Zaozhuang Mining Group Central Hospital

Jinan, China

Zaozhuang Mining Group Central Hospital

Jinan, China
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Liu Z.-H.,Fuzhou General Hospital of Nanjing Command PLA | Li X.-J.,Fuzhou General Hospital of Nanjing Command PLA | Li X.-J.,Zaozhuang Mining Group Central Hospital | Huang A.-W.,Fuzhou General Hospital of Nanjing Command PLA | And 2 more authors.
Current Drug Delivery | Year: 2017

Purpose: This study aimed to develop a synchronized and sustained-release silymarin dropping pill, and to evaluate its pharmacokinetic characteristics. Method: Polyoxyethylene stearate, glyceryl monostearate, and stearic acid were used to prepare the dropping pills. X-ray powder diffraction, differential scanning calorimetry, and release were used to evaluate its physicochemical properties. The plasma concentration of silybin in beagle dogs after oral administration of silymarin dropping pills and silymarin capsule was determined by RP-HPLC. Results: Synchronized release was achieved with high similarity factor f2 values between every set of two of the five components. Mean plasma concentration-time curves of silymarin after oral administration of dropping pills in beagle dogs were in accordance with first-order absorption and open two-compartment model. The Tmax, Cmax, and AUC0-∞ of dropping pills in beagle dogs were 0.8750±0.13 h, 0.8183±0.07 µg·ml-1, and 2.274±0.90 µg·h·ml-1, respectively. Silymarin dropping pills prolonged in vivo exposure and reduced maximum in vivo concentration, achieving a stable level in the serum. Conclusion: The combination of solid dispersion technique and dropping pill formulation allowed synchronized release of multiple components in herbal medicine, and has potential application in the development of sustained release in herbal medicine. © 2017 Bentham Science Publishers.


Zhu R.-X.,Zaozhuang Mining Group Central Hospital | Zhao L.,Dalian Medical University | Zhang Y.-K.,Taian Central Hospital | Luo P.,Shanghai Topgen Biopharm Co. | And 2 more authors.
Bangladesh Journal of Pharmacology | Year: 2015

Combination therapy has prolonged the survival of patients with small cell lung cancer (SCLC), an aggressive neoplasm characterized by a high rate of metastasis. In the present study the effect of sequential treatment of ursolic acid chlorophenyl triazole (UACT) followed by 5-fluorouracil (5-FU) on human small cell lung cancer cells was investigated. The results revealed a synergistic effect of the sequential treatment with UACT and 5-FUcombination on cytotoxic activities, NF-kB protein activation, repression of TNF-induced NF-kB-dependent reporter gene expression, and TNF-induced COX-2, MMP-9 and Cyclin D1 activation in H209 cells. The synergism in apoptotic cell death was observed in H209, H69, 87-5,and Lu135 cells. The synergistic effect of UACT and 5-FU was observed at a concentration of 50 nM of UACT and 20 μM of 5-FU. These results indicate that UACT and 5-FU combination can be a promising chemotherapeutic regimen in the treatment of SCLC. © 2015, Bangladesh Pharmacological Society. All right reserved.


Feng W.,Zaozhuang Mining Group Central Hospital | Liu S.,Zaozhuang Mining Group Central Hospital | Zhu R.,Zaozhuang Mining Group Central Hospital | Li B.,Zaozhuang Mining Group Central Hospital | And 3 more authors.
Biochemical and Biophysical Research Communications | Year: 2017

The mechanisms modulating the cancer stem cell (CSC) properties of triple negative breast cancer (TNBC) cells were not fully understood. In this study, we performed data mining in Breast Cancer Gene-Expression Miner v4.0 and found that TNBC tumors had significantly higher NES mRNA expression than other breast cancer subtypes. Pooled data suggested that NES mRNA expression is associated worse metastatic relapse (MR) free survival and also worse any event (AE) free survival in TNBC patients. Following data mining in multiple big data databases confirmed a positive correlation between SOX10 mRNA expression and NES mRNA expression in breast cancer tissues. In addition, the expression of SOX10 mRNA is significantly higher in TNBC tissues than in other breast cancer subtypes. SOX10 overexpression resulted in Nestin upregulation at both mRNA and protein levels. Bioinformatic analysis predicted a SOX10 binding site in NES promoter and the following dual luciferase assay verified the binding site. Functionally, SOX10 overexpression substantially increased CSC properties of TNBC cells, while SOX10 knockdown decreased the CSC properties, in terms of CD24-/CD44+ cell ratio and tumorsphere-forming capabilities. Enforced Nestin expression partly counteracted the effect of SOX10 knockdown on reducing the CSC properties. Based on these findings, we infer that SOX10 regulates cancer stem cell properties of TNBC cells via inducing Nestin expression. © 2017 Elsevier Inc.


PubMed | Peoples Hospital of Jiangsu Province, Zaozhuang Mining Group Central Hospital, Xuzhou Medical College and Shanghai JiaoTong University
Type: Journal Article | Journal: Orthopaedics & traumatology, surgery & research : OTSR | Year: 2016

To evaluate the treatment strategy for pathological fractures caused by vertebral hemangiomas (VHs) using large case series.From January 2008 to January 2014, 28patients suffering from severe pathological fractures (more than 2/3 loss of original vertebral height) due to thoracic or lumbar VHs were randomized to an experimental (the posterior decompression, bone cement augmentation and internal fixation, n=14) or control (only the posterior decompression combined with internal fixation, n=14) group. The anterior, middle vertebral body height, kyphosis angle and the cement leakage were measured on radiography. Visual analogue scale (VAS), 36-item short form (SF-36) and Oswestry disability index (ODI) were recorded to assess the pain relief, life quality and function improvement.Compared with the preoperation, the anterior, middle vertebral body height and kyphosis angle were significantly improved after two procedures, but the improvement efficacy seemed to be more significant in the experimental group, with no significant loss of correction effect at final follow-up. The VAS, SF-36 and ODI scores were all significantly improved postoperatively, especially at final follow-up in two groups. The neurological situation was improved in patients at least 1 grade in Frankel scale. After mean follow-up of 24months, no operative complications (internal fixation loosening, breakage, spinal nerve damage or pneumothorax) were observed, except bone cement leakage occurred in two cases in experimental group.Posterior decompression, bone cement augmentation and internal fixation seems to be effective and safe for pathological fractures caused by VHs, with better outcomes and few complications.


Chen P.,Zaozhuang Mining Group Central Hospital | Xi Q.,Fudan University | Wang Q.,Zaozhuang Mining Group Central Hospital | Wei P.,Zaozhuang Mining Group Central Hospital
Medical Oncology | Year: 2014

Dysregulation of microRNA-100 (miR-100) has been shown to be involved in cancer tumorigenesis and progression of several cancer types. However, its expression patterns in tumors are controversial. The aim of this study was to investigate the expression and clinical significance of miR-100 in colorectal cancer (CRC). Quantitative real-time PCR was used to analyze the expression of miR-100 in 138 pairs of human CRC and adjacent normal tissues. The prognostic values of miR-100 in CRC were also analyzed. The results showed that the miR-100 expression was significantly downregulated in CRC tissues when compared to adjacent normal tissues (P < 0.001). Also, low miR-100 expression was observed to be significantly correlated with larger tumor size (P = 0.023), higher incidence of lymph node metastasis (P = 0.009), and advanced TNM stage (P = 0.016). More importantly, Kaplan–Meier analysis showed that CRC patients with low miR-100 expression tended to have shorter overall survival. In multivariate analysis stratified for known prognostic variables, low miR-100 expression was identified as an independent prognostic factor for overall survival. In conclusion, our data indicated for the first time that the downregulation of miR-100 was associated with advanced clinical features and poor prognosis of CRC patients, suggesting that miR-100 downregulation may serve as an unfavorable prognostic biomarker in CRC. © 2014, Springer Science+Business Media New York.


Yang S.,Shandong University | Yang S.,Zaozhuang Mining Group Central Hospital | Li J.,Shandong University
World Chinese Journal of Digestology | Year: 2016

AIM: To assess the diagnostic value of microvascular blood flow signals in focal hepatic lesions. METHODS: Eighty patients diagnosed with 166 focal liver lesions by magnetic resonance imaging (MRI) and laboratory examination at our hospital from January 2015 to January 2016 were included. All patients received ordinary ultrasound and ultrasound microvascular imaging, and the diagnostic significance of these diagnostic methods was analyzed. RESULTS: Ultrasound microvascular imaging had a higher detection rate for hepatic focal lesions than conventional ultrasound (97.59% vs 66.27%). In patients with malignant lesions after the use of contrast agents, mean time to enhancement, total duration of enhancement, and time to visualization of enhancement were significantly shorter than those of benign disease patients (P < 0.05). CONCLUSION: Ultrasound microvascular imaging of hepatic focal lesions has a high sensitivity and specificity, and can be used for accurate diagnosis of the type, number and size of hepatic focal lesions. © 2016 Baishideng Publishing Group Inc. All rights reserved.


Fu Z.,Zaozhuang Mining Group Central Hospital | Qian F.,Zaozhuang Mining Group Central Hospital | Yang X.,Zaozhuang Mining Group Central Hospital | Jiang H.,Zaozhuang Mining Group Central Hospital | And 2 more authors.
Medical oncology (Northwood, London, England) | Year: 2014

Previous studies have revealed the significance of circulating microRNAs as biomarkers for cancers. The aim of this study was to detect the levels of circulating microRNA-222 (miR-222) in plasma of gastric cancer (GC) patients and evaluate its diagnostic and prognostic value. Levels of circulating miR-222 were detected by using qRT-PCR in plasma of 114 GC patients, 36 chronic atrophic gastritis (CAG) patients and 56 healthy controls. The result showed that the expression of circulating miR-222 in plasma was significantly upregulated in GC compared with CAG and healthy controls (all at P < 0.001). And its high level was significantly correlated with clinical stages (P < 0.001) and lymph nodes metastasis (P = 0.009). The receiver operating characteristics (ROC) curve analyses revealed that miR-222 had considerable diagnostic accuracy, yielded an AUC (the areas under the ROC curve) of 0.850 with 66.1 % sensitivity and 88.3 % specificity in discriminating GC from healthy controls. Moreover, Kaplan-Meier analysis demonstrated a correlation between increased circulating miR-222 level and reduced disease-free survival (P = 0.016) and overall survival (P = 0.012). In multivariate analysis stratified for known prognostic variables, circulating miR-222 was identified as an independent prognostic marker. In conclusion, our findings suggested that circulating miR-222 in plasma might be a potential and useful noninvasive biomarker for the early detection and prognosis of GC.


Chen P.,Zaozhuang Mining Group Central Hospital | Xi Q.,Zaozhuang Mining Group Central Hospital | Wang Q.,Zaozhuang Mining Group Central Hospital | Wei P.,Zaozhuang Mining Group Central Hospital
Medical oncology (Northwood, London, England) | Year: 2014

Dysregulation of microRNA-100 (miR-100) has been shown to be involved in cancer tumorigenesis and progression of several cancer types. However, its expression patterns in tumors are controversial. The aim of this study was to investigate the expression and clinical significance of miR-100 in colorectal cancer (CRC). Quantitative real-time PCR was used to analyze the expression of miR-100 in 138 pairs of human CRC and adjacent normal tissues. The prognostic values of miR-100 in CRC were also analyzed. The results showed that the miR-100 expression was significantly downregulated in CRC tissues when compared to adjacent normal tissues (P<0.001). Also, low miR-100 expression was observed to be significantly correlated with larger tumor size (P=0.023), higher incidence of lymph node metastasis (P=0.009), and advanced TNM stage (P=0.016). More importantly, Kaplan-Meier analysis showed that CRC patients with low miR-100 expression tended to have shorter overall survival. In multivariate analysis stratified for known prognostic variables, low miR-100 expression was identified as an independent prognostic factor for overall survival. In conclusion, our data indicated for the first time that the downregulation of miR-100 was associated with advanced clinical features and poor prognosis of CRC patients, suggesting that miR-100 downregulation may serve as an unfavorable prognostic biomarker in CRC.


PubMed | Zaozhuang Mining Group Central Hospital
Type: Journal Article | Journal: Journal of B.U.ON. : official journal of the Balkan Union of Oncology | Year: 2015

The tumor suppressor gene p16 is frequently silenced and inactivated by hypermethylation in human cancers, including prostate cancer. However, the association between the methylation status of p16 and prostate cancer risk remains ambiguous. This study aimed to assess the association of p16 methylation with prostate cancer risk by a comprehensive metaanalysis.Relevant studies were identified by searching PubMed, Embase and Web of Science databases before October 2014 with no restrictions. The strength of the association between p16 methylation and prostate cancer risk was assessed by combined odds ratio (OR) and 95 % confidence interval (CI). The between-study heterogeneity and the contributions of single studies to the final results were tested by chi-square-based Q test and sensitivity analyses, respectively. Publication bias was evaluated by funnel plots.A total of 1,296 samples from 12 independent studies were enrolled in the present metaanalysis. Overall, a significant association was observed between p16 methylation and prostate cancer risk (OR=3.06; 95% CI:1.34- 6.98;p=0.008). Stratified analyses by ethnic groups further revealed that prostate cancer risk was increased for individuals carrying the methylated p16 compared with those with unmethylated p16 in Caucasian populations (OR=2.51;95% CI:1.01-6.26;p=0.047) and Asian populations (OR=9.50;95% CI:1.78-50.61;p=0.008).This study identified a strong association of p16 methylation with prostate cancer risk and suggested that p16 methylation might be a potential biomarker for prostate cancer.


PubMed | Zaozhuang Mining Group Central Hospital
Type: Journal Article | Journal: Medical oncology (Northwood, London, England) | Year: 2014

Previous studies have revealed the significance of circulating microRNAs as biomarkers for cancers. The aim of this study was to detect the levels of circulating microRNA-222 (miR-222) in plasma of gastric cancer (GC) patients and evaluate its diagnostic and prognostic value. Levels of circulating miR-222 were detected by using qRT-PCR in plasma of 114 GC patients, 36 chronic atrophic gastritis (CAG) patients and 56 healthy controls. The result showed that the expression of circulating miR-222 in plasma was significantly upregulated in GC compared with CAG and healthy controls (all at P < 0.001). And its high level was significantly correlated with clinical stages (P < 0.001) and lymph nodes metastasis (P = 0.009). The receiver operating characteristics (ROC) curve analyses revealed that miR-222 had considerable diagnostic accuracy, yielded an AUC (the areas under the ROC curve) of 0.850 with 66.1 % sensitivity and 88.3 % specificity in discriminating GC from healthy controls. Moreover, Kaplan-Meier analysis demonstrated a correlation between increased circulating miR-222 level and reduced disease-free survival (P = 0.016) and overall survival (P = 0.012). In multivariate analysis stratified for known prognostic variables, circulating miR-222 was identified as an independent prognostic marker. In conclusion, our findings suggested that circulating miR-222 in plasma might be a potential and useful noninvasive biomarker for the early detection and prognosis of GC.

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