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Xin A.,Yunnan Animal Science and Veterinary Institute | Zhu M.,Yunnan Provincial Research Center for Veterinary Biological Products | Hu Q.,Yunnan Animal Science and Veterinary Institute | Miao H.,Yunnan Animal Science and Veterinary Institute | And 4 more authors.
Virologica Sinica | Year: 2014

An amino acid mutation (R127→I) in the 3A non-structural protein of an FMDV serotype Asia1 rabbit-attenuated ZB strain was previously found after attenuation of the virus. To explore the effects of this mutation on viral replication and infection, the amino acid residue isoleucine (I) was changed to arginine (R) in the infectious cDNA clone of the rabbit-attenuated ZB strain by sitedirected mutagenesis, and the R127-mutated virus was rescued. BHK monolayer cells and suckling mice were inoculated with the R127-mutated virus to test its growth property and pathogenicity, respectively. The effects of the R127 mutation on viral replication and virulence were analyzed. The data showed that there was a slight difference in plaque morphology between the R127-mutated and wild-type viruses. The growth rate of the mutated virus was lower in BHK-21 cells and its virulence in suckling mice was also attenuated. This study indicates that the R127 mutation in 3A may play an important role in FMDV replication in vitro and in pathogenicity in suckling mice. © 2014, Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg.


Xin A.,Yunnan Animal Science and Veterinary Institute | Zhu M.,Yunnan Provincial Research Center for Veterinary Biological Products | Peng Z.,Yunnan Provincial Research Center for Veterinary Biological Products | Hu Q.,Yunnan Animal Science and Veterinary Institute | And 4 more authors.
International Journal of Genomics | Year: 2014

The molecular basis of attenuation of foot-and-mouth disease virus (FMDV) serotype Asia1 ZB strain remains unknown. To understand the genetic changes of attenuation, we compared the entire genomes of three different rabbit-passaged attenuated ZB strains (ZB/CHA/58(att), ZBRF168, and ZBRF188) and their virulent parental strains (ZBCF22 and YNBS/58). The results showed that attenuation may be brought about by 28 common amino acid substitutions in the coding region, with one nucleotide point mutation in the 5′-untranslated region (5′-UTR) and another one in the 3′-UTR. In addition, a total of 21 nucleotides silent mutations had been found after attenuation. These substitutions, alone or in combination, may be responsible for the attenuated phenotype of the ZB strain in cattle. This will contribute to elucidation of attenuating molecular basis of the FMDV ZB strain. © 2014 Aiguo Xin et al.

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