Yurun Group

Nanjing, China

Yurun Group

Nanjing, China
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Nikoo M.,State Key Laboratory of Food Science and Technology | Nikoo M.,Jiangnan University | Benjakul S.,Prince of Songkla University | Ehsani A.,Urmia University | And 7 more authors.
Journal of Functional Foods | Year: 2014

The peptide Pro-Ala-Gly-Tyr (PAGT) was isolated from Amur sturgeon skin gelatin and its antioxidant activity and cryoprotective effect in Japanese sea bass (Lateolabrax japonicus) mince was investigated. PAGT showed scavenging activity against DPPH, ABTS and hydroxyl radicals with no metal chelating activity. At a level of 25ppm, PAGT prevented lipid oxidation in minced fish, but at higher levels it was ineffective. Low-field nuclear magnetic resonance (LF 1H NMR) detected three water pools in mince in the range of 1-10, 10-100 and 100-300 microsecond (ms), respectively. PAGT influenced water distribution in the mince. Mince with PAGT (25ppm) had the highest T21 population, corresponding to a lower amount of water in T22 population after 6 freeze/thaw cycles (P<0.05). Myosin and actin denaturation decreased in the presence of PAGT. Lipid oxidation in mince was impeded when PAGT was incorporated at 25ppm as evidenced by the lower hydroperoxides from Fourier transform infrared (FTIR) spectra. © 2014 Elsevier Ltd.


Nikoo M.,Jiangnan University | Benjakul S.,Prince of Songkla University | Ocen D.,Jiangnan University | Yang N.,Jiangnan University | And 3 more authors.
Journal of Applied Ichthyology | Year: 2013

To expand the usefulness of cultured Amur sturgeon, Acipenser schrenckii, its skin was used to explore the production of gelatin. After acetic acid pre-treatment (0.05 m for 3 h), gelatin was extracted at temperatures of 50 or 70°C for 1 or 6 h. Gelatin yield ranged from 9.42 to 12.47% (wet weight basis) (P < 0.05). With increasing extraction time and temperature, the content of imino acids (proline + hydroxyproline), gel strength and L*-value (lightness) decreased, while the a*-value (redness) and b*-value (yellowness) of gelatin gel increased (P < 0.05). Electrophoretic analysis revealed that α-chains and β-chains were predominant components in all extracted gelatins. Higher molecular weight proteins (γ-chain) were also observed. Gelling and melting temperatures of gelatin were 13.6-14.6°C and 20.3-22.6°C, respectively. Circular dichroism (CD) spectra and Fourier transform infrared (FTIR) spectroscopy revealed the triple helix loss in gelatin (A1235 (AIII)/A1451 < 1). Extraction conditions caused secondary structure changes in the gelatin. More likely due to the differences in the culture water temperature, gelatin exhibited gelling and melting temperatures intermediate between those of cold- and warm-water fish gelatins. The obtained gelatin can be used in food products or in the production of bioactive compounds. © 2013 Blackwell Verlag GmbH.


Hao L.,Jiangnan University | Duan N.,Jiangnan University | Wu S.,Jiangnan University | Xu B.,Yurun Group | Wang Z.,Jiangnan University
Analytical and bioanalytical chemistry | Year: 2015

A novel chemiluminescent aptasensor for the highly sensitive detection of chloramphenicol (CAP) in milk was successfully developed using biotinylated CAP aptamer-functionalized magnetic nanoparticles (MNPs) as capture probes and thiolated hybridized complementary strand-modified N-(4-aminobutyl)-N-ethylisoluminol (ABEI)-functionalized flower-like gold nanostructures (AuNFs) as signal probes. P-iodophenol (PIP) was also added to form an ABEI-H2O2-PIP steady-state chemiluminescence (CL) system. Based on a competitive format, the CL intensity was negatively correlated with the concentration of CAP in the range of 0.01-0.20 ng/mL and the detection limit was 0.01 ng/mL in buffer and 1 ng/mL in milk. The proposed method was successfully applied to measure CAP in milk samples and compared to a commercial ELISA method. The high sensitivity of AuNFs, excellent selectivity and stability of aptamers, and good overall stability of the chemiluminescent bioassay with magnetic separation make them a promising approach for the detection of small molecular illegal additives. Additionally, the high sensitivity, easy operation, and good reproducibility exhibited by the stable chemiluminescent bioassay demonstrate its applicability for the trace detection of CAP in applications, such as animal husbandry.


Xu J.,Jiangnan University | Zhao W.,Jiangnan University | Ning Y.,Jiangnan University | Jin Z.,Jiangnan University | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

The effects of spring dextrin on amylose recrystallization were investigated by wide-angle X-ray diffraction (WXRD) and differential scanning calorimetry (DSC). Recrystallinity of amylose was reduced in terms of adding SD7, SD9, or SD11. Alternatively, SD 3 or SD5 accelerated the degree of crystallinity. DSC data were analyzed using the Avrami equation and confirmed the results of WXRD. Finally, molecular dynamic simulation was adapted to predict the behavior of polymers in water, and the results showed that the small spring dextrins disturbed amylose retrogradation by inhibiting or altering amylose-amylose interaction. © 2012 American Chemical Society.


Xu J.,Jiangnan University | Zhao W.,Jiangnan University | Ning Y.,Jiangnan University | Bashari M.,Jiangnan University | And 7 more authors.
Carbohydrate Polymers | Year: 2013

Food grade biopolymers, such as dextrin, have been suggested as a technological solution for the controlled delivery of health promoting substances. The main focus of this work is to improve the stability of poly-unsaturated fatty acids (PUFAs) and controlled release by encapsulating with helical spring dextrin (SD). The encapsulation was formed between SD with a DP of 62 and α-linolenic acid (ALA) or linoleic acid (LA) at 60 °C and characterized by WXRD, DSC, TGA and SEM. Under conditions which simulated the human environment of the gastrointestinal system, 21.7% and 18.5% of SD-ALA and SD-LA were released, respectively. A molecular dynamics simulation indicated that the space of helix cavity for ALA-SD complex was larger than that for LA-SD complex. This research work supports the idea that these complexes not only can improve the stability of ALA and LA, but also can achieve the targeted delivery of functional lipids or other bioactive components to the small intestine. © 2012 Elsevier Ltd.


Xu J.,Jiangnan University | Fan X.,Jiangnan University | Ning Y.,Jiangnan University | Wang P.,Jiangnan University | And 4 more authors.
Food Hydrocolloids | Year: 2013

The influence of spring dextrin (SD) on the gelatinized starch retrogradation was investigated by wide X-ray diffraction (WXRD), differential scanning calorimetry (DSC) and molecular dynamic (MD) simulation. Different wheat and corn starch gels were prepared with the addition of SD3, SD5, SD7 or SD9. WXRD results showed that recrystallinity of wheat and corn starch gels was reduced with the addition of SD7 or SD9. Alternatively, SD3 or SD5 accelerated recrystallinity. Enthalpies of retrograde wheat and corn starch gels were analyzed using the Avrami equation and confirmed the WXRD results. Finally, MD simulation was adapted to predict the interaction of SD and starch fraction in water, and the results showed that the SD disturbed starch retrogradation by altering starch fraction-starch fraction interaction. Based on the obtained results of WXRD, DSC and MD, it was concluded that the addition of SDs significantly influences starch long-term retrogradation. © 2013 Elsevier Ltd.


Duan N.,Jiangnan University | Xu B.,Yurun Group | Wu S.,Jiangnan University | Wang Z.,Yurun Group
Analytical Sciences | Year: 2016

This paper presents a sensitive and convenient visual methodology for Salmonella typhimurium detection using gold nanoparticles (AuNPs) as colorimetric probes and magnetic nanoparticles (MNPs) as concentration elements. In the protocol, the aptamers were first immobilized onto the surface of AuNPs and MNPs, respectively. Then, S. typhimurium were added into the above solution and incubated for 45 min. During the incubation, aptamer on the surface of nanoparticles could specifically bind to the target and form a MNPs-aptamer-S. typhimurium-aptamer-AuNPs sandwich structure complex. In a magnetic field, the formed complexes were easily separated from the solution, resulting in a fading of the AuNPs suspension and a decrease of the ultraviolet visible (UV/Vis) signal. The assay shows a linear response toward S. typhimurium concentration through a range of 25 to 105 cfu/mL, and the detection limit was improved to 10 cfu/mL. The applicability of the bioassay in real food samples was also investigated; the results were consistent with the experimental results obtained from plate-counting methods. It is believed that the developed aptasensor will broaden the application in bioassays. © The Japan Society for Analytical Chemistry.


PubMed | China Rural Technology Development Center, Yurun Group and Jiangnan University
Type: | Journal: Biosensors & bioelectronics | Year: 2015

Salmonella typhimurium and Staphylococcus aureus are most common causes of food-associated disease. A Raman based biosensor was developed for S. typhimurium and S. aureus detection simultaneously. The biosensor was based on nanoparticles enhanced Raman intensity and the specific recognition of aptamer. The Raman signal probe and the capture probe are built. Gold nanoparticles (GNPs) modified with Raman molecules (Mercaptobenzoic acid and 5,5-Dithiobis(2-nitrobenzoic acid)) and aptamer are used as the signal probe for S. typhimurium and S. aureus, respectively. Fe3O4 magnetic gold nanoparticles (MGNPs) immobilized with both aptamer of S. typhimurium and S. aureus are used as the capture probe. When S. typhimurium and S. aureus are added in the reaction system, the capture probe will capture the target bacteria through the specific binding effect of aptamer. And then the signal probe will be connected to the bacteria also by the effect of aptamer to form the sandwich like detection structure. The Raman intensified spectrum was measured to quantify S. typhimurium and S. aureus. Under optimal conditions, the SERS intensity of MBA at 1582 cm(-1) are used to measure S. typhimurium (y=186.4762+704.8571x, R(2)=0.9921) and the SERS intensity of DNTB at 1333 cm(-1) are used to measure S. aureus (y=135.2381+211.4286x, R(2)=0.9946) in the range of 10(2)-10(7) cfu mL(-1). The LOD is 35 cfu mL(-1) for S. aureus and 15 cfu mL(-1) for S. typhimurium. This method is simple and rapid, results in high sensitivity and specificity, and can be used to detect actual samples.


Wang X.,Jiangnan University | Huang Y.,Xihua University | Wu S.,Jiangnan University | Duan N.,Jiangnan University | And 2 more authors.
International Journal of Food Microbiology | Year: 2016

Foodborne illnesses caused by Staphylococcus aureus and Salmonella typhimurium are common public health issues worldwide, affecting both developing and developed countries. In this study, aptamers labeled with multicolor lanthanide-doped time-resolved fluorescence (TRFL) nanoparticles were used as signal probes, and immobilized by Fe3O4 magnetic nanoparticles were used as the capture probes. The signal probes were bonded onto the captured bacteria by the recognition of aptamer to form the sandwich-type complex. Under the optimal conditions, TRFL intensity at 544 nm was used to quantify S. typhimurium (y = 10,213 × − 12,208.92, R2 = 0.9922) and TRFL intensity at 615 nm for S. aureus (y = 4803.20 × − 1933.87, R2 = 0.9982) in the range of 102–105 CFU/ml. Due to the magnetic separation and concentration of Fe3O4 nanoparticles, detection limits of the developed method were found to be 15, 20 CFU/ml for S. typhimurium and S. aureus, respectively. The application of this bioassay in milk was also investigated, and results were consistent with those of plate-counting method. Therefore, this simple and rapid method owns a great potential in the application for the multiplex analysis in food safety. © 2016 Elsevier B.V.


PubMed | Yurun Group and Jiangnan University
Type: Evaluation Studies | Journal: Analytical and bioanalytical chemistry | Year: 2015

A novel chemiluminescent aptasensor for the highly sensitive detection of chloramphenicol (CAP) in milk was successfully developed using biotinylated CAP aptamer-functionalized magnetic nanoparticles (MNPs) as capture probes and thiolated hybridized complementary strand-modified N-(4-aminobutyl)-N-ethylisoluminol (ABEI)-functionalized flower-like gold nanostructures (AuNFs) as signal probes. P-iodophenol (PIP) was also added to form an ABEI-H2O2-PIP steady-state chemiluminescence (CL) system. Based on a competitive format, the CL intensity was negatively correlated with the concentration of CAP in the range of 0.01-0.20ng/mL and the detection limit was 0.01ng/mL in buffer and 1ng/mL in milk. The proposed method was successfully applied to measure CAP in milk samples and compared to a commercial ELISA method. The high sensitivity of AuNFs, excellent selectivity and stability of aptamers, and good overall stability of the chemiluminescent bioassay with magnetic separation make them a promising approach for the detection of small molecular illegal additives. Additionally, the high sensitivity, easy operation, and good reproducibility exhibited by the stable chemiluminescent bioassay demonstrate its applicability for the trace detection of CAP in applications, such as animal husbandry.

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