copyright 2017 by the IJO Press.


Wang Z.,Soochow University of China | Wang Z.,Zhangjiagang Hospital of Traditional Chinese Medicine | Wang G.,Soochow University of China | Yang H.,Soochow University of China
Journal of Clinical Neuroscience | Year: 2012

Thirty-one patients with osteoporotic vertebral compression fractures (OVCF) were treated with unilateral balloon kyphoplasty (BKP), and 31 patients were treated with bilateral BKP. The efficacy of unilateral and bilateral BKP was assessed by comparing operation time, X-ray exposure times, incidence of complications, vertebral height restoration, and improvement of the visual analogue scale (VAS) scores. The mean operative time and the exposure time to X-rays in the unilateral BKP group was less than that of the bilateral BKP group (p < 0.05). No statistically significant differences were observed in the cement leakage rate, VAS score, or vertebral height restoration between the two groups (p > 0.05). Unilateral and bilateral BKP are safe and effective treatments for OVCF. Compared with bilateral BKP, patients undergoing unilateral BKP have shorter operations and receive lower X-ray radiation doses. © 2011 Elsevier Ltd. All rights reserved.


Qiang S.,Zhangjiagang Hospital of Traditional Chinese Medicine | Du Z.-F.,Zhangjiagang Hospital of Traditional Chinese Medicine | Huang M.,Zhangjiagang Hospital of Traditional Chinese Medicine
Asian Pacific Journal of Tropical Medicine | Year: 2014

Objective: To investigate the inhibitory effects of adenovirus-mediated NDRG2 on the proliferation of human renal cell carcinoma cell line OS-RC-2 in vitro. Method: NDRG2 was harvested by RT-PCR, confirmed by DNA sequencing, and then cloned into the eukaryotic expression vector pIRES2-EGFP, which encodes green fluorescent protein (GFP), to construct pIRES2-EGFP-NDRG2 plasmid. OS-RC-2 cells with NDRG2 negative expression were transfected with pIRES2-EGFP-NDRG2 plasmid. The growth of transfected OS-RC-2 cells was observed under light and fluorescence microscopes. After colony-forming cell assays, cell proliferation detection and MTT assays, the growth curves of cells in each group were plotted to investigate the inhibitory effects of adenovirus-mediated NDRG2 on the proliferation of OS-RC-2 cells. Cell cycle was determined by flow cytometry. Confocal laser scanning microscopy showed that NDRG2 protein was specifically located on subcellular organelle. Results: A eukaryotic expression vector pIRES2-EGFP-NDRG2 was successfully constructed. After NDRG2 transfection, the growth of OS-RC-2 cells was inhibited. Flow cytometry showed that cells were arrested in S phase but the peak of cell apoptosis was not present, and confocal laser scanning microscopy showed that NDRG2 protein was located in mitochondrion. Conclusions: NDRG2 can significantly inhibit the proliferation of OS-RC-2 cells in vitro and its protein is specifically expressed in the mitochondrion. © 2014 Hainan Medical College.


Peng S.,Tongji University | Sun H.,Zhangjiagang Hospital of Traditional Chinese Medicine | Zhang X.,Tongji University | Liu G.,Xuzhou Medical College | Wang G.,Xuzhou Medical College
Cell Biochemistry and Biophysics | Year: 2014

Phosphodiesterase-4 (PDE-4) regulates the intracellular level of cyclic adenosine monophosphate. Recent studies demonstrated that PDE-4 inhibitors can counteract deficits in long-term memory caused by aging or increased expression of mutant forms of human amyloid precursor proteins, and can influence the process of memory function and cognitive enhancement. Therapeutics, such as ketamine, a drug used in clinical anesthesia, can also cause memory deficits as adverse effects. Targeting PDE-4 with selective inhibitors may offer a novel therapeutic strategy to prevent, slow the progress, and, eventually, treat memory deficits. © 2014 Springer Science+Business Media New York.


Wang Z.,Soochow University of China | Ma C.,Soochow University of China | Meng C.-J.,Soochow University of China | Zhu G.-Q.,Suzhou Municipal Hospital | And 8 more authors.
Journal of Pineal Research | Year: 2012

Melatonin has beneficial effects against early brain injury (EBI) by modulating cerebral oxidative stress after experimental subarachnoid hemorrhage (SAH); however, few investigations relate to the precise underlying molecular mechanisms. To date, the relation between melatonin and nuclear factor erythroid 2-related factor 2 and antioxidant responsive element (Nrf2-ARE) pathway has not been studied in SAH models. This study was undertaken to evaluate the influence of melatonin on Nrf2-ARE pathway in rats after SAH. Adult male SD rats were divided into four groups: (i) control group (n = 18); (ii) SAH group (n = 18); (iii) SAH + vehicle group (n = 18); and (iv) SAH + melatonin group (n = 18). The rat SAH model was induced by injection of 0.3 mL fresh arterial, nonheparinized blood into the prechiasmatic cistern in 20 s. In SAH + melatonin group, melatonin was administered i.p. at 150 mg/kg at 2 and 24 hr after the induction of SAH. Brain samples were extracted at 48 hr after SAH. Treatment with melatonin markedly increased the expressions of Nrf2-ARE pathway-related agents, such as Nrf2, heme oxygenase-1, NAD(P)H:quinone oxidoreductase 1, and glutathione S-transferase α-1. Administration of melatonin following SAH significantly ameliorated EBI, including brain edema, blood-brain barrier (BBB) impairment, cortical apoptosis, and neurological deficits. In conclusion, post-SAH melatonin administration may attenuate EBI in this SAH model, possibly through activating Nrf2-ARE pathway and modulating cerebral oxidative stress by inducing antioxidant and detoxifying enzymes. © 2012 John Wiley & Sons A/S.


Zhang Q.,Nanjing University of Technology | Chen X.,Nanjing University of Technology | Tang Y.,Zhangjiagang Hospital of Traditional Chinese Medicine | Ge L.,Nanjing University of Technology | And 2 more authors.
Analytica Chimica Acta | Year: 2014

A sandwich-type electrochemical immunosensor for the detection of carbohydrate antigen 19-9 (CA 19-9) antigen based on the immobilization of primary antibody (Ab1) on three dimensional ordered macroporous magnetic (3DOMM) electrode, and the direct electrochemistry of horseradish peroxidase (HRP) that was used as both the label of secondary antibody (Ab2) and the blocking reagent. The 3DOMM electrode was fabricated by introducing core-shell Au-SiO2@Fe3O4 nanospheres onto the surface of three dimensional ordered macroporous (3DOM) Au electrode via the application of an external magnet. Au nanoparticles functionalized SBA-15 (Au@SBA-15) was conjugated to the HRP labeled secondary antibody (HRP-Ab2) through the Au-SH or Au-NH3+ interaction, and HRP was also used as the block reagent. The formation of antigen-antibody complex made the combination of Au@SBA-15 and 3DOMM exhibit remarkable synergistic effects for accelerating direct electron transfer (DET) between HRP and the electrode. Under the optimal conditions, the DET current signal increased proportionally to CA 19-9 concentration in the range of 0.05 to 15.65UmL-1 with a detection limit of 0.01UmL-1. Moreover, the immunosensor showed high selectivity, good stability, satisfactory reproducibility and regeneration. Importantly, the developed method was used to assay clinical serum specimens, achieving a good relation with those obtained from the commercialized electrochemiluminescent method. © 2014 Elsevier B.V.


PubMed | Zhangjiagang Hospital of Traditional Chinese Medicine and Nanjing University of Traditional Chinese Medicine
Type: Journal Article | Journal: Journal of separation science | Year: 2016

A rapid and high sensitive ultra high performance liquid chromatography with tandem mass spectrometry method for the simultaneous determination of notoginsenoside R1 and ginsenoside Re in rat plasma was developed. The analytes and internal standard, digoxin, were extracted from rat plasma via protein precipitation with methanol and separated on an Phenomenex Gemini C18 column within 2 min. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and positive ion mode. The precursor to product ion transitions monitored for notoginsenoside R1, ginsenoside Re, and internal standard were m/z 955.5775.5, 969.6789.1, and 803.6283.1, respectively. The assay was validated with linear range of 1.9-380 ng/mL for notoginsenoside R1 and 0.5-100 ng/mL for ginsenoside Re. The intra- and interday precisions (RSD%) were within 8.96% for each analyte. The absolute recoveries were greater than 93% for R1 and 96% for Re. Each analyte was stable during all sample storage, preparation, and analytic procedures. The method was successfully applied to a pharmacokinetic study of Xuesaitong dispersible tablets in eight rats.

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