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Liu Y.,Hubei Key Laboratory of Economic Forest Germplasm Improvement | Lu X.,Yunnan Tobacco Research Institute
Journal of Phytopathology | Year: 2012

Leaf samples were collected from four spider lily and tobacco plants with tospovirus-like symptoms in Yunnan province. The nucleocapsid ORFs of the four isolates were obtained by RT-PCR, cloning and sequencing of the amplified products. Nucleotide sequences of the obtained amplicons were more than 90% identical to that of Calla lily chlorotic spot virus (CCSV) isolates deposited in the GenBank database. The virus induced systematic leaf necrosis, chlorotic lesions, rugosity and deformation on mechanically inoculated experimental hosts. This is the first report of CCSV in mainland China, and also on spider lily and tobacco. © 2012 Blackwell Verlag GmbH.

Yang Z.,Yunnan University | Yu Z.,Yunnan University | Lei L.,Yunnan Tobacco Research Institute | Xia Z.,Yunnan Tobacco Research Institute | And 3 more authors.
Journal of Asia-Pacific Entomology | Year: 2012

Trichoderma is an important biocontrol agent that produces metabolites harmful to nematodes. We investigated the volatile organic compounds (VOCs) of Trichoderma sp. YMF 1.00416 and examined their abilities to kill nematodes. Chemical investigations of the VOCs from this strain led to the isolation and identification of three metabolites: a new compound, 1β-vinylcyclopentane-1α,3α-diol (1) and two known metabolites, 6-pentyl-2H-pyran-2-one (2) and 4-(2-hydroxyethyl)phenol (3). Nematicidal activity assays showed that compound 2 was nematicidal, and killed > 85% of Panagrellus redivivus, Caenorhabditis elegans, and Bursaphelenchus xylophilus in 48. h at 200. mg/L in a 2. mL vial. Our results will help identify new nematicides. © 2012 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.

Luo S.-L.,Yunnan University | Li G.-H.,Yunnan University | Liu F.-F.,Yunnan University | Lei L.-P.,Yunnan University | And 2 more authors.
Natural Product Research | Year: 2012

A fungal strain named YLF-14 was isolated from the leaf of Altingia yunnunensis. Based on the sequence at the internal transcribed spacer (ITS) region, the strain was identified as an Aspergillus sp. A new sesquiterpene 5,8a-dimethyl-3,4,4a,5,6,7,8,8a-octahydronaphthalene-1,2,5-trimethanol (1) and a known compound 12-N-methylcyclo-(L-tryptophyl-L-phenylalanyl) (2) were isolated from the culture of this strain. Bioassay experiments showed that the two compounds had no obvious effect on the growth of tested bacteria and nematodes. © 2012 Copyright Taylor and Francis Group, LLC.

Wang Q.,Huazhong Agricultural University | Huang J.,Huazhong Agricultural University | He F.,Henan Agricultural University | Cui K.,Huazhong Agricultural University | And 3 more authors.
Field Crops Research | Year: 2012

"Super" hybrid rice varieties have been widely grown in farmers' fields since the late 1990s in China due to their high yield potential. The objective of this study was to compare head rice yield and related grain quality traits between a "super" hybrid variety, Liangyoupeijiu (LYPJ), and an ordinary hybrid variety, Shanyou 63 (SY63), across a wide range of nitrogen (N) rates. The two hybrid varieties were grown under field conditions with seven and six N treatments in 2004 and 2005, respectively, in Hubei province, China. Grain yield, head rice yield, head rice percentage, chalky rice rate and other grain quality traits were measured in the two years. Although the difference in grain yield was small between the two varieties, the average head rice yield across N treatments for LYPJ was 34% and 24% higher than that for SY63 in 2004 and 2005, respectively. Head rice percentage was significantly affected by N rates, but LYPJ maintained higher head rice percentage than SY63 across a wide range of N rates. The higher head rice percentage of LYPJ was associated with its lower chalky rice rate compared with SY63. LYPJ had higher amylose content, greater gel consistency, and lower protein content than SY63, suggesting a better palatability of cooked rice for LYPJ than for SY63. © 2012 Elsevier B.V.

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