Time filter

Source Type

Qi L.-M.,Yunnan University of Traditional Chinese Medicine | Qi L.-M.,Yunnan Academy of Agricultural Sciences | Qi L.-M.,Yunnan Technical Center for Quality of Chinese Materia Medica | Zhang J.,Yunnan Academy of Agricultural Sciences | And 9 more authors.
Analytical Letters | Year: 2017

Gentiana rigescens is a famous herbal medicine in China for treatment of convulsion, rheumatism, and jaundice. Here, the infrared determination of gentiopicroside, swertiamarin, sweroside, and loganic acid in G. rigescens from different areas and varieties was presented for the first time. Reference information for the iridoids were obtained by high-performance liquid chromatography. Partial least squares was used to characterize the relationship between spectra matrix and concentration vector for the determination of the analytes. For determination of gentiopicroside, the appropriate performance of partial least squares model was acquired with coefficient of determination of calibration and coefficient of determination of prediction values of 0.965 and 0.868. The root mean square error of estimation (RMSEE), root mean square error of cross validation (RMSECV), root mean square error of prediction (RMSEP), and residual predictive deviation (RPD) values were 2.612, 5.292, 5.239 mg g−1, and 2.701, respectively, based on the first derivative and multiplicative scatter correction. For determination of the total iridoids, the best results were obtained using the coefficient of determination of calibration and coefficient of determination of prediction of 0.943 and 0.834, RMSEE, RMSECV, RMSEP and RPD of 3.896, 7.536, 6.543 mg g−1 and 2.438, respectively, based on the first derivative. Both models were reliable and robust. The results demonstrated that infrared spectroscopy provided a rapid, low-cost tool to monitor the quality of G. rigescens by the determination of the iridoids. © 2017 Taylor & Francis.


Falandysz J.,University of Gdansk | Zhang J.,Yunnan Academy of Agricultural Sciences | Zhang J.,Yunnan Technical Center for Quality of Chinese Materia Medica | Zalewska T.,National Water Research Institute
Environmental Science and Pollution Research | Year: 2017

This study, for the first time, presents the results of activity concentration determinations for 137Cs and 40K in a high number (21 species, 87 composite samples, and 807 fruiting bodies) of mushrooms of the genus Boletus from across Yunnan in 2011–2014 and Sichuan (Boletus tomentipes) using high-resolution high-purity germanium detector. Activity concentrations of 137Cs demonstrated some variability and range from <4.4 to 83 ± 3 Bq kg−1 dry biomass in caps and from <3.8 to 37 ± 3 Bq kg−1 dry biomass in stipes, and of 40K, respectively, from 420 ± 41 to 1300 ± 110 and from 520 ± 61 to 1300 ± 140 Bq kg−1 dry biomass. No significant variations were observed regarding 137Cs and 40K activity concentrations among the same Boletus species from different sampling sites. No activity concentrations from 134Cs were detected in any mushrooms. Internal dose rates estimated were from intake of 1 kg of mushrooms per annum for 137Cs range for species and regions from around <0.0031 to 0.047 ± 0.003 μSv, while those for 40K were from around 0.22 ± 0.04 to 1.2 ± 0.1 μSv. The overall intake of 137Cs was low since low contamination was found in Boletus species. © 2017 The Author(s)


Wang X.,Yunnan Agricultural University | Wang X.,Yunnan Academy of Agricultural Sciences | Liu H.,Yunnan Agricultural University | Zhang J.,Yunnan Academy of Agricultural Sciences | And 4 more authors.
Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes | Year: 2017

The heavy metal contents (Co, Cu, Fe, Mn, Ni, and Zn) of eight species of wild edible mushrooms from China were determined. The analyses were performed using inductively coupled plasma atomic emission spectrophotometry after microwave digestion. The contents of Co, Cu, Fe, Mn, Ni, and Zn in caps of mushroom samples were 0.7–7.2, 16.2–70.4, 371–1315, 12.5–29.8, 7.1–58.5, and 77.8–187.4 mg kg−1 dry matter (dm), respectively, while considerable differences were found to be 1.8–25.9, 9.8–36.3, 288–6762, 13.3–103.9, 5.9–78.7, and 38.7–118 mg kg−1 dm for stipes. The results indicated that higher levels of Co, Fe, and Ni were found in the mushrooms samples analyzed. Zinc and manganese levels were similar to previous reports, whereas Cu was lower than literature values. Correlation analysis suggested that significant correlations were found between the minerals determined and the greatest amount of contamination is associated with Co, Mn, Ni, and Fe. The results of this study indicate that heavy metal contents in mushroom species are mainly related to the mineral resources of sampling sites. © 2017 Taylor & Francis Group, LLC.


Falandysz J.,University of Gdansk | Saba M.,University of Gdansk | Liu H.-G.,Yunnan Agricultural University | Li T.,Yuxi Normal University | And 8 more authors.
Environmental Science and Pollution Research | Year: 2016

This study aimed to investigate and discuss the occurrence and accumulation of mercury in the fruiting bodies of wild-growing fungi (Macromycetes) collected from montane forests in two regions of southwestern China with differences in soil geochemistry, climate and geographical conditions. Fungal mycelia in soils of the subalpine region of the Minya Konka (Gongga Mountain) in Sichuan and in the highlands of Yunnan efficiently accumulated mercury in fruiting bodies (mushrooms). The examined sites in Yunnan with highly mineralized red and yellow soils showed Hg contents ranging from 0.066 to 0.28 mg kg−1 dry biomass (db) which is roughly similar to the results obtained for samples collected from sites with dark soils relatively rich in organic matter from a remote, the subalpine region of Minya Konka. Due to the remoteness of the subalpine section of Minya Konka, as well as its elevation and climate, airborne mercury from long-range transport could be deposited preferentially on the topsoil and the Hg levels determined in soil samples taken beneath the fruiting bodies were up to 0.48 mg kg−1 dry matter. In Yunnan, with polymetallic soils (Circum-Pacific Mercuriferous Belt), Amanita mushrooms showed mercury in caps of fruiting bodies of up to 7.3 mg kg−1 dry biomass. Geogenic Hg from the mercuriferous belt seems to be the overriding source of mercury accumulated in mushrooms foraged in the regions of Yunnan, while long-range atmospheric transport and subsequent deposition are the mercury sources for specimens foraged in the region of Minya Konka. © 2016 The Author(s)


Chu B.,Yunnan Academy of Agricultural Sciences | Shi Y.,Yunnan Academy of Agricultural Sciences | Tian H.,Yunnan Technical Center for Quality of Chinese Materia Medica | Li W.,Yunnan Academy of Agricultural Sciences | Wang Y.,Yunnan Academy of Agricultural Sciences
Journal of Analytical Methods in Chemistry | Year: 2015

Gentisides are a class of chemical compounds which is considered as potential therapeutic substance for treatment of neurodegenerative disorders. The heat reflux extraction conditions were optimized for seven kinds of gentisides from the root and rhizome of Gentiana rigescens Franch. ex Hemsl. by employing response surface method. Based on univariate test, a Box-Behnken design (BBD) was applied to the survey of relationships between response value (gentisides yield) and independent variables which were chosen from various extraction processes, including extraction temperature, extraction time, and solvent-material ratio. The optimized conditions for this extraction are as follows: extraction time of 3.40 h, extraction temperature of 74.33°C, and ratio of solvent to raw material of 10.21: 1 mL/g. Verification assay revealed that the predicted value (99.24%) of extraction parameters from this model was mainly conformed to the experimentally observed values (98.6 1 ± 0.6 1). © 2015 Bowen Chu et al.


Pan Y.,Yunnan Academy of Agricultural Sciences | Pan Y.,Yunnan Technical Center for Quality of Chinese Materia Medica | Zhang J.,Yunnan Academy of Agricultural Sciences | Zhang J.,Yunnan Technical Center for Quality of Chinese Materia Medica | And 6 more authors.
Journal of the Science of Food and Agriculture | Year: 2016

BACKGROUND: Macamides with a benzylalkylamide nucleus are characteristic and major bioactive compounds in the functional food maca (Lepidium meyenii Walp). The aim of this study was to explore variations in macamide content among maca from China and Peru. Twenty-seven batches of maca hypocotyls with different phenotypes, sampled from different geographical origins, were extracted and profiled by liquid chromatography with ultraviolet detection/tandem mass spectrometry (LC-UV/MS/MS). RESULTS: Twelve macamides were identified by MS operated in multiple scanning modes. Similarity analysis showed that maca samples differed significantly in their macamide fingerprinting. Partial least squares discriminant analysis (PLS-DA) was used to differentiate samples according to their geographical origin and to identify the most relevant variables in the classification model. The prediction accuracy for raw maca was 91% and five macamides were selected and considered as chemical markers for sample classification. CONCLUSION: When combined with a PLS-DA model, characteristic fingerprinting based on macamides could be recommended for labelling for the authentication of maca from different geographical origins. The results provided potential evidence for the relationships between environmental or other factors and distribution of macamides. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry


Pan Y.,Yunnan Academy of Agricultural Sciences | Pan Y.,Yunnan Technical Center for Quality of Chinese Materia Medica | Zhang J.,Yunnan Academy of Agricultural Sciences | Zhang J.,Yunnan Technical Center for Quality of Chinese Materia Medica | And 6 more authors.
Phytochemical Analysis | Year: 2016

Introduction: Gentianaceae species which widely occur all over the world are used as folk medicine and raw food material with bitter properties. Although comparative analysis on metabolites in several Gentianaceae species has been reported, metabolic similarities used for chemotaxonomic studies are not yet clear. Objective: To systematically characterise the variations of holistic metabolome and characteristic metabolites (iridoid glycosides and phenols) in nine Gentianaceae species from western China. Methodology: Fourier transform infrared (FT-IR) spectroscopy was applied to determine the variations of holistic metabolome. A targeted metabolic profiling using liquid chromatography with tandem mass spectrometry (LC–MS/MS) was established for determination of seven characteristic metabolites and identification of their derivatives. Both FT-IR and LC–MS/MS data were subjected to chemometrics analysis for exploring variations in iridoid glycosides and phenols within these species. Results: Holistic metabolome in genera Gentiana and Swertia was largely different. Diversity of the biosynthetic pathway of iridoid glycosides was also observed in these species. Principal component analysis (PCA) showed a clear separation according to infrageneric classifications of genus Gentiana. Some secondary metabolites, such as mangiferin, rhodenthoside A-C, isoorientin, isovitexin, amarogentin, and swertianolin would serve as potential chemotaxonomic markers to differentiate Gentianaceae species. Furthermore, the accumulation of the six major metabolites seems to depend on geographical regions in Sect. Monopodiae and Sect. Cruciata. Conclusions: The combination of LC–MS/MS and FT-IR would provide some potential evidence on chemotaxonomic studies of Gentianaceae. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.


Wu Z.,Yunnan Academy of Agricultural Sciences | Wu Z.,Yunnan Technical Center for Quality of Chinese Materia Medica | Wu Z.,Yunnan University of Traditional Chinese Medicine | Zhang J.,Yunnan Academy of Agricultural Sciences | And 5 more authors.
Journal of Natural Medicines | Year: 2016

Paris Polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz (“Dian Chonglou” in Chinese) is a famous herbal medicine in China, which is usually well known for activities of anti-cancer, hemolysis, and cytotoxicity. In this study, Fourier transform infrared (FT-IR) spectroscopy coupled with principal component analysis (PCA) and partial least-squares regression (PLSR) was applied to discriminate samples of P. polyphylla var. yunnanensis harvested in different years and determine the content of polyphyllin I, II, VI, and VII in P. polyphylla var. yunnanensis. Meanwhile, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to study the dynamic changes of P. polyphylla var. yunnanensis harvested in different years (4, 5, 7, 8, 9, 12, and 13 years old). According to the UPLC-MS/MS result, the optimum harvest time of P. polyphylla var. yunnanensis is 8 years, due to the highest yield of four active components. By the PCA model, P. polyphylla var. yunnanensis could be exactly discriminated, except that two 8-year-old samples were misclassified as 9-year-old samples. For the prediction of polyphyllin I, II, VI, and VII, the quantitative results are satisfactory, with a high value for the determination coefficient (R2) and low values for the root-mean-square error of estimation (RMSEE), root-mean-square error of cross-validation (RMSECV), and root-mean-square error of prediction (RMSEP). In conclusion, FT-IR combined with chemometrics is a promising method to accurately discriminate samples of P. polyphylla var. yunnanensis harvested in different years and determine the content of polyphyllin I, II, VI, and VII in P. polyphylla var. yunnanensis. © 2016 The Japanese Society of Pharmacognosy and Springer Japan


Li Y.,Yunnan Academy of Agricultural Sciences | Li Y.,Yunnan Technical Center for Quality of Chinese Materia Medica | Li Y.,Yunnan University of Traditional Chinese Medicine | Zhang J.,Yunnan Academy of Agricultural Sciences | And 6 more authors.
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2016

A quality assessment system comprised of a tandem technique of ultraviolet (UV) spectroscopy and ultra-fast liquid chromatography (UFLC) aided by multivariate analysis was presented for the determination of geographic origin of Wolfiporia extensa collected from five regions in Yunnan Province of China. Characteristic UV spectroscopic fingerprints of samples were determined based on its methanol extract. UFLC was applied for the determination of pachymic acid (a biomarker) presented in individual test samples. The spectrum data matrix and the content of pachymic acid were integrated and analyzed by partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA). The results showed that chemical properties of samples were clearly dominated by the epidermis and inner part as well as geographical origins. The relationships among samples obtained from these five regions have been also presented. Moreover, an interesting finding implied that geographical origins had much greater influence on the chemical properties of epidermis compared with that of the inner part. This study demonstrated that a rapid tool for accurate discrimination of W. extensa by UV spectroscopy and UFLC could be available for quality control of complicated medicinal mushrooms. © 2016 Elsevier B.V. All rights reserved.


Pan Y.,Yunnan Academy of Agricultural Sciences | Pan Y.,Yunnan Technical Center for Quality of Chinese Materia Medica | Zhang J.,Yunnan Academy of Agricultural Sciences | Zhang J.,Yunnan Technical Center for Quality of Chinese Materia Medica | And 6 more authors.
Food Analytical Methods | Year: 2015

Macamides with benzylalkylamides structure are a group of characteristic constituents isolated from functional food Maca (Lepidium meyenii Walp.). Previous study demonstrated that macamides are secondary amides, the accumulation of which is associated with drying process. In this study, a rapid method based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) is developed for the simultaneous detection and determination of macamides in Maca. Validation parameters including linearity, limit of detection, limit of quantification, accuracy, precision, repeatability, and stability were all within the required limits. Seven major macamides are determined by the UPLC method with detection wavelength at 205 nm in 12 min. Based on the fragmentation pattern of standard compounds, four fragment ions (m/z 91, 108, 121, and 138) are selected as diagnostic ions for detection of macamides through precursor ion acquisition mode. Additionally, comparative analysis is performed to examine the effects of different drying processes (air-drying, oven-drying, freeze-drying, and steaming) on Maca (whole hypocotyls and pieces) for macamides. Whole hypocotyls with oven-drying showed the highest content of macamides (2.528 mg/g), while freeze-drying and steaming significantly decrease the amount of macamides. The results provide a reliable method for analysis of macamides and extend previous work insights into the drying process of Maca. © 2015 Springer Science+Business Media New York

Loading Yunnan Technical Center for Quality of Chinese Materia Medica collaborators
Loading Yunnan Technical Center for Quality of Chinese Materia Medica collaborators