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Fang J.,Kunming University of Science and Technology | Chen D.,Yunnan Tobacco Quality Supervision and Inspection Station | Chen C.,Kunming University of Science and Technology | Ge F.,Kunming University of Science and Technology | And 3 more authors.
Food Science and Biotechnology

An indirect enzyme-linked immunosorbent assay (ELISA) capable of detecting walnut proteins in commercial products was developed. Polyclonal antibodies against soluble walnut protein were used. Specific epitopes of walnut protein were analyzed for cross activity with peanut, soybean, sesame, rice, wheat, jujube, and defatted milk protein. Epitopes of walnut proteins had no cross activity. The indirect ELISA was highly specific for soluble walnut proteins. Recovery values from walnut protein solutions ranged from 88.47 to 115%, and low coefficients of variation (<10%) indicated good repeatability. Intra and inter-assay precisions were <6 and <7 %, respectively. The limit of detection was 10 ng/mL of soluble walnut protein. The method was applied to detect some commercial food products in China and the results showed that indirect ELISA could be promisingly used to quantify adulteration of walnut processed foods. © 2015, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht. Source

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