Zabol University of Medical Sciences
Zabol, Iran
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Setzer M.S.,University of Alabama in Huntsville | Sharifi-Rad J.,Zabol University of Medical Sciences | Setzer W.N.,University of Alabama in Huntsville
Antibiotics | Year: 2016

Recently, the emergence and spread of pathogenic bacterial resistance to many antibiotics (multidrug-resistant strains) have been increasing throughout the world. This phenomenon is of great concern and there is a need to find alternative chemotherapeutic agents to combat these antibiotic-resistant microorganisms. Higher plants may serve as a resource for new antimicrobials to replace or augment current therapeutic options. In this work, we have carried out a molecular docking study of a total of 561 antibacterial phytochemicals listed in the Dictionary of Natural Products, including 77 alkaloids (17 indole alkaloids, 27 isoquinoline alkaloids, 4 steroidal alkaloids, and 28 miscellaneous alkaloids), 99 terpenoids (5 monoterpenoids, 31 sesquiterpenoids, 52 diterpenoids, and 11 triterpenoids), 309 polyphenolics (87 flavonoids, 25 chalcones, 41 isoflavonoids, 5 neoflavonoids, 12 pterocarpans, 10 chromones, 7 condensed tannins, 11 coumarins, 30 stilbenoids, 2 lignans, 5 phenylpropanoids, 13 xanthones, 5 hydrolyzable tannins, and 56 miscellaneous phenolics), 30 quinones, and 46 miscellaneous phytochemicals, with six bacterial protein targets (peptide deformylase, DNA gyrase/topoisomerase IV, UDP-galactose mutase, protein tyrosine phosphatase, cytochrome P450 CYP121, and NAD+-dependent DNA ligase). In addition, 35 known inhibitors were docked with their respective targets for comparison purposes. Prenylated polyphenolics showed the best docking profiles, while terpenoids had the poorest. The most susceptible protein targets were peptide deformylases and NAD+-dependent DNA ligases. © 2016 by the authors; licensee MDPI, Basel, Switzerland.

Darbandi M.A.,Zabol University of Medical Sciences | Zandkarimi M.,Zabol University of Medical Sciences
Journal of Drug Delivery Science and Technology | Year: 2012

To increase the local therapeutic effect and reduce the systemic side effects of rifampicin (RIF), an anti-tuberculosis drug, lung targeting was investigated in the present study. Poly lactide-co-glycolide (PLGA) microparticles containing RIF were prepared by spray-drying method, mixed with an equal volume of lactose monohydrate (LAC) and delivered to the lung as a dry powder inhaler (DPI). Various ratios of ethyl acetate (EA), and dichloromethane (DCM) were used as solvent throughout the microparticle preparation. The use of large fractions of EA resulted in a slower evaporation rate and led to the formation of more spherical microparticles. Furthermore, the density of the microparticles and process yield increased, and release rate was reduced. In vivo experiments showed that changing the solvent of PLGA 75:25 from pure DCM to DCM/EA 67:33 mixture resulted in altering the T max value from 36 to 48 h, Cmax from 053 to 0.43 μg/mL, and local drug concentration at 60 h from 406 to 612 μg/gram tissue.

Imenshahidi M.,Mashhad University of Medical Sciences | Qaredashi R.,Mashhad University of Medical Sciences | Hashemzaei M.,Zabol University of Medical Sciences | Hosseinzadeh H.,Mashhad University of Medical Sciences
Jundishapur Journal of Natural Pharmaceutical Products | Year: 2014

Background: It has been elucidated that Berberis vulgaris (barberry) can alleviate morphine withdrawal syndrome. Also it has been reported that aqueous extract of barberry possibly have inhibitory effect on NMDA receptors. Objectives: In this study, we decided to evaluate the effects of aqueous extract of B. vulgaris fruit on morphine tendency in mice using conditioned place preference (CPP) method. Materials and Methods: In experiment 1 (acquisition phase), mice underwent morphine-induced conditioned place preference (CPP) training with injections of morphine (40 mg/kg). In experiment 2 (extinction and reinstatement phases), mice underwent the same CPP training as in experiment 1 and subsequent extinction training on day 16th a reinstatement by CPP was done by injection of reminding 10 mg/kg morphine. Results: The administration of morphine (40 mg/kg for four days) produced place preference. In the first method, the aqueous extract of barberry (200 mg/kg) prevented morphine tendency to white cell in CPP method. In the second method, after inter-peritoneal injection of aqueous extracts of barberry at 100 and 200 mg/kg, the animals tendency toward the white cells of CPP chamber on the sixteenth day (after a reminder injection of morphine 10 mg/kg) was significantly reduced. Conclusions: These results show that aqueous extract of barberry can reduce the acquisition and reinstatement of morphine-induced conditioned place preference. © 2014, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences.

Arbabisarjou A.,Zahedan University of Medical Sciences | Balouchi A.,Zabol University of Medical Sciences
Der Pharmacia Lettre | Year: 2015

The social networks are considered as one of the latest communication devices and all communities, especially students, have been widely involved in them. The social networks are called as a valuable source for learning but they include some risks as well out of them the foremost risk is the influence of these networks on students and their educational performance. This study was conducted in order to analyze the effect of social networks on educational performance of students. A case-control study was conducted on 190 high school students from Nikshahr and Zabol cities in Sistan and Balouchestan Province at southeast of Iran in winter 2015. The students were divided into two groups in which the first group acquired higher GPA (115 students) and the second group had lower GPA (75 students). Sampling was done by means of simple randomized method. The standardized questionnaire was the tool for data collection that analyzed the performance and application of social networks among students. the statistical test were used in this study including descriptive test of frequency, frequency percentage, independent T-test, and chi-square for analysis of the effect of social networks on educational performance of two groups. In case group, most of students were usingwhat's-up network in terms of type of the membership network; however, most of students in control group were members of Face-Book. Using of games was the paramount reason for application of social networks while education and training was the main reason for their use in control group. Whereas application of social networks in this study has led to reducing educational performance of students thus it is suggested to hold some classes to familiarize them with administration of using these networks and their application for training and learning of textbook topics.

Sahraie-Rad M.,Shahid Beheshti University of Medical Sciences | Izadyari A.,Islamic Azad University at Āmol | Rakizadeh S.,University of Tehran | Sharifi-Rad J.,Zabol University of Medical Sciences
Jundishapur Journal of Natural Pharmaceutical Products | Year: 2015

Background: Nowadays more than half of the populations in the world are suffering from dandruff. Malassezia fungus is an important factor in causing dandruff as it may stimulate cytokine production by keratinocytes (epidermal cells that synthesize keratin) and inflammatory pathways. As dandruff is a hurting issue for people, a lot of efforts are being done for treatment. Many reports based on medicinal plants, seem to be more effective in curing mentioned concern. Objectives: The aim of this research was to investigate a clinical trial for dandruff therapy by Zinc L-pyrrolidone carboxylic acid (Zinc-PCA) and pirocton olamine in combination with six medicinal plant extracts. Patients and Methods: In this study, the combination of Punica granatum L, Rosmarinus officinalis L, Matricaria chamomilla L, Urtica dioica L, Mentha piperita L., and Salvia officinalis L. methanolic extracts with Pirocton Olamine and Zinc-PCA in the shampoo form were tested on 30 patients with dandruff on hair within a period of two months. Chronic dandruff of 15 patients were highly removed in the second week, 12 other patients suffering from dandruff were seen with dandruff removal after 28 days, while remaining sufferers expressed satisfaction at the end of the fifth week. Results: Based on the results, medicinal plant extracts have marvelous effects on dandruff removal with fewer side effects, though it takes long time to treat the mentioned concern. Conclusions: The results show that applying the combination of medicinal plant extracts and natural ingredients with chemical compounds in pharmaceutical industry can optimize treatment.

Balouche A.,Zabol University of Medical Sciences
International Journal of Clinical Skills | Year: 2017

Background Renal osteodystrophy is a set of metabolic bone disorders occurs in patients with chronic kidney disease. Aim This study aimed to investigate the causes of renal osteodystrophy in hemodialysis patients in Zabol, in south east of Iran. Methods A total of 60 patients with End Stage Renal disease (ESRD), aged between 11-85 years entered this cross-sectional study. Patients were evaluated through a questionnaire, for age, sex, marital status, education level, occupation, income -as personal factors- cause of kidney disease, co-morbidities -as disease related factors- duration of dialysis, the number of dialysis per week, number of visits by specialists and medications -as dialysis based subjects. Blood samples were taken for determination of serum calcium, phosphorus, alkaline phosphatase, creatinine, albumin, total protein, parathyroid hormone and blood sugar. Vitamin D level and PTH level were used to determine bone disease. Data were analyzed using SPSS 21 software. Results This study showed that 48 patients (80%) were diagnosed with renal osteodystrophy. About half of the patients (47%) had osteofibrosis, 7% and 26% of subjects had a dynamic bone disease and osteomalacia, respectively. There was no statistically significant relationship between the presence of renal osteodystrophy and age (P = 0.7), gender (P = 0.3), marital status (P = 0.4), educational level (P = 0.4), occupation (P = 0.4), income (P = 0.2), duration of dialysis (P = 0.4), the number of dialysis per week (P = 0.45), number of visits (P = 0.7), underlying disease (P=0.35) and medications (P=0.3). Overall, PTH level was significantly higher in osteodystrophic patients comparing other ESRD patients (P < 0.001). Conclusions The study found that the high prevalence of renal osteodystrophy in our patients is not due to the interventions that happen after starting hemodialysis. © 2017.

Hashemzaei M.,Zabol University of Medical Sciences | Entezari Heravi R.,Zabol University of Medical Sciences | Rezaee R.,North Khorasan University of Medical Sciences | Roohbakhsh A.,Mashhad University of Medical Sciences | Karimi G.,Mashhad University of Medical Sciences
European Journal of Pharmacology | Year: 2017

Autophagy is a lysosomal degradation process through which long-lived and misfolded proteins and organelles are sequestered, degraded by lysosomes, and recycled. Autophagy is an essential part of cardiomyocyte homeostasis and increases the survival of cells following cellular stress and starvation. Recent studies made clear that dysregulation of autophagy in the cardiovascular system leads to heart hypertrophy and failure. In this manner, autophagy seems to be an attractive target in the new treatment of cardiovascular diseases. Although limited activation of autophagy is generally considered to be cardioprotective, excessive autophagy leads to cell death and cardiac atrophy. Natural products such as resveratrol, berberine, and curcumin that are present in our diet, can trigger autophagy via canonical (Beclin-1-dependent) and non-canonical (Beclin-1-independent) pathways. The autophagy-modifying capacity of these compounds should be taken into consideration for designing novel therapeutic agents. This review focuses on the role of autophagy in the cardioprotective effects of these compounds. © 2017 Elsevier B.V.

We analyzed the impact of interleukin (IL)-18 promoter polymorphisms on IL-18 serum levels in Helicobacter pylori-infected duodenal ulcer (DU) patients and healthy asymptomatic (AS) carriers. We also aimed to determine the association of the H. pylori virulence factors CagA and VacA antibodies with serum concentrations of IL-18 in order to elucidate any correlation between them. Three groups of patients were enrolled: DU patients (67 individuals), AS carriers (48 individuals), and H. pylori-negative subjects (26 individuals). Serum concentrations of IL-18 were determined by ELISA. Patient sera were tested by Western blot method to determine the presence of serum antibodies to bacterial CagA and VacA. Genotyping of IL-18 promoter polymorphisms at positions - 137G/C and - 607C/A were performed by allele-specific primer PCR protocol. Our study revealed that serum IL-18 levels are positively influenced by CagA-positive H. pylori strains, so that maximum levels of IL-18 were detected in DU patients with the CagA(+) phenotype, regardless of the presence of the anti-VacA antibody. Regarding IL-18 promoter polymorphisms, the AA genotype and A allele at position - 607C/A were found to be significantly lower in DU patients than in AS carriers and H. pylori-negative subjects (p = 0.032 and 0.043, respectively). The IL-18 - 607C variant was associated with higher levels of serum IL-18 and an increased risk of DU. Moreover, our findings indicated that serum concentrations of IL-18 were influenced by CagA factor, irrespective of the VacA status, suggesting that high levels of IL-18 in CagA-positive subjects predisposes to susceptibility to DU.

Gholamin M.,Avicenna Research Institute | Bazi A.,Zabol University of Medical Sciences | Abbaszadegan M.R.,Mashhad University of Medical Sciences
Current Opinion in Hematology | Year: 2015

Purpose of review Idiopathic CD4+ lymphocytopenia (ICL) is defined by the reduction of the main lymphocyte subtype in peripheral blood and CD4+ T cells below 300/μl in the absence of any secondary known causes of lymphopenia, including viral causes. The present review aims to state the latest available data on clinical, pathological and therapeutic aspects related to ICL, published from 1990 to 2014. The last observed clinical presentation and complications of ICL patients are described. The latest findings and possible mechanisms involved in the development of ICL features are included in the present review; however, pathogenesis of ICL has remained mainly obscured. Finally, recent therapeutic efforts considered in ICL patients are discussed. Recent findings In spite of the serious complications ICL has on the patients' quality of life, data on clinical, etiopathological and therapeutic behavior for ICL are very limited. On one side, an abnormal blood cell count may be the sole presentation; however, occurrence of disseminated malignant tumors is not uncommon in patients. Recent findings highlight the role of cytokines, especially interleukin-2, on features such as phenotype severity and responsiveness of the condition to therapy. In addition, some studies have suggested that a defect in hematopoietic stem cells may be involved in disease progression, an idea that is supported by the success of bone marrow transplantation in acquiring persistent remissions in ICL patients. Summary ICL is a hematologic condition of increasing importance due to its diverse clinical and pathological spectrum. Molecular studies have shown the presence of mutations involved in lymphocyte development as potential factors that may contribute to ICL occurrence. ICL patients could present either with common infections or really serious malignant conditions. The role of cytokines, especially interleukin-2, has emerged as one of the main possible mechanisms involved in clinical and pathological behavior of ICL. Today, the main therapeutic approaches are controlling life-threatening infections and underlying disorders along with efforts to cure ICL through rising CD4+ cell counts using cytokine interventions and transplantation. © 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins.

Khazaei M.,Isfahan University of Medical Sciences | Barmaki B.,Zabol University of Medical Sciences
Journal of Biomedicine and Biotechnology | Year: 2012

Introduction. In this study, we investigated the role of exogenous NO donor, sodium nitroprusside (SNP), on hemodynamic responses and survival rate during decompensated hemorrhagic shock in normotensive and hypertensive rat. Methods. Male wistar rats were divided into normotensive and hypertensive groups (n = 12 each). Then, the animals were subjected to decompensated hemorrhagic shock by withdrawing blood until the mean arterial pressure (MAP) reached to 40mmHg. After the shock period, the animals were randomly assigned to SNP-treated (0.5mg/kg) and control groups (n = 6 each). MAP and heart rate (HR) were monitored throughout the experiment and 60 min after the administration of drug. Serum NO concentrations were measured. The survival rate was counted during next 72h. Results. Infusion of SNP caused no significant changes in MAP and HR in normotensive and hypertensive animals. Hemorrhagic shock increased serum NO concentration and SNP administration reduced serum NO concentration in either normotensive or hypertensive groups. Survival counts during 72h after experiment did not improve by SNP administration, and there were no significant differences between normotensive and hypertensive groups. Conclusion. SNP administration cannot improve hemodynamic responses and survival count during decompensated hemorrhagic shock in normotensive and hypertensive animals. © 2012 Majid Khazaei and Babak Barmaki.

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