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Zitka O.,Zemedelska | Zitka O.,Mendel University in Brno | Merlos M.-A.,CSIC - Experimental Station of El Zaidín | Adam V.,Zemedelska | And 13 more authors.
Journal of Hazardous Materials | Year: 2012

Aim of the present paper was to study the electrochemical behavior of copper(II) induced complexes in extracts obtained from mycorrhizal and non-mycorrhizal maize (Zea mays L.) plants grown at two concentrations of copper(II): physiological (31.7. ng/mL) and toxic (317 μg/mL). Protein content was determined in the plant extracts and, after dilution to proper concentration, various concentrations of copper(II) ions (0, 100, 200 and 400 μg/mL) were added and incubated for 1 h at 37 °C. Further, the extracts were analyzed using flow injection analysis with electrochemical detection. The hydrodynamic voltammogram (HDV), which was obtained for each sample, indicated the complex creation. Steepness of measured dependencies was as follows: control 317 μg/mL of copper < control 31.7 ng/mL of copper < mycorrhizal 31.7 ng/mL of copper < mycorrhizal 317 μg/mL of copper. Based on these results it can be concluded that mycorrhizal fungus actively blocks transport copper(II) ions to upper parts of a plant by means of adsorbing of copper(II) in roots. Rapid complex formation was determined under applied potentials 300, 500 and 600. mV during the measuring HDVs. It was also verified that mycorrhizal colonization reduced root to shoot translocation of Cu(II) ions. © 2011 Elsevier B.V.


Konecna L.,Zemedelska | Kuchtik J.,Zemedelska | Kralickova S.,Zemedelska | Pokorna M.,Zemedelska | And 3 more authors.
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2013

The aim of the study was to evaluate the effect of different crossbreeds (CB) of Lacaune (LC) and East Friesian (EF) breeds (LC 50 EF 50, LC 75 EF 25 and LC 87.5 EF 12.5) on daily milk yield (DMY) and basic milk composition (contents of total solids (TS), fat (F), protein (P), casein (C) and lactose (L) for the whole lactation. The evaluation of the interactions between CB and parity (PA) and between CB and litter size (LS) on above mentioned indicators was also an integral part of this study. The study was carried out on the organic farm in Valašská Bystrice. The factor of CB had a signifi cant effect only on DMY and contents of P and C for the whole lactation whilst the higher proportion of LC breed in genotype, the higher DMY and the lower P and C contents. The CB x PA had a signifi cant effect on all indicators except for the lactose content when in all CB the DMY increased with the increase of the lactation number. On the other hand, in all CB the contents of TS, F, P and C were relatively very variable depending on the parity. Lastly, as for the interaction between CB and LS, the ewes with twins produced signifi cantly more milk compared to ewes with singles and these ewes had also signifi cantly higher contents of P and C and not signifi cantly higher contents of TS and F.


Kucera A.,Zemedelska | HolikC L.,Zemedelska | Marosz K.,Zemedelska | Martinik A.,Mendel University in Brno | Vavricek D.,Zemedelska
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2013

The article deals with the reaction of soil environment to a violent deforestation resulting from a wind storm. As a material, permanent inventory plot located in Training Forest Enterprise Masaryk Forest Krtiny was selected. The plot represents beech high forest, where soil was sampled from four types of sample plots: (1) maternal forest representing situation before the storm; (2) zone of transition from the maternal forest to the open area; (3) reafforested clearing; (4) natural evolution. From each sample plot type, 6 mixed samples of Ah horizon were analysed to assess N-ammonium (N-NH4 +) and N-nitrates (NO3 -) content and respiration activity. The results show a signifi cant difference between the respiration activities of the particular sample plots, as well as a signifi cant difference in the content of N-ammonium and N-nitrate forms, the maternal forest representing a site of the lowest biological (and respiration) activity on the one hand, and, on the other hand, site of high N-ammonium and low N-nitrate content, respectively. From the results, intensive nitrifi cation caused by the deforestation is evident. The results are to be used as a starting level for a long-term observation of reaction of the forest beech ecosystem to deforestation and selected types of forest management.


PubMed | Zemedelska
Type: | Journal: Journal of hazardous materials | Year: 2012

Aim of the present paper was to study the electrochemical behavior of copper(II) induced complexes in extracts obtained from mycorrhizal and non-mycorrhizal maize (Zea mays L.) plants grown at two concentrations of copper(II): physiological (31.7 ng/mL) and toxic (317 g/mL). Protein content was determined in the plant extracts and, after dilution to proper concentration, various concentrations of copper(II) ions (0, 100, 200 and 400 g/mL) were added and incubated for 1h at 37C. Further, the extracts were analyzed using flow injection analysis with electrochemical detection. The hydrodynamic voltammogram (HDV), which was obtained for each sample, indicated the complex creation. Steepness of measured dependencies was as follows: control 317 g/mL of copper

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