Time filter

Source Type

Li C.,Xuzhou Medical College | Zhang H.-B.,Peoples Hospital of Jiangsu Province | Zhang H.,Zaozhuang Mining Group Central Hospital | Li Q.,Xuzhou Medical College | And 4 more authors.
Orthopaedics and Traumatology: Surgery and Research | Year: 2016

Aim: To evaluate the treatment strategy for pathological fractures caused by vertebral hemangiomas (VHs) using large case series. Methods: From January 2008 to January 2014, 28 patients suffering from severe pathological fractures (more than 2/3 loss of original vertebral height) due to thoracic or lumbar VHs were randomized to an experimental (the posterior decompression, bone cement augmentation and internal fixation, n = 14) or control (only the posterior decompression combined with internal fixation, n = 14) group. The anterior, middle vertebral body height, kyphosis angle and the cement leakage were measured on radiography. Visual analogue scale (VAS), 36-item short form (SF-36) and Oswestry disability index (ODI) were recorded to assess the pain relief, life quality and function improvement. Results: Compared with the preoperation, the anterior, middle vertebral body height and kyphosis angle were significantly improved after two procedures, but the improvement efficacy seemed to be more significant in the experimental group, with no significant loss of correction effect at final follow-up. The VAS, SF-36 and ODI scores were all significantly improved postoperatively, especially at final follow-up in two groups. The neurological situation was improved in patients at least 1 grade in Frankel scale. After mean follow-up of 24 months, no operative complications (internal fixation loosening, breakage, spinal nerve damage or pneumothorax) were observed, except bone cement leakage occurred in two cases in experimental group. Conclusion: Posterior decompression, bone cement augmentation and internal fixation seems to be effective and safe for pathological fractures caused by VHs, with better outcomes and few complications. © 2016 Elsevier Masson SAS.


PubMed | Peoples Hospital of Jiangsu Province, Zaozhuang Mining Group Central Hospital, Xuzhou Medical College and Shanghai JiaoTong University
Type: Journal Article | Journal: Orthopaedics & traumatology, surgery & research : OTSR | Year: 2016

To evaluate the treatment strategy for pathological fractures caused by vertebral hemangiomas (VHs) using large case series.From January 2008 to January 2014, 28patients suffering from severe pathological fractures (more than 2/3 loss of original vertebral height) due to thoracic or lumbar VHs were randomized to an experimental (the posterior decompression, bone cement augmentation and internal fixation, n=14) or control (only the posterior decompression combined with internal fixation, n=14) group. The anterior, middle vertebral body height, kyphosis angle and the cement leakage were measured on radiography. Visual analogue scale (VAS), 36-item short form (SF-36) and Oswestry disability index (ODI) were recorded to assess the pain relief, life quality and function improvement.Compared with the preoperation, the anterior, middle vertebral body height and kyphosis angle were significantly improved after two procedures, but the improvement efficacy seemed to be more significant in the experimental group, with no significant loss of correction effect at final follow-up. The VAS, SF-36 and ODI scores were all significantly improved postoperatively, especially at final follow-up in two groups. The neurological situation was improved in patients at least 1 grade in Frankel scale. After mean follow-up of 24months, no operative complications (internal fixation loosening, breakage, spinal nerve damage or pneumothorax) were observed, except bone cement leakage occurred in two cases in experimental group.Posterior decompression, bone cement augmentation and internal fixation seems to be effective and safe for pathological fractures caused by VHs, with better outcomes and few complications.


Chen P.,Zaozhuang Mining Group Central Hospital | Xi Q.,Fudan University | Wang Q.,Zaozhuang Mining Group Central Hospital | Wei P.,Zaozhuang Mining Group Central Hospital
Medical Oncology | Year: 2014

Dysregulation of microRNA-100 (miR-100) has been shown to be involved in cancer tumorigenesis and progression of several cancer types. However, its expression patterns in tumors are controversial. The aim of this study was to investigate the expression and clinical significance of miR-100 in colorectal cancer (CRC). Quantitative real-time PCR was used to analyze the expression of miR-100 in 138 pairs of human CRC and adjacent normal tissues. The prognostic values of miR-100 in CRC were also analyzed. The results showed that the miR-100 expression was significantly downregulated in CRC tissues when compared to adjacent normal tissues (P < 0.001). Also, low miR-100 expression was observed to be significantly correlated with larger tumor size (P = 0.023), higher incidence of lymph node metastasis (P = 0.009), and advanced TNM stage (P = 0.016). More importantly, Kaplan–Meier analysis showed that CRC patients with low miR-100 expression tended to have shorter overall survival. In multivariate analysis stratified for known prognostic variables, low miR-100 expression was identified as an independent prognostic factor for overall survival. In conclusion, our data indicated for the first time that the downregulation of miR-100 was associated with advanced clinical features and poor prognosis of CRC patients, suggesting that miR-100 downregulation may serve as an unfavorable prognostic biomarker in CRC. © 2014, Springer Science+Business Media New York.


Yang S.,Shandong University | Yang S.,Zaozhuang Mining Group Central Hospital | Li J.,Shandong University
World Chinese Journal of Digestology | Year: 2016

AIM: To assess the diagnostic value of microvascular blood flow signals in focal hepatic lesions. METHODS: Eighty patients diagnosed with 166 focal liver lesions by magnetic resonance imaging (MRI) and laboratory examination at our hospital from January 2015 to January 2016 were included. All patients received ordinary ultrasound and ultrasound microvascular imaging, and the diagnostic significance of these diagnostic methods was analyzed. RESULTS: Ultrasound microvascular imaging had a higher detection rate for hepatic focal lesions than conventional ultrasound (97.59% vs 66.27%). In patients with malignant lesions after the use of contrast agents, mean time to enhancement, total duration of enhancement, and time to visualization of enhancement were significantly shorter than those of benign disease patients (P < 0.05). CONCLUSION: Ultrasound microvascular imaging of hepatic focal lesions has a high sensitivity and specificity, and can be used for accurate diagnosis of the type, number and size of hepatic focal lesions. © 2016 Baishideng Publishing Group Inc. All rights reserved.


Fu Z.,Zaozhuang Mining Group Central Hospital | Qian F.,Zaozhuang Mining Group Central Hospital | Yang X.,Zaozhuang Mining Group Central Hospital | Jiang H.,Zaozhuang Mining Group Central Hospital | And 2 more authors.
Medical oncology (Northwood, London, England) | Year: 2014

Previous studies have revealed the significance of circulating microRNAs as biomarkers for cancers. The aim of this study was to detect the levels of circulating microRNA-222 (miR-222) in plasma of gastric cancer (GC) patients and evaluate its diagnostic and prognostic value. Levels of circulating miR-222 were detected by using qRT-PCR in plasma of 114 GC patients, 36 chronic atrophic gastritis (CAG) patients and 56 healthy controls. The result showed that the expression of circulating miR-222 in plasma was significantly upregulated in GC compared with CAG and healthy controls (all at P < 0.001). And its high level was significantly correlated with clinical stages (P < 0.001) and lymph nodes metastasis (P = 0.009). The receiver operating characteristics (ROC) curve analyses revealed that miR-222 had considerable diagnostic accuracy, yielded an AUC (the areas under the ROC curve) of 0.850 with 66.1 % sensitivity and 88.3 % specificity in discriminating GC from healthy controls. Moreover, Kaplan-Meier analysis demonstrated a correlation between increased circulating miR-222 level and reduced disease-free survival (P = 0.016) and overall survival (P = 0.012). In multivariate analysis stratified for known prognostic variables, circulating miR-222 was identified as an independent prognostic marker. In conclusion, our findings suggested that circulating miR-222 in plasma might be a potential and useful noninvasive biomarker for the early detection and prognosis of GC.


Chen P.,Zaozhuang Mining Group Central Hospital | Xi Q.,Zaozhuang Mining Group Central Hospital | Wang Q.,Zaozhuang Mining Group Central Hospital | Wei P.,Zaozhuang Mining Group Central Hospital
Medical oncology (Northwood, London, England) | Year: 2014

Dysregulation of microRNA-100 (miR-100) has been shown to be involved in cancer tumorigenesis and progression of several cancer types. However, its expression patterns in tumors are controversial. The aim of this study was to investigate the expression and clinical significance of miR-100 in colorectal cancer (CRC). Quantitative real-time PCR was used to analyze the expression of miR-100 in 138 pairs of human CRC and adjacent normal tissues. The prognostic values of miR-100 in CRC were also analyzed. The results showed that the miR-100 expression was significantly downregulated in CRC tissues when compared to adjacent normal tissues (P<0.001). Also, low miR-100 expression was observed to be significantly correlated with larger tumor size (P=0.023), higher incidence of lymph node metastasis (P=0.009), and advanced TNM stage (P=0.016). More importantly, Kaplan-Meier analysis showed that CRC patients with low miR-100 expression tended to have shorter overall survival. In multivariate analysis stratified for known prognostic variables, low miR-100 expression was identified as an independent prognostic factor for overall survival. In conclusion, our data indicated for the first time that the downregulation of miR-100 was associated with advanced clinical features and poor prognosis of CRC patients, suggesting that miR-100 downregulation may serve as an unfavorable prognostic biomarker in CRC.


Chu F.,Shandong University | Wang X.,Shandong University | Sun Q.,Shandong University | Liang H.,Shandong University | And 7 more authors.
Clinical and Experimental Hypertension | Year: 2016

A fibrinolytic enzyme was purified from the dry body of Whitmania pigra Whitman. The fibrinolytic enzyme was purified to homogeneity with a yield of 0.003% and a purification of 630.7 fold. The molecular weight of the enzyme was estimated to be 26.7 kDa by reduced sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was tested by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and it showed that the enzyme was a novel fibrinolytic enzyme. The optimal pH and temperature of the enzyme were 8.5 and 55°C, respectively. Enzyme activity was enhanced by Na+, Mg2+, and K+. On the contrary, the proteolytic activity was significantly inhibited by Mn2+, Fe2+, Fe3+, ethylenediaminetetraacetic acid (EDTA), and ethylenebis(oxyethylenenitrilo)tetraacetic acid (EGTA). Fibrinolytic and fibrinogenolytic assays showed that the enzyme preferentially hydrolyzed fibrinogen Aα-chains, followed by Bβ- and γ-chains. The α-, β-, and γ-γ-chains of fibrin were also degraded by the enzyme. © 2016 Taylor & Francis.


PubMed | Zaozhuang Mining Group Central Hospital
Type: Journal Article | Journal: Journal of B.U.ON. : official journal of the Balkan Union of Oncology | Year: 2015

The tumor suppressor gene p16 is frequently silenced and inactivated by hypermethylation in human cancers, including prostate cancer. However, the association between the methylation status of p16 and prostate cancer risk remains ambiguous. This study aimed to assess the association of p16 methylation with prostate cancer risk by a comprehensive metaanalysis.Relevant studies were identified by searching PubMed, Embase and Web of Science databases before October 2014 with no restrictions. The strength of the association between p16 methylation and prostate cancer risk was assessed by combined odds ratio (OR) and 95 % confidence interval (CI). The between-study heterogeneity and the contributions of single studies to the final results were tested by chi-square-based Q test and sensitivity analyses, respectively. Publication bias was evaluated by funnel plots.A total of 1,296 samples from 12 independent studies were enrolled in the present metaanalysis. Overall, a significant association was observed between p16 methylation and prostate cancer risk (OR=3.06; 95% CI:1.34- 6.98;p=0.008). Stratified analyses by ethnic groups further revealed that prostate cancer risk was increased for individuals carrying the methylated p16 compared with those with unmethylated p16 in Caucasian populations (OR=2.51;95% CI:1.01-6.26;p=0.047) and Asian populations (OR=9.50;95% CI:1.78-50.61;p=0.008).This study identified a strong association of p16 methylation with prostate cancer risk and suggested that p16 methylation might be a potential biomarker for prostate cancer.


PubMed | Zaozhuang Mining Group Central Hospital
Type: Journal Article | Journal: Medical oncology (Northwood, London, England) | Year: 2014

Previous studies have revealed the significance of circulating microRNAs as biomarkers for cancers. The aim of this study was to detect the levels of circulating microRNA-222 (miR-222) in plasma of gastric cancer (GC) patients and evaluate its diagnostic and prognostic value. Levels of circulating miR-222 were detected by using qRT-PCR in plasma of 114 GC patients, 36 chronic atrophic gastritis (CAG) patients and 56 healthy controls. The result showed that the expression of circulating miR-222 in plasma was significantly upregulated in GC compared with CAG and healthy controls (all at P < 0.001). And its high level was significantly correlated with clinical stages (P < 0.001) and lymph nodes metastasis (P = 0.009). The receiver operating characteristics (ROC) curve analyses revealed that miR-222 had considerable diagnostic accuracy, yielded an AUC (the areas under the ROC curve) of 0.850 with 66.1 % sensitivity and 88.3 % specificity in discriminating GC from healthy controls. Moreover, Kaplan-Meier analysis demonstrated a correlation between increased circulating miR-222 level and reduced disease-free survival (P = 0.016) and overall survival (P = 0.012). In multivariate analysis stratified for known prognostic variables, circulating miR-222 was identified as an independent prognostic marker. In conclusion, our findings suggested that circulating miR-222 in plasma might be a potential and useful noninvasive biomarker for the early detection and prognosis of GC.

Loading Zaozhuang Mining Group Central Hospital collaborators
Loading Zaozhuang Mining Group Central Hospital collaborators