Zebra Bioscience BV

Enschede, Netherlands

Zebra Bioscience BV

Enschede, Netherlands
SEARCH FILTERS
Time filter
Source Type

Dongre C.,University of Twente | Van Weerd J.,Zebra Bioscience BV | Besselink G.A.J.,LioniX BV | Vazquez R.M.,CNR Institute for Photonics and Nanotechnologies | And 6 more authors.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2011

We introduce a principle of parallel optical processing to an optofluidic lab-on-a-chip. During electrophoretic separation, the ultra-low limit of detection achieved with our set-up allows us to record fluorescence from covalently end-labeled DNA molecules. Different sets of exclusively color-labeled DNA fragments - otherwise rendered indistinguishable by spatio-temporal coincidence - are traced back to their origin by modulation-frequency-encoded multi-wavelength laser excitation, fluorescence detection with a single ultrasensitive, albeit color-blind photomultiplier, and Fourier analysis decoding. As a proof of principle, fragments obtained by multiplex ligation-dependent probe amplification from independent human genomic segments, associated with genetic predispositions to breast cancer and anemia, are simultaneously analyzed. © 2011 The Royal Society of Chemistry.


Dongre C.,MESA Institute for Nanotechnology | Van Weerd J.,Zebra Bioscience BV | Besselink G.A.J.,LioniX BV | Van Weeghel R.,Zebra Bioscience BV | And 7 more authors.
Electrophoresis | Year: 2010

By applying integrated-waveguide laser excitation to an optofluidic chip, fluorescently labeled DNA molecules of 12 or 17 different sizes are separated by CE with high operating speed and low sample consumption of ∼600 pL. When detecting the fluorescence signals of migrating DNA molecules with a PMT, the LOD is as low as 2.1 pM. In the diagnostically relevant size range (∼150-1000 base-pairs) the molecules are separated with reproducibly high sizing accuracy (>99%) and the plug broadening follows Poissonian statistics. Variation of the power dependence of migration time on base-pair size - probably with temperature and condition of the sieving gel matrix - indicates that the capillary migration cannot be described by a simple physical law. Integrated-waveguide excitation of a 12-μm narrow microfluidic segment provides a spatio-temporal resolution that would, in principle, allow for a 20-fold better accuracy than the currently supported by state-of-the-art electrophoretic separation in microchips, thereby demonstrating the potential of this integrated optical approach to fulfill the resolution demands of future electrophoretic microchips. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.


Dongre C.,MESA Institute for Nanotechnology | Van Weerd J.,Zebra Bioscience BV | Besselink G.A.J.,LioniX BV | Martinez Vazquez R.,CNR Institute for Photonics and Nanotechnologies | And 6 more authors.
Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010 | Year: 2010

Implementing capillary electrophoresis with high base-pair resolution and integrated multi-wavelength excitation in an optofluidic chip, we separate simultaneously sets of end-labeled DNA fragments from independent human genomic segments, relevant for diagnosing breast cancer and anemia. © 2010 Optical Society of America.


Dongre C.,MESA Institute for Nanotechnology | van Weerd J.,Zebra Bioscience BV | Bellini N.,CNR Institute for Photonics and Nanotechnologies | Osellame R.,CNR Institute for Photonics and Nanotechnologies | And 4 more authors.
Biomedical Optics Express | Year: 2010

We present a simple approach in electrophoretic DNA separation and fluorescent monitoring that allows to identify the insertion or deletion of base-pairs in DNA probe molecules from genetic samples, and to perform intrinsic calibration/referencing for highly accurate DNA analysis. The principle is based on dual-point, dual-wavelength laser-induced fluorescence excitation using one or two excitation windows at the intersection of integrated waveguides and microfluidic channels in an optofluidic chip and a single, color-blind photodetector, resulting in a limit of detection of ~200 pM for single-end-labeled DNA molecules. The approach using a single excitation window is demonstrated experimentally, while the option exploiting two excitation windows is proposed theoretically. © 2010 Optical Society of America.

Loading Zebra Bioscience BV collaborators
Loading Zebra Bioscience BV collaborators