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Pandey V.,Govind Ballabh Pant University of Agriculture & Technology | Ansari M.W.,Zakir Husain Delhi College | Tula S.,Plant Molecular Biology Group | Sahoo R.K.,Plant Molecular Biology Group | And 4 more authors.
Plant Signaling and Behavior | Year: 2016

Ocimum leaves are highly enriched in antioxidant components. Thus, its leaf extract, if applied in plants, is believed to efficiently scavenge ROS, thereby preventing oxidative damage under drought stress. Thus, the present study was performed in kharif 2013 and rabi 2014 season to evaluate the effect of aqueous leaf extract of Ocimum sanctum against drought stress in 2 rice genotype under glass house conditions. Here we show that various morpho- physiological (chlorophyll fluorescence, leaf rolling score, leaf tip burn, number of senesced leaves and total dry matter) and biochemical parameters (proline, malondialdehyde and superoxide dismutase content) were amended by Ocimum treatment in both the seasons. Application of Ocimum extract increased expression of dehydrin genes, while reducing expression of aquaporin genes in drought stressed rice plant. Thus, application of Ocimum leaf extract under drought stress can be suggested as a promising strategy to mitigate drought stress in economical, accessible and ecofriendly manner. © 2016 Taylor & Francis Group, LLC. Source

Anwar M.S.,Govind Ballabh Pant University of Agriculture & Technology | Kapri A.,Govind Ballabh Pant University of Agriculture & Technology | Chaudhry V.,National Botanical Research Institute | Mishra A.,National Botanical Research Institute | And 6 more authors.
Protoplasma | Year: 2015

Thermoplastic-based materials are recalcitrant in nature, which extensive use affect environmental health. Here, we attempt to compare the response of indigenously produced bacterial consortium-I and consortium-II in degrading polyvinyl chloride (PVC). These consortia were developed by using different combination of bacterial strains of Pseudomonas otitidis, Bacillus cereus, and Acanthopleurobacter pedis from waste disposal sites of Northern India after their identification via 16S rDNA sequencing. The progressive degradation of PVC by consortia was examined via scanning electron microscopy, atomic force microscopy, UV–vis, FT-IR spectra, gel permeation chromatography, and differential scanning calorimetry analysis at different incubations and time intervals. The consortium-II was superior over consortium-I in degrading the PVC. Further, the carbon source utilization analysis revealed that the extensive use of consortia has not any effect on functional diversity of native soil microbes. © 2015 Springer-Verlag Wien Source

Bansal P.,Punjabi University | Gaurav G.,Punjabi University | Nidhi N.,Zakir Husain Delhi College | Malik A.K.,Punjabi University | Matysik F.-M.,University of Regensburg
Chromatographia | Year: 2012

A rapid and efficient analytical method suitable for the simultaneous determination of 1,3,5-trinitroperhydro- 1,3,5-triazine (RDX) and 2,4,6-trinitrotoluene (TNT) in groundwater and human plasma samples using liquid chromatography with UV detection (LC-UV) has been developed. RDX and TNT were baseline separated within time span of 4.8 min on a reverse phase C 18 analytical column with water acetonitrile mixture in ratio 30:70 (ν/ν) as mobile phase. The UV detection was carried out at 234 nm. A sample preparation method for biological and environmental matrices using microextraction by packed sorbent (MEPS) technique has been implemented, employing 4 mg of C 18 silica sorbent inserted into a microvolume syringe and using only 30 lL of plasma and groundwater samples. Several factors affecting the performance of MEPS, such as number of extraction cycles, washing solvent volume, elution solvent volume and carryover etc., were optimized. Limits of detection were 0.0997 and 0.0628 ng mL -1 for RDX and TNT, respectively. The extraction yields were satisfactory for both analytes (>81.2 %) as well as the precision data, which were always in the low percentage of relative standard deviation values (<5.6 %, n = 6). The present method is miniaturized, fully automated, and robust which can be easily used for forensic studies of explosives in environmental and biological samples. © Springer-Verlag 2012. Source

Chandra S.,Zakir Husain Delhi College | Tomar P.K.,Zakir Husain Delhi College | Kumar A.,Zakir Husain Delhi College | Malik A.,Zakir Husain Delhi College | Singh A.,Zakir Husain Delhi College
Monatshefte fur Chemie | Year: 2013

A complex of copper(II) with p-hydroxyacetophenone thiosemicarbazone has been used to fabricate a polymeric membrane electrode (PME) and coated glassy carbon electrode (CGCE). The proposed electrodes showed excellent response characteristics to thiocyanate ions over a wide concentration range from 1.0 × 10-7 to 1.0 × 10-1 M for PME and 1.0 × 10-6 to 1.0 × 10-2 M for CGCE. Nernstian slopes exhibited were -59.7 ± 0.3 mV decade-1 for PME and -53.6 ± 0.4 mV decade-1 for CGCE with low detection limits of 4.3 × 10-8 and 4.1 × 10-7 M, respectively. The PME membrane electrode provides a more sensitive and stable device than the CGCE electrode. The PME has a wide functional pH range (2.5-10.5), fast response time (5 s), and could be used for approximately 4 months without any considerable divergence in the potential. The selectivity coefficient was determined by a matched potential method and fixed interference method. A good discriminating ability towards the SCN- ion compared to other anions has been observed. PME was successfully applied as an indicator electrode for potentiometric titration of thiocyanate ion with AgNO3. Further, the electrode was successfully applied to determine the thiocyanate content in physiological fluids (urine samples). Graphical abstract: [Figure not available: see fulltext.] © 2013 Springer-Verlag Wien. Source

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