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Wojciak K.M.,University Przyrodniczy w Lublinie | Dolatowski Z.J.,University Przyrodniczy w Lublinie | Kolozyn-Krajewska D.,Zaklad Higieny i Zarzadzania Jakoscia Zywnosci
Zywnosc. Nauka. Technologia. Jakosc/Food. Science Technology. Quality | Year: 2014

The objective of the research study was to evaluate the oxidative stability of dry-fermented sausage with acid whey or probiotic strain (Lb. casei ŁOCK 0900) added during a four-month period of chilling storage (4° C). Four experimental varieties of the product were manufactured: K pickled sausage as a control sample (2.8 % of pickling salts); L sausage with sea salt (2.8 %) and Lb. casei ŁOCK 0900 (log 6.3 cfu/g) probiotic strain; S sausage with the sea salt (2.8 %) and acid whey (5.0 %); LG sausage with the sea salt (2.8 %), probiotic strain, and glucose (0.6 %). The research covered the determination of the pH value, water activity (aw), oxidation-reduction potential (ORP), peroxide value (PV), TBARS value, reflectancy spectrum as well as the oxidation value and 650/570 nm index to characterize the colour parameters of the product after ripening process (0) and after the four-month period of chilling storage. The count of lactic acid bacteria (LAB) was also determined after the four-month storage period. Compared to other samples, a significantly lower (p < 0.001) pH value was reported in the sample with the probiotic bacteria and glucose (LC) added, and in the sample with the acid whey (S) added immediately after dry-fermenting and during storage period. Compared to other samples, significantly lower peroxide values were reported in the control sample (K) and in the sample with probiotic (L) after dryfermenting process. Except for the sample with probiotic and glucose (LG), in all the products the peroxide value increased significantly by ca. 2.0 meqO2/kg after the four-month storage period. Compared to other samples, significantly higher TBARS values were determined in the samples with the acid whey (S) added as well as in the samples with probiotic and glucose (LC) added. The oxidation processes were the slowest in the pickled control sample (K). In the sample with the strain of Lb. casei ŁOCK 0900 added, the variant with the glucose (LC) added was characterized by the lowest oxidation value and 650/570 nm index; this fact proved that the colour developed in the product was similar to the colour of the pickled product. The highest 650/570 nm index was obtained in the sample with the addition of acid whey (S) after dry-fermenting and after the four-month storage period. The oxidation stability of the samples evaluated decreased as follows: K > LG > L > S. In all the studied samples, the number of lactic acid bacteria was reported to be higher than log 7.0 cfu/g. © 2014, Polish Food Technologist Society. All rights reserved. Source


Neffe-Skocinska K.,Zaklad Higieny i Zarzadzania Jakoscia Zywnosci | Kolozyn-Krajewska D.,Zaklad Higieny i Zarzadzania Jakoscia Zywnosci | Goryl A.,Cracow University of Economics
Zywnosc. Nauka. Technologia. Jakosc/Food. Science Technology. Quality | Year: 2013

The objective of the research study was to assess the effect of ripening conditions on the survival of Lb. casei LOCK 0900 probiotic strain in pork loins and on their sensory quality during 180 days of chilling storage under anaerobic conditions. Also, it was attempted to construct mathematical models of growth and survival of the bacteria studied. The experimental material comprised Lb. casei ŁOCK 0900 probiotic strain and pork loins ripened at varying temperatures (16, 20, and 24 °C) and, next, vacuum packaged and stored at 4 °C for 180 days. Control samples with the addition of glucose and experimental samples with the addition of a probiotic strain and glucose were prepared. The scope of research included microbiological analyses, pH measurement, and sensory evaluation (QDA). The determinations were performed every 30 days. It was found that the best storage stability, both in terms of microbiological and sensory quality, was obtained in the case of loins with probiotic bacteria added, which were produced at 20 °C temperature of ripening (7.00 - 8,00 log jtk/g on average; overall sensory quality > 7 c.u.). A satisfactory model of growth and survival of LAB was constructed for the loins, which ripened at 24 °C (matching at a level of C24 = 98 %, P24 = 96 %). Source


Neffe K.,Zaklad Higieny i Zarzadzania Jakoscia Zywnosci | Kolozyn-Krajewska D.,Zaklad Higieny i Zarzadzania Jakoscia Zywnosci
Zywnosc. Nauka. Technologia. Jakosc/Food. Science Technology. Quality | Year: 2010

The objective of the research conducted was to assess the potential growth and survival of selected probiotic strains in ripening meat products using an example of pork loins. The research material comprised raw pork loins and two probiotic strains Lactobacillus casei LOCK 0900 (Patent No.: P-382760) and Lactobacillus casei ŁOCK 0908 (Patent No.: P-382760). Three kinds of loin samples were analyzed in the experiment: two control samples (loins with and without 0.2% glucose additive), two samples with only Lactobacillus casei ŁOCK 0900 and ŁOCK 0908 probiotic strains added, and the samples with the probiotic strain and 0.2% glucose added. The loin samples prepared in this way ripened for 3 weeks at a temperature of 16°C. Then, the products analyzed were vacuum-packed and stored at 4° C during a period of 6 months. Three series of experiment were performed. Micro-biological analyses had to determine the count of lactic acid bacteria (LAB). They were made 3 weeks after the completion of the 3-week meat ripening process and 6 months after the storage of the vacuum-packed loins using TEMPO® (Biomerieux, France), i.e. an automated system of measuring the count of micro-organisms. It was found that the probiotic strains of Lactobacillus casei ŁOCK 0900 and ŁOCK 0908, added to the cured pork loins, grew and achieved a count of 107 log cfu/g, and in the samples with the additive of 0.2% glucose, their count was 108 log cfu/g. The count of probiotic bacteria in loins stored during a period of 6 months was by 1 to 2 log levels lower compared to the products after the ripening process, but this count was still high enough to consider those products to be probiotic. Source


SzydLowska A.,Zaklad Higieny i Zarzadzania Jakoscia Zywnosci | KoLozyn-Krajewska D.,Zaklad Higieny i Zarzadzania Jakoscia Zywnosci
Zywnosc. Nauka. Technologia. Jakosc/Food. Science Technology. Quality | Year: 2010

The objective of this study was to develop a pumpkin pulp fermented by probiotic bacteria as a semifinished product to be used to make sorbets. It was found that the optimal parameters for pumpkin pulp to ferment using one probiotic bacterial strain of Lactobacillus casei KN 291 are as follows: temperature: 32°C; fermentation time: 26 h; sucrose additive: 8 %. The above fermentation parameters were applied and enabled to produce a pumpkin pulp of the highest overall sensory acceptance. During the fermentation process, the increase in the bacteria count occurred depending on the type of pumpkin pulp; this count increased to the value of 9.55 - 9.90 cfu /g. A higher Lactobacillus casei KN 291 count was reported in the pumpkin pulps with inuline, and this fact may evidence the preobiotic additive to have an advantageous effect of on the growth of bacteria. Source

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