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Tian F.,Johns Hopkins Medical Institutions | Wang J.,Chinese Academy of Sciences | Huang Y.,Chinese Academy of Sciences | Guo X.,Mianyang Blood Center | And 6 more authors.
Vox Sanguinis | Year: 2010

Background and Objectives On May 12, 2008, a severe earthquake hit Sichuan province in China. A post-earthquake survey was conducted to study the earthquake's effect on blood donor behaviour and stress at three blood centres at varying distances from the epicentre. Materials and Methods A questionnaire was developed to assess donor post-traumatic stress disorders (PTSD) and attitudes toward giving blood. Responses were compared by centre and donor characteristics using multivariate logistic regression techniques. Results Of all 17 456 donors, the overall prevalence of PTSD was 13·2%. Donors who knew someone killed or injured by the earthquake were 2·1 times more likely to have PTSD than others (95% CI: 1·8-2·4). 85·2% of donors cited the earthquake as their reason for donating. 16·1% of donors felt it acceptable to be less honest about one's health history in an emergency. After adjusting for PTSD, geographic and demographic characteristics, the donors knowing someone killed or injured by the earthquake were 1·4 times (95% CI: 1·2-1·7) more likely to cite the earthquake as reason for donating, and 1·8 times (95% CI: 1·5-2·1) more likely to accept being less honest about one's health history in case of national emergency. Conclusions The psychological and behavioural impacts of the earthquake on blood donors extended far from the epicentre. After a disaster, it is important to emphasize that donors must be truthful on the donor questionnaire as some donors appear willing to be less than honest when they perceive an increased need for blood products. © 2010 International Society of Blood Transfusion.

Huang Y.,Chinese Academy of Sciences | Guo N.,Johns Hopkins Medical Institutions | Yu Q.,Westat Inc. | Lv Y.,Luoyang Blood Center | And 19 more authors.
Transfusion | Year: 2015

Background: Few studies were conducted on hepatitis B and C virus (HBV and HCV, respectively) risk factors among Chinese blood donors in recent years since voluntary donors replaced commercial donors. Study Design and Methods: A case-control survey was conducted in HBV- or HCV-positive and -negative donors from five blood centers in China between September 2009 and April 2011. Case status was defined by having a reactive result on Monolisa HBsAg Ultra (Bio-Rad) for HBV and Ortho anti-HCV EIA 3.0 (Johnson & Johnson) for HCV. Controls were randomly selected qualified blood donors matched to cases by donation month and blood center. Specific test-seeking, medical-related, and behavioral risk factors were compared by HBV and HCV status using chi-square tests or Fisher's exact tests with Bonferroni correction. Results: A total of 364 HBV cases, 174 HCV cases, and 689 controls completed the survey; response rates were 66.2, 47.3, and 82%, respectively. HCV-positive donors were significantly more likely to report having a blood transfusion history (23.4% vs. 3.0%, p < 0.0001) and ever living with a person with illegal drug injection (6.0% vs. 0.5%, p < 0.0001) than controls. Having intravenous and intramuscular injections in the past 12 months and ever having a tattoo are marginal risk factors for HCV (p values < 0.01). No specific risk factor for HBV was identified. Conclusion: History of previous transfusion and living with illegal drug users are risk factors for HCV infection among Chinese blood donors from five regions. Test-seeking behavior is not associated with HBV or HCV infections. © 2014 AABB.

Guo N.,Johns Hopkins University | Wang J.,Chinese Academy of Sciences | Ness P.,Johns Hopkins University | Yao F.,Yunnan Kunming Blood Center | And 14 more authors.
Vox Sanguinis | Year: 2012

Background On 12 May 2008, a severe earthquake struck Sichuan in China. Many people donated blood for the first time, leading us to question whether these donors might become repeat donors in the future. The return pattern of post-earthquake first-time donors (PEFTD) was compared with that of first-time donors (FTD) in a comparable period. Methods Demographic characteristics, transfusion-transmissible infection rates and 1-year return rates were compared between 5147 PEFTD (5/13-5/19, 2008) and 3176 FTD (5/13-5/19, 2009) from five Chinese blood centres using chi-squared tests. Adjusted logistic regression was used to detect earthquake effect on donor return. Results Post-earthquake first-time donors were more frequently between 26 and 45years, men, and better educated compared with the control group. Slightly higher but not statistically significant increased rates of hepatitis B virus surface antigen (HBsAg) (0·87% vs. 0·50%, P=0·054), hepatitisC virus (HCV) (0·70% vs. 0·63%, P=0·414), syphilis (0·9% vs. 0·7%, P=0·489) and lower rates of human immunodeficiency virus (HIV) (0·31% vs. 0·60%, P=0·078) reactivity were detected for PEFTD. The 1-year return rate for PEFTD was significantly lower than that of the controls (8·0% vs. 13·0%, P<0·001). After adjusting for demographic factors, donation volume and sites, the PEFTD were less likely to return in 1year than the controls (OR: 0·520; 95% CI: 0·442, 0·611). Conclusion Post-earthquake first-time donors may be less likely to donate again without continuing motivation strategies. Further studies on PEFTD's lack of motivation to return for donation are needed to design recruitment strategies to convert PEFTD to become repeat donors to continuously replenish the blood supply. © 2011 The Author(s). Vox Sanguinis © 2011 International Society of Blood Transfusion.

Pang W.,CAS Kunming Institute of Zoology | Zhang C.,Jiangsu University | Duo L.,Second Peoples Hospital | Zhou Y.-H.,CAS Kunming Institute of Zoology | And 5 more authors.
AIDS | Year: 2012

Objective: To investigate the subtype characterization of HIV-1 among IDUs in northern Myanmar. Design: A molecular epidemiological investigation was conducted among IDUs in Laza and Maizayang cities of northern Myanmar. Methods: A total of 83 HIV-1-positive serums were collected from consenting IDUs during June to August 2009. HIV-1 p17, pol, vif-env, C2V3 fragments were amplified and sequenced. Phylogenetic and bootscanning analyses were performed. Results: A very high proportion (86.1%) of HIV-1 intersubtype recombinants and very low proportion of subtypes B′ (3.8%), C (7.6%) and CRF01-AE (1.3%) were found in this HIV-infected IDUs cohort. These recombinants cover all four kinds of recombination forms formed among CRF01-AE, B and C. The B/C and CRF01-AE/B/C recombinants are the two most dominant recombinants, accounting for 54.4 and 42.6% of all cases, respectively, and indicating the ongoing generation of extensive and complex HIV-1 recombination among CRF01-AE, B′ and C in northern Myanmar. Intriguingly, most recombinants have different chimeric patterns from each other, forming 64 unique recombination forms (URFs) that are quite distinct from any previously identified circulating recombinant forms (CRFs) and URFs in Asia. Conclusion: The extremely high proportion of intersubtype recombinants, especially CRF01-AE/B′/C recombinants (42.6%), strongly suggests that northern Myanmar is a big forge for HIV-1 recombination among CRF01-AE, B′ and C. © 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins.

Zhu X.,National Health Research Institute | Zhu X.,Yunnan Kunming Blood Center | Yang T.,National Health Research Institute | Tan R.,National Health Research Institute | And 3 more authors.
Tissue Antigens | Year: 2015

HLA-B*58:66 differs from B*58:01:01 by a single-synonymous nucleotide at position 685 where G→T (codon 205 GCC→TCC, Ala>Ser). © 2015 John Wiley & Sons A/S.

Zhu X.,Yunnan Kunming Blood Center | Yang T.,Yunnan Kunming Blood Center | Yao F.,Yunnan Kunming Blood Center | Che Z.,Yunnan Kunming Blood Center | And 3 more authors.
Tissue Antigens | Year: 2014

A novel allele HLA-A*02:482 differs from the closest allele HLA-A*02:01:01:01 by a nucleotide change at codon 57. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

PubMed | Yunnan Kunming Blood Center and Peking Union Medical College
Type: Journal Article | Journal: HLA | Year: 2016

HLA-C*07:477 has one nonsynonymous nucleotide change from HLA-C*07:02:01:01 in codon 176, exon 3 (AAG>GAG).

PubMed | Yunnan Kunming Blood Center and Peking Union Medical College
Type: Journal Article | Journal: HLA | Year: 2016

Killer cell immunoglobulin-like receptor (KIR) genes are variably distributed among populations from distinct geographic areas and ethnic origins. We describe, for the first time, KIR gene diversity in 152 unrelated and healthy Drung individuals, as measured by sequence-specific polymerase chain reaction. All 16 known KIR genes were detected. Of these, the framework genes KIR2DL4, 3DL2, 3DL3, and 3DP1 were present in all individuals as expected, along with the non-framework genes KIR2DL1, 2DL3, and 2DP1. In contrast, KIR2DL2, 2DS2, and 2DS5 were unusually rare, suggesting that KIR gene distribution was relatively concentrated. Ten different KIR genotypes were found, of which the most common consisted of nine genes (KIR2DL1, 2DL3, 2DL4, 2DS4, 3DL1, 3DL2, 3DL3, 2DP1, and 3DP1) and accounted for 66.4% of participants. There were eight different haplotypes present, of which the A haplotype was the most common (81.9%). Principal components and dendrogram analysis confirmed that the Drung Chinese are most closely related to the Japanese, the Zhejiang Han, and the Yunnan Han. In conclusion, distinctive frequencies of KIR genes, haplotypes, and genotypes are observed in Chinese Drung.

PubMed | Yunnan Kunming Blood Center and Central South University
Type: Journal Article | Journal: HLA | Year: 2016

HLA-B*35:269 differs from HLA-B*35:01:01:01, by a single synonymous nucleotide at position 103.

PubMed | National Health Research Institute and Yunnan Kunming Blood Center
Type: Journal Article | Journal: HLA | Year: 2016

HLA-DRB1*12:48 differs from DRB1*12:09, by a single synonymous nucleotide at position 266.

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