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Cao Z.,Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science | Cao Z.,Yunnan Agricultural University | Pan H.,Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science | Tong H.,Yunnan Agricultural University | And 7 more authors.
Annals of Microbiology | Year: 2015

This study aimed to investigate the probiotic potential of Pediococcus pentosaceus L1 isolated from paocai, a Chinese fermented vegetable. In vitro analysis revealed that P. pentosaceus L1 had the capability to tolerate simulated gastrointestinal juices. Adhesion of P. pentosaceus L1 to HT-29 intestinal epithelial cells (IEC) was also observed. L1 was sensitive to ampicillin, gentamycin, kanamycin, strepomycin, clindamycin, tetracycline and chloramphenicol. L1 showed effective inhibition against Escherichia coli, Salmonella typhimurium, and Shigella flexneri. P. pentosaceus L1 also exhibited the abilities of auto-aggregation and co-aggregation with Shigella flexneri. Pre-treatment of HT-29 IEC with P. pentosaceus L1 prior to tumor necrosis factor-alpha (TNFα) challenge down-regulated the expression of pro-inflammatory genes, such as IL8, CCL20, CXCL10, and CXCL1. The level of IL-8 released in culture supernatant of TNFα-challenged HT-29 IEC was reduced by strain L1, confirming the observed decrease in TNFα-induced IL-8 mRNA expression. These results indicate the probiotic potential of P. pentosaceus L1, and that this strain could be used to produce functional foods. © 2015 Springer-Verlag Berlin Heidelberg and the University of Milan

Gu Z.,Yunnan Agricultural University | Yang S.,Yunnan Agricultural University | Yang S.,Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science | Leng J.,Yunnan Agricultural University | And 7 more authors.
Applied Animal Behaviour Science | Year: 2016

Shade shelter is widely used to reduce heat load for farm animals, but little research focus on the cooling effects for Dehong buffalo calves. The aim of this experiment was to evaluate the impacts of shade cooling on the physiological and behavioural parameters for Dehong buffalo calves. Shade with 99% blockage of solar radiation was covered on steel frame with a 2.5. m height above the loafing area floor (shaded). The loafing area of the control group was the same as the shaded group, apart from no shade shelter above the loafing area (non-shaded). Twenty-four Dehong buffalo calves were randomly and averagely allocated to the non-shaded and shaded groups. Six Dehong buffalo calves were loose housed in a barn, and they had access to loafing area. The results showed that the average daily air temperature of the loafing area under shade was lower 1.5. °C than that of the loafing area without shade shelter. No difference in rectal temperature was found between the non-shaded and shaded calves (P >. 0.05), but respiration rate of the non-shaded group in noon (12:00. h, 31.6 breaths) and evening (18:00. h, 28.5 breaths) was greater than for calves in shaded group (26.1 and 25.3 breaths, respectively) (P <. 0.05). When ambient temperature exceeded 30. °C, shaded calves spent much of their time (21.7%) lying in the loafing area, and no calf in non-shaded group preferred to lie in the loafing area with strong solar radiation (P <. 0.05). The use of shade for calves was affected by thermal environment, and the use of shade was greater (41.4%) in hot weather (above 30. °C) than that of the warm weather (22-29. °C) (P <. 0.05). In conclusion, the provision of shade above the loafing area is conducive to improving thermal environment for Dehong buffalo calves in subtropical area characterised with high ambient temperature coupled with high humidity in summer. © 2016.

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