Yunnan University of Traditional Chinese Medicine
Kunming, China

Yunnan University of Traditional Chinese Medicine , established in 1960 in Kunming, Yunnan, China, was among the second group of TCM universities set up nationwide. With more than 45 years of history, the university is now able to award master degrees and has set up two campuses in sequence, the Bai Ta campus and Guan Shang campus. Presently the university is constructing a third campus which is located in Cheng Gong near Kunming, which will cover an area of over 546,940 square meters and the total construction area is 270,000 square meters, which will be finished in the autumn of 2008. The university has produced ten thousands doctorates of various levels and specialties. The university has more than 10,000 students, and 1,200 staff members.The university focuses on the education of undergraduate and post-graduates whilst enrolling pre-undergraduates and students of higher vocational education. Currently, the university grants medical degrees in two programs together with Guangzhou University of Traditional Chinese Medicine. It also grants first-rank discipline master degrees in three programs, and secondary discipline master degrees in 16 programs including 40 research fields. The university also has 14 undergraduate specialties and research fields ranging from medicine, science, technology and management, 9 pre-undergraduate and higher vocational education programs. Wikipedia.

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Yang J.,National University of Singapore | Chen J.,Yunnan University of Traditional Chinese Medicine | Li Z.,Innovis
Australian Journal of Chemistry | Year: 2016

The discrimination of Pt-GG adducts by mismatch repair proteins, DNA damage-recognition proteins, and translation DNA polymerases was thought to be vital in determining the toxicity, efficacy, and mutagenicity of platinum anti-tumour drugs. Studies on cis-diammine-Pt-GG (from cisplatin and carboplatin) and trans-R,R-diaminocyclohexane (DACH)-Pt-GG indicated that these proteins recognized the differences in conformation and conformational dynamics of Pt-DNA complexes. However, the structural basis of enantiomeric DACH-Pt-GG forms is unclear. Molecular dynamics simulations results presented here reveal that the conformational dynamics between trans-R,R-DACH-Pt-GG, trans-S,S-DACH-Pt-GG, cis-DACH-Pt-GG and undamaged DNA are distinct and depend on the chirality of DACH though their major conformations are similar. Trans-DACH-Pt was found to be energetically favoured over cis-DACH-Pt to form DNA adducts. Moreover, oxaliplatin and its cis-DACH analogues were found to preferentially form hydrogen bonds on the 3′ side of the Pt-GG adduct, whereas the S,S-DACH-Pt preferred the 5′ side. A three-centre hydrogen bond formed between cis1-DACH-Pt and DNA was observed, and the differences in hydrogen bond formation are highly correlated with differences in DNA conformational dynamics. Based on these results, it is suggested that the different bioactivities of oxaliplatin and its enantiomeric analogues were controlled by the difference in hydrogen bonds formation dynamics between DNA and the Pt moiety. Our molecular dynamics approach was demonstrated to be applicable to the study of stereoisomer conformations of platinum-DNA model, thereby suggesting its potential application as a tool for the study and design of new effective platinum-based drugs. © 2016 CSIRO.

Zakus D.,Ryerson University | Gao C.,Yunnan University of Traditional Chinese Medicine
Family Medicine and Community Health | Year: 2016

Chinese governmental sectors have been investing lots of money in setting up new institutions for long-term care (LTC) in rural China to respond to the predicted increasing and urgent needs of the growing number of aging people. However, very few have identified that many ethnic minority groups have been enjoying their traditional home-based LTC systems, which are cost-effective and have been working really well for generations. This article reports on a study on LTC needs and provisions for the Dai, one of the ethnic minority groups in rural areas of southwest China, in Yunnan (Province). A randomized sample was drawn from 12 villages in Ruili, a county 752 km from Kunming, the capital city of Yunnan. Totally, 187 elderly persons were interviewed intensively. Activities of daily living were measured as the core indicator for understanding LTC. The research revealed that there is a significant disparity between the needs of the villagers and provisions from the different levels of government. There is an urgent need to reexamine the allocation of public resources for the aged. Traditional culture and practices of caring for the elderly, and practices in LTC of different ethnic groups, must be carefully considered. © 2016 Family Medicine and Community Health.

Ge F.,Kunming University of Science and Technology | Chen C.,Kunming University of Science and Technology | Liu D.,Kunming University of Science and Technology | Zhao S.,Yunnan University of Traditional Chinese Medicine
Food Analytical Methods | Year: 2014

Adulteration of walnut oil with sunflower oil is a major issue for the walnut oil industry. In this paper, the potential use of total synchronous fluorescence (TSyF) spectra to differentiate walnut oil from sunflower oil and synchronous fluorescence spectra combined with multivariate analysis to assess the adulteration of walnut oil is demonstrated. TSyF spectra were acquired by varying the excitation wavelength in the region 250-700 nm and the wavelength interval (Δλ) in the region from 10 to 100 nm. TSyF contour plots for walnut oil, in contrast to sunflower oil, show an extra fluorescence region in the excitation wavelength lower than 280 nm. Fifty-one oil mixtures were prepared by adulterating walnut oil with sunflower oil at varying levels (0-100 %). The partial least-squares regression model was used for the quantification of adulteration using wavelength intervals of 20, 40, 60 and 80 nm. This technique is useful for the detection of sunflower oil in walnut oil at levels down to 0.3 % (v/v) in just 2.5 min using an 80-nm wavelength interval. © 2013 Springer Science+Business Media New York.

Zhao S.-D.,CAS Kunming Institute of Botany | Zhao S.-D.,Yunnan University of Traditional Chinese Medicine | Shen L.,CAS Kunming Institute of Botany | Shen L.,Chinese Academy of Sciences | And 2 more authors.
Current Organic Chemistry | Year: 2011

With the development of separation materials, more and more complex natural products with poly-stereogenic centers are obtained. However, due to small quantity of the isolated compounds, or no crystal formed, or complex 2D NMR correlation signals, absolute configuration identification for these compounds becomes difficult. Now, computational methods provide researchers many benefits in structural determinations for chiral natural products. Computational methods include (1) optical rotation (OR) and its dispersion (ORD) computations; (2) electronic circular dichroism (ECD or CD) calculations; (3) computations of determinant of matrix for stereogenic centers. In methods (1) to (2), the mostly used quantum theory contained density functional theory (DFT) with different basis sets, e.g. B3LYP/aug-cc-pVDZ//B3LYP/6-31G(d). Effects from solvents on OR value can be investigated using different corresponding software. In the cases, optimizations performed in the corresponding solution are helpful to obtain the correct predictions although it is not usual at present time. Matrix model as one of the mathematic methods is different from quantum methods, which is of easily being understood and effective in absolute configuration assignment for acyclic chiral compounds. In this review, necessary theory basis for the three methods are simply introduced for modern readers, the focus of which is the application of these methods in natural product chemistry by various examples reported in different journals from 1998 to the end of April of 2010 except for matrix method to the May of 2010 based on an experimental chemist's viewpoints. © 2011 Bentham Science Publishers Ltd.

Dai Y.,Third Affiliated Hospital of Kunming Medical College | Cui J.,Second Affiliated Hospital of Kunming Medical College | Cun Y.,Third Affiliated Hospital of Kunming Medical College | Shi A.,Yunnan University of Traditional Chinese Medicine
Journal of Surgical Research | Year: 2012

Background: In the liver, eNOS appears to have a central role in protecting against ischemia/reperfusion (I/R) injury. We hypothesized that tetrahydrobiopterin (BH4) would protect livers subjected to I/R injury by coupling with eNOS. Methods: Chinese Kun Ming (KM) mice were subjected to 60 min of 70% hepatic ischemia 30 min after the administration of BH4 or saline. After reperfusion, survival was evaluated. The histologic appearance and ALT, BH4, nitrite/nitrate, 8-isoprostane, and eNOS protein expression levels were measured. Results: The 1-wk survival rate was 66.67% in the BH4 group and 33.33% in the saline group. The serum ALT values in the BH4 group 1, 3, 6, 12, and 24 h after reperfusion were significantly lower than those of the saline group. A histologic examination of the liver revealed only a small necrotic area in the BH4 group as opposed to massive necrosis in the saline group. The percentage values of the hepatic necrotic area 24 h after reperfusion were significantly less for the BH4 group than for the saline group. The nitrite/nitrate levels in the liver tissue were significantly increased by ∼2-fold in the BH4 group compared with the saline group. The free radical indicator 8-isoprostane was reduced approximately 50% in the BH4 group compared with the saline group. Western blotting showed that the level of eNOS protein between the groups was not significantly different. Conclusions: BH4 significantly improved the survival rate by reducing liver failure. This was supported by the histologic findings, and the mechanism was explored. According to the results, we suggest that BH4 prevents liver damage from I/R injury by attenuating reactive oxygen species and increasing NO synthesis, and might provide a novel and promising therapeutic option for preventing I/R injury. © 2012 Elsevier Inc. All rights reserved.

Fei Z.-X.,Yunnan Normal University | Zhang M.,Yunnan University of Traditional Chinese Medicine | Xie S.-M.,Yunnan Normal University | Yuan L.-M.,Yunnan Normal University
Electrophoresis | Year: 2014

Metal-organic frameworks (MOFs) have received great attention because of their unusual properties and fascinating structures in separation sciences. However, to the best of our knowledge, there has been no attempt to utilize chiral MOFs as stationary phases in packed-CEC. Here, a chiral MOF [In3O(obb)3(HCO2)(H2O)]·solvent (4,4′-oxybisbenzoic acid) was explored as the chiral stationary phase in packed-CEC for separation of chiral compounds and isomers. The fabricated [In3O(obb)3(HCO2)(H2O)]·solvent packed capillary columns gave fast enantioseparation of (±)-hydrobenzoin, (±)-1-phenyl-1,2-ethanediol, and clenbuterol within 3 min in CEC. Besides, the baseline separations of nitrophenol isomers within 6 min were also achieved. The RSDs for the retention time of run-to-run, day-to-day, and column-to-column reproducibility were 1.51-3.63, 1.83-3.98, and 3.42-5.66%, respectively. These results demonstrate that chiral MOFs are promising for enantioseparation in CEC. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Wang W.,Yunnan University of Traditional Chinese Medicine | He Y.,Yunnan University of Traditional Chinese Medicine | Lin P.,Yunnan University of Traditional Chinese Medicine | Li Y.,Yunnan University of Traditional Chinese Medicine | And 4 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacology relevance Raw and processed Polygoni Multiflori Radix (PMR and PMRP) are used in the prevention and treatment of non-alcoholic fatty liver disease (NAFLD), hyperlipidemia or related diseases. In our previous research, 2, 3, 5, 4′-tetrahydroxy-stilbene-2-O-β-d-glucoside (TSG) displayed the most important role in the total cholesterol (TC) lowering effect among all the chemical constituents of Polygonum multiflorum. Emodin and physcion displayed more favorable triglyceride (TG) reducing effects than TSG. However, there are few researches focus on the approach and mechanism of how do Polygonum multiflorum exhibit good lipid regulation activity. The targeted sites of active substances of Polygonum multiflorum are still not clearly elucidated. This research pays close attention to how major chemical components of Polygonum multiflorum affect the TC and TG contents in liver cells. Materials and methods In this research, a sensitive, accurate and rapid in vitro model, steatosis hepatic L02 cell, was used to explore target sites of active chemical substances of Polygonum multiflorum for 48 h. Steatosis hepatic L02 cell was exposed to emodin, physcion and TSG, respectively. The contents of four key enzymes in the pathway of synthesis and decomposition of TC and TG were investigated after exposure. Meanwhile, the contents of lipid transfer protein were also tested. The diacylgycerol acyltransferase 1 (DGAT1) controlled the biosynthesis of TG from free fatty acids while 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMG-CoA reductase) limited the biosynthesis of TC. Hepatic triglyceride lipase (HTGL) and cholesterol 7α-hydroxylase (CYP7A) played the key role in the lipolysis procedure of TG and TC. Results The synthesis of TC and TG in steatosis L02 cells were apparently increased in the model group compared to the control group. Intracellular contents of HMG-CoA reductase and DGAT1 increased 32.33% and 56.52%, while contents of CYP7A and HTGL decreased 21.61% and 47.37%. Emodin, physcion and TSG all showed down-regulation effects on HMG-CoA reductase, while up-regulation effects on CYP7A. The most remarkable effect on HMG-CoA reductase was found on emodin. Emodin could reduce the DGAT1 content from 438.44±4.51 pg/mL in model group to 192.55±9.85 pg/mL (100 μm). The content of HTGL in 300 μm physcion group was 3.15±0.15 U/mL, which was more significantly effective than the control, lovastatin and fenofibrate group. Conclusions TSG could raise the content of CYP7A and then promote the lipolysis of cholesterol. Moreover, TSG also showed the best LDL-reducing effect. Emodin could inhibit HMG-CoA reductase and DGAT1, which were key enzymes in the synthesis of TC and TG. Physcion increased the content of HTGL, and then could boost the lipolysis of triglyceride. At the same time, physcion showed the best VLDL-reducing effect. In view of the above conclusions, we contributed the lipid regulation activity to an overall synergy of TSG, emodin and physcion. © 2014 Elsevier Ireland Ltd.

Li J.B.,Yunnan University of Traditional Chinese Medicine
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2013

By taking "lumbar disc herniation", Chinese medicine", "acupuncture and moxibustion" as key words Chinese articles about acupuncture for lumbar disc herniation in recent 10 years were searched in three major Chinese databases of Wan fang database, VIP database and CNKI. Totally 546 relative articles were retrieved. After the elimination of invalid articles, 173 were included as valid articles with 49 main acupoints. The hierarchical clustering statistical method was used to analyze the possible rules among the former 20 acupoints. It turned out that there were 10 most common used acupoints on Bladder Meridian [Geshu (BL 17), Xiaochangshu (BL 27), Shenshu (BL 23), Dachangshu (BL 25), Pangguangshu (BL 28), Guanyuanshu (BL 26), Weizbong (BL 40), Zhibian (BL 54), Chengshan (BL 57), Kunlun (BL 60)], 2 on Gallbladder Meridian [Huantiao (GB 30), Yanglingquan (GB 34)], 2 on Governor Vessel [Yaoyangguan (GV 3), Shuigou (GV 26)], 2 on Stomach Meridian [Zusanli (ST 36), Juliao (ST 3)], 2 on Spleen Meridian [Sanyinjiao (SP 6), Xuehai (SP 10)], and the rest were extra points (Huatuo Jiaji) and Ashi points, so a conclusion could be drawn that the most common used acupoints were Bladder Meridian acupoints and supplemented by Gallbladder Meridian, Governor Vessel, Stomach Meridian, Spleen Meridian, extra points and Ashi points. The selected acupoints were most located on the lumbosacral region, leg and fewer located on the face, back and local part.

Yang L.-J.,Yunnan University of Traditional Chinese Medicine | Dai Z.-C.,Kunming University
Wuli Xuebao/Acta Physica Sinica | Year: 2012

The effects of correlated time between noises on the stability of unstable state in the Logistic system are investigated. Using the steepest-descent approximation, the analytic expression for the mean first-passage time from unstable state to stable state is derived. The numerically calculated results indicate that the additive noise, the multiplicative noise and the correlated time between the additive and the multiplicative noises weaken the stability of unstable state, but the correlation between the additive and the multiplicative noises enhances the stability of unstable state. © 2012 Chinese Physical Society.

Yang L.-J.,Yunnan University | Yang L.-J.,Yunnan University of Traditional Chinese Medicine | Lv F.,Yunnan University of Traditional Chinese Medicine | Mei D.-C.,Yunnan University
Physica A: Statistical Mechanics and its Applications | Year: 2015

The effects of periodic force on the stability of the metastable state in logistic model are investigated. The expression of the mean first-passage time (MFPT) from the metastable state to the stable state is derived. Based on the expression, the effects of periodic force on the MFPT were analyzed. The results indicate that: (i) For the case of the multiplicative noise induced transition, the multiplicative noise and the periodic force weaken the stability of the metastable state; (ii) For the case of the additive noise induced transition, the stability of the metastable state is weakened by the additive noise when the periodic force takes value of the first half cycle, while the stability of the metastable state is enhanced by the additive noise when the periodic force takes value of the second half cycle; (iii) For the case of the correlation between the multiplicative and the additive noise induced transition, the correlation between the multiplicative and the additive noises and the periodic force weaken the stability of the metastable state. © 2015 Elsevier B.V.

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