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Nanjing, China

Xu J.,Jiangnan University | Zhao W.,Jiangnan University | Ning Y.,Jiangnan University | Jin Z.,Jiangnan University | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

The effects of spring dextrin on amylose recrystallization were investigated by wide-angle X-ray diffraction (WXRD) and differential scanning calorimetry (DSC). Recrystallinity of amylose was reduced in terms of adding SD7, SD9, or SD11. Alternatively, SD 3 or SD5 accelerated the degree of crystallinity. DSC data were analyzed using the Avrami equation and confirmed the results of WXRD. Finally, molecular dynamic simulation was adapted to predict the behavior of polymers in water, and the results showed that the small spring dextrins disturbed amylose retrogradation by inhibiting or altering amylose-amylose interaction. © 2012 American Chemical Society.

Hao L.,Jiangnan University | Duan N.,Jiangnan University | Wu S.,Jiangnan University | Xu B.,Yurun Group | Wang Z.,Jiangnan University
Analytical and bioanalytical chemistry | Year: 2015

A novel chemiluminescent aptasensor for the highly sensitive detection of chloramphenicol (CAP) in milk was successfully developed using biotinylated CAP aptamer-functionalized magnetic nanoparticles (MNPs) as capture probes and thiolated hybridized complementary strand-modified N-(4-aminobutyl)-N-ethylisoluminol (ABEI)-functionalized flower-like gold nanostructures (AuNFs) as signal probes. P-iodophenol (PIP) was also added to form an ABEI-H2O2-PIP steady-state chemiluminescence (CL) system. Based on a competitive format, the CL intensity was negatively correlated with the concentration of CAP in the range of 0.01-0.20 ng/mL and the detection limit was 0.01 ng/mL in buffer and 1 ng/mL in milk. The proposed method was successfully applied to measure CAP in milk samples and compared to a commercial ELISA method. The high sensitivity of AuNFs, excellent selectivity and stability of aptamers, and good overall stability of the chemiluminescent bioassay with magnetic separation make them a promising approach for the detection of small molecular illegal additives. Additionally, the high sensitivity, easy operation, and good reproducibility exhibited by the stable chemiluminescent bioassay demonstrate its applicability for the trace detection of CAP in applications, such as animal husbandry.

Xu J.,Jiangnan University | Fan X.,Jiangnan University | Ning Y.,Jiangnan University | Wang P.,Jiangnan University | And 4 more authors.
Food Hydrocolloids | Year: 2013

The influence of spring dextrin (SD) on the gelatinized starch retrogradation was investigated by wide X-ray diffraction (WXRD), differential scanning calorimetry (DSC) and molecular dynamic (MD) simulation. Different wheat and corn starch gels were prepared with the addition of SD3, SD5, SD7 or SD9. WXRD results showed that recrystallinity of wheat and corn starch gels was reduced with the addition of SD7 or SD9. Alternatively, SD3 or SD5 accelerated recrystallinity. Enthalpies of retrograde wheat and corn starch gels were analyzed using the Avrami equation and confirmed the WXRD results. Finally, MD simulation was adapted to predict the interaction of SD and starch fraction in water, and the results showed that the SD disturbed starch retrogradation by altering starch fraction-starch fraction interaction. Based on the obtained results of WXRD, DSC and MD, it was concluded that the addition of SDs significantly influences starch long-term retrogradation. © 2013 Elsevier Ltd.

Zhang H.,China Rural Technology Development Center | Ma X.,Jiangnan University | Liu Y.,Jiangnan University | Duan N.,Jiangnan University | And 3 more authors.
Biosensors and Bioelectronics | Year: 2015

Salmonella typhimurium and Staphylococcus aureus are most common causes of food-associated disease. A Raman based biosensor was developed for S. typhimurium and S. aureus detection simultaneously. The biosensor was based on nanoparticles enhanced Raman intensity and the specific recognition of aptamer. The Raman signal probe and the capture probe are built. Gold nanoparticles (GNPs) modified with Raman molecules (Mercaptobenzoic acid and 5,5'-Dithiobis(2-nitrobenzoic acid)) and aptamer are used as the signal probe for S. typhimurium and S. aureus, respectively. Fe3O4 magnetic gold nanoparticles (MGNPs) immobilized with both aptamer of S. typhimurium and S. aureus are used as the capture probe. When S. typhimurium and S. aureus are added in the reaction system, the capture probe will capture the target bacteria through the specific binding effect of aptamer. And then the signal probe will be connected to the bacteria also by the effect of aptamer to form the sandwich like detection structure. The Raman intensified spectrum was measured to quantify S. typhimurium and S. aureus. Under optimal conditions, the SERS intensity of MBA at 1582cm-1 are used to measure S. typhimurium (y=186.4762+704.8571x, R2=0.9921) and the SERS intensity of DNTB at 1333cm-1 are used to measure S. aureus (y=135.2381+211.4286x, R2=0.9946) in the range of 102-107cfumL-1. The LOD is 35cfumL-1 for S. aureus and 15cfumL-1 for S. typhimurium. This method is simple and rapid, results in high sensitivity and specificity, and can be used to detect actual samples. © 2015 Elsevier B.V.

Nikoo M.,State Key Laboratory of Food Science and Technology | Nikoo M.,Jiangnan University | Benjakul S.,Prince of Songkla University | Ehsani A.,Urmia University | And 7 more authors.
Journal of Functional Foods | Year: 2014

The peptide Pro-Ala-Gly-Tyr (PAGT) was isolated from Amur sturgeon skin gelatin and its antioxidant activity and cryoprotective effect in Japanese sea bass (Lateolabrax japonicus) mince was investigated. PAGT showed scavenging activity against DPPH, ABTS and hydroxyl radicals with no metal chelating activity. At a level of 25ppm, PAGT prevented lipid oxidation in minced fish, but at higher levels it was ineffective. Low-field nuclear magnetic resonance (LF 1H NMR) detected three water pools in mince in the range of 1-10, 10-100 and 100-300 microsecond (ms), respectively. PAGT influenced water distribution in the mince. Mince with PAGT (25ppm) had the highest T21 population, corresponding to a lower amount of water in T22 population after 6 freeze/thaw cycles (P<0.05). Myosin and actin denaturation decreased in the presence of PAGT. Lipid oxidation in mince was impeded when PAGT was incorporated at 25ppm as evidenced by the lower hydroperoxides from Fourier transform infrared (FTIR) spectra. © 2014 Elsevier Ltd.

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