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Li Y.,Sun Yat Sen University | Han Y.-Y.,Zengcheng Peoples Hospital | Zhong J.-L.,Sun Yat Sen University | Shi J.,Sun Yat Sen University | Liu J.,Sun Yat Sen University
Chinese Journal of Medical Imaging Technology

Objective: To compare the value of pubococcygeal line (PCL) system and the H line, M line, organ prolapse (HMO) classification system of dynamic MR imaging for evaluation on pelvic floor structures in patients with pelvic floor dysfunction (PFD). Methods: Sixteen patients with PFD (PFD group) and 24 normal women (normal female group) underwent static and dynamic pelvic MRI. The pelvic organs and pelvic floor structure were observed conventionally. In PCL system, the distance of bladder neck, uterocervical and anorectal junction to PCL in the mid-sagittal plane of dynamic MRI were measured. In HMO system, the length of H line (the puborectal line, which represents the anteroposterior hiatal dimension line) and M line (the shortest distance between the posterior aspect of the puborectalis muscle sling and the PCL, which is a measure of pelvic floor descent) were measured, and the distances of organ to H line were measured. The results of two measuring methods were compared, and both were compared with clinical POP-Q (Pelvic Organ Prolapse Quantification) system. Results: In normal female group, bladder neck, uterocervical and anorectal junction mildly decreases during pelvic straining, the down value was (2.63±1.71)mm, (4.31±3.24)mm and (7.32±2.11)mm, respectively. H line and M line slightly increased about (2.85±2.62)mm and (7.33±2.14)mm. In PFD group, the descent of bladder neck, uterocervical and anorectal junction was (24.74±10.12)mm, (21.43±14.91)mm and (24.55±13.43)mm, respectively. M line and H line increased by (13.16±10.82)mm and (22.54±11.30)mm, respectively. PCL system diagnosed 9 cases of uterine prolapse, 7 cases of bladder prolapse and 6 cases of rectal prolapse, while HMO system diagnosed 4, 7 and 5 cases, respectively. There was no statistically significant difference in evaluation of bladder and rectal prolapse between the two methods (P=0.25, 0.06), but HMO system was better in evaluation of uterine prolapse (P=0.007). Conclusion: In normal female, the results of PCL system and HMO system are consistent. In patients with PFD, HMO system could be more accurately for thoroughly evaluation of pelvic floor disorders, which having the advantages of revealing all pelvic organs and pelvic floor musculature. Copyright © 2013 by the Press of Chinese Journal of Medical Imaging Technology. Source

He G.,Zengcheng Peoples Hospital | Guo W.,Peking University | Lou Z.,Peking University | Zhang H.,Peking University
Cell Biochemistry and Biophysics

Achyranthes bidentata, is a herbal plant commonly used in the treatment of osteoporosis and bone nonunion in the Traditional Chinese Medicine. Saponins are the major compounds extracted from Achyranthes bidentata that have been shown to exert various pharmacological activities such as anti-inflammatory, antipyretic, antirheumatic, diuretic, and anti-osteoporosis. The Achyranthes bidentata saponins (ABS) were found to induce proliferation and differentiation in bone marrow stromal cells (BMSCs) as determined by the cell proliferation and alkaline phosphatase assays. Also, following the osteogenic induction, cells treated with ABS showed increased mRNA levels of rat bone morphogenetic protein-2, runt-related transcription factor 2, and osterix. Furthermore, ABS stimulated the activation of ERK as evidenced by increased phosphorylation of these proteins, which was blocked by an inhibitor of ERK (PD98059). Taken together, these results suggest that ABS stimulated osteogenic differentiation of BMSCs via activation of the ERK signaling pathway. © 2014 Springer Science+Business Media New York. Source

He G.,Zengcheng Peoples Hospital | Chen X.,Putian University | Zhang G.,Putian University | Lin H.,Putian University | And 2 more authors.
Cell Biochemistry and Biophysics

The objective is to determine the relation between severity of knee osteoarthritis (KOA) and levels of Collagen type II metabolite (C2C) and trace elements in the urine. The urine sample and knee joint films (anteroposterior and lateral) from the KOA patients and control subjects were collected. The KOA patients were divided into five groups (controls and grades I-IV) according to the Kellgren-Lawrence radiographic grading standards. Urine levels of C2C and trace elements were detected by enzyme-linked immunosorbent assay and inductively coupled plasma atomic emission spectrometry, respectively. Urine C2C levels in the KOA subjects (261.235 ± 39.944 pg/ml) were higher than those of the control group (218.341 ± 22.270 pg/ml). The Fe content in KOA groups was significantly lower than that of control group (group IV > group III > group II > group I or controls). The contents of Cu and Zn were also significantly higher in the KOA patients than in the control group (p < 0.05). However, Cr, Al, Cd, Ni, and Se levels of KOA patients were not significantly different from those of the controls (p > 0.05). Determination of the urine levels of C2C and trace elements may prove to be informative for an early diagnosis of KOA. It can also assist in the prognosis judgment of the disease and selecting an appropriate therapeutic regimen. © 2014 Springer Science+Business Media New York. Source

Li H.-P.,Sun Yat Sen University | Zeng X.-C.,Zengcheng Peoples Hospital | Long J.-T.,Sun Yat Sen University | Zhou B.,Sun Yat Sen University | And 4 more authors.

It has been demonstrated that nuclear factor-kappa B (NF-κB), which is overactivated in hepatocellular carcinoma (HCC), plays important roles in the development of HCC. Recently, a group of dysregulated micro RNAs were reported to be involved in HCC progression. Further understanding of micro RNA-mediated regulation of NF-κB pathway may provide novel therapeutic targets for HCC. In this study, we found that miR-451 expression was markedly downregulated in HCC cells and tissues compared with immortalized normal liver epithelial cells and adjacent non-cancerous tissues, respectively. Upregulation of miR-451 inhibited, while downregulation of miR-451 promoted, the tumorigenicity of HCC cells both in vitro and in vivo. These changes in the properties of HCC cells were associated with deregulation of two well-known cellular G1/S transitional regulators, cyclin D1 and c-Myc, which are downstream targets of NF-κB pathway. Furthermore, we demonstrated that miR-451 upregulation led to downregulation of cyclin D1 and c-Myc through inhibition of NF-κB pathway initiated by direct targeting of the IKBKB 3′-untranslated region. Therefore, these results suggest that miR-451 downregulation plays an important role in promoting proliferation of HCC cells and may provide the basis for the development of novel anti-HCC therapies. © The Author 2013. Published by Oxford University Press. All rights reserved. Source

Ye P.,Sun Yat Sen University | Li J.-H.,Guangzhou University | Xu J.-H.,Zengcheng Peoples Hospital | Huang S.-H.,Sun Yat Sen University | And 4 more authors.
Chinese Journal of Tissue Engineering Research

BACKGROUND: Triggering receptor expressed on myeloid cells 2 (TREM-2) is highly expressed throughout the synovial tissue in active rheumatoid arthritis patients, but the role of TREM-2 in the pathogenesis of rheumatoid arthritis still remains unclear. OBJECTIVE: To explore the TREM-2 expression in the synovial tissue of collagen type II-induced arthritis rats. METHODS: The collagen-induced arthritis models were established in rats. The activity indicators and pathological changes of arthritis synovial were dynamically observed. The mRNA levels of TREM-2, tumor necrosis factor-α, interleukin-1β, and interleukin-10 were detected in synovial tissue of rats by RT-PCR. The protein expression and location of TREM-2 were measured with western blot assay and immunohistochemistry, respectively. RESULTS AND CONCLUSION: At day 13 after immunization, the paws of model rats appeared red and swelling, the arthritis index scores were increased (P < 0.01). At day 19-25 after immunization, the inflammation reached the peak. Hematoxylin-eosin staining showed that, the synovium of collagen-induced arthritis rats were proliferated and were infiltrated by inflammatory cells, cartilage was destroyed. Compared with the control group, the expression of TREM-2 mRNA and protein, the mRNA levels of tumor necrosis factor-α and interleukin-1β in synovial tissue of the model rats were significantly increased (P < 0.05 or P < 0.01), while interleukin-10 mRNA expression was significantly decreased (P < 0.05). Experimental findings indicate that, TREM-2 is a crucial inflammatory regulator and the increasing expression of TREM-2 plays an important role in the pathogenesis of collagen-induced arthritis. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

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