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Liang K.,University of Sichuan | Liang K.,Chongqing Medical University | Zhong G.,University of Sichuan | Yin J.,Yuxi Municipal Hospital of Traditional Chinese Medicine | And 3 more authors.
Archives of Orthopaedic and Trauma Surgery | Year: 2011

A 40-year-old woman had her right extremity avulsed at the proximal upper arm level and the wrist and hand of her left extremity irretrievably injured in a traffic accident. The right distal forearm was surgically amputated and replanted onto the stump of the left distal forearm. New strategy for nerve repair was applied and the function recovery of the cross-replanted hand was favorable. We thought that cross-extremity replantation was indicated when the patient suffered from bilateral total or subtotal amputation at different levels and orthotopic replantation was impossible. © 2010 Springer-Verlag. Source


Xu Z.-G.,Yuxi Municipal Hospital of Traditional Chinese Medicine | Yang J.,Yuxi Municipal Hospital of Traditional Chinese Medicine | Lu Z.-P.,Yuxi Municipal Hospital of Traditional Chinese Medicine | Sun Y.-H.,Yuxi Municipal Hospital of Traditional Chinese Medicine | And 6 more authors.
Journal of Sichuan University (Medical Science Edition) | Year: 2012

Objective To study the effects of gold belt (GB), a Chinese Herbal, on behavioral changes and brain derived neutrophic factor (BDNF) expression and N-methyl-D-aspartic acid (NMDA) receptor level in rats subjected to spinal cord injury (SCI). Methods Adult male SD rats were randomly divided into three groups, 1 Sham group; 2 Spinal cord injury group (SCI group); 3 Spinal cord injury followed with gold belt treatment (gold belt 50 mg/(kg • d), intragastric gavage once daily for 7 days) group (GB group). The Basso, Beattie and Bresnahan (BBB) locomotor scale method was performed to evaluate the hindlimb motor function in the days 0,3, 10 and 28. After 13 days, 8 rats in each group were treated with 1% sodium pentobarbital (30 mg/kg), myoloid tissue in T10 position was taken and stored in liquid nitrogen to detect NMDA receptor affinity and maximum binding amount (Bmax) with radioligand binding assay. After 28 days, rats were sacrificed and the spinal cords were harvested for immunohistochemistry to observe the localization of BDNF in the ventral and dorsal horn of the spinal cord. Results After spinal cord contusion, GB resulted in a significant increase on the number of BDNF positive neurons compared with traumatic group, and increased BBB score and decreased NMDA receptor were also found in GB group. Whereas decreased BDNF expression, NMDA receptor affininty (Kd) were observed in traumatic injury group. Conclusion The gold belt treatment could effectively improve motor function, increase expression of BDNF, reduce the level of NMDA receptors in SCI rats. These data suggested that the gold belt played a role in the neuroplasticity after spinal cord injury. Source


Yang J.,Yuxi Municipal Hospital of Traditional Chinese Medicine | Xu Z.-G.,Yuxi Municipal Hospital of Traditional Chinese Medicine | Rong R.,University of Sichuan | Lu Z.-P.,Yuxi Municipal Hospital of Traditional Chinese Medicine | And 6 more authors.
Journal of Sichuan University (Medical Science Edition) | Year: 2012

Objective To study the effect of methylprednisolone on spinal cord injury rats' neural behavior and the expression of brain derived neurotrophic factor (BDNF) and N-methyl-D-aspartic acid (NMDA). Methods To establish rat model of spinal cord injury (SCI), the rats were randomly divided into sham operation group, SCI group and methylprednisolone group (n=16 in each group). Eight rats were used for the behavioral assessment and BDNF measurement,the other eight animals was for the NMDA receptor test in each group. Within 8 h spinal cord contusion, methylprednisolone (50 mg/kg) was injected for methylprednisolone group, then after that the intramuscular injection of methylprednisolone was per day reduction in 10 mg/kg, till 5 days. By using immunohistochemical staining, the distribution of BDNF in the spinal cord and positive cell localization was observed and the number of positive cells were counted. The NMDA receptor affinity (Kd) and maximum binding amount (Bmax) were measured with [ 3H] MK-801 radioligand method, and the rat hind limb functional was also evaluated with BBB score analysis. Results Both the number of BDNF positive cells and the BBB score in methylprednisolone group was significant higher than that of SCI group; While increased receptor affinity (Kd) and decreased Bmax for NMDA receptor in methylprednisolone group was seen less than in SCI group ( P < 0.05).Conclusion Methylprednisolone Can Improve the Function of Rat Hind Limb, Increase BDNF Level and Decreased NMDA Receptor Expression after Spinal Cord Injury. Source

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