Yunnan Tobacco Research Institute

Yuxi, China

Yunnan Tobacco Research Institute

Yuxi, China
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Jia R.,Southwest University | Geng M.,Southwest University | Li Y.,Sichuan Liangshan Agricultural Research Institute | Duan Y.,Yunnan Tobacco Research Institute | And 3 more authors.
Tobacco Science and Technology | Year: 2016

In order to develop efficient, nontoxic and cost-effective bio-control agents against tobacco black shank, antagonism, pot and field experiments were carried out to study the effects of fermentation broth produced from a self-isolated Pythium oligandrum (strain CQ2010) on the growth and control of black shank for flue-cured tobacco, and its acute toxicity to mice. The results of antagonism experiment indicated that Pythium oligandrum broth (POB) significantly inhibited mycelial growth and spore germination of in vitro Phytophora nicotiana Breda de Haan. Comparing to the blank control (without POB), the biomass of tobacco seedlings grown in pots increased by 58.91% after POB application. Similarly, chlorophyll contents in the leaves, root vigor and nitrogen, phosphorus and potassium uptake were increased by POB, and the effect of POB treatment was better than that of P. oligandrum spores. POB also promoted the activities of enzymes, such as peroxidase and catalase in tobacco leaves, furthermore induced tobacco plants' biochemical reactions for disease resistance. The effects were beneficial to eliminating reactive oxygen and H2O2 and alleviating the damage caused by diseases. The incidence of tobacco plants decreased by 42.95%-65.77% via POB treatment before or after pathogen inoculation, and the relative control efficacy reached 46.99%-57.32%. The content of malondialdehyde in tobacco leaves was also significantly reduced by POB application, and the damage of pathogen to cell membrane was relieved. In field experiments, botrytis control-efficacy of POB reached 47.91% and tobacco leaf yield was increased by 44.47% and output value by 36.33%. The mice tested showed that there was no significant weight difference between mice fed with pure water (the control) and gastric lavage with high doses of POB, no abnormality in appearance and behavior was observed in tested mice, and their heart, liver, kidney, lung and other organs had no pathologic changes. In summary, the preliminary results demonstrated the potential of POB as an animal safe bio-control agent. In the tests shown, POB controls botrytis efficiently, promotes the growth of flue-cured tobacco and increases the yield and output value of tobacco leaves. © 2016, Editorial Office of Tobacco Science and Technology. All right reserved.


Zhang S.,Yunnan Tobacco Research Institute
Tobacco Science and Technology | Year: 2016

In order to evaluate the effects of drying methods on the chemical contents of tobacco leaves, green tobacco leaves were dried by three experimental methods: freeze-drying, hot air drying and kill-enzyme torrefaction. The chemical contents of selected main leaf components after drying were analyzed. The result showed that the main chemical components in the dried tobacco leaves were more variable by hot air drying and kill-enzyme torrefaction; especially, the pigment contents and polyphenol levels decreased sharply. However, the pigment contents and polyphenol levels in the leaves dried by the freeze-drying method were between several to dozens of times higher than those in leaf treated by the kill-enzyme torrefaction. Therefore, to obtain real variation of pigment and polyphenol levels in tobacco leaves during the curing process, freeze-drying method was recommended. © 2016, Editorial Office of Tobacco Science and Technology. All right reserved.


Liu Y.,Hubei Key Laboratory of Economic Forest Germplasm Improvement | Lu X.,Yunnan Tobacco Research Institute
Journal of Phytopathology | Year: 2012

Leaf samples were collected from four spider lily and tobacco plants with tospovirus-like symptoms in Yunnan province. The nucleocapsid ORFs of the four isolates were obtained by RT-PCR, cloning and sequencing of the amplified products. Nucleotide sequences of the obtained amplicons were more than 90% identical to that of Calla lily chlorotic spot virus (CCSV) isolates deposited in the GenBank database. The virus induced systematic leaf necrosis, chlorotic lesions, rugosity and deformation on mechanically inoculated experimental hosts. This is the first report of CCSV in mainland China, and also on spider lily and tobacco. © 2012 Blackwell Verlag GmbH.


Yao Z.-Y.,Yunnan Reascend Tobacco Technology Group Co. | Xu J.-C.,Yunnan Tobacco Research Institute | Shen Y.,Yunnan Reascend Tobacco Technology Group Co. | Huang H.-Q.,Yunnan Reascend Tobacco Technology Group Co.
Zhongguo Zaozhi Xuebao/Transactions of China Pulp and Paper | Year: 2016

In order to study the effect of permeability of tipping paper on cigarette burning temperature and the component of the mainstream smoke, the smoke of cigarettes with tipping papers having different permeability was analyzed. The infrared camera was used to measure burning temperature of cigarette using tipping papers with different permeabilities. The results indicated that tipping paper permeability had a great influence on suction burning temperature. With the increase of tipping paper permeability,the third suction burning temperature and the average peak temperature values were dropped obviously. With permeability of tipping paper increased from 100 CU to 500 CU, the third puff burning temperature dropped by 115℃, average peak temperature dropped by 87℃, but the change of smoldering temperature was not obvious. In addition, smoldering temperature was significantly lower than puff temperature. With the increase of tipping paper permeability, tar, CO and nicotine in cigarette mainstream smoke were significantly decreased linearly, puff count was increased. After using 500 CU tipping paper, tar was reduced by 50.6%, CO was reduced by 64.6%, nicotine was reduced by 42.9%, puff count was increased by 22.5%. © 2016, China National Pulp and Paper Research Institute(CNPPRI). All right reserved.


Yang Z.,Yunnan University | Yu Z.,Yunnan University | Lei L.,Yunnan Tobacco Research Institute | Xia Z.,Yunnan Tobacco Research Institute | And 3 more authors.
Journal of Asia-Pacific Entomology | Year: 2012

Trichoderma is an important biocontrol agent that produces metabolites harmful to nematodes. We investigated the volatile organic compounds (VOCs) of Trichoderma sp. YMF 1.00416 and examined their abilities to kill nematodes. Chemical investigations of the VOCs from this strain led to the isolation and identification of three metabolites: a new compound, 1β-vinylcyclopentane-1α,3α-diol (1) and two known metabolites, 6-pentyl-2H-pyran-2-one (2) and 4-(2-hydroxyethyl)phenol (3). Nematicidal activity assays showed that compound 2 was nematicidal, and killed > 85% of Panagrellus redivivus, Caenorhabditis elegans, and Bursaphelenchus xylophilus in 48. h at 200. mg/L in a 2. mL vial. Our results will help identify new nematicides. © 2012 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.


Luo S.-L.,Yunnan University | Li G.-H.,Yunnan University | Liu F.-F.,Yunnan University | Lei L.-P.,Yunnan University | And 2 more authors.
Natural Product Research | Year: 2012

A fungal strain named YLF-14 was isolated from the leaf of Altingia yunnunensis. Based on the sequence at the internal transcribed spacer (ITS) region, the strain was identified as an Aspergillus sp. A new sesquiterpene 5,8a-dimethyl-3,4,4a,5,6,7,8,8a-octahydronaphthalene-1,2,5-trimethanol (1) and a known compound 12-N-methylcyclo-(L-tryptophyl-L-phenylalanyl) (2) were isolated from the culture of this strain. Bioassay experiments showed that the two compounds had no obvious effect on the growth of tested bacteria and nematodes. © 2012 Copyright Taylor and Francis Group, LLC.


Wang Q.,Huazhong Agricultural University | Huang J.,Huazhong Agricultural University | He F.,Henan Agricultural University | Cui K.,Huazhong Agricultural University | And 3 more authors.
Field Crops Research | Year: 2012

"Super" hybrid rice varieties have been widely grown in farmers' fields since the late 1990s in China due to their high yield potential. The objective of this study was to compare head rice yield and related grain quality traits between a "super" hybrid variety, Liangyoupeijiu (LYPJ), and an ordinary hybrid variety, Shanyou 63 (SY63), across a wide range of nitrogen (N) rates. The two hybrid varieties were grown under field conditions with seven and six N treatments in 2004 and 2005, respectively, in Hubei province, China. Grain yield, head rice yield, head rice percentage, chalky rice rate and other grain quality traits were measured in the two years. Although the difference in grain yield was small between the two varieties, the average head rice yield across N treatments for LYPJ was 34% and 24% higher than that for SY63 in 2004 and 2005, respectively. Head rice percentage was significantly affected by N rates, but LYPJ maintained higher head rice percentage than SY63 across a wide range of N rates. The higher head rice percentage of LYPJ was associated with its lower chalky rice rate compared with SY63. LYPJ had higher amylose content, greater gel consistency, and lower protein content than SY63, suggesting a better palatability of cooked rice for LYPJ than for SY63. © 2012 Elsevier B.V.

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